DK350183A - ALKALIC PROTEASE AND PROCEDURE FOR PREPARING THEREOF - Google Patents
ALKALIC PROTEASE AND PROCEDURE FOR PREPARING THEREOF Download PDFInfo
- Publication number
- DK350183A DK350183A DK350183A DK350183A DK350183A DK 350183 A DK350183 A DK 350183A DK 350183 A DK350183 A DK 350183A DK 350183 A DK350183 A DK 350183A DK 350183 A DK350183 A DK 350183A
- Authority
- DK
- Denmark
- Prior art keywords
- degree
- protease
- minutes
- heating
- activity
- Prior art date
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- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
Abstract
An alkaline protease, API-21, has the following physicochemical properties: 1) activity: hydrolyses caseine under basic conditions, has an optimum pH range 10-11 and an optimum temperature range for activity at 45-50 degree C, 2) stability: while no decline in enzyme activity is observed when heating at 40 degree C without substrate for 10 minutes at a pH of 10, a decline in enzyme activity of 90% or more is observed when heating at 50 degree C for 10 minutes at a pH of 10. Inactivation occurs when heating at 50 degree C for 10 minutes at a pH of 8, and complete inactivation is brought about by heating at 60 degree C for 10 minutes at a pH of 8. The protease is stable under basic conditions, in particular in a pH range of 7-11.5, and is stable towards freezing and freeze-drying; and 3) enzyme molecule: the protease is a protein having a molecular weight of approx. 22,000, estimated by gel filtration, an isoelectric point of 7.4, estimated by an electrofocusing method, an absorption peak at 275-282 nm in a UV absorption spectrum, IR absorption characteristics as shown in Fig. 5 and a serine residue in the active site for this residue. This outline protease is prepared by culturing the species NKS-21 belonging to the genus Bacillus. <IMAGE>
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK350183A DK158523C (en) | 1983-07-29 | 1983-07-29 | Alkaline protease and process for its preparation |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK350183A DK158523C (en) | 1983-07-29 | 1983-07-29 | Alkaline protease and process for its preparation |
| DK350183 | 1983-07-29 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| DK350183D0 DK350183D0 (en) | 1983-07-29 |
| DK350183A true DK350183A (en) | 1985-01-30 |
| DK158523B DK158523B (en) | 1990-05-28 |
| DK158523C DK158523C (en) | 1995-12-04 |
Family
ID=8123611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK350183A DK158523C (en) | 1983-07-29 | 1983-07-29 | Alkaline protease and process for its preparation |
Country Status (1)
| Country | Link |
|---|---|
| DK (1) | DK158523C (en) |
-
1983
- 1983-07-29 DK DK350183A patent/DK158523C/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DK158523B (en) | 1990-05-28 |
| DK350183D0 (en) | 1983-07-29 |
| DK158523C (en) | 1995-12-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUP | Patent expired |