EA007146B1 - Method and system for analysis of liquid medium characteristics in biological organisms - Google Patents

Method and system for analysis of liquid medium characteristics in biological organisms Download PDF

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Publication number
EA007146B1
EA007146B1 EA200501118A EA200501118A EA007146B1 EA 007146 B1 EA007146 B1 EA 007146B1 EA 200501118 A EA200501118 A EA 200501118A EA 200501118 A EA200501118 A EA 200501118A EA 007146 B1 EA007146 B1 EA 007146B1
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EA
Eurasian Patent Office
Prior art keywords
liquid media
data
organisms
biological organisms
characterized
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EA200501118A
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Russian (ru)
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EA200501118A1 (en
Inventor
Николай Андраникович Шахраманьян
Давид Георгиевич Хидашели
Георгий Леванович Сакварелидзе
Роберт Шалвович Вахтангишвили
Марина Николаевна Сошенко
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Давид Георгиевич Хидашели
Николай Андраникович Шахраманьян
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Priority to EA200501118A priority Critical patent/EA200501118A1/en
Publication of EA200501118A1 publication Critical patent/EA200501118A1/en
Publication of EA007146B1 publication Critical patent/EA007146B1/en

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Abstract

The invention relates to the field of biophysics, in particular to methods of analyzing liquid media in the composition of biological organisms in order to obtain initial information about the physical state of the latter as a whole. They are characterized by the fact that they create atlases of conformity of the states of biological organisms with the geometric, structural and color parameters of images of both shaped and non-shaped elements of liquid media of these organisms, analyze the dynamic and static characteristics of liquid media to stop the movement of the formed elements of liquid media, analyze the static characteristics of liquid media carried out after stopping the movement of the formed elements of liquid media, determine the degree and form of aggregation of the formed elements of liquid media, as well as the number of aggregated shaped elements form archived data, while, when the characteristics of shaped and non-shaped elements appear in the archived data that are different from those in the atlas data, all available information about this biological organism is entered into the archived data when the archived data are identical to those specified uniform and non-shaped elements of liquid media from different organisms in the amount of n, where n is any positive, an integer is greater than 2, transfer these characteristics of forms to the satin data elements of liquid media of organisms with those states of organisms set by them that matched the maximum number of times in a sample of accumulated archival data, making a preliminary conclusion on the basis of the comparison of atlas data and observation data on the state of the examined biological organism as a whole and on the possibility or impossibility the occurrence of certain types of pathologies in it, and based on a comparison of atlas data, observation data and archival data of the biological organisms being examined make a preliminary conclusion about the dynamics of changes in their states.

Description

The present invention relates to the field of biophysics, in particular to methods for analyzing liquid media as part of biological organisms in order to obtain initial information about the physical state of the latter as a whole. In this case, a biological organism in the present invention means a human, animal, insect organism, and a liquid medium or object of research refers to any fluid present in a given biological organism, such as blood, urine, saliva, lymphatic fluid, cerebrospinal fluid, semen, sweat fluid, bile, interstitial fluid, gastric juice, etc.

Prior art

The currently available technical solutions for analyzing the characteristics of liquid media of biological organisms mainly involve computerized systems for analyzing their biophysical parameters.

The known method and system for the analysis of blood cells of a biological organism (patent and δ 6330350, publ. 11.12.2001), allowing to determine the parameters of blood by the parameters of its nuclei and cytoplasm.

Also known is a system for visualizing and analyzing crystalline formations in the urine (patent 63δ 6391644, publ. 05/21/2002), which is the closest in technical essence to the claimed invention and includes a urine sample placement unit, an optical microscopy unit and a software-hardware complex for identifying . Analysis of crystalline formations in the urine provides information about the urological diseases of the biological organism.

A common disadvantage of all methods and systems for analyzing the characteristics of liquid media of biological organisms, including the ones listed above, is that they do not allow conclusions to be made about the physical state of the organism as a whole, but only individual organs, and do not provide primary information on the direction of further examinations identify or clarify certain pathologies of these organisms. In addition, these methods have not sufficiently high accuracy of the result of the analysis, which is caused by the neglect of statistical data and the lack of their constant replenishment in the process of obtaining the result.

Summary of Invention

The present invention is directed to the elimination of these disadvantages of currently available methods and systems for analyzing the liquid media of biological organisms and creating such a method and system that would allow:

a) receive express information about the physical condition of the organism as a whole;

b) receive primary information on trends (including pathological) development of the organism and the direction of further examinations;

c) increase the accuracy of the information produced in the process of accumulating data from surveys of liquid media of biological organisms.

This technical result is achieved by the fact that in the method of analyzing liquid media of biological organisms, including the selection of liquid media from biological organisms and the analysis of the formed elements of these media under a microscope, preliminarily create atlases of the correspondence of the states of biological organisms to geometrical, structural and color parameters of images of both shaped and elements of the liquid medium of these organisms, the analysis of the dynamic and static characteristics of liquid media is carried out to stop the movement of the shaped elements of liquid media, analysis of the static characteristics of liquid media is carried out after stopping the movement of the formed elements of liquid media, determine the degree and form of aggregation of the formed elements of liquid media, as well as the number of aggregated formed elements, form historical data, while the appearance of the formed and non-form elements, other than those in the satin data, enter into the archival data all the available information about a given biological organism, while accumulating in the archival data the iden The above specified characteristics of shaped and non-shaped elements of liquid media from different organisms in an amount n, where η is any positive, an integer is greater than 2, transfer these characteristics of shaped and non-shaped elements of liquid organisms of organisms with coincided the maximum number of times in a sample of accumulated archival data; on the basis of a comparison of atlas data and observation data, a preliminary conclusion is made about the state of the biological about the organism as a whole and about the possibility or impossibility of the occurrence of certain types of pathologies in it, and based on a comparison of atlas data, observation data and archival data of the biological organisms being examined, they make a preliminary conclusion about the dynamics of changes in their states.

Under the form elements are understood structural elements that determine the composition of the object as such, for example, red blood cells, white blood cells, platelets, etc. for blood; sperm, etc. for sperm, etc.

Unformed elements are understood to mean any elements that do not determine the composition of the object as such, for example, sugar crystals, cholesterol, uric acid, parasites, bacteria, viruses, etc. in blood, urine, etc.

- 1 007146

The method is implemented, for example, according to the flowchart shown in FIG. 1, as follows.

An object in the form of a sample of a fluid, such as blood, saliva, tears, urine, lymphatic fluid, mucus, etc., taken, for example, from a human, animal or insect, is placed in the object placement unit 1 (this can be a Goryaev camera and or simply the cover glass of the microscope stage). Using the microscopy unit 2, the image under study is enlarged to a predetermined size and transferred to the hardware-software complex 3, while the operator can observe an enlarged image of the object as on the information output unit 4, which is, for example, a computer display, a digital printer, a printer and t etc., as well as directly through the output optics of the microscopy unit, for example, through an eyepiece, binocular, stereoscope. Next, the operator manually or / and the computer program automatically selects the reference data stored in the memory of the atlas data block 5 for each fragment or set of image object fragments, while controlling the selection process through the output optics of the microscopy unit as well as through the information output unit. need corrects the specified process.

The computer program of the software-hardware complex 3 allows calculating as dynamic characteristics of the structural formations of an object, for example atoms, molecules (with a sufficient increase in the original image), associations of molecules, conglomerates, etc., such as their speed of movement, their acceleration and deceleration, time their movements and the moment of stopping, as well as the static characteristics of all structural formations in the image of an object, such as shape, color, linear dimensions and deviations of these characteristics from normal ones.

The accumulated statistical material allows to draw conclusions on the analysis of human blood, for example:

on a possible violation of lipid metabolism in terms of the ratio of the number of formed elements to non-shaped ones in the plasma;

on possible anemia on the structural and color characteristics of the formed elements in the plasma;

about a possible violation of the immune status of the movement of the formed elements in the plasma and their ability to aggregate;

on possible dysbacteriosis by the number of non-formed elements per unit volume of plasma; etc.

Comparing the actually measured data of the dynamic and / or static characteristics of the object with the reference ones from the block of atlases 5, where each state (pathology or tendency to development of pathology) of the biological organism is associated with a certain set of dynamic or / and static characteristics of the object, the operator manually or using the program makes a conclusion about the presence or tendency to the development or absence of a certain type of pathology, about the physical state of the organism as a whole and gives out targeted recommendations about the direction of further surveys. The obtained actual information is archived in the block of archival data 6 and in subsequent examinations of the same organism serves as the basis for concluding on the dynamics of the physical state of this organism.

Information from the archive data block 6 in the form of dynamic and / or static characteristics of the object enters the comparison block 7, information from the satin data block 5 also enters there. Block 7 compares the information coming to it from blocks 5 and 6, and if in the block of archival data 6 there is information that is missing in the block of atlas data 5, gives a command to the block of software and hardware complex 3 to request all available information on the biological organism under study.

From this point on, the dynamic and / or static characteristics of the same object (ie, blood, urine, saliva, lymphatic fluid, etc.) from other biological organisms with a similar request for all available information accumulate in the archive data block 6. . When accumulating in the block of archival data 6 such characteristics from η organisms, where n> 2 is a positive integer, the software of block 3 calculates in block 6 such a state of a biological organism from the requested information arrays, which is the maximum number of times found in these requested information arrays for the studied biological organisms. This information on the correspondence of the dynamic and / or static characteristics of the object to the above state of the biological organism from the archive data block through the comparison block 7 enters the satin data block 5.

Thus, in the course of the operation of the method for analyzing the characteristics of liquid media of biological organisms according to the claimed invention, the database of block 5 is constantly updated with statistical data, which increases both the range of identifiable pathologies or trends to their development and the accuracy of the information, that is, the accuracy of the result.

Wherein:

The object placement unit 1 is a type of device in which a sample of a fluid taken from a biological organism can be located and positioned so that this fluid sample is placed in the focus of the microscopes of the microscopy unit 2. In case, using infrared optical systems, the analysis biological organisms liquids

- 007146 is found directly inside their bodies, the object placement unit is a device consisting of fiber optic light guides, supplying radiation to a biological organism and receiving radiation reflected or transmitted through this biological organism;

microscopy unit 2 is a type of optical or electronic microscope device;

The block of software and hardware complex 3 is a kind of computer devices with software that implement the specified technical result of the invention;

the information output unit 4 is a type of information output device from the software / hardware complex 3 in the form of, for example, a monitor, a printer, a digital printing device, etc .;

the satin data block 5 is a memory cell of the software of the hardware-software complex 3, designed to store and activate satin data, that is, data on the correspondence of the states of biological organisms to the geometric, structural and color parameters of images of shaped and non-shaped elements of liquid media of these organisms;

the archive data unit 6 is a memory cell of the software of the software and hardware complex 3, designed to store and activate the actual data of the analysis of the characteristics of liquid media of biological organisms;

Comparison unit 7 is a computer device with software designed to compare information flows into it from the atlas data block 5 and the archive data block 6 and the comparison results to the software / hardware complex 3 and to the atlas data block 5.

The system for analyzing the characteristics of liquid media of biological objects contains functional connections between the blocks, while the object placement unit 1 is connected to the microscopy unit 2 by a mechanical connection, the microscopy unit 2 is connected to the software-hardware unit 3 by an optoelectronic communication unit, the software-hardware unit 3 is connected to the unit issuing information 4 by electronic communication, atlas data block 5, data archiving unit and comparison unit 7 are connected with electronic hardware complex 3, with a block a tlasna data 5 and the comparison unit 7 are interconnected by two-way electronic communication, the block of software and hardware complex 3 is connected with the archive data unit 6 by two-way electronic communication, and the archive data unit 6 is connected with the comparison unit 7 by electronic communication.

In the particular case of the implementation of the invention can be determined by the time of movements to stop the formed elements of the liquid media of biological organisms.

In the particular case of the implementation of the invention, the time of movement of the formed elements before they stop can be divided into time intervals, within which the characteristics of the formed elements of the liquid media of biological organisms are analyzed.

In the particular case of the implementation of the invention can be determined by the rate of deceleration of the movements of the formed elements of the liquid media of biological organisms.

In the particular case of the implementation of the invention, a microscope can be used with an increase sufficient for analyzing the cell structures of liquid media of biological organisms. This can be done both by electron microscopy and by other methods that allow one to obtain an increase in the analyzed image, sufficient to distinguish the cell structures of the object.

In the particular case of the implementation of the invention can be subjected to the analysis of three-dimensional images of the structures of the liquid media of biological organisms. This can be done, for example, by shooting a stereo pair of the analyzed object with the subsequent reconstruction of the three-dimensional image on the information output unit or by other methods.

In the particular case of the invention, a confocal optical system can be used and tomographic images of liquid media of biological organisms can be analyzed. This can be done, for example, by precisely moving the object placement unit in three directions (x, y, ζ) or by scanning the object in the same directions with layer-by-layer focusing of the microscopy unit on the object. Each layer-by-layer image is stored in the block of the software and hardware complex and is reproduced in the information output unit as separate sections of the object.

In the particular case of the invention, an infrared optical system can be used and liquid media inside the studied biological organisms can be subjected to analysis. Since biological objects, including the skin, muscle tissue, etc., have sufficiently high transparency in the near infrared region of the spectrum in the wavelength range of 0.7-1.2 μm (H. Gibson. Photographing in infrared rays. / Translation from English under the editorship of A. A. Petushkov. "Peace", Moscow: 1982, pp. 185-190), this can be done, for example, by irradiating a biological organism with optical radiation of a specified spectral range and recording the reflected or transmitted (depending on optical characteristics and thickness of the irradiated area of the biological organism) of the radiation carrying information about the liquid media of the biological organism with which this radiation has interacted.

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In the particular case of the implementation of the invention, the method can be environments of human organisms in a living or lethal state.

In the particular case of the implementation of the invention, the method can be used to be used for the analysis of liquid analysis of the liquid media of animal organisms in a living or lethal state.

In the particular case of the implementation of the invention, the method can be used for the analysis of liquid media of insect organisms in a live or lethal state.

In the particular case of the implementation of the invention, the analysis of liquid media of biological organisms and preliminary conclusions about the possibility or impossibility of the occurrence of certain types of pathologies of the biological organisms being examined and the dynamics of changes in the states of biological organisms can be carried out using software or / and without software. This can be done, for example, by creating software of the software-hardware complex 3, which automatically selects similar characteristics from the atlas data block 5 for the dynamic and static characteristics of uniform and non-shaped elements and displays information on the state of the biological organism to block 4, which corresponded to these characteristics of the object. The same selection can be carried out, for example, manually by the operator through a comparative analysis of the mentioned characteristics and the issuance of preliminary conclusions. It is also possible, for example, to combine automatic (with the help of software) and manual (without software) analysis of the mentioned characteristics and issue preliminary conclusions on the states of biological organisms.

In the particular case of the implementation of the invention, the analysis of liquid media of biological organisms and preliminary conclusions about the possibility or impossibility of the occurrence of certain types of pathologies of the biological organisms being examined and the dynamics of changes in the states of biological organisms can be carried out remotely from the place where the biological media are collected through telecommunications. This can be done, for example, by means of telemedicine systems, when the object placement unit and the microscopy unit are placed, for example, in the reception area, and all other units, for example, in the results processing area and issuing preliminary conclusions, while these areas can be separated in space as far as possible, and the connection between the above blocks from these two zones can be carried out by means of telecommunications, such as, for example, the Internet, radio communications, satellite communications, etc.

In the particular case of the implementation of the invention, the analyzes can be carried out before and after the substances are introduced into biological organisms, while preliminary conclusions about the possibility or impossibility of the occurrence of certain types of pathologies of the biological organisms being examined and the dynamics of changes in the states of biological organisms can be made by comparing the above analyzes. This can be accomplished, for example, by introducing into the biological organism orally or by injection, for example, liquids, such as water with dilute coral calcium, etc. This makes it possible to trace changes occurring in liquid media, such as blood, of a biological organism, for example, a person.

In the system for analyzing the characteristics of liquid media of biological organisms, including an object placement unit, a microscopy unit, a software and hardware complex, and an information visualization unit, this technical result is achieved by additionally creating an atlas unit, an archiving data unit and a observation data comparison unit in this system , satin data and archival data, while the block of the software and hardware complex is configured to store images from the object placement block in the states when there is no object on it and when an object is present on it, with the subsequent delivery of differential images to the information visualization unit.

Subtracting images from the object placement block as an image with an object minus an image without an object eliminates analysis errors associated with the initial presence of random particles, elements, microbes, etc. on the object placement block.

In the particular case of the implementation of the invention, the system can be configured to analyze the structures of the cells of the liquid media of biological organisms.

In the particular case of the implementation of the invention, the system can be configured to analyze the three-dimensional image of the structures of the liquid media of biological organisms.

In the particular case of the implementation of the invention, the system can be configured to analyze tomographic images of liquid media of biological organisms.

In the particular case of the implementation of the invention, the system can be made with the ability to analyze liquid media within the studied biological organisms.

In the particular case of the implementation of the invention, the system can be made with the possibility of remote analysis of liquid media of biological organisms.

In the particular case of the implementation of the invention, the system can be configured to issue an image or / and alphanumeric code. This can be done both by visualizing the images of the object, by visualizing some parameters of the object in the form of alphanumeric codes, and by visualizing the simultaneously mentioned images and alphanumeric codes. It may be

- 007146, for example, by simultaneous visualization of images of the formed and non-shaped elements of the blood and the results of the clinical or biochemical analysis of the same blood.

In the particular case of the implementation of the invention, the system can be configured to issue a three-dimensional image.

In the particular case of the implementation of the invention, the system can be made with the possibility of contactless fluid intake for analysis, for example using a laser perforator.

In the particular case of the implementation of the invention, the spectral characteristics of the transmittance of the lens and the sensitivity of the photodetector device can be made with the possibility of registering optical radiation in the range from ultraviolet to infrared regions of the spectrum.

In this case, the spectral characteristic of the transmission of the lens and the spectral characteristic of the sensitivity of the photodetector of the microscopy unit 2 allow you to record images of the object in the range from ultraviolet to infrared regions of the spectrum of optical radiation.

This allows you to record images of the object not only in the visible region of the spectrum, but also in the ultraviolet and infrared regions, which is important for the fluorescence of the object both in its natural state and during stimulation, for example, electromagnetic (including optical), electrical, mechanical, etc. d.

These signs are significant and interrelated with the formation of a stable set of essential features, sufficient to obtain the desired technical result.

Clinical examples

1. Patient S., 50 years old.

Complaints: abdominal pain, increased gas, skin rash, pallor of the skin, cyanosis under the eyes.

Blood tests according to the claimed method and system: uniform elements are aggregated into “coin columns” (FIG. 2), the time of movement of the uniform elements to their stop is an average of 6 minutes, a large number of non-uniform elements are present in the field of view.

Preliminary conclusion on the state of the organism as a whole on the basis of the study: anemia (comparing the structural and color characteristics of blood plasma elements with those from the satin data block), dysbacteriosis (comparing the number of unshaped elements in the blood plasma per unit volume with those from the satin data block) , impaired lipid metabolism (analyzing the ratio of formed elements with unformed elements in the blood plasma), impaired mineral metabolism (comparing the size and number of unformed elements blood plasma with those from the block of satin data), tissue hypoxia of the whole body (comparing the color indicators of formed elements in blood plasma with those of the block of satin data, taking into account the time of movement of the formed elements before they stop and the ability of the formed elements and non-shaped elements to aggregate) .

Recommended additional ultrasound examination of the abdominal cavity, complete blood count, biochemical blood test, spectral analysis of feces, urinalysis.

Results of recommended, classical studies:

complete blood count: the number of red blood cells - 3.2 x 10 12 / l, hemoglobin level - 98 g / l, white blood cell count - 4.3 x 10 8 / l, erythrocyte sedimentation rate - 20 mm / h;

biochemical blood analysis: cholesterol level - 8.2 mmol / l, lipid level - 3.06 mmol / l, triglyceride level - 3.53 mmol / l, quantitative uric acid content - 489.09 μmol / l, creatinine level - 133 , 0 mmol / l;

spectral analysis of feces: dysbacteriosis of 4 degrees;

urinalysis: specific gravity indicator - 1005, traces of proteins, a small amount of uric acid salts, the number of leukocytes - 3-5 units in the field of view;

ultrasound examination: the expansion of the cup-pelvis-like system of the left kidney, hepatosis, chronic cholecystitis.

Conclusion: the recommended, classical studies have confirmed the preliminary conclusion about the state of the organism as a whole, namely about the possible pathology of the patient in the abdominal organs, blood formation system, intestines, tissue hypoxia of the whole organism.

2. Patient G., 45 years old.

Complaints: persistent cough, nasal discharge, loss of appetite, abdominal pain, pallor of the skin.

Blood tests according to the claimed method and system: uniform elements are aggregated into "coin columns" (Fig. 2), the time of movement of the uniform elements to stop them is an average of 3 minutes, a large number of unformed elements are present in the field of view, a large number of poikilocytes (approximately 30 % of the total number of red blood cells) and echinocytes.

Preliminary conclusion on the state of the organism as a whole on the basis of the study: anemia (comparing the structural and color characteristics of blood plasma elements with those from the satin data block), dysbacteriosis (comparing the number of unshaped elements in the blood plasma per unit volume with those from the satin data block) , violation of mineral metabolism

- 5 007146 (comparing the size and number of non-shaped elements in blood plasma with those of the block of satin data), immunopathy (taking into account the time of movement of the formed elements before they stop and the ability of the formed elements and non-shaped elements to aggregate).

Recommended additional examination: complete blood count, spectral analysis of feces, urinalysis, the study of the immunological status.

Results of recommended, classical studies:

complete blood count: the number of red blood cells - 4.3 x 10 12 / l, hemoglobin level - 105 g / l, the number of leukocytes - 4.0 x 10 8 / l, erythrocyte sedimentation rate - 20 mm / h;

spectral analysis of feces: dysbacteriosis of 3 degrees;

urinalysis: the proportion indicator - 1002, the number of leukocytes - 1-3 units in the field of view;

immunological status: the number of lymphocytes - 1.6 x 10 8 / l, T-lymphocytes -50.2%, active T-cells - 25.7%.

Conclusion: the recommended, classical studies have confirmed the preliminary conclusion about the state of the organism as a whole, namely about the possible pathology in the patient from the side of the hemopoietic system, the intestines, and the immune system.

Brief Description of the Drawings

The essence of the present invention is illustrated in the drawing of the block diagram presented in FIG. 1, where are shown:

1- object placement unit;

2- microscopy unit;

- block software-hardware complex based on the computer;

4- information output unit;

- block of satin data;

- a block of archival data;

7- block comparison.

FIG. 2 depicts an example of aggregation of formed elements in the blood plasma in the form of “coin columns”.

Information confirming the possibility of carrying out the invention

The optimal embodiment of the invention is to create a software and hardware complex consisting of:

microscope BE1SL EMBA for transmitted light: Cp1ap lenses 10 times, 40 times, 50 times, 100 times; automatic lens change; 100 W halogen lamp; automatic adjustment of lighting; scanning steps along X - 0.3 microns, along - 0.3 microns, along Ζ - 0.015 microns; scanning speed up to 0.6 sq. / min; effective increase of the optical-digital path up to 1200 times;

color digital video camera Н1ТАСН1 3 ССЭ 1/3 inches; progressive scan; frame rate - 15 frames / s; the number of elements is 1.3 megapixels;

frame grabber ΜΑΤΚΌΧ ΜΕΤΕΟΚ.2 / ΕΙΝΚ;

computer P 4, 19 inch monitor; operating system №ΙΝΏΟ№8 XP.

The present invention is industrially applicable, since it is not based on the use of new hardware not manufactured by industry, which ensures the realization of the functions of analyzing the characteristics of liquid media of biological organisms, but on a new combination of connections between these realizations.

Claims (24)

1. A method for analyzing the characteristics of the liquid media of biological organisms, including the selection of liquid media from biological organisms and the analysis of the shaped elements of these media under a microscope, characterized in that atlases corresponding to the states of biological organisms are preliminarily created with the geometric, structural and color parameters of images of both shaped and non-shaped elements of the liquid media of these organisms, the analysis of the dynamic and static characteristics of liquid media is carried out until the movement of the formed liquid elements is stopped Ed, the analysis of the static characteristics of liquid media is carried out after stopping the movement of the formed elements of the liquid medium, the degree and form of aggregation of the formed elements of the liquid medium, as well as the number of aggregated shaped elements are determined, archive data is generated, and, when the characteristics of the formed and non-shaped elements appear in the archive data that are different from those in the satin data, all available information about the given biological organism is entered into the archive data, when identical data indicated x are accumulated in the archive data the characteristics of the formed and non-shaped elements of liquid media from different organisms in the amount of n, where n is any positive integer greater than 2, these characteristics of the formed and non-shaped elements of the liquid media of organisms with the corresponding maximum conditions of organisms that correspond to the maximum the number of times in a sample of accumulated archive data, based on a comparison of satin data and
- 6 007146 observation data make a preliminary conclusion about the state of the examined biological organism as a whole and about the possibility or impossibility of certain types of pathologies in it, and based on a comparison of satin data, observation data and archival data of the examined biological organisms, make a preliminary conclusion about the dynamics of changes in their conditions .
2. The method according to claim 1, characterized in that determine the time of movement to stop the formed elements of the liquid media of biological organisms.
3. The method according to claim 2, characterized in that the movement time of the shaped elements before they stop is divided into time intervals within which the characteristics of the shaped elements of the liquid media of biological organisms are analyzed.
4. The method according to claim 1, characterized in that they determine the speed of slowing down the movements of the shaped elements of the liquid media of biological organisms.
5. The method according to any one of claims 1 to 4, characterized in that a microscope is used with an increase sufficient to analyze the cell structures of liquid media of biological organisms.
6. The method according to claim 5, characterized in that the analysis is subjected to three-dimensional images of the structures of liquid media of biological organisms.
7. The method according to claim 6, characterized in that they use a confocal optical system and analyze tomographic images of liquid media of biological organisms.
8. The method according to claim 7, characterized in that they use an infrared optical system and analyze the liquid environment inside the studied biological organisms.
9. The method according to claim 8, characterized in that the method is used to analyze the liquid media of human organisms in a living or lethal state.
10. The method according to p. 8, characterized in that the method is used to analyze the liquid media of animal organisms in a living or lethal state.
11. The method according to claim 8, characterized in that the method is used to analyze the liquid media of insect organisms in a living or lethal state.
12. The method according to any one of claims 9 to 11, characterized in that the analysis of the liquid media of biological organisms and preliminary conclusions about the possibility or impossibility of certain types of pathologies of the examined biological organisms and the dynamics of changes in the conditions of biological organisms are carried out using software and / or without software.
13. The method according to p. 12, characterized in that the analysis of the liquid media of biological organisms and preliminary conclusions about the possibility or impossibility of the occurrence of certain types of pathologies of the examined biological organisms and the dynamics of changes in the conditions of biological organisms are made at a remote access from the place of intake of liquid media of biological organisms through telecommunication means of communication.
14. The method according to p. 13, characterized in that the analyzes are carried out before and after the introduction of substances into biological organisms, while preliminary conclusions about the possibility or impossibility of certain types of pathologies of the examined biological organisms and the dynamics of changes in the conditions of biological organisms are made based on a comparison of the above analyzes.
15. A system for analyzing the characteristics of the liquid media of biological organisms, including an object placement unit, a microscopy unit, a hardware-software complex unit, and an information visualization unit, characterized in that they additionally create an atlas unit, a data archiving unit, and a comparison unit for observation data, satin data and archive data data, while the object placement block is connected to the microscopy block by mechanical communication, the microscopy block is connected to the block of the hardware-software complex by optoelectronic communication, the program block of the hardware-software complex is connected to the electronic information output unit, the satin data unit, the data archiving unit and the comparison unit are connected to the electronic-hardware complex software unit, while the satin data unit and the comparison unit are interconnected by two-way electronic communication, the software the hardware complex is connected to the archive data block by two-way electronic communication, and the archive data block is connected to the electronic communication comparison block, while the hardware-software complex block nen to store the images from the object placing unit in a state where it is no object and object when it is present subsequent to the issuance of the differential image in the block information visualization.
16. The system of clause 15, wherein the system is configured to analyze cell structures of liquid media of biological organisms.
17. The system according to any one of paragraphs.15-16, characterized in that the system is configured to analyze a three-dimensional image of the structures of liquid media of biological organisms.
18. The system according to 17, characterized in that the system is configured to analyze tomographic images of liquid media of biological organisms.
19. The system according to p. 18, characterized in that the system is configured to analyze liquid media inside the studied biological organisms.
20. The system according to claim 19, characterized in that the system is configured to remotely analyze liquid media of biological organisms.
- 7 007146
21. The system according to claim 20, characterized in that the system is configured to issue an image or / and alphanumeric code.
22. The system according to item 21, wherein the system is configured to issue a three-dimensional image.
23. The system according to item 22, wherein the system is configured to contactlessly take the fluid for analysis, for example using a laser punch.
24. The system according to item 23, wherein the spectral characteristics of the transmittance of the lens and the sensitivity of the photodetector system are made with the possibility of recording optical radiation in the range from ultraviolet to infrared regions of the spectrum.
EA200501118A 2005-07-13 2005-07-13 Method and system for analysis of the characteristics of liquid media of biological organisms EA200501118A1 (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
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WO2014127285A1 (en) * 2013-02-18 2014-08-21 Theranos, Inc. Systems and methods for collecting and transmitting assay results
WO2015039039A1 (en) * 2013-09-13 2015-03-19 Theranos, Inc. Methods, devices, and systems having multiple passwords
US9182388B2 (en) 2005-05-09 2015-11-10 Theranos, Inc. Calibration of fluidic devices
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9858660B2 (en) 2011-09-25 2018-01-02 Theranos, Inc. Systems and methods for collecting and transmitting assay results

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9182388B2 (en) 2005-05-09 2015-11-10 Theranos, Inc. Calibration of fluidic devices
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9858660B2 (en) 2011-09-25 2018-01-02 Theranos, Inc. Systems and methods for collecting and transmitting assay results
WO2014127285A1 (en) * 2013-02-18 2014-08-21 Theranos, Inc. Systems and methods for collecting and transmitting assay results
CN105164508A (en) * 2013-02-18 2015-12-16 赛拉诺斯股份有限公司 Systems and methods for collecting and transmitting assay results
CN105164508B (en) * 2013-02-18 2019-04-02 赛拉诺斯知识产权有限责任公司 System and method for acquiring and transmitting measurement result
WO2015039039A1 (en) * 2013-09-13 2015-03-19 Theranos, Inc. Methods, devices, and systems having multiple passwords

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