DK2606055T3 - Substrater til chromogen påvisning og fremgangsmåder til anvendelse i påvisningsassays og -kits - Google Patents
Substrater til chromogen påvisning og fremgangsmåder til anvendelse i påvisningsassays og -kits Download PDFInfo
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- DK2606055T3 DK2606055T3 DK11751996.7T DK11751996T DK2606055T3 DK 2606055 T3 DK2606055 T3 DK 2606055T3 DK 11751996 T DK11751996 T DK 11751996T DK 2606055 T3 DK2606055 T3 DK 2606055T3
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- nucleic acid
- compound
- detection
- target nucleic
- detection reagent
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- YVCVYCSAAZQOJI-UHFFFAOYSA-N podophyllotoxin Natural products COC1=C(O)C(OC)=CC(C2C3=CC=4OCOC=4C=C3C(O)C3C2C(OC3)=O)=C1 YVCVYCSAAZQOJI-UHFFFAOYSA-N 0.000 description 1
- 239000003600 podophyllotoxin derivative Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002953 preparative HPLC Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- FFRYUAVNPBUEIC-UHFFFAOYSA-N quinoxalin-2-ol Chemical compound C1=CC=CC2=NC(O)=CN=C21 FFRYUAVNPBUEIC-UHFFFAOYSA-N 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000021014 regulation of cell growth Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 208000000587 small cell lung carcinoma Diseases 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 125000005346 substituted cycloalkyl group Chemical group 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 125000005420 sulfonamido group Chemical group S(=O)(=O)(N*)* 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical group 0.000 description 1
- 206010042863 synovial sarcoma Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 150000003557 thiazoles Chemical class 0.000 description 1
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- 150000003585 thioureas Chemical class 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/645—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having two nitrogen atoms as the only ring hetero atoms
- C07F9/6503—Five-membered rings
- C07F9/65031—Five-membered rings having the nitrogen atoms in the positions 1 and 2
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Molecular Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Claims (17)
1. Forbindelse, som er beskrevet ved følgende formel:
herunder salte deraf; og indbefattende både R- og S-enantiomere former og ra-cemiske blandinger deraf; hvor R1 er valgt fra gruppen bestående af hydrogen, et halogenatom, en alkyl-gruppe og en arylgruppe; og hvor R2 er valgt fra gruppen bestående af hydrogen, en alkylgruppe og en arylgruppe.
2. Forbindelse ifølge krav 1, hvor halogenatomet er valgt fra gruppen bestående af chlor, fluor, brom og iod.
3. Forbindelse ifølge krav 1, hvor R1 er CFh, eller hvor R2 er phenyl.
4. Forbindelse ifølge krav 1, hvor forbindelsen er beskrevet ved følgende formel:
hvor X er to monovalente kationer eller én divalent kation.
5. Forbindelse ifølge krav 4, hvor den monovalente kation er valgt fra gruppen bestående af hydrogen, Na+, K+ eller NFl4+, eller hvor den divalente kation er Mg2+.
6. Kit, som omfatter: forbindelsen ifølge krav 1; og et diazoniumsalt.
7. Kit ifølge krav 6, hvilket kit endvidere omfatter et enzym, der katalytisk spalter forbindelsen, eventuelt hvor enzymet er en phosphatase.
8. Kit ifølge krav 7, hvor enzymet er konjugeret til et hapten eller til et antigenbindende protein eller til en nukleinsyre.
9. Kit ifølge krav 8, hvor det antigenbindende protein er et anti-hapten-antigen-bindende protein.
10. Fremgangsmåde til påvisning af et målstof i en biologisk prøve, hvilken fremgangsmåde omfatter: etablering af kontakt mellem prøven og et påvisningsreagens, som omfatter en phosphatase, der katalytisk spalter en forbindelse ifølge krav 1, hvor påvisningsreagenset direkte eller indirekte binder sig til målstoffet, og et diazoniumsalt, hvor phosphatasen spalter forbindelsen i nærværelse af diazoniumsaltet til frembringelse af en farvet forbindelse; og påvisning af tilstedeværelsen af den farvede forbindelse.
11. Kit ifølge krav 6 eller fremgangsmåde ifølge krav 10, hvor diazoniumsaltet er 4-(benzoylamino)-2,5-diethoxybenzendiazotetrachlorzincat (Fast Blue BB).
12. Fremgangsmåde ifølge krav 10, hvor målstoffet er en nukleinsyre eller et protein.
13. Fremgangsmåde ifølge krav 10, hvor påvisningsreagenset omfatter en første bindingspartner, som er konjugeret til phosphatasen.
14. Fremgangsmåde ifølge krav 13, hvor den første bindingspartner er valgt fra gruppen bestående af et antigenbindende protein, en nukleinsyre og et hapten.
15. Fremgangsmåde ifølge krav 14, hvor påvisningsreagenset omfatter en phosphatase, som er konjugeret til en nukleinsyre, målstoffet er en nukleinsyre, og påvisningsreagenset hybridiserer med målstoffet, eller hvor påvisningsreagenset omfatter en phosphatase, som er konjugeret til et antigenbindende protein, hvor målstoffet er et protein, og påvisningsreagenset binder sig til målstoffet.
16. Fremgangsmåde ifølge krav 14, hvor påvisningsreagenset indirekte binder sig til målstoffet, og påvisningsreagenset omfatter en phosphatase, som er konjugeret til et antigenbindende protein, der er specifikt for et hapten.
17. Forbindelse ifølge krav 1 eller kit ifølge krav 6 eller fremgangsmåde ifølge krav 10, hvor forbindelsen er:
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37408710P | 2010-08-16 | 2010-08-16 | |
| PCT/US2011/047613 WO2012024185A1 (en) | 2010-08-16 | 2011-08-12 | Substrates for chromogenic detection and methods of use in detection assays and kits |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2606055T3 true DK2606055T3 (da) | 2015-09-07 |
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| EP (1) | EP2606055B1 (da) |
| DK (1) | DK2606055T3 (da) |
| ES (1) | ES2542615T3 (da) |
| WO (1) | WO2012024185A1 (da) |
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| WO2013079606A1 (en) | 2011-12-01 | 2013-06-06 | Ventana Medical Systems, Inc. | Automated dual stain of mirna and protein targets |
| ES2695101T3 (es) | 2011-12-30 | 2019-01-02 | Ventana Med Syst Inc | Análisis automatizado de células tumorales circulantes |
| WO2014056812A1 (en) | 2012-10-08 | 2014-04-17 | Ventana Medical Systems, Inc. | Methods, kits, and systems for clarifying pigmented samples |
| DK3111224T3 (da) | 2014-02-24 | 2019-03-18 | Ventana Med Syst Inc | Quinonmethidanalog-signalforstærkning |
| EP3152577B1 (en) | 2014-06-06 | 2018-07-18 | Ventana Medical Systems, Inc. | Significance of intratumoral her2 heterogeneity in breast cancer and uses therefor |
| WO2016127149A2 (en) | 2015-02-06 | 2016-08-11 | Cell Idx, Inc. | Antigen-coupled immunoreagents |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE2643564C2 (de) * | 1976-09-28 | 1984-09-20 | Bayer Ag, 5090 Leverkusen | Alkoxymethyl- oder alkylthiomethylsubstituierte Pyrazolyl-(thiono) (thiol)-phosphor(phosphon)-säureester, Verfahren zu ihrer Herstellung und ihre Verwendung als Insektizide und Akarizide |
| US4711955A (en) | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
| JPS61157193A (ja) | 1984-12-28 | 1986-07-16 | Victor Co Of Japan Ltd | 映像信号再生装置 |
| US4772691A (en) | 1985-06-05 | 1988-09-20 | The Medical College Of Wisconsin, Inc. | Chemically cleavable nucleotides |
| US4888278A (en) | 1985-10-22 | 1989-12-19 | University Of Massachusetts Medical Center | In-situ hybridization to detect nucleic acid sequences in morphologically intact cells |
| US6280929B1 (en) | 1986-01-16 | 2001-08-28 | The Regents Of The University Of California | Method of detecting genetic translocations identified with chromosomal abnormalities |
| US5447841A (en) | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US5258507A (en) | 1990-11-08 | 1993-11-02 | Amoco Corporation | Labeling reagents useful for the chemical attachment of nitrophenyl derivative ligands to DNA probes |
| US5427932A (en) | 1991-04-09 | 1995-06-27 | Reagents Of The University Of California | Repeat sequence chromosome specific nucleic acid probes and methods of preparing and using |
| US6005079A (en) | 1992-08-21 | 1999-12-21 | Vrije Universiteit Brussels | Immunoglobulins devoid of light chains |
| WO1994004678A1 (en) | 1992-08-21 | 1994-03-03 | Casterman Cecile | Immunoglobulins devoid of light chains |
| US5472842A (en) | 1993-10-06 | 1995-12-05 | The Regents Of The University Of California | Detection of amplified or deleted chromosomal regions |
| US7763421B2 (en) | 2000-06-05 | 2010-07-27 | Ventana Medical Systems, Inc. | Methods for producing nucleic acid hybridization probes that amplify hybridization signal by promoting network formation |
| US6942970B2 (en) | 2000-09-14 | 2005-09-13 | Zymed Laboratories, Inc. | Identifying subjects suitable for topoisomerase II inhibitor treatment |
| CA2609702C (en) | 2005-04-28 | 2013-05-28 | Ventana Medical Systems, Inc. | Antibody conjugates via heterobifunctional peg linkers |
| CA2606018A1 (en) | 2005-04-28 | 2006-11-02 | Ventana Medical Systems, Inc. | Nanoparticle conjugates |
| CA2618377A1 (en) | 2005-08-12 | 2007-02-22 | Synta Pharmaceuticals Corp. | Pyrazole compounds that modulate hsp90 activity |
| DK2963011T3 (da) | 2005-11-23 | 2018-08-06 | Ventana Med Syst Inc | Molekylært konjugat |
| CA2661713C (en) | 2006-09-01 | 2016-08-02 | Ventana Medical Systems, Inc. | Method for producing nucleic acid probes |
| DK2444806T3 (da) | 2006-11-01 | 2014-07-21 | Ventana Med Syst Inc | Haptener, haptenkonjugater, sammensætninger deraf og fremgangsmåde til deres fremstilling og anvendelse |
| US7812140B2 (en) | 2007-04-04 | 2010-10-12 | Xerox Corporation | Colorant compounds |
| DK2167963T3 (da) | 2007-05-23 | 2019-06-24 | Ventana Med Syst Inc | Polymerbærere til immunhistokemi og in situ-hybridisering |
| CN101594302B (zh) | 2009-07-01 | 2011-08-03 | 华为技术有限公司 | 数据出队的方法及装置 |
| US20120070862A1 (en) | 2009-12-31 | 2012-03-22 | Ventana Medical Systems, Inc. | Methods for producing uniquely distinct nucleic acid tags |
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- 2011-08-12 WO PCT/US2011/047613 patent/WO2012024185A1/en active Application Filing
- 2011-08-12 EP EP11751996.7A patent/EP2606055B1/en not_active Not-in-force
- 2011-08-12 DK DK11751996.7T patent/DK2606055T3/da active
- 2011-08-12 US US13/809,354 patent/US9127028B2/en not_active Expired - Fee Related
- 2011-08-12 ES ES11751996.7T patent/ES2542615T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US9127028B2 (en) | 2015-09-08 |
| EP2606055B1 (en) | 2015-06-03 |
| WO2012024185A1 (en) | 2012-02-23 |
| ES2542615T3 (es) | 2015-08-07 |
| US20130115593A1 (en) | 2013-05-09 |
| EP2606055A1 (en) | 2013-06-26 |
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