DK186589D0 - E. COLI STEMS FOR POWERFUL SECRETION OF PROTEIN FOR THE CULTURAL MEDIUM, ITS MANUFACTURING AND USE - Google Patents

E. COLI STEMS FOR POWERFUL SECRETION OF PROTEIN FOR THE CULTURAL MEDIUM, ITS MANUFACTURING AND USE

Info

Publication number
DK186589D0
DK186589D0 DK186589A DK186589A DK186589D0 DK 186589 D0 DK186589 D0 DK 186589D0 DK 186589 A DK186589 A DK 186589A DK 186589 A DK186589 A DK 186589A DK 186589 D0 DK186589 D0 DK 186589D0
Authority
DK
Denmark
Prior art keywords
secretion
stems
coli
powerful
protein
Prior art date
Application number
DK186589A
Other languages
Danish (da)
Other versions
DK186589A (en
DK174508B1 (en
Inventor
August Boeck
Wiebke Seydel
Gerhard Schmid
Gabriele Mueller
Original Assignee
Consortium Elektrochem Ind
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consortium Elektrochem Ind filed Critical Consortium Elektrochem Ind
Publication of DK186589D0 publication Critical patent/DK186589D0/en
Publication of DK186589A publication Critical patent/DK186589A/en
Application granted granted Critical
Publication of DK174508B1 publication Critical patent/DK174508B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Led Devices (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to Escherichia coli strains for extensive protein secretion into the culture medium, to a process for the preparation of the said strains and to the use thereof. <IMAGE>
DK198901865A 1988-04-19 1989-04-18 Process for preparing an E. coli strain for protein secretion of at least 140 mg protein per per liter over 48 hours in the culture medium, prepared by the process E. coli strain and its use DK174508B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3813107 1988-04-19
DE3813107A DE3813107A1 (en) 1988-04-19 1988-04-19 SECRETOR MUTANT OF ESCHERICHIA COLI

Publications (3)

Publication Number Publication Date
DK186589D0 true DK186589D0 (en) 1989-04-18
DK186589A DK186589A (en) 1989-10-20
DK174508B1 DK174508B1 (en) 2003-05-05

Family

ID=6352379

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198901865A DK174508B1 (en) 1988-04-19 1989-04-18 Process for preparing an E. coli strain for protein secretion of at least 140 mg protein per per liter over 48 hours in the culture medium, prepared by the process E. coli strain and its use

Country Status (6)

Country Link
EP (1) EP0338410B1 (en)
JP (1) JP3040402B2 (en)
AT (1) ATE112320T1 (en)
DE (2) DE3813107A1 (en)
DK (1) DK174508B1 (en)
FI (1) FI102544B (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4009268A1 (en) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind SECRETION OF HIRUDIN DERIVATIVES
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
DE4113750A1 (en) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
US5830692A (en) * 1995-03-24 1998-11-03 Consortium Fur Elektrochemische Industrie Gmbh Expression system which can be regulated
DE69735182T2 (en) 1996-08-30 2006-08-03 Aisin Seiki K.K., Kariya Power transmission mechanism
AU4424597A (en) * 1996-09-13 1998-04-02 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
DE102006004871A1 (en) 2006-02-02 2007-08-09 Wacker Chemie Ag Microbial strain useful for producing recombinant proteins comprises a gene coding for a recombinant protein and a gene that codes for a host protein and is mutated to reduce expression of the host protein
ATE501266T1 (en) 2006-09-22 2011-03-15 Wacker Chemie Ag METHOD FOR THE FERMENTATIVE PRODUCTION OF ANTIBODIES
DE102006044841A1 (en) 2006-09-22 2008-04-03 Wacker Chemie Ag Signal peptide for the production of recombinant proteins
DK1903105T3 (en) * 2006-09-22 2010-06-28 Wacker Chemie Ag Process for Fermentative Protein Production
DE102006050332A1 (en) 2006-10-25 2008-04-30 Wacker Chemie Ag DNA construct and method for the fermentative production of fusion proteins
US20100168392A1 (en) * 2007-08-10 2010-07-01 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3279950D1 (en) * 1981-07-30 1989-10-26 Akira Kimura Novel microorganisms derived from microorganisms of the genus escherichia by mutation and their use in the preparation of glutathione
JPS62166881A (en) * 1986-01-16 1987-07-23 Michio Matsuhashi Periplasmic protein-secreting variant strain of escherichia coli
WO1988005821A1 (en) * 1987-01-30 1988-08-11 President And Fellows Of Harvard College Periplasmic protease mutants of escherichia coli

Also Published As

Publication number Publication date
DE58908428D1 (en) 1994-11-03
JP3040402B2 (en) 2000-05-15
EP0338410A2 (en) 1989-10-25
ATE112320T1 (en) 1994-10-15
EP0338410A3 (en) 1991-10-16
DE3813107A1 (en) 1989-11-02
FI891832A0 (en) 1989-04-18
FI102544B1 (en) 1998-12-31
FI891832A (en) 1989-10-20
DK186589A (en) 1989-10-20
FI102544B (en) 1998-12-31
DK174508B1 (en) 2003-05-05
EP0338410B1 (en) 1994-09-28
JPH0235077A (en) 1990-02-05

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Legal Events

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PUP Patent expired