DK2449098T3 - Oprensning og isolering af rekombinante oxalat-nedbrydende enzymer og sprøjtetørrede partikler indeholdende oxalat-nedbrydende enzymer - Google Patents

Oprensning og isolering af rekombinante oxalat-nedbrydende enzymer og sprøjtetørrede partikler indeholdende oxalat-nedbrydende enzymer Download PDF

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DK2449098T3
DK2449098T3 DK10744865.6T DK10744865T DK2449098T3 DK 2449098 T3 DK2449098 T3 DK 2449098T3 DK 10744865 T DK10744865 T DK 10744865T DK 2449098 T3 DK2449098 T3 DK 2449098T3
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oxalate
seq
oxdc
particles
enzyme
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DK10744865.6T
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English (en)
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Harmeet Sidhu
Qingshan Li
Aaron Blake Cowley
Carl-Gustaf Gölander
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Oxthera Intellectual Property Ab
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Priority claimed from EP09164430.2A external-priority patent/EP2270138B1/en
Priority claimed from US12/497,275 external-priority patent/US8431122B2/en
Application filed by Oxthera Intellectual Property Ab filed Critical Oxthera Intellectual Property Ab
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Publication of DK2449098T3 publication Critical patent/DK2449098T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/04—Drugs for disorders of the urinary system for urolithiasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
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  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Hematology (AREA)
  • Cardiology (AREA)
  • Pain & Pain Management (AREA)
  • Diabetes (AREA)
  • Rheumatology (AREA)
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  • Enzymes And Modification Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (10)

1. Sprøjtetørrede partikler omfattende et eller flere rekombinante proteiner valgt fra gruppen bestående af C383S- og C383A-mutanten af det rekombinante oxalat-decarboxylase-(OxDC)-vildtype-protein i henhold til SEQ. ID NO. 1 eller SEQ. ID NO. 2, eller et protein kodet af en sekvens valgt fra SEQ. ID NO. 3, SEQ. ID NO. 4, SEQ. ID NO. 5, SEQ. ID NO. 6, SEQ. ID NO. 7, SEQ. ID NO. 8, SEQ. ID NO. 15, SEQ. ID NO. 16, SEQ. ID NO. 17, SEQ. ID NO. 18, eller SEQ. ID NO. 19; og et polymermateriale, hvor proteinerne er frembragt som nano- eller mikroagglomerater.
2. Sprøjtetørrede partikler ifølge krav 1, hvor det oxalat-reducerende enzym er C383S-mutanten af det rekombinante OxDC-vildtype-protein eller et protein kodet af en sekvens valgt fra SEQ. ID NO. 3, SEQ. ID NO. 4, SEQ. ID NO. 5, SEQ. ID NO. 6, SEQ. ID NO. 7 eller SEQ. ID NO. 8.
3. Sprøjtetørrede partikler ifølge krav 1, hvor det oxalat-reducerende enzym er C383A-mutanten af det rekombinante OxDC-vildtype-protein eller et protein kodet afen sekvens valgt fra SEQ. ID NO. 15, SEQ. ID NO. 16, SEQ. ID NO. 17, SEQ. ID NO. 18 eller SEQ. ID NO. 19.
4. Sprøjtetørrede partikler ifølge et hvilket som helst af kravene 1-3, hvor aktiviteten af det ene eller flere oxalat-reducerende enzymer når til stede i partiklerne højst falder til omkring 30% når inkuberet i en 3,2 mg/ml pepsinopløsning med en pH på omkring 3,2 i 40 minutter, hvor den initiale aktivitet sættes til 100%.
5. Sprøjtetørrede partikler ifølge et hvilket som helst af kravene 1-4, hvor polymermaterialet er et poly(meth)akrylat.
6. Sammensætning omfattende sprøjtetørrede partikler som defineret i et hvilket som helst af kravene 1-5.
7. Sammensætningen ifølge krav 6, hvor sammensætningen er en oral dosisform.
8. Sammensætningen ifølge krav 6 eller 7 i formen af en lille pose, tablet, kapsel, tyggetablet, hurtigopløsende tablet, oralt desintegrerende tablet, væsker, sirupper, eller eliksirer eller andet leveringsformat.
9. Sammensætningen ifølge et hvilket som helst af kravene 6-8 til anvendelse i en fremgangsmåde til behandling af et menneskeligt eller dyreindivid ved at reducere oxalat til stede i maven for derved at reducere absorptionen af oxalatet, hvilken fremgangsmåde omfatter oral administration af sammensætningen til maven af det menneskelige eller dyreindivid.
10. Sammensætningen til anvendelse ifølge krav 9, hvor reduktion af absorption af oxalat tilvejebringer behandling af en oxalat-relateret tilstand valgt fra gruppen bestående af hyperoxaluri, absorptiv hyperoxaluri, enterisk hyperoxaluri, primær hyperoxaluri, idiopatisk calciumoxalat-nyrestenssygdom, urolithiasis, vulvodyni, oxalose associeret med slutstadie nyresygdom, hjertekonduktansforstyrrelse, inflammatorisk tarmsygdom, Crohns sygdom, ulcerøs colitis, postoperative gastrointestinale tilstande, og postoperative bariatriske tilstande, postoperative fedme tilstande, eller postantibiotika behandling.
DK10744865.6T 2009-07-02 2010-07-01 Oprensning og isolering af rekombinante oxalat-nedbrydende enzymer og sprøjtetørrede partikler indeholdende oxalat-nedbrydende enzymer DK2449098T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09164430.2A EP2270138B1 (en) 2009-07-02 2009-07-02 Purification and isolation of recombinant oxalate degrading enzymes
US12/497,275 US8431122B2 (en) 2009-07-02 2009-07-02 Purification and isolation of recombinant oxalate degrading enzymes and spray-dried particles containing oxalate degrading enzymes
PCT/EP2010/003864 WO2011000523A1 (en) 2009-07-02 2010-07-01 Purification and isolation of recombinant oxalate degrading enzymes and spray-dried particles containing oxalate degrading enzymes

Publications (1)

Publication Number Publication Date
DK2449098T3 true DK2449098T3 (da) 2017-03-06

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DK10744865.6T DK2449098T3 (da) 2009-07-02 2010-07-01 Oprensning og isolering af rekombinante oxalat-nedbrydende enzymer og sprøjtetørrede partikler indeholdende oxalat-nedbrydende enzymer

Country Status (16)

Country Link
US (1) US20120189604A1 (da)
EP (1) EP2449098B1 (da)
JP (2) JP2012531186A (da)
CN (2) CN102597225B (da)
BR (1) BRPI1013956B8 (da)
CA (2) CA2767039C (da)
DK (1) DK2449098T3 (da)
ES (1) ES2617917T3 (da)
HK (1) HK1210805A1 (da)
HU (1) HUE030905T2 (da)
IL (1) IL216873A (da)
IN (1) IN2012DN00592A (da)
LT (1) LT2449098T (da)
PL (1) PL2449098T3 (da)
PT (1) PT2449098T (da)
WO (1) WO2011000523A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8431122B2 (en) 2009-07-02 2013-04-30 Oxthera Intellectual Property Ab Purification and isolation of recombinant oxalate degrading enzymes and spray-dried particles containing oxalate degrading enzymes
US9714456B2 (en) * 2013-01-18 2017-07-25 Allena Pharmaceuticals, Inc. Crystallized oxalate decarboxylase and methods of use
CN103540569A (zh) * 2013-06-05 2014-01-29 黄虹寓 结石酶及其制备方法
EP3003358B1 (en) * 2013-06-07 2021-03-24 Allena Pharmaceuticals Inc. Compositions and devices for dialysis
CN107254453B (zh) * 2014-12-22 2019-10-25 武汉康复得生物科技股份有限公司 一种在生理pH条件下有活力的草酸氧化酶及其应用
CN116124853B (zh) * 2022-11-07 2024-01-16 东北农业大学 一种检测草酸的电化学生物传感器、制备方法及应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8486389B2 (en) * 1997-05-23 2013-07-16 Oxthera, Inc. Compositions and methods for treating or preventing oxalate-related disease
WO2004018634A2 (en) * 2002-08-20 2004-03-04 University Of Florida Polynucleotide encoding oxalate decarboxylase from aspergillus niger and methods of use
US6929940B1 (en) * 2002-08-20 2005-08-16 University Of Florida Research Foundation, Inc. Polynucleotides encoding oxalate decarboxylase from Aspergillus niger and methods of use
PT1962873E (pt) * 2005-12-14 2013-08-29 Oxthera Inc Composições farmacêuticas compreendendo bactérias redutoras de oxalato
ES2362897T3 (es) * 2005-12-16 2011-07-14 Oxthera, Inc. Composiciones y métodos para reducción del oxalato.
WO2008105911A2 (en) * 2006-08-02 2008-09-04 Altus Pharmaceuticals Inc. Crystallized oxalate decarboxylase and methods of use

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Publication number Publication date
US20120189604A1 (en) 2012-07-26
IN2012DN00592A (da) 2015-06-12
CN104805067A (zh) 2015-07-29
JP6220845B2 (ja) 2017-10-25
CN102597225B (zh) 2015-04-29
CN102597225A (zh) 2012-07-18
HUE030905T2 (en) 2017-06-28
IL216873A (en) 2017-07-31
CA2767039A1 (en) 2011-01-06
BRPI1013956B1 (pt) 2021-04-06
PT2449098T (pt) 2017-03-10
EP2449098B1 (en) 2016-12-07
LT2449098T (lt) 2017-05-25
PL2449098T3 (pl) 2017-06-30
BRPI1013956A2 (pt) 2019-04-09
EP2449098A1 (en) 2012-05-09
CA3012811A1 (en) 2011-01-06
BRPI1013956B8 (pt) 2021-05-25
ES2617917T3 (es) 2017-06-20
JP2016121138A (ja) 2016-07-07
IL216873A0 (en) 2012-02-29
CA2767039C (en) 2018-09-04
JP2012531186A (ja) 2012-12-10
HK1210805A1 (en) 2016-05-06
WO2011000523A1 (en) 2011-01-06

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