DK2426220T3 - Mærkede mikroorganismer og fremgangsmåder til mærkning - Google Patents

Mærkede mikroorganismer og fremgangsmåder til mærkning Download PDF

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Publication number
DK2426220T3
DK2426220T3 DK11161778.3T DK11161778T DK2426220T3 DK 2426220 T3 DK2426220 T3 DK 2426220T3 DK 11161778 T DK11161778 T DK 11161778T DK 2426220 T3 DK2426220 T3 DK 2426220T3
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Denmark
Prior art keywords
crispr
sequence
bacterium
bacteriophage
spacer
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DK11161778.3T
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English (en)
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Rodolphe Barrangou
Christophe Fremaux
Philippe Horvath
Dennis Romero
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Dupont Nutrition Biosci Aps
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (18)

1. Fremgangsmåde til fremstilling af en fødevarebestanddel, der omfatter opnåelse af en mærket bakterie ved hjælp af en fremgangsmåde, der omfatter trinene: (a) eksponering af en moderbakterie, der omfatter et CRISPR-locus, for en bakteriofag til indførelse af en yderligere gentagelse-spacer-enhed i CRISPR-locuset til frembringelse af en mærket bakterie, hvor den yderligere gentagelse-spacer-enhed tilvejebringer mærket, (b) selektion af en bakteriofag-ufølsom mutant; (c) sammenligning af et CRISPR-locus eller en del deraf fra moderbakterien og den bakteriofag-ufølsomme mutant; (d) selektion af en mærket bakterie, der omfatter den yderligere gentagelse-spacer-enhed i CRISPR-locuset, der ikke er til stede i moderbakterien; og yderligere omfatter trinet tilsætning af den mærkede bakterie eller en cellekultur, der omfatter den mærkede bakterie, til fødevarebestanddelen.
2. Fremgangsmåde ifølge krav 1, hvor moderbakterien er udvalgt fra gruppen af slægter, der består af: Bacillus, Agrobacterium, Staphylococcus, Streptococcus, Enterococcus, Corynebacterium, Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Lactobacillus, Pediococcus, Leuconostoc og Oenococcus.
3. Fremgangsmåde ifølge krav 1 eller krav 2, hvilken fremgangsmåde omfatter eksponering af moderbakterien for to eller flere bakteriofager enten samtidigt eller sekventielt, og hvor der selekteres en mærket bakterie, der omfatter to eller tre eller flere yderligere gentagelse-spacer-enheder.
4. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor den mærkede bakterie akkumulerer de yderligere gentagelse-spacer-enheder i mere end ét CRISPR-locus.
5. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor moderbakterien er Streptococcus.
6. Fremgangsmåde ifølge krav 5, hvor moderbakterien er Streptococcus thermophilus.
7. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor bakteriofagen er udvalgt fra gruppen af virusfamilier, der består af: Corticoviridae, Cystoviridae, Inoviridae, Leviviridae, Microviridae, Myoviridae, Podoviridae, Siphoviridae og Tectiviridae.
8. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor bakteriof agen er i stand til at inficere moderbakterien.
9. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor en cellekultur, der omfatter den mærkede bakterie, tilsættes til fødevarebestanddelen.
10. Fremgangsmåde ifølge krav 9, hvor cellekulturen er en starterkultur, en probiotikumkultur eller et kosttilskud.
11. Fremgangsmåde ifølge krav 10, hvor cellekulturen er en starterkultur.
12. Fremgangsmåde ifølge krav 11, hvor starterkulturen er en frossen kultur.
13. Fremgangsmåde ifølge krav 11, hvor starterkulturen er en tørret starterkultur.
14. Fremgangsmåde ifølge krav 13, hvor den tørrede starterkultur er frysetørret.
15. Fremgangsmåde ifølge krav 11, hvor starterkulturen er en defineret blandet kultur.
16. Fremgangsmåde ifølge krav 11, hvor starterkulturen er en koncentreret starterkulturanvendelse til direkte podning.
17. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor fødevarebestanddelen er egnet til tilsætning til en fødevare, og hvor fødevaren er udvalgt fra gruppen, der består af konfektureprodukter, mejeriprodukter, kødprodukter, fjerkræprodukter, fiskeprodukter og bageriprodukter.
18. Fremgangsmåde ifølge krav 17, hvor fødevaren er udvalgt fra gruppen, der består af alkoholfri drikke, frugtjuicer, drikke, der omfatter valleprotein, sundhedsteer, kakaodrikke, mælkedrikke, mælkesyrebakteriedrikke, ost, yoghurt, drikkeyoghurt og vin.
DK11161778.3T 2006-05-19 2007-05-18 Mærkede mikroorganismer og fremgangsmåder til mærkning DK2426220T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74768206P 2006-05-19 2006-05-19
US90472107P 2007-03-02 2007-03-02
EP07777180A EP2018441B1 (en) 2006-05-19 2007-05-18 Tagged microorganisms and methods of tagging

Publications (1)

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DK2426220T3 true DK2426220T3 (da) 2016-09-26

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DK11161778.3T DK2426220T3 (da) 2006-05-19 2007-05-18 Mærkede mikroorganismer og fremgangsmåder til mærkning
DK07777180.6T DK2018441T3 (da) 2006-05-19 2007-05-18 Mærkede mikroorganismer og fremgangsmåde til mærkning

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US (2) US9399801B2 (da)
EP (2) EP2426220B1 (da)
AT (1) ATE530669T1 (da)
DK (2) DK2426220T3 (da)
ES (2) ES2373586T3 (da)
PL (2) PL2018441T3 (da)
WO (1) WO2007136815A2 (da)

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Also Published As

Publication number Publication date
ES2590925T3 (es) 2016-11-24
US9816140B2 (en) 2017-11-14
WO2007136815A3 (en) 2008-01-31
ATE530669T1 (de) 2011-11-15
EP2018441A2 (en) 2009-01-28
WO2007136815A2 (en) 2007-11-29
US20100104690A1 (en) 2010-04-29
US20080124725A1 (en) 2008-05-29
DK2018441T3 (da) 2012-01-02
PL2018441T3 (pl) 2012-03-30
US9399801B2 (en) 2016-07-26
PL2426220T3 (pl) 2017-01-31
EP2018441B1 (en) 2011-10-26
EP2426220A1 (en) 2012-03-07
ES2373586T3 (es) 2012-02-06
EP2426220B1 (en) 2016-06-22

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