DK2547763T3 - Generering af klonale mesenchymprogenitorer og mesenchymstamcellelinjer - Google Patents

Generering af klonale mesenchymprogenitorer og mesenchymstamcellelinjer Download PDF

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DK2547763T3
DK2547763T3 DK11710630.2T DK11710630T DK2547763T3 DK 2547763 T3 DK2547763 T3 DK 2547763T3 DK 11710630 T DK11710630 T DK 11710630T DK 2547763 T3 DK2547763 T3 DK 2547763T3
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cells
mesenchymal
culture
cell
colonies
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Maksym A Vodyanyk
Igor I Slukvin
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Wisconsin Alumni Res Found
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Claims (9)

1. Fremgangsmåde til generering af en population af primat-mesodermceller, der udtrykker Apelin-receptor, hvilken fremgangsmåde omfatter følgende trin; dyrkning af isolerede pluripotente primat-stamceller i samdyrkning med OP9-knoglemarvsstromaceller, indtil Apelin-receptor udtrykkes og kan detekteres i de samdyrkede celler; og sortering af de samdyrkede celler, der udtrykker Apelin-receptor, fra celler af samkulturen, der ikke udtrykker Apelin-receptor.
2. Fremgangsmåde ifølge krav 1, hvor de isolerede pluripotente primatstamceller samdyrkes med OP9-knoglemarvsstromaceller i ca. to til ca. tre dage.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor ca. 1 % til ca. 5 % af Apelin-receptor+-cellerne er i stand til at differentiere i dyrkning til mesenchymstamceller og endotelceller.
4. Fremgangsmåde til generering af en population af mesangioblaster, der er fælles mesenchym- og endotelcellepræcursorer, hvilken fremgangsmåde omfatter følgende trin; dyrkning af isolerede pluripotente primat-stamceller i samkultur med OP9-knoglemarvsstomaceller, indtil Apelin-receptor udtrykkes og kan detekteres i de samdyrkede celler; sortering af de samdyrkede celler, der udtrykker Apelin-receptor, fra celler af samkulturen, der ikke udtrykker Apelin-receptor; og dyrkning af Apelin receptor+-celler i et semifast dyrkningsmedium, der omfatter bFGF, indtil et undersæt differentierer for at danne mesangioblastkolonier.
5. Fremgangsmåde ifølge krav 4, der endvidere omfatter dyrkning af mesangioblasterne i et dyrkningsmedium, der omfatter bFGF, indtil mesangioblasterne differentierer til endotelceller.
6. Fremgangsmåde ifølge krav 4, der endvidere omfatter følgende trin: dyrkning af mesangioblasterne i tilstedeværelse af bFGF, indtil mesangioblasterne differentierer til mesenkymale stamceller.
7. Isoleret population af primat-mesangioblaster med endotel- og mesenchympotentiale opnået ved fremgangsmåden ifølge krav 4.
8. Fremgangsmåde ifølge krav 4, hvor mesangioblasterne dannes på ca. to til ca. tre dages dyrkning i det semifaste medium.
9. Fremgangsmåde ifølge krav 5, hvor det semifaste dyrkningsmedium endvidere omfatter knoglemorfogent protein 4 (BMP4).
DK11710630.2T 2010-03-18 2011-03-16 Generering af klonale mesenchymprogenitorer og mesenchymstamcellelinjer DK2547763T3 (da)

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US12/726,814 US20110236971A2 (en) 2007-09-25 2010-03-18 Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
PCT/US2011/028700 WO2011116117A2 (en) 2010-03-18 2011-03-16 Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions

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EP (1) EP2547763B1 (da)
JP (1) JP5944885B2 (da)
AU (1) AU2011227274B2 (da)
BR (1) BR112012023537B1 (da)
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Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2137296A2 (en) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
US20110236971A2 (en) * 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
EA034058B1 (ru) * 2011-01-12 2019-12-23 Цунео Кидо Способ культивирования для получения и поддержания чистой или обогащенной популяции нервных стволовых клеток и/или нервных клеток-предшественников млекопитающего, склонных к дифференцировке с образованием клеток олигодендроцитарной линии in vitro
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
AU2012298997B2 (en) 2011-08-23 2018-10-04 Wisconsin Alumni Research Foundation Angiohematopoietic progenitor cells
US20140349396A1 (en) * 2011-09-09 2014-11-27 Michael West Compositions and Methods Relating to Clonal Progenitor Cells
US8961956B2 (en) 2011-11-30 2015-02-24 Ocata Therapeutics, Inc. Mesenchymal stromal cells and uses related thereto
CN108478599B (zh) 2011-11-30 2022-08-02 安斯泰来再生医药协会 间充质基质细胞及其相关用途
EP2804945A1 (en) * 2012-01-19 2014-11-26 UCL Business PLC Method for obtaining mab-like cells and uses thereof
CA2876499C (en) 2012-07-12 2021-10-26 Imstem Biotechnology, Inc. Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
CA3141731A1 (en) * 2013-03-13 2014-10-09 Wisconsin Alumni Research Foundation Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
WO2014146057A2 (en) * 2013-03-15 2014-09-18 Cmr Technologies, Llc Mesenchymal stem cells
US9868939B2 (en) 2013-06-12 2018-01-16 Wisconsin Alumni Research Foundation Generating vasculogenic cell populations
KR102250027B1 (ko) * 2013-07-26 2021-05-07 교토후고리츠다이가쿠호진 골아 세포 및 그 조제 방법
WO2016139340A1 (en) * 2015-03-04 2016-09-09 Mesoblast International Sàrl Cell culture method for mesenchymal stem cells
US10704024B2 (en) 2015-08-21 2020-07-07 Adiposeeds, Inc. Method for producing mesenchymal cells with promoted c-MPL receptor expression on cell surface
CN105754936B (zh) * 2016-02-25 2018-06-08 付清玲 人诱导性多能干细胞诱导为间充质干细胞的方法
JP7108537B2 (ja) * 2016-04-27 2022-07-28 ロート製薬株式会社 Ｃｄ２０１、ｃｄ４６、ｃｄ５６、ｃｄ１４７及びｃｄ１６５からなる群より選択される少なくとも１種の細胞表面マーカーを発現する間葉系幹細胞及びその調製方法、並びに上記間葉系幹細胞を含む医薬組成物及びその調製方法
WO2018009147A1 (en) * 2016-07-04 2018-01-11 Agency For Science, Technology And Research Method of generating mesenchymal stem cells and uses thereof
AU2017308738B2 (en) * 2016-08-10 2023-09-21 Indiana University Research And Technology Corporation Method for generating mesoderm and/or endothelial colony forming cell-like cells having in vivo blood vessel forming capacity
WO2019226588A1 (en) * 2018-05-23 2019-11-28 University Of Florida Research Foundation Paramagnetic immunobeads for the isolation of human adipose-derived stem cells
US20220143099A1 (en) * 2019-04-02 2022-05-12 The General Hospital Corporation Methods to enhance t cell regeneration
WO2022076751A1 (en) * 2020-10-07 2022-04-14 The Regents Of The University Of Colorado, A Body Corporate Compositions, methods and use for in vitro and in vivo generation of functional thymic tissue
CN112608893B (zh) * 2020-12-25 2023-03-21 博雅干细胞科技有限公司 胎盘间充质干细胞的分离和纯化方法
WO2022196714A1 (ja) 2021-03-17 2022-09-22 アステラス製薬株式会社 塩基性繊維芽細胞増殖因子（ｂＦＧＦ）遺伝子が導入されたペリサイト
JPWO2023286834A1 (da) 2021-07-15 2023-01-19
JPWO2023286832A1 (da) 2021-07-15 2023-01-19
CN114752688B (zh) * 2022-04-28 2024-02-23 北京大学口腔医学院 鉴定人类胚胎骨髓来源的间充质干细胞的方法、探针和试剂盒及其用途

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6602711B1 (en) 2000-02-21 2003-08-05 Wisconsin Alumni Research Foundation Method of making embryoid bodies from primate embryonic stem cells
JP2005123904A (ja) * 2003-10-16 2005-05-12 Nippon Dempa Kogyo Co Ltd 表面実装用の水晶発振器
WO2005111197A1 (en) 2004-05-07 2005-11-24 Wisconsin Alumni Research Foundation Method of forming mesenchymal stem cells from embryonic stem cells
US20080025955A1 (en) 2004-06-22 2008-01-31 Mitsubishi Tanabe Pharma Corporation Method Of Producing Vascular Endothelial Cells From Primate Embryonic Stem Cells
EP1937801A1 (en) * 2005-09-02 2008-07-02 Agency for Science, Technology and Research Method of deriving mesenchymal stem cells
EP2617428A1 (en) * 2006-08-15 2013-07-24 Agency for Science, Technology and Research Mesenchymal stem cell conditioned medium
EP2137296A2 (en) * 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
US20110236971A2 (en) * 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
US7615374B2 (en) * 2007-09-25 2009-11-10 Wisconsin Alumni Research Foundation Generation of clonal mesenchymal progenitors and mesenchymal stem cell lines under serum-free conditions

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CA2793380A1 (en) 2011-09-22
AU2011227274A1 (en) 2012-10-04
US20110236971A2 (en) 2011-09-29
BR112012023537A2 (pt) 2017-05-23
JP2013521806A (ja) 2013-06-13
EP2547763B1 (en) 2018-08-22
MX2012010721A (es) 2012-10-05
JP5944885B2 (ja) 2016-07-05
US20100261274A1 (en) 2010-10-14
US9771561B2 (en) 2017-09-26
MX337484B (es) 2016-03-08
EP2547763A2 (en) 2013-01-23
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