DK2454373T3 - Forhøjet celleproduktion ved mechano-transduktion - Google Patents

Forhøjet celleproduktion ved mechano-transduktion Download PDF

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Publication number
DK2454373T3
DK2454373T3 DK10740787.6T DK10740787T DK2454373T3 DK 2454373 T3 DK2454373 T3 DK 2454373T3 DK 10740787 T DK10740787 T DK 10740787T DK 2454373 T3 DK2454373 T3 DK 2454373T3
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DK
Denmark
Prior art keywords
cells
cell
antibody
certain embodiments
tensegrity
Prior art date
Application number
DK10740787.6T
Other languages
English (en)
Inventor
Reema Piparia
Ivan Correia
Original Assignee
Abbvie Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbvie Inc filed Critical Abbvie Inc
Application granted granted Critical
Publication of DK2454373T3 publication Critical patent/DK2454373T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06—Magnetic means

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (10)

1. Fremgangsmåde til forøgelse af produktionen af antistof af interesse fra en værtscelle omfattende at udsætte nævnte celle for en tensegritetskraft, hvor tensegritetskraften tilføres cellen i en mængde, der er effektiv til at forøge nævnte antistof-produktion fra cellen.
2. Fremgangsmåde ifølge krav 1, hvor tensegritetskraften er en mekanisk belastning med høj forskydningsspænding.
3. Fremgangsmåde ifølge krav 1, hvor cellen er hæftet på et substrat, og tensegritetskraften er en biaksial spænding.
4. Fremgangsmåde ifølge krav 1, hvor cellen er en Chinese hamster ovary-celle (CHO).
5. Fremgangsmåde ifølge krav 1, hvor antistoffet er ABT-874.
6. Fremgangsmåde ifølge krav 1, hvor cellen er dyrket i en bioreaktor.
7. Fremgangsmåde ifølge krav 7, hvor cellen er hæftet på et substrat.
8. Fremgangsmåde ifølge krav 1, hvor tensegritetskraften er cyklisk lydtryk.
9. Fremgangsmåde ifølge krav 8, hvor det cykliske lydtryk er ultralyd.
10. Fremgangsmåde ifølge krav 2, hvor cellen bringes i kontakt med en ferro-magnetisk mikroperle, og hvor en magnetisk kraft påføres den ferromagnetiske mikroperle.
DK10740787.6T 2009-07-15 2010-07-15 Forhøjet celleproduktion ved mechano-transduktion DK2454373T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22569409P 2009-07-15 2009-07-15
PCT/US2010/042143 WO2011008959A1 (en) 2009-07-15 2010-07-15 Enhancement of cellular production through mechanotransduction

Publications (1)

Publication Number Publication Date
DK2454373T3 true DK2454373T3 (da) 2015-02-23

Family

ID=42702283

Family Applications (1)

Application Number Title Priority Date Filing Date
DK10740787.6T DK2454373T3 (da) 2009-07-15 2010-07-15 Forhøjet celleproduktion ved mechano-transduktion

Country Status (10)

Country Link
US (1) US20110117603A1 (da)
EP (1) EP2454373B1 (da)
JP (1) JP2012533306A (da)
CN (1) CN102471775A (da)
CA (1) CA2766842A1 (da)
DK (1) DK2454373T3 (da)
ES (1) ES2528246T3 (da)
MX (1) MX2012000705A (da)
SI (1) SI2454373T1 (da)
WO (1) WO2011008959A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2780445A4 (en) * 2011-11-16 2015-07-15 Harvard College DEFORMABLE PLATFORMS FOR BIOLOGICAL ASSAYS
US10640742B2 (en) 2014-09-25 2020-05-05 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Hybrid linear actuator controlled hydraulic cell stretching
IL311774A (en) * 2021-10-01 2024-05-01 Regeneron Pharma Methods for quantifying the effect of shear stress on mammalian cell lines

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE30985E (en) * 1978-01-01 1982-06-29 Serum-free cell culture media
US4634665A (en) * 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4399216A (en) * 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US5179017A (en) 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4510245A (en) * 1982-11-18 1985-04-09 Chiron Corporation Adenovirus promoter system
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) * 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US5168062A (en) 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
US4968615A (en) * 1985-12-18 1990-11-06 Ciba-Geigy Corporation Deoxyribonucleic acid segment from a virus
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
AU632065B2 (en) 1988-09-23 1992-12-17 Novartis Vaccines And Diagnostics, Inc. Cell culture medium for enhanced cell growth, culture longevity and product expression
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
EP0402226A1 (en) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
WO1998045468A1 (en) * 1997-04-08 1998-10-15 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration (Nasa) Production of functional proteins: balance of shear stress and gravity
US6914128B1 (en) * 1999-03-25 2005-07-05 Abbott Gmbh & Co. Kg Human antibodies that bind human IL-12 and methods for producing

Also Published As

Publication number Publication date
MX2012000705A (es) 2012-03-07
WO2011008959A1 (en) 2011-01-20
CN102471775A (zh) 2012-05-23
SI2454373T1 (sl) 2015-03-31
EP2454373A1 (en) 2012-05-23
CA2766842A1 (en) 2011-01-20
EP2454373B1 (en) 2014-11-19
JP2012533306A (ja) 2012-12-27
ES2528246T3 (es) 2015-02-05
US20110117603A1 (en) 2011-05-19

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