DK461084A - IMMOBILIZED 5'-PHOSPHODIESTERASE, METHOD OF PREPARING IT AND ITS APPLICATION - Google Patents

IMMOBILIZED 5'-PHOSPHODIESTERASE, METHOD OF PREPARING IT AND ITS APPLICATION Download PDF

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Publication number
DK461084A
DK461084A DK461084A DK461084A DK461084A DK 461084 A DK461084 A DK 461084A DK 461084 A DK461084 A DK 461084A DK 461084 A DK461084 A DK 461084A DK 461084 A DK461084 A DK 461084A
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DK
Denmark
Prior art keywords
phosphodiesterase
immobilized
preparing
application
nucleic acids
Prior art date
Application number
DK461084A
Other languages
Danish (da)
Other versions
DK461084D0 (en
Inventor
Reinhold Keller
Merten Schlingmann
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of DK461084D0 publication Critical patent/DK461084D0/en
Publication of DK461084A publication Critical patent/DK461084A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/091—Phenol resins; Amino resins

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  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

1. A process for enzymatic hydrolysis of nucleic acids, wherein high molecular nucleic acids are treated with 5'-phosphodiesterase immobilized by means of a diisocyanate on a macroporous anion exchange resin based on phenol/formaldehyde carrying essentially secondary amino groups.
DK461084A 1983-09-27 1984-09-26 IMMOBILIZED 5'-PHOSPHODIESTERASE, METHOD OF PREPARING IT AND ITS APPLICATION DK461084A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19833334847 DE3334847A1 (en) 1983-09-27 1983-09-27 IMMOBILIZED 5'-PHOSPHODIESTERASE, METHOD FOR THE PRODUCTION AND THEIR USE

Publications (2)

Publication Number Publication Date
DK461084D0 DK461084D0 (en) 1984-09-26
DK461084A true DK461084A (en) 1985-03-28

Family

ID=6210136

Family Applications (1)

Application Number Title Priority Date Filing Date
DK461084A DK461084A (en) 1983-09-27 1984-09-26 IMMOBILIZED 5'-PHOSPHODIESTERASE, METHOD OF PREPARING IT AND ITS APPLICATION

Country Status (5)

Country Link
EP (1) EP0141224B1 (en)
JP (1) JPS6094087A (en)
AT (1) ATE28897T1 (en)
DE (2) DE3334847A1 (en)
DK (1) DK461084A (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173418A (en) * 1985-05-10 1992-12-22 Benzon Pharma, A/S Production in Escherichia coli of extracellular Serratia spp. hydrolases
IL78703A (en) * 1985-05-10 1993-02-21 Benzon Alfred Method of producing extracellular enzymes, the enzymes produced thereby and compositions containing them; a hybrid plasmid and a dna fragment comprising dna encoding the enzymes; a microorganism harbouring the plasmid; and a method for removing nucleic acids from biological materials
DE4039415A1 (en) 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Recombinant proteins without N-terminal methionine gp. - which are G-CSF derivs. useful to treat cancer and in bone marrow transplants, etc.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56140890A (en) * 1980-04-04 1981-11-04 Sumitomo Chem Co Ltd Immobilized lactase and its preparation
JPS5736986A (en) * 1980-08-13 1982-02-27 Tanabe Seiyaku Co Ltd Immobilized aminoacylase agent and its preparation
SE450834B (en) * 1980-11-19 1987-08-03 Biocompat Inc SET TO BIND A PROTEIN TO A CARRIER CONTAINING HYDROXYL GROUPS

Also Published As

Publication number Publication date
DK461084D0 (en) 1984-09-26
DE3465337D1 (en) 1987-09-17
DE3334847A1 (en) 1985-04-04
EP0141224B1 (en) 1987-08-12
JPS6094087A (en) 1985-05-27
ATE28897T1 (en) 1987-08-15
EP0141224A1 (en) 1985-05-15

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