DK2870466T3 - Elektrode og anvendelse deraf - Google Patents

Elektrode og anvendelse deraf Download PDF

Info

Publication number
DK2870466T3
DK2870466T3 DK13733454.6T DK13733454T DK2870466T3 DK 2870466 T3 DK2870466 T3 DK 2870466T3 DK 13733454 T DK13733454 T DK 13733454T DK 2870466 T3 DK2870466 T3 DK 2870466T3
Authority
DK
Denmark
Prior art keywords
insulin
electrode
target species
monomers
photopolymerizable
Prior art date
Application number
DK13733454.6T
Other languages
English (en)
Inventor
Jason Davis
Original Assignee
Univ Oxford Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Oxford Innovation Ltd filed Critical Univ Oxford Innovation Ltd
Application granted granted Critical
Publication of DK2870466T3 publication Critical patent/DK2870466T3/da

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/026—Dielectric impedance spectroscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/14532—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring glucose, e.g. by tissue impedance measurement
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/14546—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue for measuring analytes not otherwise provided for, e.g. ions, cytochromes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/1468—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using chemical or electrochemical methods, e.g. by polarographic means
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/1486—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue using enzyme electrodes, e.g. with immobilised oxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/62—Insulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
    • G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
    • G01N27/07—Construction of measuring vessels; Electrodes therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Peptides Or Proteins (AREA)

Claims (12)

1. Fremgangsmåde til fremstilling af en elektrode til anvendelse i en elektrokemisk impedansspektroskopiteknik, hvilken fremgangsmåde omfatter: (a) fastgørelse af fotopolymeriserbare betainmonomerer til den plane overflade af et substrat, hvorved der opnås en ændret overflade af fotopolymeriserbare betainmonomerer placeret derpå; derefter (b) etablering af kontakt mellem den ændrede overflade og yderligere fotopolymeriserbare betainmonomerer, og eventuelt tværbindende monomerer, og fotokemisk polymerising af monomererne, hvorved der genereres en elektrode omfattende polymerer placeret på den plane overflade; hvor fastgørelsen af laget af fotopolymeriserbare betainmonomerer i trin (a) i alt væsentligt er afsluttet inden etablering af kontakt mellem den modificerede overflade og yderligere fotopolymeriserbare betainmonomerer, og eventuelt tværbindende monomerer, og den fotokemiske polymerisering udføres i trin (b).
2. Fremgangsmåde ifølge krav 1, hvor de fotopolymeriserbare betainmonomerer hver omfatter en fotopolymeriserbar carbon-carbon-dobbcltbinding.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor de fotopolymeriserbare betainmonomerer hver omfatter en kvatemær ammoniumkation og en carboxy latgrupp e.
4. Fremgangsmåde ifølge krav 3, hvor de fotopolymeriserbare betainmonomerer har formlen (II)
(H) hvor: Ri og R3 er ens eller forskellige og hver er en Ci- til Cs-alkylengruppe; R2 og R2’ er ens eller forskellige og hver er en Ci- til C5-alkylgruppe; R4 er et hydrogenatom eller et Q- til CValkylgruppc; og X er O eller NH.
5. Fremgangsmåde ifølge krav 4, hvor de fotopolymeriserbare betainmonomerer er udvalgt fra carboxybetainmethacrylat (CBMA), carboxybetainacrylamin (CBAA) og carboxybetainethylacrylat (CBEA).
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 5, der endvidere omfatter (c) fastgørelse af sondemolekyler, der er i stand til specifikt at binde til en målart, til polymererne.
7. Fremgangsmåde ifølge krav 6, hvor målarten er insulin.
8. Elektrode, der kan opnås ved hjælp af fremgangsmåden som defineret i et hvilket som helst af kravene 1 til 7.
9. Fremgangsmåde til detektering af en målart i en elektrokemisk impedansspektroskopiteknik, hvor fremgangsmåden omfatter: (a) etablering af kontakt mellem en elektrode som defineret i krav 8 og et bærermedium, der omfatter målarten; og (b) detektering af et elektrisk signal.
10. Fremgangsmåde ifølge krav 9, der er en fremgangsmåde til detektering af insulin i en non-Faraday elektrokemisk impedansspektroskopiteknik, hvor fremgangsmåden omfatter: (a) etablering af kontakt mellem en elektrode, der kan opnås ved hjælp af en fremgangsmåde som defineret i krav 7, og et bærermedium, der omfatter insulin; (b) detektering af et elektrisk signal; og (c) beregning af koncentrationen af insulin ud fra det elektriske signal, hvor koncentrationen af insulin beregnes ud fra en faseændring i ct elektrisk signal, der fremkommer efter binding af insulinet til sondemolekyleme.
11. Elektrokemisk impedansspektrometer, der omfatter en elektrode som defineret i krav 8.
12. Anvendelse af en elektrode som defineret i krav 8 til detektering af en målart ved hjælp af en elektrokemisk impedansspektroskopiteknik.
DK13733454.6T 2012-07-03 2013-07-02 Elektrode og anvendelse deraf DK2870466T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1211775.0A GB201211775D0 (en) 2012-07-03 2012-07-03 An electrode and use thereof
PCT/GB2013/051749 WO2014006394A2 (en) 2012-07-03 2013-07-02 An electrode and use thereof

Publications (1)

Publication Number Publication Date
DK2870466T3 true DK2870466T3 (da) 2017-08-14

Family

ID=46721798

Family Applications (2)

Application Number Title Priority Date Filing Date
DK13733454.6T DK2870466T3 (da) 2012-07-03 2013-07-02 Elektrode og anvendelse deraf
DK17166598.7T DK3211410T3 (da) 2012-07-03 2013-07-02 Elektrode og anvendelse deraf

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK17166598.7T DK3211410T3 (da) 2012-07-03 2013-07-02 Elektrode og anvendelse deraf

Country Status (6)

Country Link
US (1) US10352891B2 (da)
EP (2) EP3211410B1 (da)
DK (2) DK2870466T3 (da)
ES (2) ES2769288T3 (da)
GB (1) GB201211775D0 (da)
WO (1) WO2014006394A2 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018107143A1 (en) * 2016-12-09 2018-06-14 University Of Pittsburgh-Of The Commonwealth System Of Higher Education Multi-array impedimetric biosensors for the detection of concussion and traumatic brain injuries
WO2018169734A1 (en) * 2017-03-15 2018-09-20 California Institute Of Technology Smart bandage
US11185263B2 (en) * 2017-11-21 2021-11-30 Arizona Board Of Regents On Behalf Of Arizona State University Biosensor array for the detection of analytes
WO2020070486A1 (en) 2018-10-02 2020-04-09 Osler Diagnostics Limited Methods for immunoassays using electrochemical measurement
JP7479627B2 (ja) * 2020-05-29 2024-05-09 国立大学法人広島大学 インスリンの検出方法および検出キット

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6846654B1 (en) 1983-11-29 2005-01-25 Igen International, Inc. Catalytic antibodies as chemical sensors
US6824669B1 (en) * 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
US7435384B2 (en) * 2001-01-08 2008-10-14 Leonard Fish Diagnostic instrument with movable electrode mounting member and methods for detecting analytes
US6861224B2 (en) 2001-11-02 2005-03-01 Fujitsu Limited Protein detecting device
US7943370B2 (en) * 2007-08-23 2011-05-17 Canon Kabushiki Kaisha Structure, target substance detection element and target substance detection kit
CN102337778B (zh) 2010-07-17 2013-09-11 吕锋滨 用连接构件拼接的硬质拼接板块
CN101957375B (zh) 2010-10-15 2013-03-20 扬州大学 呋喃它酮残留物无标记阻抗型免疫传感器制备方法和应用
WO2013013220A2 (en) * 2011-07-20 2013-01-24 University Of Washington Through Its Center For Commercialization Photonic blood typing

Also Published As

Publication number Publication date
GB201211775D0 (en) 2012-08-15
DK3211410T3 (da) 2020-01-20
US20150177180A1 (en) 2015-06-25
US10352891B2 (en) 2019-07-16
WO2014006394A3 (en) 2014-02-27
ES2635638T3 (es) 2017-10-04
WO2014006394A2 (en) 2014-01-09
EP3211410B1 (en) 2019-11-20
EP2870466B1 (en) 2017-04-26
ES2769288T3 (es) 2020-06-25
EP2870466A2 (en) 2015-05-13
EP3211410A1 (en) 2017-08-30

Similar Documents

Publication Publication Date Title
Moreira et al. Smart plastic antibody material (SPAM) tailored on disposable screen printed electrodes for protein recognition: Application to myoglobin detection
Piccoli et al. Redox capacitive assaying of C-reactive protein at a peptide supported aptamer interface
US10830727B2 (en) Electrode and use thereof
EP3250911B1 (en) Quantum capacitance sensing
Wang et al. Molecularly imprinted polymer (MIP) based electrochemical sensors and their recent advances in health applications
DK2870466T3 (da) Elektrode og anvendelse deraf
Aswini et al. Molecularly imprinted poly (4-amino-5-hydroxy-2, 7-naphthalenedisulfonic acid) modified glassy carbon electrode as an electrochemical theophylline sensor
Moreira et al. Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-screen printed electrodes
Tadi et al. Electrochemical detection of epinephrine using a biomimic made up of hemin modified molecularly imprinted microspheres
Dervisevic et al. Boronic Acid vs. Folic Acid: A Comparison of the bio-recognition performances by Impedimetric Cytosensors based on Ferrocene cored dendrimer
US10564154B2 (en) Capacitance spectroscopic method and electrode
EP2844988B1 (en) Electrochemical detection method
Sopousek et al. Blocking the nanopores in a layer of nonconductive nanoparticles: dominant effects therein and challenges for electrochemical impedimetric biosensing
US20150219579A1 (en) Method for detecting autoantibodies
Shervedani et al. Electrochemical determination of calf thymus DNA on Zr (IV) immobilized on gold–mercaptopropionic-acid self-assembled monolayer
Ensafi et al. Molecularly imprinted biosensors for sensitive detection of biomarkers
Xu Optimised label-free biomarker assays with electrochemical impedance spectroscopy