DK1809750T3 - Selection of host cells expressing protein at high levels - Google Patents
Selection of host cells expressing protein at high levelsInfo
- Publication number
- DK1809750T3 DK1809750T3 DK05810935.6T DK05810935T DK1809750T3 DK 1809750 T3 DK1809750 T3 DK 1809750T3 DK 05810935 T DK05810935 T DK 05810935T DK 1809750 T3 DK1809750 T3 DK 1809750T3
- Authority
- DK
- Denmark
- Prior art keywords
- polypeptide
- interest
- host cells
- selectable marker
- cells expressing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a selectable marker polypeptide functional in a eukaryotic host cell, and for ii) a polypeptide of interest, the polypeptide of interest having a translation initiation sequence separate from that of the selectable marker polypeptide, characterized in that the coding sequence for the polypeptide of interest is downstream from the coding sequence for the selectable marker in the multicistronic transcription unit, and the nucleic acid sequence coding for the selectable marker polypeptide comprises a mutation that decreases the translation efficiency of the selectable marker in a eukaryotic host cell. The invention also provides methods for obtaining host cells expressing a polypeptide of interest, the host cells comprising the DNA molecules of the invention. The invention further provides the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules according to the invention.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US62630104P | 2004-11-08 | 2004-11-08 | |
EP04105593 | 2004-11-08 | ||
US69661005P | 2005-07-05 | 2005-07-05 | |
PCT/EP2005/055794 WO2006048459A2 (en) | 2004-11-08 | 2005-11-07 | Selection of host cells expressing protein at high levels |
Publications (1)
Publication Number | Publication Date |
---|---|
DK1809750T3 true DK1809750T3 (en) | 2012-06-25 |
Family
ID=34929819
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK05810935.6T DK1809750T3 (en) | 2004-11-08 | 2005-11-07 | Selection of host cells expressing protein at high levels |
Country Status (8)
Country | Link |
---|---|
US (3) | US20060172382A1 (en) |
CN (1) | CN101090968B (en) |
AT (1) | ATE550432T1 (en) |
DK (1) | DK1809750T3 (en) |
ES (1) | ES2384391T3 (en) |
NZ (1) | NZ553700A (en) |
SI (1) | SI1809750T1 (en) |
ZA (1) | ZA200702046B (en) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK1573058T3 (en) * | 2002-12-18 | 2012-01-16 | Chromagenics Bv | Process for improving protein production |
WO2004056986A2 (en) * | 2002-12-20 | 2004-07-08 | Chromagenics B.V. | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors |
SI1809750T1 (en) | 2004-11-08 | 2012-08-31 | Chromagenics Bv | Selection of host cells expressing protein at high levels |
US20100136616A1 (en) * | 2004-11-08 | 2010-06-03 | Chromagenics B.V. | Selection of Host Cells Expressing Protein at High Levels |
BRPI0517380A (en) * | 2004-11-08 | 2008-10-07 | Chromagenics Bv | DNA molecule, expression cassette, host cell, and methods for generating a host cell expressing a polypeptide of interest and for producing a polypeptide of interest |
US8999667B2 (en) * | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
EP1820856B1 (en) * | 2004-11-18 | 2011-04-27 | National University of Corporation Hiroshima University | Method and kit for expressing protein under regulation of the expression from repeated sequence formed by gene amplification and transformant |
CN101389763B (en) * | 2006-02-21 | 2013-01-23 | 科罗迈吉尼科斯公司 | Selection of host cells expressing protein at high levels |
PT2064337E (en) * | 2006-09-20 | 2012-07-26 | Genzyme Corp | A facs-and reporter protein-based system for high throughput development of therapeutic proteins |
JP5648912B2 (en) * | 2008-02-27 | 2015-01-07 | 国立大学法人北海道大学 | Expression vector for mass production of foreign gene-derived protein using animal cells, and use thereof |
US9284563B2 (en) * | 2009-06-15 | 2016-03-15 | Cellagenics B.V. | Stringent selectable markers |
CN102648285A (en) | 2009-08-06 | 2012-08-22 | Cmc依科斯生技制品公司 | Methods for improving recombinant protein expression |
EP2611915B1 (en) | 2010-09-01 | 2015-04-15 | Cellagenics B.V. | Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression |
US20140243234A1 (en) * | 2011-08-03 | 2014-08-28 | Texas Biomedical Research Institute | Nucleic acid compositions, methods and kits for rapid pairing of affinity agents |
TWI698240B (en) | 2012-05-15 | 2020-07-11 | 澳大利亞商艾佛蘭屈澳洲私營有限公司 | Treatment of amd using aav sflt-1 |
EP3119798B1 (en) | 2014-03-17 | 2020-08-05 | Adverum Biotechnologies, Inc. | Compositions and methods for enhanced gene expression in cone cells |
CN104531699B (en) * | 2014-10-09 | 2017-03-15 | 河南农业大学 | A kind of UCOE controlling element fragments of the pig for strengthening exogenous gene expression |
KR20170137730A (en) | 2015-03-02 | 2017-12-13 | 애드베룸 바이오테크놀로지스, 인코포레이티드 | Composition and method for intravitreal delivery of polynucleotides to retinal cones |
IL258537B2 (en) | 2015-10-09 | 2024-05-01 | Genzyme Corp | Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting |
GB2545763A (en) | 2015-12-23 | 2017-06-28 | Adverum Biotechnologies Inc | Mutant viral capsid libraries and related systems and methods |
WO2018067964A1 (en) | 2016-10-07 | 2018-04-12 | Genzyme Corporation | Early post-transfection isolation of cells (epic) for biologics production |
US20220193266A1 (en) * | 2019-04-30 | 2022-06-23 | Chan Zuckerberg Biohub, Inc. | A novel chromatin-opening element for stable long term gene expression |
CN110484563B (en) * | 2019-07-25 | 2023-04-07 | 新乡医学院 | Mammal cell combined expression vector, expression system, preparation method and application |
EP4247947A1 (en) | 2020-11-19 | 2023-09-27 | Genzyme Corporation | Flow cytometry attenuated reporter expression (flare) multiple reporter system and methods of use thereof |
Family Cites Families (61)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4965196A (en) * | 1983-01-19 | 1990-10-23 | Genentech, Inc. | Polycistronic expression vector construction |
FR2569723B1 (en) * | 1984-08-31 | 1986-09-19 | Centre Nat Rech Scient | APPLICATION OF ANTIBIOTICS FROM THE PHLEOMYCIN FAMILY AS A SELECTION AGENT IN THE FIELD OF GENETIC ENGINEERING |
CA2063799A1 (en) | 1989-07-24 | 1991-01-25 | Frank S. Genbauffe | Prevention of internal initiation |
ATE199023T1 (en) | 1991-03-29 | 2001-02-15 | Genentech Inc | METHODS FOR SELECTING RECOMBINANT HOST CELLS WHICH EXPRESS HIGH AMOUNTS OF A DESIRED PROTEIN |
US6319707B1 (en) * | 1991-08-12 | 2001-11-20 | Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington | Cap-independent multicistronic retroviral vectors |
JP2686025B2 (en) * | 1992-08-25 | 1997-12-08 | 理化学研究所 | Blasticidin S deaminase gene |
DE4228458A1 (en) * | 1992-08-27 | 1994-06-01 | Beiersdorf Ag | Multicistronic expression units and their use |
ES2088838T3 (en) * | 1992-11-13 | 2004-01-01 | Idec Pharmaceuticals Corporation | FULLY ALTERED KOZAK CONSENSUS SEQUENCES INTENDED FOR EXPRESSION IN THE MAMMALS. |
US5648267A (en) * | 1992-11-13 | 1997-07-15 | Idec Pharmaceuticals Corporation | Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same |
AU6498294A (en) | 1993-04-07 | 1994-10-24 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Dna sequence which acts as a chromatin insulator element to protect expressed genes from (cis)-acting regulatory sequences in mammalian cells |
US5610053A (en) * | 1993-04-07 | 1997-03-11 | The United States Of America As Represented By The Department Of Health And Human Services | DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells |
US5658763A (en) * | 1993-10-25 | 1997-08-19 | Creative Biomolecules, Inc. | Methods and compositions for high protein production from non-native DNA |
US5972605A (en) * | 1994-07-07 | 1999-10-26 | Geron Corporation | Assays for regulators of mammalian telomerase expression |
US5561053A (en) * | 1994-08-05 | 1996-10-01 | Genentech, Inc. | Method for selecting high-expressing host cells |
WO1996012008A1 (en) | 1994-10-13 | 1996-04-25 | Merck & Co., Inc. | Synthesis of methylase-resistant genes |
US5627033A (en) | 1995-03-28 | 1997-05-06 | Research & Diagnostics Systems, Inc. | Mammalian expression vectors |
PT1445322E (en) * | 1995-06-15 | 2010-01-19 | Crucell Holland Bv | Packaging systems for human recombinant adenovirus to be used in gene therapy |
US5773695A (en) * | 1996-01-26 | 1998-06-30 | North Carolina State University | Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells |
US6107477A (en) * | 1996-09-26 | 2000-08-22 | Aurora Biosciences Corporation | Non-optimal Kozaks sequences |
US5773779A (en) * | 1997-02-21 | 1998-06-30 | The Lincoln Electric Company | Method and system for welding railroad rails |
WO1998039411A1 (en) | 1997-03-04 | 1998-09-11 | Baxter International Inc. | Adenovirus e1-complementing cell lines |
US5888809A (en) | 1997-05-01 | 1999-03-30 | Icos Corporation | Hamster EF-1α transcriptional regulatory DNA |
US6689606B2 (en) * | 1998-07-21 | 2004-02-10 | M.L. Laboratories Plc | Polynucleotide |
AU771111B2 (en) | 1998-07-21 | 2004-03-11 | Emd Millipore Corporation | A polynucleotide comprising a ubiquitous chromatin opening element (UCOE) |
ATE370247T1 (en) * | 1998-08-14 | 2007-09-15 | Chromagenics Bv | DNA SEQUENCE, METHOD FOR DETECTION AND PRODUCTION THEREOF AND USE THEREOF |
US6800457B2 (en) * | 1998-09-22 | 2004-10-05 | Bristol-Myers Squibb Company | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
US6521419B1 (en) * | 1998-09-22 | 2003-02-18 | Kanakaraju Koduri | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
WO2000023606A1 (en) | 1998-10-22 | 2000-04-27 | Medical College Of Georgia Institute, Inc. | Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors |
AU776752B2 (en) | 1999-06-30 | 2004-09-23 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | DNA binding protein and sequence as insulators having specific enhancer blocking activity for regulation of gene expression |
DE60031419T2 (en) * | 1999-08-25 | 2007-09-27 | Immunex Corp., Thousand Oaks | COMPOSITIONS AND METHODS FOR IMPROVED CELL CULTURE |
CA2389633A1 (en) | 1999-11-01 | 2001-05-10 | Chiron Corporation | Expression vectors, transfection systems, and method of use thereof |
US6558948B1 (en) * | 1999-11-23 | 2003-05-06 | Stefan Kochanek | Permanent amniocytic cell line, its production and use for the production of gene transfer vectors |
AU2001243142A1 (en) | 2000-02-03 | 2001-08-14 | Hyseq, Inc. | Novel nucleic acids and polypeptides |
US6586205B1 (en) * | 2000-02-11 | 2003-07-01 | Millennium Pharmaceuticals, Inc. | 43239 a novel GPCR-like molecule and uses thereof |
US20030166042A1 (en) * | 2000-02-11 | 2003-09-04 | Millennium Pharmaceuticals, Inc. | Novel seven-transmembrane proteins/G-protein coupled receptors |
KR100996016B1 (en) | 2000-09-20 | 2010-11-22 | 밀리포어 코포레이션 | Polynucleotide |
JP4307079B2 (en) | 2001-01-26 | 2009-08-05 | セレキス エスアー | Matrix attachment region and method of use |
US20020155540A1 (en) * | 2001-02-14 | 2002-10-24 | Malla Padidam | Method to reduce transcriptional interference between tandem genes |
WO2002068627A2 (en) * | 2001-02-23 | 2002-09-06 | Novartis Ag | Vector constucts |
FR2821855B1 (en) | 2001-03-09 | 2004-04-02 | Cayla | SYNTHETIC GENES AND BACTERIAL PLASMIDS WITHOUT GIC |
TWI377253B (en) * | 2001-04-16 | 2012-11-21 | Martek Biosciences Corp | Product and process for transformation of thraustochytriales microorganisms |
US20040161817A1 (en) | 2001-06-04 | 2004-08-19 | Corixa Corporation | Compositions and methods for high-level, large-scale production of recombinant proteins |
DK1829971T3 (en) * | 2001-07-04 | 2010-08-16 | Chromagenics Bv | DNA sequences with anti-repressor activity |
EP1273666A1 (en) | 2001-07-04 | 2003-01-08 | Chromagenics B.V. | Method of selecting a DNA sequence with transcription modulating activity using a vector comprising an element with a gene transcription repressing activity |
ITMI20012110A1 (en) * | 2001-10-12 | 2003-04-12 | Keryos Spa | MULTI-CISTRONIC VECTORS USABLE IN GENE TRANSFER PROTOCOLS |
US20040038304A1 (en) | 2002-03-28 | 2004-02-26 | Gala Design, Inc. | Antibody libraries |
AU2003243059A1 (en) * | 2002-06-14 | 2003-12-31 | Chromagenics B.V. | Use of repression blocking sequences in methods for enhancing gene expression |
AU2003238719B2 (en) * | 2002-06-14 | 2008-02-21 | Chromagenics B.V. | A method for simultaneous production of multiple proteins, vectors and cells for use therein |
US7344886B2 (en) * | 2002-11-29 | 2008-03-18 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product |
DK1573058T3 (en) * | 2002-12-18 | 2012-01-16 | Chromagenics Bv | Process for improving protein production |
WO2004056986A2 (en) | 2002-12-20 | 2004-07-08 | Chromagenics B.V. | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors |
US7153659B2 (en) * | 2003-09-05 | 2006-12-26 | Genencor International, Inc. | HPV CD8+ T-cell epitopes |
EA011747B1 (en) * | 2004-07-08 | 2009-06-30 | Хромагеникс Б.В. | A recombinant nucleic acid molecule having anti-repressor activity, and methods for use thereof |
US20100136616A1 (en) * | 2004-11-08 | 2010-06-03 | Chromagenics B.V. | Selection of Host Cells Expressing Protein at High Levels |
US8999667B2 (en) * | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
SI1809750T1 (en) * | 2004-11-08 | 2012-08-31 | Chromagenics Bv | Selection of host cells expressing protein at high levels |
US20060195935A1 (en) * | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
BRPI0517380A (en) * | 2004-11-08 | 2008-10-07 | Chromagenics Bv | DNA molecule, expression cassette, host cell, and methods for generating a host cell expressing a polypeptide of interest and for producing a polypeptide of interest |
US8039230B2 (en) * | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US7968700B2 (en) * | 2006-03-20 | 2011-06-28 | Chromagenics B.V. | Expression augmenting DNA fragments, use thereof, and methods for finding thereof |
JP5501054B2 (en) | 2010-03-25 | 2014-05-21 | 株式会社トクヤマ | Method for producing 3,3-diaminoacrylic acid (1-diphenylmethylazetidin-3-yl) ester acetate |
-
2005
- 2005-11-07 SI SI200531538T patent/SI1809750T1/en unknown
- 2005-11-07 US US11/269,525 patent/US20060172382A1/en not_active Abandoned
- 2005-11-07 ES ES05810935T patent/ES2384391T3/en active Active
- 2005-11-07 AT AT05810935T patent/ATE550432T1/en active
- 2005-11-07 DK DK05810935.6T patent/DK1809750T3/en active
- 2005-11-07 NZ NZ553700A patent/NZ553700A/en not_active IP Right Cessation
- 2005-11-07 CN CN200580037896XA patent/CN101090968B/en not_active Expired - Fee Related
-
2007
- 2007-03-08 ZA ZA200702046A patent/ZA200702046B/en unknown
-
2010
- 2010-08-26 US US12/807,133 patent/US8771984B2/en not_active Expired - Fee Related
-
2014
- 2014-01-27 US US14/165,374 patent/US20140186890A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
SI1809750T1 (en) | 2012-08-31 |
US20060172382A1 (en) | 2006-08-03 |
ZA200702046B (en) | 2008-09-25 |
US20140186890A1 (en) | 2014-07-03 |
US8771984B2 (en) | 2014-07-08 |
US20110014655A1 (en) | 2011-01-20 |
NZ553700A (en) | 2010-09-30 |
CN101090968B (en) | 2011-10-26 |
CN101090968A (en) | 2007-12-19 |
ATE550432T1 (en) | 2012-04-15 |
ES2384391T3 (en) | 2012-07-04 |
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