EA002807B1 - Agticultural compositions containing bacteria - Google Patents

Abstract

1. A composition for enhancing the activity of an agriculturally effective active ingredient comprising: (a) an agriculturally effective active ingredient selected from the group consisting of a plant growth regulating agent, herbicide, systemic fungicide, and a systemic insecticide; and b) an enhancer for enhancing the activity of said active ingredient containing spores, cultures, or suspensions of a suitable Bacillus or soil bacteria at a pH sufficiently less than 7 to maintain said enhancer in spore form, provided that the composition does not contain plant growth hormones when said plant growth regulating agent is a plant growth stunting agent. 2. The composition of Claim 1 wherein said plant growth regulating agent comprises growth stunting agents, growth improving agents, and herbicides. 3. The composition of Claim 1 wherein said plant growth improving agent comprises plant growth hormones, such as gibberellins, cytokines, auxines, sodium ortho-nitrophenolate, sodium para-nitrophenolate, sodium 5-nitro-guaicolate, polyhydroxycarboxylic acids of 2, 4, 5, and 6 carbon structures, ethephon, nitrogen- or phosphoro-containing fertilizer. 4. The composition of Claim 2 wherein said plant growth stunting agent comprises chlormequat chloride, meliquat chloride, maleic hydrazide and esters thereof. 5. The method of Claim 1, wherein said plant growth regulating agent comprises a herbicide, mepiquat chloride, chlorinquat chloride, or ethephon. 6. The composition of Claim 1 wherein said enhancer contains a strain of bacteria from the genus Bacillus. 7. The composition of Claim 1 wherein said enhancer contains a B. cereus or B. megaterium. 8. The composition of Claim 1 wherein said enhancer contains ATCC 55675. 9. A nonliquid composition of Claim 1, comprising: (a) an agriculturally effective active ingredient, selected from the group consisting of a plant growth stunting agent, ethephon, a plant growth hormone, naphthalenacetic acid, sodium ortho-nitrophenolate, sodium para-nitrophenolate, sodium 5-nitro-guaicolate, polyhydroxycarboxylic acids, and a herbicide; and (b) an enhancer for enhancing the activity of said ingredient containing spores or cultures of a suitable Bacillus or soil bacteria, provided that the composition does not contain plant growth hormones when a plant growth stunting agent is the active ingredient. 10. The composition of Claim 9 wherein said active ingredient is mepiquat chloride, chlormequat chloride, or ethephon, glyphosate, or sulfosate. 11. A method for increasing the number of fruiting sites on a fruiting plant by (i) diluting a composition containing (a) an agriculturally effective active ingredient of a plant growth regulating agent; and (b) an enhancer containing spores of a suitable Bacillus or soil bacteria at a pH of less than 7 and sufficiently low to maintain said Bacillus in spore form, and (ii) applying the diluted composition to foliage of a plant containing fruiting sites. 12. The method of Claim 11 wherein said fruiting plant is cotton. 13. The method of Claim 11 wherein said plant growth regulating agent is a plant growth stunting agent or ethephon. 14. The method of Claim 11 wherein said enhancer contains a strain of bacteria from the genus Bacillus. 15. The method of Claim 11 wherein said enhancer contains a B. subtilis, B. megaterium, or B. cerew. 16. The method of Claim 11 wherein said enhancer contains ATCC 55675. 17. A method for enhancing the effectiveness of an agriculturally effective active ingredient by applying to a plant (a) an agriculturally effective active ingredient of a plant growth stunting agent, ethephon, or a herbicide; and (b) an enhancer containing spores, cultures, or suspensions of a suitable bacillus or soil bacteria. 18. The method according to Claim 17 wherein said agriculturally effective active ingredient is a triazine, glyphosate, or sulfosate, mepiquat chloride or chlormequat chloride. 19. The method of Claim 17 wherein said enhancer contains a strain of bacteria from the genus Bacillus. 20. The method of Claim 17 wherein said enhancer contains B. cerew or B. megaterium. 21. The method of Claim 17 wherein said enhancer contains ATCC 55675. 22. A composition of Claim 1, comprising a Bacillus in spore, culture or suspension form and a plant growth stunting agent, wherein the composition does not contain ATCC 55675. 23. The composition of Claim 22 wherein said active ingredient is mepiquat chloride. 24. The composition of Claim 23 wherein said bacteria from the genus Bacillus are B. cereus, B. subtilis or B. megaterium.

Description

The invention relates to the treatment of plants with a composition containing an agriculturally useful active ingredient and a reinforcing (improving) additive containing a substantially pure culture of bacteria selected from the genus BaeShik or soil bacteria. The culture added may be in the form of cells, spores or suspensions.

Background of the invention

Producers of agricultural fertilizers are always looking for ways to enhance (improve) the effectiveness of the active ingredients used in the processing of plants. In particular, this is correct when the material used is a plant growth regulator (slowing or enhancing growth), a herbicide, or a systemic agent (for example, an insecticide or fungicide). Transport processes in a plant and movement between different plant tissues are very important and, in some cases, can be the primary factor determining the effectiveness of the applied ingredient. For some active ingredients, an improvement in the transport mechanism may lead to an increase in action with existing application rates, the need for a less active ingredient, or the ability to process new species that were previously resistant to the active ingredient.

Mepiquat chloride is an active ingredient, for the effectiveness of which important are the processes of its absorption by plants and transport in them. Mepiquat chloride (Ν, Ν-dimethylpiperidinium chloride) is used annually as an active ingredient to slow the vegetative growth of cotton plants and increase the harvest time on millions of acres of cotton. Mepiquat chloride can also be used to some extent to treat potatoes, sweet potatoes, peanuts, grapes, cereals, wheat, citrus fruits, tomatoes, and onions.

Mepiquat chloride has the effect of slowing vegetative growth on cotton plants, thus forcing the plant to direct its energy to the development of fruits (cotton seed pods). With the appropriate use of mepiquat chloride for the treatment of plants that are beginning to show excessive vegetative growth, the yield of a cotton plant can be maintained or increased without harming the plant. The effects of growth retardation are particularly desirable when the crop is grown in fertile soil or after the occurrence of weather conditions that are more favorable primarily for plant growth, and not for the development of its fruiting.

Cotton plants have a predictable life cycle and growth period. The emergence of cotton seedlings occurs on days 7-10 after sowing seeds in the ground. In a cotton plant, a growth of the root system is observed, as well as an increase in the height of the plant as a result of the growth of the stem and shoots due to the structure related to vegetative growth until about 4-8 knots appear. After this, the plant produces a reproductive shoot (1st fructiferous plot), and all subsequent shoots are reproductive. The cotton workers are trying to regulate plant growth to ensure that the ratio of vegetative growth to reproductive growth (the emergence of the seed box), which will be favorable for obtaining the desired limits of reproductive growth.

Cotton heads usually prefer to see about 2 inches (5 cm) between the nodes of the main stem. This ratio represents a balance between too strong reproductive growth (the appearance of a seed box), which can cause the development of a plant that is ahead of the speed of vegetative growth and the restriction of such growth before the crop becomes maximum, and too strong vegetative growth that reduces the number of mature seed boxes.

Cotton plants, which have directed most of the plant's beneficial energy to vegetative growth, are called highly branched cotton and produce smaller seed pods that ripen later and are vulnerable to extreme weather conditions for longer periods of time. Cotton, which shows signs of strong branching, is easy to see from abnormal plant growth relative to the number of seed pods and the number of reproducing nodes of the main stem. Mepiquat chloride is used to prevent strong branching of cotton, which is achieved by changing the characteristics of plant growth.

The lateral shoots of the main stem are usually always located alternating from different sides of the stem. Each branch location of the shoot is called a knot, with 5-7 knots forming from the leaves of the cotyledon to the first fruiting branch with true leaves. Counting nodes is from the bottom of the plant to the top of the main stem. The length of the internode is the distance between the branches of the shoots, and a new node is formed approximately every three days. For measurement and comparison, the number of nodes and the length of interstices after 8 nodes are usually used; this allows leveling variations in fruit-bearing nodes between different plants, since it can be said with confidence that fruit-bearing shoots will be formed by the 8th node. Thus, it is accepted that the counting of fruit bearing nodes starts from the first reproductive node, usually No. 7 or No. 8.

The locations of the fruits of cotton are called plots. Each fruiting shoot will form 1-6, normally about 3, locations of fruits (plots) with the formation of boxes on each shoot approximately every six days. The stage of the formation of new sites and the beginning of reproductive growth in cotton plants is called the stage of the pinhead areas because of their barely noticeable size. After about 510 days, the pad grows to a size similar to that of a match head; this period in the life cycle of a plant is called the stage match head stage. The stage of the match head of the pad continues to grow until the fruit reaches a size approximately equal to the size of the nail of the middle finger of the hand, before it blooms (early flowering). Three days later a seed box will form under the flower. Approximately thirty days after early flowering, the seed pods of the fruit fully ripen and are ready for harvest. About 80% of the total cotton crop ripens within 3 weeks after early flowering and 95% of the total crop matures within 5 weeks after early flowering.

Typically, mepiquat chloride is used to treat cotton plants in one of two ways. The method, which was used until about 1986, included a single treatment with 8–16 ounces of a 4.2 wt.% Solution per acre during early flowering. This type of single treatment did not regulate plant height, despite the fact that, in particular, a decrease in the yield of cotton was sometimes observed if the plants were exposed to a damaging factor during or after treatment.

Since 1987, there has been a tendency to use mepiquat chloride as a series of treatments, each of which contains doses (of this compound) lower than those used in a single treatment (standard dose). The first treatment is carried out at the stage of the match head of the platform, after which after 714 days, subsequent processing is carried out. Both treatments are subject to rates ranging from approximately 0–8 ounces to a 4.2 wt.% Solution per acre with a specific application of the rates depending on whether the cotton plant showed signs indicating that it was exposed to damaging factors. (no treatment), signs of moderate growth (approximately 2 ounces of solution per acre) or strong growth (approximately 4 ounces of solution per acre). After this, two additional treatments can be followed at intervals of 7-14 days with application rates that can be increased to approximately 8 ounces of a 4.2 wt.% Solution of mepiquat chloride with specific application rates depending on the number of cases of vegetative growth on the field. Conducting additional experiments by individual cotton growers has led to the emergence of a wide variety of multiple application rates.

It would be desirable if the use of mepiquat chloride could be integrated into the processing system, which would increase the mass of plant tissue in the roots, stems and leaves in order to ensure a higher level of nutrient metabolism, despite the fact that at the same time restriction of vegetative growth is provided to enhance the production of fruits.

When developing methods of treating plants with herbicides, it is always advisable to increase the strength of their action without a corresponding increase in application rates. In many cases, treatment with herbicides could also maintain the activity of herbicides without increasing their number. Farmers and herbicide producers are faced with the task of suppressing the number of weedy and harmful plants without excessive use of the tested herbicides, if such regulation can be carried out at all. Some plants, such as E1opba Riklei, Vi11 Otakk, Vetshiba Otakk, Eod Eeppe1 and Rpshtok, are all too highly resistant to herbicides for these active ingredients to be quite effective.

It would be useful to have a means to increase the potency of active agricultural agents, such as herbicides, without increasing the amount of active ingredients used.

Summary of Invention

The object of this invention is to provide a composition and a method for its subsequent use in order to increase the strength of the effect of agriculturally effective active ingredients.

The next object of the invention is the composition and method for its use on fruit-bearing plants and seeds, which increases the number of fruit-bearing areas on the treated plants in order to ensure an increase in the yield of fruits.

Another object of the invention is a composition and method for its use, in which the treated plants grow under more healthy conditions.

In accordance with these and other objects of the invention, which will become apparent from this description, the composition according to the invention includes:

(a) an agriculturally effective active ingredient and (b) an improving (reinforcing) additive, including a culture that contains bacteria of the genus VasChik or soil bacteria in the form of cells, cultures or suspensions and in sufficient quantity to increase the effectiveness of the specified active ingredient . Preferably, the enhancer does not contain plant growth hormones when used in combination with plant growth retarding agents, such as mepiquat chloride, since the effect of plant growth hormones on suppressed plants is suppressed.

The compositions according to the invention increase the potency of the active agricultural ingredients used. The same amount of active substance that is usually used will be more effective. Lower concentrations of the active substance can be used to achieve the same effect that is achieved as a result of the application of higher generally accepted application rates. Additionally, plants that have been treated with compositions in accordance with the invention are healthier with the benefit that, being more resistant to diseases or other damaging factors, have a large number of fruit bearing areas and increased yield.

Detailed description

The invention provides a method of treating plants with a composition containing an effective active agricultural ingredient and improving (enhancing) additive containing a culture of bacteria selected from the genus VasChik or soil bacteria in an amount sufficient to increase the effectiveness of the active effective agricultural ingredient applied simultaneously, before or after applying the reinforcing (improving) additives. The increase in efficacy attributable to the component of the improving additive containing bacteria can be used to reduce the amount of effective active agricultural ingredient used or, in the case when the active ingredient is used at the same rate, then the bacterium bacteria increase the effectiveness of the effective active agricultural ingredient used. Such an increase in efficacy is useful in suppressing the amount of weeds, which, otherwise, when applying regular herbicide treatments is difficult.

Containing bacteria, reinforcing additive

The component of the improving, reinforcing additive contains spores, cultures and suspensions of bacteria of the genus VasShik or soil bacteria. Preferably, the bacteria for the enhancement component is in the form of spores, which is the result of suitable regulation of temperature, pH, salinity, etc.

Bacteria suitable for use in the present invention include those bacteria that exhibit the ability to increase the effectiveness of an agriculturally effective effective ingredient through any mechanism. Methods of screening bacterial strains for their biological activity and, therefore, their ability to enhance the effectiveness of plant growth regulators, herbicides, systemic fungicides or systemic insecticides will become obvious to ordinary specialists in the field and will be disclosed later in this document, and further supported examples. For example, one of the bacterial candidate strains, such as VaeShik segeik, can be grown and stored under standard laboratory conditions. (See, for example, Bigwood! A1. Musical Group: A Lactus! Ogu Mapia1 So1b 8rppd Nagyog Luthog Ogregkk (1989)). Specific plants can be selected for their susceptibility or resistance to a specific agriculturally effective compound, such as a herbicide or plant growth regulator. For example, as presented later in the examples section here, cotton can be used to evaluate the biological activity of bacterial strains in combination with plant growth regulators; This assessment is done by counting the number of fruit bearing areas and / or the number of produced seed bolls, and comparing them with the number of fruit bearing areas and / or the number of produced seed bolls of plants treated in the same way, but using only bacteria or plant growth regulator. Alternatively, in order to assess the ability of bacterial species to increase fungicide activity, in order to monitor plant diseases, plants that are sensitive to certain fungal diseases, such as the disease (rot) of tomato plant leaves (1 1 1аа £ Ьщщ1И). potato scabs (ro! a! o xab), wheat stalk rust (\\\ '11ea1! tick tick!), soot grain breads (sogt kti!) or leaf rust (1е £ geek!) of various plant species can be grown in controlled conditions and processed either before, or during, or after sowing with the appropriate fungicide, or fungicide mixed with a bacterial candidate species or only with a bacterial species without admixture of a fungicide. These types of experiments can be carried out in the fields under partially controlled conditions, such as greenhouses, or under relatively controlled conditions, such as conditions created in incubators (doo \\ E1t lbatc). These experiments include the usual procedure for screening organisms for their enhancement effect along with substances or compositions on known plant species, the parameters used to evaluate the effects are also known and measured in the usual way.

These similar types of screening can be applied with the bacterial strains described herein in order to provide the effect of enhancing specific agriculturally effective active ingredients, i.e. deposited at Atepsap Tour Sy Inge Soynessyop as an ATCC 55675 Vaschiz segeis strain provides an enhancement effect for plant growth regulator mepiquat chloride and atrazine herbicide; in addition, they can be used to screen for bacterial species in order to provide possible enhancement to other effective active agricultural ingredients, such as systemic insecticides and systemic fungicides.

Particularly preferred are cultures, spores, or suspensions of soil bacteria spread between the roots or among the roots of the growing overgrowth of plant types growing in the soil spread in the area. The use of soil bacteria common in the area to treat leaves using application rates in the range of approximately 0.1 x 10 ¹⁰ CFU / acre to 10 x 10 ¹⁰ CFU / acre enhances the natural mechanisms associated with growth and reproduction to sufficient the level at which the active agricultural ingredient exhibits increased activity on or within the treated plant.

A wide variety of bacterial species within the genus Vaschiz and among known species of soil bacteria is acceptable for the present invention. The following list is a list of species suitable for the present invention.

Bacteria of the genus Vaschiz

VasShiz IskisMapiz

VasShiz asSho! Himgoz1pz

VasShiz a1s1orkIz

WasShiz αίνεϊ

VasShiz Atto & IsozIiz

VasSiz Attowapz

WasShiz Atu! ONDIE / Asleepz

WasShiz atu! O1uNziz

WasShiz upshgasgs

WasShiz apeigSho1uVsiz

WasShiz argapiz

WasShiz αζοίοβχαηζ

Vaschiz Bgeu ^ s

VasShiz Vasiiz

VasShiz sarNouaIz

VasSiz Segeiz

VasShiz S1GSI!

VasShizygo ^ a ^ eIoziz

WasShiz soa% and1app

WASSHIZ SO1 / OI <1 of

You Shiz Suslocker (ap1siz

VasShiz er1p1gu1iz

WASShIZ / AZNZHOZIZ

WasShiz £ Isotisiz

WashSchiz / icpiz

Washschiz / Gisera

WasShiz β-isUziz

WasShiz ξΐούϊξϋ

WAS SHOW% 1

WasShiz TzoShiz

WASSHIZ 1AEUO1ASIZIZ

WasShiz 1aguai

VasShiz 1Seurozrogiz

WasShiz 1aShiz

WasShiz 1epItogiz

WasShiz

VasShiz Isket / Ogtgz

VasShiz Tasegapz

VasShiz tasdiappes ^ s

VasSiz Tagosapiz

VasShiz TasgoShez

Vasshiz tes1iza

WashSchiz those & a1g1it

WasShiz tusoShez

WasShiz ηαϋο

WasShiz ηΐξήβοαης

Vasshiz rain

VasShiz Raarsiz

VasShiz raShoShePIziz

Vasziz ragag> pz

Wasziz Razleit

VasSiz

VasShiz rorinae

WASSHIZ RIY / a

WasShiz Riy / Ayepz

VasShiz RitIiz

WasShiz & Scotland

VasShiz rzuskgorkIiz

Wasziz zuskgosskaggo! Usis

VasShiz Gaset IasIziz

Vaschiz vrkaepsiz

WasShiz 8 {eGo1kegtorkNiz

VasShiz ZyiPz

WasShiz 1 kegtoAet1g1rsapz

VasShiz 1k1at1po1uIziz

WasShiz Shigt & eppzgz

WasShiz IShRA§e11aShz

WasShiz wa! Shiz

Soil bacteria

Ascgotine RezN / Er

A1СН ^ epez ei1gorkiz

A! SaI% ezez 1a1iz

Atuso1a! And aiShgorYsa Agskagtsrit% erkuga Apkgoaaeg ugzsoziz Ago1oas1eg chroococcum Bacillus asShouogapz Bacillus geugz Bacillus cereus Bacillus sggsiNapz Bacillus tzoShiz Bacillus 1a1egozrogiz Bacillus those% a1eNit Bacillus τηο] ανβη3Ϊ3 Bacillus mycoides Bacillus raz1eigi Bacillus ro1utuha Bacillus rzuskgozasskago! UNsiz Bacillus zrkaepsiz Bacillus subtilis Bacillus u1zsoziz Sky ku ^ goaSgosuapea S1oz1psNit akzopit Susa (oas1eg / fuscus SuYurka ^ a] okpzopae Epz1 / ez aVkaegepz NurkopisgoYit / asShz M1sgosossiz 1i1eiz Mitotopozroga ska1sea Mgsgotopozroga soegi1ea Mgsgotopozroga §ggzea

Mgsgotopozroga ro1u (go1a M1sgo1e1gazroga% 1aisa Musoaaepit αξτί Musoaaegsht ayMepze Musoas1egsht aigit Musoaaegsht skNae Musoas1eg1it skiiepze Musoaaegsht lattice / err Musoaaegsht / Opio Musoas1egsht peoaigit Musoaaegsht räga / OPIK Musoaaegsht 1eggae Musoas1epit ShegtogezgzPIe Muhosossiz sogaPoShez Muhosossiz βιΙνα5 Muhosossiz tasgozrogiz Muhosossiz hatkiz MapposuzIzeheVepz №1gozo1oiz Tisch / ogtgz Νοοαπϋα gazShepz з WisagShoz a1biz Oskgobaagit ashkgorg Ro1uapgsht s11i1ozit RzeiVotopaz azevrpoza RzeiVotopaz $ ioghessepz RzeiAotopaz% 1-1 ke1 KakpeIa atsiaNNz Zasskagoaaaogsht asittaSht ZasskagotopozrogaSH

ZeggaIa Tagseseps

81ger1otusez αηαηάϋ

81ger [by aigeo / asgepz

81grrrrrrrrrrrrrrrrrrrrrr skag / geizu

81ger (supeiz

8 (hercuses sutagoppeza

8 & tusus <Laz (a1cggotspez

81ger1us βотνοξήαβυ ^

81 $ 1 $% e1aIsiz

81gerGotuse.ch ku ^ gossorgsiz

81ger1otusez t (%% of

8 (gerUtusez kajgae

81ger1otusez IrtapN

8 (Gertustez ct & zragiz $ 1ger1otuse8 tazzazrogeiz

81gr

81g (ο οΓΪ / er

81ger1otusez opuaepz ^ s

81girls rapt1iz

81ger1otusez rkaeoskgoto epez 8 ^ (^ ger1otusez rzei0o g1zeo1iz 81geryutusez roseum $ auiz

8 & er {by means of gypsumpozkekükm

81gr (given gig ^ erzepzgz

81grutuzezssego ^ gapi! A1iz

8 (HerUtusy 1ohu1g1st

81grrrrrrrrrrrrrrrrrrrrrr

81ger1otusez? 11aseise

8 (gerutuzes νϊοΐαήιιζ TkegtoasNpootuzez ui1% apz

Tukusasiz skzkapsaps

Tukaschiz (kyragiz not fluorescent Rzeiskhpopaz

Jungoit

A% gos1egsht

Sogupeaaegsht Igea / aaepz

Apkgoa & er igea / aaepz

Reze ^ otopaz aegidtoza

WashSiz / aze <1 of

Гεηΐήβοαηχ

Muscle Beer PYe1

Aegoastedededepez

Wizapit TopSH / Ogte

Shz1or1azta sarszykya

RetsShtit skguzo ^ epis

Particularly useful are the species of B. g. 11118, V. segeiz and V. Medailegsh, VasShiz zyly1Ϊ8 and V. segeiz are naturally propagating saprophytes of soil, which are found everywhere in the world. Published in

The 1992 supplement to Leshepsup Tour SJiigi Sojes1yup lists 182 different species of B. zy1yz (listed here as links). The list below is a list of species B. ziyshz that can be used in the present invention.

D ZiMNz

ATSS 10783

ATCC 15818

ATCC 15819

ATSS 15245 (VasShiz payo)

ATSS 15134 (VasShiz ishYaaPaShz)

ATSS 13542

ATCC 13472

ATCC 15575

ATCC 27505

ATSS 21697 (Acbottocaster P11p1os1az1e8)

ATCC 15811

ATSS 4925 (VasShiz pfgShapsaps)

ATSS 27370

ATCC 6051a

ATCC 7003

ATCC 11838

ATCC 15563

ATCC 33234

ATCC 25369

ATSS 27689

ATCC 55033

ATCC 13933

ATCC 15244

ATCC 27328

ATCC 12695

ATCC 12100

ATCC 21554

ATCC 21555

ATCC 15561

ATCC 15562

ATSS 9799

ATSS 12711

ATSS 13407

ATCC 7067

ATSS 29056

ATCC 31524

ATCC 31526

ATCC 21359

ATCC 21360

ATCC 13954

ATCC 13955

ATCC 15044

ATCC 33677

ATCC 31003

ATCC 31522

ATCC 465 Ν.

ATCC 12432

ATCC 43223

ATCC 13952

ATCC 13953

ATCC 14662

ATCC 15039

ATCC 15040

ATCC 15041

ATCC 15042

ATCC 15043

ATCC 15181

ATCC 15182

ATCC 15183

ATCC 15184

ATCC21183

ATCC 21336

ATCC 49343

ATCC 6537

ATCC 21394

ATSS 8473

ATCC 31523

ATCC 31525

ATCC 31527

ATCC 29233

ATCC 14660

ATSS 14661

ATCC 31268

ATCC 4925

ATCC 55405

ATCC 9524

ATCC 15476

ATCC 23858

ATCC 23859

ATSS 7060

ATCC 7058

ATSS 7059

ATSS 7480 (VasShiz epporagazyhziz).

ATCC 21584

ATCC 31022

ATCC 21331

ATCC 21332

ATCC 21777

ATCC 21778

ATCC 6598 (Vaschiz Psienpus)

ATCC 49822

ATCC 23857

ATSS 19221

ATCC 9858

ATCC 21742

ATCC 4529

ATCC 35148

ATCC 33608

ATCC 19549

ATCC 19550.

ATCC 21556

ATCC 31340

ATCC 49760

ATCC 53325

ATSS 14807

ATCC 21228

ATCC 15512

ATCC 15841

ATCC 10774

ATCC 31091

ATCC 31092

ATCC 31094

ATCC 31096

ATCC 31097

ATCC 35946

ATCC 39374

ATCC 11774

ATCC 15116

ATCC 35021

ATCC 31954

ATSS 19062

ATCC 23059

ATCC 53115

ATSS 15115

ATCC 13956

ATCC 21952

ATSS 82

ATCC 21603

ATCC 31785

ATCC 21697

ATCC 15477

ATCC 31098

ATSS 19162

ATCC 14617

ATCC 14618

ATCC 33713

ATCC 33714

ATCC 55422

ATCC 6461 ATCC 21007

ATSS 21770

ATCC 6984

ATSS 19163

ATCC 21663

ATSS 19217

ATSS 19219

ATSS 19220

ATCC 21005 ATCC 21006

Preferred for use in the present invention, the strain of B. ziShz includes TRIPS. Initially, V. zyyshz SPOZ was recognized as a biological fungicide and was commercially used for seed treatment under the name ΚΟΌΙΑΚ ™ HB or Θϋδ 2000 ™ Oi1a £ zop, 1ps., P1apo, Tehaz 75093 (EPA Ced. No. 7501-146). This product is suitable in the form of a 2.75% powder containing at least 5.5 x 10 ¹⁰ viable spores per gram and should be applied in the range of 2-4 ounces per 100 pounds of seed. Taking into account the instructions for use, the product should be used to treat only seeds of agricultural crops. It is believed that this strain colonizes developing root systems and competes with pathogens that infect the roots. Data on application for processing leaves are not given.

The list below is a list of species B. segeiz that can be used in the present invention.

V. Szegeih

ATCC 55675 (BP01)

ATSS 13824 1С1В 2600 (VasShiz Segeiz Var. Yiogezsepz)

ATCC 14603

ATCC 15816 ATCC 15817 ATCC 13472 ATCC 14737 ATCC 9592 (WasSiz tePepz).

ATCC 27877

ATSS 19637

ATCC 11950

ATCC 23261

ATCC 6464

ATCC 11773 (Egypt Aghgozeris).

ATCC 10876

ATSS 10987

ATCC 4342 (WASShIZ 1ASICIO).

ATCC 21182

ATCC 7004 (Vaschiz alo, asChz).

ATSS 10702

ATCC 12480

ATSS 49063

ATCC 2 (WashSiz AGP)

ATSS 19265 (Vasziz segeiz zizr. And! Ezy)

ATCC 21634

ATCC 12826

ATCC 21768

ATCC 21769

ATCC 21771

ATCC 21772

ATCC 9139

ATCC 21928

ATCC 27522

ATCC 31430

ATCC 43881

ATSS 246 (Waschiz 1as6togbiz).

ATSS 21770 (VasShiz Segeiz).

ATCC 21929 (VasShiz Segeiz).

ATSS 13367

ATCC 31429

ATCC 31293

ATCC 21366 (Vasziz soadi! Apz)

ATSS 25621 (VasShiz tebiza)

ATSS 7039 (Wasziz Teyepz)

ATSS 14893 (VasShiz ziYShz).

ATCC 9818 (WASShIZ 1ASIZ)

ATSS 33018

ATSS 33019

ATCC 55055

ATCC 53522

ATSS 13366

ATCC 7064 (WashShiz $ 1Ateptics)

ATSS 11949

ATSS 10792 (Wasscizsegyz)

ATCC 27348

ATSS 23260 (VasShiz ebbogyZhpos)

ATCC 13061

ATCC 13062 (WasShiz tegálepit)

ATCC 25972

ATCC 14579

ATSS 19646

ATSS 49064

ATCC 11778

ATCC 39152

ATSS 19146 ΡϋΑ З1гат PC1 818 (Rzeibotopaz sp.)

Preferred B. segeiz strain for use in the present invention includes ATCC strain No. 55675. In another embodiment, B. Segeiz strain for use in the present invention has characteristics like ATCC No. 55675. In yet another embodiment, for use in the present invention strain B. segeis has characteristics the same as ATCC No. 55675.

The list below is a list of species of B. shedler that can be used in the present invention.

B, Mezakait

ATCC 33166

ATCC 33167

ATCC 33168

ATCC 33169

ATSS 12872

ATCC 33164

ATCC 11478

ATCC 33165

ATCC 11561

ATSS 11561a

ATCC 115616

ATCC 11561c

ATCC 115616

ATSS 11561e

ATCC 6458

ATCC 6459

ATCC 14946

ATCC 27327

ATSS 33729

ATCC 9885

ATCC 13639

ATCC 13632

ATCC 15374

ATSS 49099

ATCC 49096

ATCC 43725

ATSS 19136

ATCC 25848

ATCC 4531

ATSS 13402

ATCC 15046

ATCC 15047

ATCC 25300

ATSS 19380

ATSS 13368

ATCC 21181

ATCC 35985

ATCC 39383

ATCC 11562

ATSS 15127

ATCC 15128

ATCC 7703

ATCC 14945

ATSS 15177

ATSS 19218

ATCC 25833

ATCC 15450

ATSS 19135

ATSS 19137

ATCC 21916

ATCC 8245

ATCC 15781

ATCC 31294

ATSS 15117

ATSS 15118

ATSS 19160

ATSS 19161

ATSS 89

ATCC 21209

ATCC 35076

ATSS 49098

ATSS 39118

ATCC 7051

ATSS 7052

ATSS 10778

ATCC 8011

ATCC 7056

ATCC 14581

ATCC 13062 ATCC 49097 ATCC 19213 ATCC 71 ATCC 35075 ATCC 21603 ATCC 21738 ATCC 72 ATCC 21737 ATCC 49095 ATCC 15451

Active ingredients

Used in the present invention, the effective active ingredients can be selected from a wide variety of compounds, the action of which is carried out as a result of their participation in the metabolism of the treated plants or as a result of the impact on its stage. As materials, it is desirable to choose active ingredients that do not degrade or, in some way, their activity does not become lower during long-term storage under conditions that are used to store the component of the reinforcing additive containing the bacterium in the form of spores.

The general expression of a plant growth regulating agent includes a number of active ingredients that exert their effects on plants in various ways. In general, plant growth regulators include plant growth retardants, plant growth enhancers, and herbicides.

Suitable plant growth enhancing agents for use in the present invention include plant growth hormones, such as at least one of the 84 identified gibberillins, such as CA ₃ , OA ₄ , OA ₅ , OA _7, and OA ₉ , which are preferred; cytokines (for example, ziatin, kinetin, benzyladenine, dihydrozeatin and isopentyl adenine); auxins (for example, indole acetic acid (1AA), indole butyric acid (1BA) and naphthalene acid (NOA)); sodium ortonitrophenolate sodium; paranitrophenol sodium; 5-nitrogvayakolyat sodium; and polyhydroxycarboxylic acids containing 2, 4, 5 and 6 carbon atoms; etephon; and various nitrogen or phosphorus fertilizers.

Suitable plant growth inhibitors for use in the present invention include both chlormequat chloride, mepiquat chloride, and maleic hydrazide and its esters. Such plant growth regulators affect the metabolism of plants and shift them in the direction of increasing or slowing growth. All such agents can be used in accordance with the application standards and expiration dates indicated on the label of the product by its manufacturer.

Herbicides include triazines (eg, atrazine), urease, glyphosate, sulfosate, glyphosinate, and sethoxydim.

Suitable system agents, whose use is manifested in the improvement of absorption processes, transport, and assimilation processes, include systemic pesticides and systemic fungicides. Systemic agents that are suitable for treating plants in the present invention include 1nabal aba, insecticidal aldicarb, acephate, carbofuran, dimethoate, forate, and terbufos.

Systemic fungicides that will benefit from use as mixtures of the present invention include tridemorph, metalaxyl, iprodione, fosetile-aluminum, thiophanate, benomyl, triadimefon, carboxin, hydroxycarboxine, carbendazim, thiabendazole, ethirimol, bupirimate, and dimethyrimol.

Plants that can be processed using this invention include virtually any plants growing in the soil and influenced by effective agriculturally active ingredients.

Representative plants include crops related to cereals (for example, corn, wheat, legumes), sorghum, desirable and undesirable herbs, weeds, etc.

The application of the invention may be useful for increasing the number of developing fruits in plants that produce fruit bearing sites on which the fruit will develop. Such plants preferably include any of the immature agricultural products and, in particular, cotton, pulses, peanuts, grapes, apple trees, citrus fruits (for example, lemon, lime, orange, grapefruit), berries (for example, strawberries, blackberries, raspberries), tuberous (for example, potatoes, sweet potatoes), breads, cereals (for example, wheat, rice, rye), tomatoes, onions, and also watermelons, cucumbers and cantaloupe.

Mode of application

Compositions of the present invention may take the physical form of a liquid, emulsion, suspension, solid granules, aggregate or mixture of granules (for example, solid particles of active ingredients on inert carrier particles). The use of each physical form for treating plant leaves will, as a rule, be carried out using the technique used for such purposes.

The strains of gram-positive bacteria can be used in the component of improving, enhancing additives in the form of cells, spores, cultures or suspensions. In the form of a liquid or dispersion, the enhancer is placed in a spray tank or other container for storing the liquid and its distribution as a stable aqueous solution in which the equivalent concentration of spores is in the range of approximately 300,000 colony forming units per milliliter (CFU / ml), preferably 1 5 million CFU / ml, preferably from about 1 million to 1.2 million CFU / ml, in order to obtain a composition that is used for treating plant leaves in the range of about 0.1 x 10 ¹⁰ CFU / acre to 100 x 10 ¹⁰ CFU / acre, preferably at rates of approximately 0.1 x 10 ¹⁰ CFU / acre to 10 x 10 ¹⁰ CFU / acre, most preferably at rates of approximately 0.5 x 10 ¹⁰ CFU / acre (0.5 ounces of liquid / acre concentrate) up to 8 x 10 ¹⁰ CFU / acre (2 fluid ounces / acre concentrate). Optionally and preferably in order to simultaneously apply both components, the spray tank will also contain an ingredient of an agriculturally effective active ingredient.

Solid forms of the components before distribution can be mixed in a dry form or formed in the form of aggregates. In order to improve dispersion, particle size reduction, leaf adhesion, etc., one or more conventional adjuvants may be added.

Specific application rates may vary to some extent depending on the method by which the solution should be applied to the surface of the plants. For example, in the case of air spraying, the dilution rates used and the quantities used will be different from those used in the case of spraying from the ground (“out”), hand spraying or spreading granules. For use can be used traditional equipment. If desired, the component of the enhancement agent can be mixed together with other plant treating agents and used simultaneously with them or can be used in a separate processing step. Application for processing leaves is the preferred method to increase the number of fruit bearing sites on fruit bearing plants.

The concentrate can also be used to prepare ready-to-use mixes. So, in prepared form, in a packaged container, the amount of reinforcing, improving additive is approximately from 150,000 to 600,000 CFU / ml, in addition, the corresponding conditions in which the improving additive is located, provide storage of the bacterial component in the form of spores, which will begin to germinate after their application.

Using a pH below 7 (ie, acidic) for many bacteria will ensure the preservation of these bacteria in the form of spores. If necessary, any traditional acidic agents or buffers can be used to provide a suitable acidic pH, which will guarantee storage stability (preferably, they are food type or those that are classified by E.8. Ak 8a &). In such an acidic environment, spores remain stable and exhibit good storage stability. When the ready-to-use mixture is diluted for subsequent use, the pH of the solution will need to be raised to a value above 1, as a result of which the bacteria of the improving additive will revive and vegetative colonies will appear. As a result, the bacteria will begin to multiply on the surface of the treated plants and facilitate the movement, or move agriculturally effective active ingredients.

The bacterium-containing component of the enhancer can be used as a separate treatment or simultaneously with a number of other effective active agricultural ingredients. Suitable effective active ingredients include plant growth enhancers, plant growth retardants, herbicides, systemic insecticides, and systemic fungicides. Preferably, if the composition is a combination of either an agent that slows down plant growth, or a herbicide with an improver containing essentially pure B. c. Strain strain, B. segeic strain, or ATCC 55675 (BP01), applied using a standard of at least 0 , 1 x 10 ¹⁰ CFU / acre.

It is particularly preferable that a gibberillin-free enhancer containing bacteria LacSyc is used to treat the leaves of cotton plants at the same time that the plants are treated with mepiquat chloride. Treatment using a tank containing an aqueous mixture containing mepiquat chloride and an improved additive containing bacteria (preferably ATCC 55675) is a convenient method for the simultaneous use of components. Initially mixed mepiquat chloride and a bacteria-enhancing additive can be easily preserved (unchanged) if the pH of the medium is in the range of 4-6.5, most preferably in the range of 56.5.

It should be noted that in the process of preparing the forms according to the present invention, it is not desirable to use compositions comprising compounds that have opposite effects on plant metabolism. For example, mepiquat chloride is commonly used to process plant leaves to inhibit the effects of growth hormones and retard vegetative growth of a plant. Preferably, these plant growth hormones are not used in form preparation, since the effects of mepiquat chloride and growth hormones are incompatible with plant metabolism, reduce the effect of mepiquat chloride and lead to incompatible results. According to the present invention, however, the combination of mepiquat chloride and ATCC 55675 is compatible, and the plants treated with such a composition produce more fruits (product), have a healthier growth and a higher resistance to diseases. Despite the fact that there is no specific theory regarding the effect of the treatment process and the combined use of the improving additive and mepiquat chloride containing cotton on cotton, it appears that bacteria influence the plant growth mechanism in such a way that seed boxes are retained on fruit bearing sites 1 and 2, as well as the development and preservation of fruit-bearing areas on the shoots, which are usually vegetative, resulting in an increase in the total number of seed pods.

In the present invention, the treatment with mepiquat chloride is carried out using conventional application rates and at the growth stages of cotton plants, which are traditionally processed. Traditionally, mepiquat chloride standards are approximately 60 g / acre (25 g / acre), which corresponds to approximately 1-16 ounces per acre, while application rates range from approximately 2.5 g / acre (1.0 g / hectare ) in the case of using 2 ounces / acre of a 4.2 wt.% solution up to 10 g / acre (4.1 g / hectare) in the case of using 8 ounces / acre of the same 4.2 wt.% solution. If mepiquat chloride is used for a higher or lower purification and / or activity, then specific application rates should be increased or decreased according to a change in the usual activity of mepiquat chloride.

Examples

Example 1

A water mixture of 4.2 wt.% Mepiquat chloride and 560000 CFU / ml of B. segeic (laboratory sample BP01, ATCC 55675) was prepared in a 1: 1 volume ratio. This solution was applied using a standard spraying method in the four treatments of leaves of growing cotton plants in control plots of field A (10 of 50 acres in the fields of Mississippi) and field B (8 of 60 acres in the fields of Tennessee). Field A was treated with a single dose, the rate of which was 4 ounces of fluid per acre, after which, after 5 weeks, the treatment was followed with a rate of 8 ounces of fluid per acre.

Field B was processed in another mode. The rates that were applied in the first three treatments of Field B were 4 oz of fluid per acre. (The second treatment followed 24 days after the first. The third treatment followed 6 days after the second.) The final treatment was performed 11 days after the third treatment, and the value of the norm used in it was 8 ounces of fluid per acre. In all cases, the control fields were treated with the same standards, but using only mepiquat chloride.

After processing in the fields A and B, representative samples were selected by hand. All deviations detected were attributed to the control treatment of mepiquat chloride only.

The initial and final height of treated cotton plants located in field A was compared with the initial and final height of treated cotton plants located in field B. The total height of plants located in the control fields was compared with the total height of plants treated according to the present invention. Values are given in table. one.

Table 1

Field Initial height (dm) Final height (dm) A - control 293 601 A - processed 291 597 B - control 338 580 B - processed 334 636

During the growth period, differences between control and treated plants were easily observed. In those plants that during the first months of fruit development contained only fruit bearing grounds, in those places where the primary fruit shoots disappeared, an unusually high amount of additional fruit bearing shoots were observed, the sizes of which were also unusually increased. Many of them were as large or nearly as large as the primary fruit-bearing shoots and contained fruits up to the stage of the first flowering. Additional fruits were also found in the branches of the fruiting shoots from the main stem.

Since the flowering stage turned out to be prolonged, and before the flowering stage only seed boxes were found in the plants or places where the development of the fruit had not yet begun, among other things, an unexpectedly large number of cases of re-ripening of fruits were observed. Repeated development was observed in the stages of formation of areas, flowering, and the formation of small seed pods, and also in the stage the seed pods are open (rarely). Despite the fact that re-flowering occurred on both control and treated plants, the parameters studied were higher in treated plants.

Approximately 3-4 weeks before the end of the growth period, a study was conducted on the plants, aimed at studying the number of fruit bearing areas, the number of seed pods at nodes 1 and 2, and the type of seed pods. In tab. 2 presents the results of such a study.

table 2

Field Fruiting areas Seed pods % Opened seed pods Opened + green seed boxes Other seed pods A - control 240 115 48 73 270 25 A - processed 292 155 53 173 359 103 In-control 344 207 60 - - 81 B - processed 366 234 64 - - 163

By the end of the control period, 20 plants were investigated in field B. Plants were cut. All plants in the control group, and only 7 plants in the group in which the plants were treated, did not contain seed bolls. However, the young fruits of the treated plants were larger and older than the young fruits of plants from the control group. This indicates the fact that the growth rate of the treated plants was higher and more favorable. Compared to the total number of 238 seed pods in the control group, the total number of 265 seed pods in the treated plants was higher by 11%.

In addition, the treated plants showed an increase in the number of fruits on vegetative shoots in the ratio of 88: 51, an increase of approximately 73%. Treated plants also produced an increased number of fruits in other places, i.e., on fruit bearing shoots in positions 3 and above, in a ratio of 86: 50 (an increase of 72%). The investigated weight (cotton seeds) of the fruits of 20 plants in the treated plants was also more, 1796 g compared to 1456 g (an increase of 23%).

It could be expected that the appearance of additional fruits will lead to the fact that additional parts of the plant and young fruits will deplete the nutrient system of the plants. Bolls will unfold faster, but this will have an adverse effect on the amount of the harvested crop. However, this did not happen. The growth rate of the treated plants was higher and they grew under conditions more healthy than the plants from the control group.

To reduce the deviation, all fibers from the fields under study were collected manually, including not fluff fibers from partially open seed pods. Such unspotted fibers are usually found in seed bolls, which never properly open and are located in the lower part of the plant, or in the newly opened boxes, which are located in the upper part of the plant. In tab. 3 shows the weight of the cotton seed, as well as the number of seed pods per row of cotton plants 10 feet long; data are given for plants that grew in both the treated and control fields.

Table 3

Field Cotton seeds (g) Number of green seed pods A - control 5322 97 A - processed 6287 0 B - control 4532 175 B - processed 5058 42

The test results showed that the use of mepiquat chloride according to the invention simultaneously with the enhancement additive containing ATCC 55675 results in a higher yield of cotton and healthier plants, compared to when only mepiquat chloride is used.

Example 2

In order to determine whether BP01 will affect all those weed plants that are believed not to be destroyed by the use of triazines, the VasSHik segeik BP01 strain (ATCC 55675) was used in combination with the widely used herbicide, atrazine. Atrazine is used to provide long-term seasonal control over cereals, sorghum, and other crops using the estimated norm value corresponding to 2 pounds of active ingredient per acre. It is believed that with such high enough rates used in this example, atrazine has the ability to provide non-selective suppression of weeds.

Three repetitions of each test were carried out on sandy soil on randomly selected model sites measuring 12 feet x 25 feet. By the time of processing, the height of weed plants was 2-6 inches (5-15 cm). Atrazine was applied as an aqueous solution at rates equivalent to either 1 or 2 pounds of active ingredient per acre. BP01 concentrate was added to atrazine in an amount equivalent to either 0.5 or 1 ounce of fluid per acre (0.5 - 1 x 10 ¹⁰ CFU per acre). For comparison, a crop oil concentrate was used (about 85% paraffin oil and about 17% surfactant), since this oil is a traditional additive used with triazine herbicides to increase their strength of action. In tab. 4-8 shows the values of the degree of control over the plants of NopDa Rykleu (Table 4), Bi11Sgakk (Table 5), WegszDaSgakk (Table 6), Iodine Eepie1 (Table 7) and Rpshgok (Table 8).

Table 4. Popda Puigueu

Treatment % Control after processing four of the day 9 days 16 days 23 days Control 0 0 0 0 Atrazine (1 lb.) 3 33 42 45 Atrazine (2 pounds) 17 57 72 83 Atrazine (1 lb.) + agricultural crop oil concentrate (1 gallon) 28 62 68 68 Atrazine (1 lb.) + 0.5 oz. BP01 27 43 60 60 Atrazine (1 lb.) + 1 oz. BP01 22 53 65 67

Table 5. Wi11 (.ggakk

Treatment % Control after processing four of the day 9 days 16 days 23 days Control 0 0 0 0 Atrazine (1 lb.) ten 40 40 55 Atrazine (2 pounds) 25 70 80 90 Atrazine (1 lb.) + agricultural crop oil concentrate (1 gallon) 25 40 65 60 Atrazine (1 lb.) + 0.5 oz. BP01 35 40 55 63 Atrazine (1 lb.) + 1 oz. BP01 thirty 60 80 90

Table 6. Vegtiba Sgas

Treatment % Control after processing four of the day 9 days 16 days 23 days Control 0 0 0 0 Atrazine (1 lb.) 0 ten 15 15 Atrazine (2 pounds) eight 15 22 27 Atrazine (1 lb.) + agricultural crop oil concentrate (1 gallon) 25 27 thirty 43 Atrazine (1 lb.) + 0.5 oz. BP01 7 20 37 68 Atrazine (1 lb.) + 1 oz. BP01 ten 23 53 63

Table 7. Roa Geppe1

Treatment % Control after processing four of the day 9 days 16 days 23 days Control 0 0 0 0 Atrazine (1 lb.) 15 40 55 60 Atrazine (2 pounds) 17 55 70 95 Atrazine (1 lb.) + agricultural crop oil concentrate (1 gallon) 25 75 93 98 Atrazine (1 lb.) + 0.5 oz. BP01 33 88 96 99 Atrazine (1 lb.) + 1 oz. BP01 thirty 90 97 100

Table 8. Rpthoze

Treatment % Control after processing four of the day 9 days 16 days 23 days Control 0 0 0 0 Atrazine (1 lb.) ten 40 50 60 Atrazine (2 pounds) thirty 65 70 80 Atrazine (1 lb.) + agricultural crop oil concentrate (1 gallon) 20 83 88 93 Atrazine (1 lb.) + 0.5 oz. BP01 20 60 72 88 Atrazine (1 lb.) + 1 oz. BP01 20 70 65 80

From tab. 4-8 shows that, as a rule, BP01 enhances the effectiveness of atrazine on day 23 after treatment. As a result of using 1 pound of atrazine together with the bacterium Lacisulis, a higher degree of control was obtained compared to that in the case of using 2 pounds of atrazine in treating the plants of Vegtiba Dhazz, Iodine Repie1 and Rptgose, and in the treatment of the plants of Vi11 Sgasz in both cases the same was obtained degree of control. And only when treating the plants of Nopb Reize 1 lb / acre of atrazine with BP01, a lower degree of control was obtained compared to the treatment where 2 pounds / acre of atrazine was applied.

As a result of the treatments, it was shown that for all weeds, except for Nopb Rizeley and Rpthoze, the use of BP01 also increased the degree of control of atrazine compared with those treatments that used a mixture of atrazine and agricultural herbs oil concentrate. The existence of such an effect implies the fact that the bacillus bacteria do not act in the same way as surfactants, but provide enhanced efficiency either due to their metabolic activity, or due to the characteristics associated with the transfer of agents with which they are used, or both. in a way.

The above examples are intended only to illustrate the invention and do not limit the scope of the appended claims.

Claims (2)

1. A composition having enhanced agricultural activity, comprising (a) an agriculturally effective active ingredient, wherein said active ingredient is selected from the group consisting of a plant growth regulating agent, herbicide, systemic fungicide and systemic insecticide, and (b) an enhancer for enhancing the activity of said active ingredient, wherein said enhancer contains spores, cultures or suspensions of suitable bacteria from the genus Bacillus or soil bacteria at a pH significantly less than 7, to support anija of said amplifier in the form of spores, provided that the composition does not contain any plant growth hormones when said agent regulating plant growth, is an agent retarding the growth of plants. 2. The composition of claim 1, wherein the plant growth regulating agent includes plant growth retarding agents, plant growth enhancing agents, and herbicides. 3. The composition according to claim 2, in which the plant growth enhancing agent includes plant growth hormones such as gibberillins, cytokines, auxins, sodium orthonitrophenolate, sodium paranitrophenolate, sodium 5-nitrogwaycolate, polyhydroxycarboxylic acids containing 2, 4, 5 and 6 carbon atoms, ethephon, nitrogen or phosphorus fertilizers. 4. The composition of claim 2, wherein the plant growth retarding agent comprises chloromequate chloride, meliquat chloride, maleic hydrazide and its esters. 5. The composition of claim 1, wherein the plant growth regulating agent comprises a herbicide, mepiquat chloride, chlorinquat chloride and ethephon. 6. The composition according to p. 1, in which the specified enhancing additive contains a strain of bacteria from the genus VashShiz. 7. The composition according to p. 1, in which the specified enhancing additive contains B. segeiz or V. tedaegst. 8. The composition according to claim 1, in which the enhancing additive contains ATCC 55675. 9. The composition of claim 1 in non-liquid form, comprising a mixture of (a) an agriculturally effective active ingredient selected from the group consisting of a plant growth inhibiting agent, ethephon, plant growth hormone, naphthalene acetic acid, sodium orthonitrophenolate, sodium paranitrophenolate, sodium 5-nitrogwaycolate polyhydroxycarboxylic acids and herbicide; and (b) an enhancing additive that enhances the activity of said ingredient, said enhancer containing spores or cultures of suitable bacteria of the genus Bacillus or soil bacteria, provided that the composition does not contain plant growth hormones when said growth regulatory agent is an agent that inhibits plant growth. 10. The composition according to claim 9, in which the active ingredient is mepiquat chloride, chloromequate chloride or ethephon, glyphosate or sulfosate. 11. A method of increasing the number of fruiting points on fruiting plants by 1) diluting a composition containing (a) an agriculturally effective active ingredient of a plant growth regulating agent and (b) an enhancing additive to enhance the activity of said active ingredient, said enhancing additive containing spores of suitable bacteria from the genus Bacillus or soil bacteria at a pH of less than 7 or significantly lower to maintain the Vaschiz bacteria in the form of spores, and 2) applying the diluted composition to the leaves of said plant containing fruiting points. 12. The method according to claim 11, wherein said fruiting plant is cotton. 13. The method according to claim 11, in which the specified growth regulating agent is an agent that slows the growth of plants or efefon. 14. The method according to claim 11, in which the specified improving additive contains a strain of bacteria from the genus Vasily. 15. The method according to claim 11, in which the aforementioned improving additive contains B. ziyshz, V. teda! Epts or B. segeiz. 16. The method according to claim 11, in which the specified improving additive contains ATCC 55675. 17. A method of treating plants with an agriculturally effective active ingredient having enhanced activity, comprising applying to the plant (a) an agriculturally active active ingredient that inhibits the growth of plants, ethefon or herbicide and (b) reinforcing additives from spores, cultures or suspensions of suitable bacteria from the genus Bacillus or soil bacteria. 18. The method according to 17, in which the specified agriculturally effective active ingredient is a triazine, glyphosate or sulfosate, mepiquat chloride or chloromequate chloride. 19. The method according to 17, in which the specified reinforcing additive contains a strain of bacteria from the genus Vasily. 20. The method according to 17, in which the specified enhancing additive contains B. segeiz or B. tedaepit. 21. The method according to 17, in which the specified reinforcing additive contains ATCC 55675. 22. The composition according to p. 1, including bacteria from the genus VasShiz in the form of their spores, culture or suspension, as well as an agent that slows the growth of plants, and the composition does not contain ATCC 55675. 23. The composition of claim 22, wherein said growth regulatory agent comprises mepiquat chloride. 24. The composition of claim 23, wherein said bacteria of the genus Bacillus are bacteria of Bacillus arteriosus, Bacillus arteriosus, or Bacillus arteriosus. Eurasian Patent Organization, EAPO Russia, GSP-9 101999, Moscow, Center, M. Cherkassky per., 2/6