DK2730660T3 - Værktøjer og fremgangsmåde til detektering og kvantificering af genetisk forskellige HIV-1-, SIVcpz- og SIVgor-vira - Google Patents

Værktøjer og fremgangsmåde til detektering og kvantificering af genetisk forskellige HIV-1-, SIVcpz- og SIVgor-vira Download PDF

Info

Publication number
DK2730660T3
DK2730660T3 DK12192087.0T DK12192087T DK2730660T3 DK 2730660 T3 DK2730660 T3 DK 2730660T3 DK 12192087 T DK12192087 T DK 12192087T DK 2730660 T3 DK2730660 T3 DK 2730660T3
Authority
DK
Denmark
Prior art keywords
hiv
samples
sivgor
assay
probe
Prior art date
Application number
DK12192087.0T
Other languages
English (en)
Inventor
Martine Peeters
Lucie Etienne
Original Assignee
Inst Rech Pour Le Développement Ird
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Rech Pour Le Développement Ird filed Critical Inst Rech Pour Le Développement Ird
Application granted granted Critical
Publication of DK2730660T3 publication Critical patent/DK2730660T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Claims (6)

1. Oligonukleotid af sekvens SEQ ID N° 1: 5'-CTAGAGATCCCTCAGA - 3', eller den komplementære sekvens deraf af sekvens SEQ ID N° 2: 5'- T CT GAG G GAT CTCTAG - 3', der er egnet som sonde til detektering og kvantificering af alle HIV-1 cirkulerende former og deres precursorer SIVcpz/SIVgor.
2. Sonde ifølge krav 1, bestående af en Taqman-sonde med en fluorescerende markør ved den 5'-position og en quencher ved den 3'-position.
3. Sæt af primere/sonde, hvor primerne består af en fremadprimer af sekvens SEQ ID N°3: 5'- SSCTCAATAAAGCTTGCC-3' , hvor S repræsenterer G eller C og en bagudprimer af sekvens SEQ ID N°4: 5'-AAAATCTCTAGCAGTGGCGCC-3' eller den komplementære sekvens deraf af sekvens SEQ ID N°5:
5 - GGCGCCACTGCTAGAGATTTT - 3', og sonden af sættet er i henhold til krav 1 eller 2.
4. Fremgangsmåde til detektering og kvantificering af HIV-1 cirkulerende former og deres precursorer SIVcpz/SIVgor i en testprøve, hvilken fremgangsmåde omfatter trinnene - dannelse af en reaktionsblanding omfattende et sæt af primere/sonde ifølge krav 3 og en testprøve indeholdende en målsekvens af HIV-1 eller precursorer deraf SIVcpz/SIVgor, - placering af reaktionsblandingen under amplificeringsbetingelser til dannelse af et amplificeringsprodukt og detektering af produkt-/sondehybriden som en indikation af tilstedeværelsen af viraene i prøven - dannelse af en hybrid mellem amplificeringsproduktet og en sonde - detektering af hybriden som en indikation af tilstedeværelsen af HIV-l/SIVcpz/SIVgor i testprøven.
5. Kits til detektering og kvantificering af HIV-1 cirkulerende former og deres precursorer SIVcpz/SIVgor i en testprøve, omfattende et sæt af prime-re/sonde ifølge krav 3 i egnede beholdere og forsynet med yderligere reagenser såsom amplificeringsreagenser.
DK12192087.0T 2012-11-09 2012-11-09 Værktøjer og fremgangsmåde til detektering og kvantificering af genetisk forskellige HIV-1-, SIVcpz- og SIVgor-vira DK2730660T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20120192087 EP2730660B1 (en) 2012-11-09 2012-11-09 Tools and method for the detection and quantification of genetically diverse HIV-1, SIVcpz and SIVgor viruses

Publications (1)

Publication Number Publication Date
DK2730660T3 true DK2730660T3 (da) 2015-08-24

Family

ID=47146263

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12192087.0T DK2730660T3 (da) 2012-11-09 2012-11-09 Værktøjer og fremgangsmåde til detektering og kvantificering af genetisk forskellige HIV-1-, SIVcpz- og SIVgor-vira

Country Status (8)

Country Link
US (1) US9765406B2 (da)
EP (1) EP2730660B1 (da)
AP (1) AP2015008467A0 (da)
DK (1) DK2730660T3 (da)
ES (1) ES2545823T3 (da)
IN (1) IN2015DN03944A (da)
WO (1) WO2014072457A1 (da)
ZA (1) ZA201503376B (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110335638B (zh) * 2019-05-22 2021-11-23 北京安智因生物技术有限公司 一种他汀类用药基因检测报告自动化生成方法及系统
CN111304370B (zh) * 2020-04-22 2023-05-26 广东省实验动物监测所 一种快速检测猴免疫缺陷病毒siv引物、探针及其试剂盒和使用方法
CN112795699A (zh) * 2021-02-09 2021-05-14 南方医科大学 一种rt-raa荧光法检测hiv-1病毒不同亚型通用引物、探针及检测方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69029740T2 (de) * 1989-11-30 1997-07-10 Pharmacia Biotech Ab Neues verfahren zum nachweis einer spezifischen nukleinsäuresequenz in einer zellprobe
US6623920B1 (en) * 1999-07-09 2003-09-23 Gen-Probe Incorporated Detection of HIV-1 by nucleic acid amplification

Also Published As

Publication number Publication date
ZA201503376B (en) 2016-07-27
US20150275316A1 (en) 2015-10-01
US9765406B2 (en) 2017-09-19
EP2730660A1 (en) 2014-05-14
IN2015DN03944A (da) 2015-10-02
AP2015008467A0 (en) 2015-05-31
WO2014072457A1 (en) 2014-05-15
ES2545823T3 (es) 2015-09-16
EP2730660B1 (en) 2015-05-20

Similar Documents

Publication Publication Date Title
Curtis et al. A multiplexed RT-LAMP assay for detection of group M HIV-1 in plasma or whole blood
Tang et al. A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A–H, group O and group N samples
Liszewski et al. Detecting HIV-1 integration by repetitive-sampling Alu-gag PCR
Curtis et al. Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP)
Vandamme et al. Quantification of HIV-1 RNA in plasma: comparable results with the NASBA HIV-1 RNA QT and the AMPLICOR HIV monitor test
Leutenegger et al. Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan® fluorogenic real-time detection system
Damond et al. Plasma RNA viral load in human immunodeficiency virus type 2 subtype A and subtype B infections
Luft et al. HIV-1 viral diversity and its implications for viral load testing: review of current platforms
de Baar et al. Single rapid real-time monitored isothermal RNA amplification assay for quantification of human immunodeficiency virus type 1 isolates from groups M, N, and O
Coertse et al. Improved PCR methods for detection of African rabies and rabies-related lyssaviruses
Redd et al. Identification of HIV superinfection in seroconcordant couples in Rakai, Uganda, by use of next-generation deep sequencing
Demontis et al. HTLV‐1 viral RNA is detected rarely in plasma of HTLV‐1 infected subjects
de Baar et al. One-tube real-time isothermal amplification assay to identify and distinguish human immunodeficiency virus type 1 subtypes A, B, and C and circulating recombinant forms AE and AG
Ocwieja et al. A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes
Simen et al. An international multicenter study on HIV-1 drug resistance testing by 454 ultra-deep pyrosequencing
Anantharajah et al. How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?
Rouet et al. Comparison of the generic HIV viral load® assay with the Amplicor™ HIV-1 monitor v1. 5 and Nuclisens HIV-1 EasyQ® v1. 2 techniques for plasma HIV-1 RNA quantitation of non-B subtypes: the Kesho bora preparatory study
Odari et al. Establishment and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the semi-quantitative detection of HIV-1 group M virus
Goto et al. Association of plasma viral RNA load with prognosis in cats naturally infected with feline immunodeficiency virus
DK2730660T3 (da) Værktøjer og fremgangsmåde til detektering og kvantificering af genetisk forskellige HIV-1-, SIVcpz- og SIVgor-vira
Etienne et al. Single real-time reverse transcription-PCR assay for detection and quantification of genetically diverse HIV-1, SIVcpz, and SIVgor strains
De Oliveira et al. Detection of numerous HIV-1/MO recombinants in France
Ngo-Malabo et al. A cheap and open HIV viral load technique applicable in routine analysis in a resource limited setting with a wide HIV genetic diversity
Frankel et al. Development of a high-throughput multiplexed real time RT-PCR assay for detection of human pegivirus 1 and 2
Kibirige et al. Development of a sensitive, quantitative assay with broad subtype specificity for detection of total HIV-1 nucleic acids in plasma and PBMC