DK2794664T3 - Hybridpolypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem - Google Patents

Hybridpolypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem Download PDF

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DK2794664T3
DK2794664T3 DK12859897.6T DK12859897T DK2794664T3 DK 2794664 T3 DK2794664 T3 DK 2794664T3 DK 12859897 T DK12859897 T DK 12859897T DK 2794664 T3 DK2794664 T3 DK 2794664T3
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gly
thr
ala
polypeptide
ser
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DK12859897.6T
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Tarana Shaghasi
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Novozymes Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Claims (20)

1. Isoleret hybridpolypeptid med cellobiohydrolaseaktivitet, hvilket hybridpolypeptid omfatter: (a) et første polypeptidfragment, som omfatter et katalytisk i cellobiohydrolasedomæne, i den N-terminale ende af hybridpolypeptidet valgt fra gruppen bestående af (i) et polypeptidfragment, der udviser mindst 96% sekvensidentitet med aminosyrerne 19 til 455 ifølge SEQ ID NO: 2; (ii) et polypeptidfragment, som kodes for af et polynukleotid, der udviser mindst 96% sekvensidentitet med nukleotiderne 55 til 1485 ifølge SEQ ID NO: 1 eller cDNA-sekvensen deraf; og (iii) et polypeptidfragment, som omfatter eller består af aminosyrerne 19 til 455 ifølge SEQ ID NO: 2 eller et fragment deraf med cellobiohydrolaseaktivitet; og (b) et andet polypeptidfragment, som omfatter et carbohydratbindingsdomæne i den C-terminale ende af det første polypeptidfragment valgt fra gruppen bestående af (i) et polypeptidfragment, der udviser mindst 96% sekvensidentitet med aminosyrerne 485 til 529 ifølge SEQ ID NO: 4; (ii) et polypeptidfragment, som kodes for i af et polynukleotid, der udviser mindst 96% sekvensidentitet med nukleotiderne 1453 til 1587 ifølge SEQ ID NO: 3; og (iii) et polypeptidfragment, som omfatter eller består af aminosyrerne 485 til 529 ifølge SEQ ID NO: 4 eller et fragment deraf med carbohydratbindingsaktivitet.
2. Hybridpolypeptid ifølge krav 1, hvor det første polypeptidfragment omfatter eller består af aminosyrerne 19 til 455 ifølge SEQ ID NO: 2 eller et fragment deraf med cellobiohydrolaseaktivitet.
3. Hybridpolypeptid ifølge krav 1, hvor det andet polypeptidfragment omfatter eller består af aminosyrerne 485 til 529 ifølge SEQ ID NO: 4 eller et fragment deraf med carbohydratbindingsaktivitet.
4. Hybridpolypeptid ifølge krav 1, som omfatter eller består af aminosyrerne 19 til 455 ifølge SEQ ID NO: 2 som det første polypeptidfragment i den N-terminale ende af hybridpolypeptidet og aminosyrerne 485 til 529 ifølge SEQ ID NO: 4 som det andet polypeptidfragment i den C-terminale ende af det første polypeptidfragment.
5. Hybridpolypeptid ifølge krav 1, som omfatter eller består af det modne polypeptid ifølge SEQ ID NO: 6 eller et fragment deraf med cellobiohydrolaseaktivitet og carbohydratbindingsaktivitet.
6. Hybridpolypeptid ifølge et hvilket som helst af kravene 1-5, som endvidere omfatter et signalpeptid i den N-terminale ende af det første polypeptidfragment.
7. Hybridpolypeptid ifølge krav 6, hvor signalpeptidet er signalpeptidet ifølge SEQ ID NO: 2.
8. Hybridpolypeptid ifølge krav 7, hvor signalpeptidet er aminosyrerne 1 til 21 ifølge SEQ ID NO: 2.
9. Isoleret polynukleotid, som koder for hybridpolypeptidet ifølge et hvilket som helst af kravene 1-8.
10. Værtscelle, som omfatter polynukleotidet ifølge krav 9.
11. Fremgangsmåde til fremstilling af et hybridpolypeptid med cellobiohydrolaseaktivitet, hvilken fremgangsmåde omfatter: (a) dyrkning af værtscellen ifølge krav 10 under betingelser, som er egnede til ekspression af hybridpolypeptidet; og eventuelt (b) indvinding af hybridpolypeptidet.
12. Transgen plante, plantedel eller plantecelle, som er transformeret med polynukleotidet ifølge krav 9, der koder for et hybridpolypeptid.
13. Fremgangsmåde til fremstilling af hybridpolypeptidet ifølge et hvilket som helst af kravene 1-8, hvilken fremgangsmåde omfatter: (a) dyrkning af en transgen plante eller en plantecelle, som omfatter et polynukleotid, hvilket polynukleotid koder for hybridpolypeptidet, under betingelser, der er befordrende for fremstilling af hybridpolypeptidet; og eventuelt (b) indvinding af hybridpolypeptidet.
14. Fremgangsmåde til nedbrydning eller omdannelse af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: behandling af det celluloseholdige materiale med en enzymsammensætning i nærværelse af hybridpolypeptidet ifølge et hvilket som helst af kravene 1-8.
15. Fremgangsmåde ifølge krav 14, som endvidere omfatter indvinding af det nedbrudte celluloseholdige materiale.
16. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: (a) forsukring af et celluloseholdigt materiale med en enzymsammensætning i nærværelse af hybridpolypeptidet ifølge et hvilket som helst af kravene 1-8; (b) fermentering af det forsukrede celluloseholdige materiale med en eller flere fermenterende mikroorganismer for at fremstille fermenteringsproduktet; og (c) indvinding af fermenteringsproduktet fra fermenteringen.
17. Fremgangsmåde til fermentering af et celluloseholdigt materiale, hvilken fremgangsmåde omfatter: fermentering af det celluloseholdige materiale med en eller flere fermenterende mikroorganismer, hvor det celluloseholdige materiale er forsukret med en enzymsammensætning i nærværelse af hybridpolypeptidet ifølge et hvilket som helst af kravene 1-8.
18. Fremgangsmåde ifølge krav 17, hvor fermenteringen af det celluloseholdige materiale frembringer et fermenteringsprodukt.
19. Fremgangsmåde ifølge krav 18, som endvidere omfatter indvinding af i fermenteringsproduktet fra fermenteringen.
20. Totalmedieformulering eller celledyrkningssammensætning, som omfatter hybridpolypeptidet ifølge et hvilket som helst af kravene 1-8.
DK12859897.6T 2011-12-22 2012-12-21 Hybridpolypeptider med cellobiohydrolaseaktivitet og polynukleotider, som koder for dem DK2794664T3 (da)

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CN2011084419 2011-12-22
PCT/CN2012/087212 WO2013091577A1 (en) 2011-12-22 2012-12-21 Hybrid polypeptides having cellobiohydrolase activity and polynucleotides encoding same

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US (2) US9481897B2 (da)
EP (1) EP2794664B1 (da)
CN (1) CN104039834B (da)
BR (1) BR112014015242B1 (da)
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WO (1) WO2013091577A1 (da)

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Publication number Priority date Publication date Assignee Title
US10221406B2 (en) 2013-03-08 2019-03-05 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
EP3594335B1 (en) 2014-09-05 2024-05-01 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
WO2016040265A1 (en) 2014-09-08 2016-03-17 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
EP3739045A3 (en) 2015-02-24 2021-03-10 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
WO2017050242A1 (en) * 2015-09-22 2017-03-30 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
BR112018015184B1 (pt) 2016-02-19 2022-09-06 Intercontinental Great Brands Llc Processos para criar múltiplas correntes de valor a partir de fontes de biomassa

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EP1752533A1 (en) 2005-08-12 2007-02-14 Institut National de la Recherche Agronomique Fusion proteins between plant cell-wall degrading enzymes, and their uses
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
EP2361311B1 (en) * 2008-11-21 2017-01-18 Lallemand Hungary Liquidity Management LLC Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose
ES2549328T3 (es) * 2010-02-11 2015-10-27 Süd-Chemie Ip Gmbh & Co. Kg Enzimas celulasas optimizadas

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US20170022489A1 (en) 2017-01-26
CN104039834A (zh) 2014-09-10
US9481897B2 (en) 2016-11-01
EP2794664B1 (en) 2017-05-10
EP2794664A4 (en) 2015-08-19
US20140377827A1 (en) 2014-12-25
US9969994B2 (en) 2018-05-15
BR112014015242A2 (pt) 2020-06-30
WO2013091577A1 (en) 2013-06-27
EP2794664A1 (en) 2014-10-29
CN104039834B (zh) 2018-09-14

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