DK432987A - PROCEDURE FOR IMPROVING EXPRESSION OF EUKARYOT PROTEIN - Google Patents

PROCEDURE FOR IMPROVING EXPRESSION OF EUKARYOT PROTEIN

Info

Publication number
DK432987A
DK432987A DK432987A DK432987A DK432987A DK 432987 A DK432987 A DK 432987A DK 432987 A DK432987 A DK 432987A DK 432987 A DK432987 A DK 432987A DK 432987 A DK432987 A DK 432987A
Authority
DK
Denmark
Prior art keywords
protein
cdna
eukaryot
procedure
improving expression
Prior art date
Application number
DK432987A
Other languages
Danish (da)
Other versions
DK432987D0 (en
Inventor
Guenter Schumacher
Peter Hirth
Peter Buckel
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of DK432987D0 publication Critical patent/DK432987D0/en
Publication of DK432987A publication Critical patent/DK432987A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/03—Automatic limiting or abutting means, e.g. for safety
    • A61B2090/033—Abutting means, stops, e.g. abutting on tissue or skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/06—Measuring instruments not otherwise provided for
    • A61B2090/062—Measuring instruments not otherwise provided for penetration depth

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Networks Using Active Elements (AREA)
  • Saccharide Compounds (AREA)

Abstract

The expression of a eucaryotic protein is improved by introducing the cDNA coding sequence of a eucaryotic gene at least one intron naturally occurring in the corresponding gene. This cDNA is then cloned in procaryotes, the cloned cDNA is recovered and if necessary introduced into a eucaryotic cell which translates the cDNA into the desired protein.
DK432987A 1985-12-19 1987-08-19 PROCEDURE FOR IMPROVING EXPRESSION OF EUKARYOT PROTEIN DK432987A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19853545126 DE3545126A1 (en) 1985-12-19 1985-12-19 METHOD FOR IMPROVING THE EXPRESSION OF EUKARYON PROTEIN
PCT/EP1986/000763 WO1987003904A1 (en) 1985-12-19 1986-12-19 Process for improving the expression of a eucaryotic protein

Publications (2)

Publication Number Publication Date
DK432987D0 DK432987D0 (en) 1987-08-19
DK432987A true DK432987A (en) 1987-08-19

Family

ID=6288962

Family Applications (1)

Application Number Title Priority Date Filing Date
DK432987A DK432987A (en) 1985-12-19 1987-08-19 PROCEDURE FOR IMPROVING EXPRESSION OF EUKARYOT PROTEIN

Country Status (14)

Country Link
EP (2) EP0227064A1 (en)
JP (1) JPS62502942A (en)
KR (1) KR880700855A (en)
AT (1) ATE67242T1 (en)
AU (1) AU600253B2 (en)
DE (2) DE3545126A1 (en)
DK (1) DK432987A (en)
ES (1) ES2026206T3 (en)
FI (1) FI873572A0 (en)
GR (1) GR3003301T3 (en)
IL (1) IL80960A0 (en)
PT (1) PT83968B (en)
WO (1) WO1987003904A1 (en)
ZA (1) ZA869487B (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005466A2 (en) * 1987-01-15 1988-07-28 Codon Tandem gene eukaryotic expression vectors
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
DE3730246A1 (en) * 1987-09-09 1989-06-01 Boehringer Mannheim Gmbh EXPRESSION VECTOR AND METHOD FOR EXPRESSING HETEROLOGICAL PROTEINS IN SUCTION CELLS
CA2018676C (en) * 1989-06-20 2003-05-06 Christie A. Kelton Novel heteropolymeric protein production methods
FR2650838A1 (en) * 1989-08-09 1991-02-15 Transgene Sa FACTOR IX EXPRESSION VECTORS IN A SUPERIOR EUKARYOTE CELL, PROCESS FOR THE PREPARATION OF FACTOR IX BY TRANSGENIC ANIMALS, AND FACTOR IX OBTAINED
EP0451700A1 (en) * 1990-04-10 1991-10-16 Miles Inc. Recombinant APP minigenes for expression in transgenic mice as models for Alzheimers's disease
AU1180692A (en) * 1991-01-29 1992-08-27 Teijin Limited Human protein c expression vector
US6627615B1 (en) 1991-12-17 2003-09-30 The Regents Of The University Of California Methods and compositions for in vivo gene therapy
US5756353A (en) * 1991-12-17 1998-05-26 The Regents Of The University Of California Expression of cloned genes in the lung by aerosol-and liposome-based delivery
US5858784A (en) * 1991-12-17 1999-01-12 The Regents Of The University Of California Expression of cloned genes in the lung by aerosol- and liposome-based delivery
US6806084B1 (en) 1992-06-04 2004-10-19 The Regents Of The University Of California Methods for compositions for in vivo gene delivery
IL105914A0 (en) * 1992-06-04 1993-10-20 Univ California Methods and compositions for in vivo gene therapy
US6027888A (en) * 1996-04-05 2000-02-22 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells
EP0816499A3 (en) * 1996-06-27 1999-10-27 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Genomic DNA encoding a polypeptide capable of inducing the production of interferon-gamma
JP2002517180A (en) * 1997-12-05 2002-06-18 ジ・イミユーン・リスポンス・コーポレーシヨン Novel vectors and genes exhibiting increased expression
SE9901339D0 (en) * 1999-04-15 1999-04-15 Astra Ab New expression systems
GB201106380D0 (en) 2011-04-15 2011-06-01 Nokia Corp An apparatus, method, computer program and user interface

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2534273B1 (en) * 1982-10-07 1985-09-13 Pasteur Institut CELL LINES ORIGINATING IN SUPERIOR MAMMALS, PRODUCERS OF GROWTH HORMONE, PREFERABLY HUMAN, AND VECTORS FOR THEIR PRODUCTION
FR2535243B1 (en) * 1982-11-03 1986-04-04 Fives Cail Babcock PROCESS FOR MANUFACTURING A CYLINDER, PARTICULARLY FOR A ROD MILL, AND CYLINDER OBTAINED BY THIS PROCESS
IL71691A (en) * 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
WO1986005514A1 (en) * 1985-03-22 1986-09-25 Chiron Corporation EXPRESSION OF tPA IN MAMMALIAN CELLS
FR2603899B1 (en) * 1986-09-12 1990-07-13 Genentech Inc IMPROVED PROCESS FOR THE EXPRESSION OF RECOMBINANTS

Also Published As

Publication number Publication date
ATE67242T1 (en) 1991-09-15
FI873572A (en) 1987-08-18
PT83968A (en) 1987-01-01
ZA869487B (en) 1987-09-30
IL80960A0 (en) 1987-03-31
DK432987D0 (en) 1987-08-19
JPS62502942A (en) 1987-11-26
FI873572A0 (en) 1987-08-18
EP0227064A1 (en) 1987-07-01
ES2026206T3 (en) 1992-04-16
DE3545126A1 (en) 1987-06-25
EP0250513B1 (en) 1991-09-11
AU600253B2 (en) 1990-08-09
GR3003301T3 (en) 1993-02-17
KR880700855A (en) 1988-04-12
DE3681446D1 (en) 1991-10-17
AU6833387A (en) 1987-07-15
WO1987003904A1 (en) 1987-07-02
PT83968B (en) 1989-01-17
EP0250513A1 (en) 1988-01-07

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