DK2478137T3 - Reduceret kodonmutagenese - Google Patents

Reduceret kodonmutagenese

Info

Publication number
DK2478137T3
DK2478137T3 DK10817881.5T DK10817881T DK2478137T3 DK 2478137 T3 DK2478137 T3 DK 2478137T3 DK 10817881 T DK10817881 T DK 10817881T DK 2478137 T3 DK2478137 T3 DK 2478137T3
Authority
DK
Denmark
Prior art keywords
mutagenese
reduced codon
codon
reduced
codon mutagenese
Prior art date
Application number
DK10817881.5T
Other languages
English (en)
Inventor
Richard Fox
Lorraine J Giver
Daniel Held
Douglas Hattendorf
Trish Choudhary
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/562,988 external-priority patent/US8383346B2/en
Priority claimed from PCT/US2009/057507 external-priority patent/WO2010144103A1/en
Application filed by Codexis Inc filed Critical Codexis Inc
Application granted granted Critical
Publication of DK2478137T3 publication Critical patent/DK2478137T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK10817881.5T 2009-09-18 2010-09-17 Reduceret kodonmutagenese DK2478137T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12/562,988 US8383346B2 (en) 2008-06-13 2009-09-18 Combined automated parallel synthesis of polynucleotide variants
PCT/US2009/057507 WO2010144103A1 (en) 2009-06-11 2009-09-18 Combined automated parallel synthesis of polynucleotide variants
US28387709P 2009-12-09 2009-12-09
PCT/US2010/049251 WO2011035105A1 (en) 2009-09-18 2010-09-17 Reduced codon mutagenesis

Publications (1)

Publication Number Publication Date
DK2478137T3 true DK2478137T3 (da) 2017-11-27

Family

ID=43759019

Family Applications (2)

Application Number Title Priority Date Filing Date
DK17190712.4T DK3301208T3 (da) 2009-09-18 2010-09-17 Reduceret codon-mutagenese
DK10817881.5T DK2478137T3 (da) 2009-09-18 2010-09-17 Reduceret kodonmutagenese

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK17190712.4T DK3301208T3 (da) 2009-09-18 2010-09-17 Reduceret codon-mutagenese

Country Status (5)

Country Link
EP (2) EP2478137B1 (da)
DK (2) DK3301208T3 (da)
HU (2) HUE051913T2 (da)
SG (1) SG179197A1 (da)
WO (1) WO2011035105A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL263448B2 (en) 2016-06-09 2023-10-01 Codexis Inc Biological catalysts and methods for hydroxylation of chemical compounds
BR112018075932A2 (pt) 2016-06-15 2019-10-01 Codexis Inc variante de beta-glicosidase de ocorrência não natural, polinucleotídeo recombinante, vetor, célula hospedeira, métodos para produção de pelo menos uma variante de beta-glicosidade de ocorrência não natural, para conversão de um substrato aceptor de grupo glicosila em um produto beta-glicosilado e para transglicosilação de um substrato, e, composição.
IL264686B2 (en) 2016-08-26 2024-07-01 Codexis Inc Engineered imine reductases and methods for reversible amination of ketone and amine compounds
CN114901820B (zh) * 2019-12-30 2024-01-19 南京金斯瑞生物科技有限公司 构建基因突变文库的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
FI104465B (fi) 1995-06-14 2000-02-15 Valio Oy Proteiinihydrolysaatteja allergioiden hoitamiseksi tai estämiseksi, niiden valmistus ja käyttö
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
JP4221100B2 (ja) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 半導体装置
EP1130093A1 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
WO2001075767A2 (en) 2000-03-30 2001-10-11 Maxygen, Inc. In silico cross-over site selection
DE60144145D1 (de) 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
BRPI0413501A (pt) 2003-08-11 2006-10-10 Codexis Inc polipetìdeo, polinucleotìdeo isolado ou recombinante, vetor de expressão, célula hospedeira, e, método de preparar um polipetìdeo de hhdh
EP1654354A1 (en) 2003-08-11 2006-05-10 Codexis, Inc. Improved ketoreductase polypeptides and related polynucleotides
BRPI0619225A2 (pt) * 2005-12-01 2017-11-07 Domantis Ltd monômero de anticorpo de domínio, ligando, ácidos nucleicos isolado e recombinante, vetor, célula hospedeira, método para produzir um monômero de dab de ligando, composição farmacêutica, dispositivo de liberação de medicamento, uso de um monômero de anticorpo de domínio, e, método para tratar uma doença inflamatória, artrite ou doença respiratória
WO2008103474A1 (en) * 2007-02-20 2008-08-28 Anaptysbio, Inc. Methods of generating libraries and uses thereof
WO2009088928A2 (en) * 2007-12-31 2009-07-16 Xoma Technology Ltd. Libraries, arrays and their uses for targeted affinity enhancement
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants

Also Published As

Publication number Publication date
HUE037123T2 (hu) 2018-08-28
EP2478137A4 (en) 2013-10-23
WO2011035105A1 (en) 2011-03-24
EP3301208A1 (en) 2018-04-04
EP2478137A1 (en) 2012-07-25
HUE051913T2 (hu) 2021-04-28
DK3301208T3 (da) 2020-12-14
EP2478137B1 (en) 2017-09-13
SG179197A1 (en) 2012-04-27
EP3301208B1 (en) 2020-11-04

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