DK157326B

DK157326B - MEASUREMENT SYSTEM FOR USE BY CONTINUOUS FLUID ANALYSIS

Info

Publication number: DK157326B
Application number: DK578587A
Authority: DK
Inventors: Kim Joergensen; Jan Noerager Rasmussen; Peter Haagensen
Original assignee: Carlsberg Forskningscenter
Priority date: 1987-11-04
Filing date: 1987-11-04
Publication date: 1989-12-11
Also published as: DK578587D0; WO1989004473A1; DK578587A; AU2724888A; DK157326C

Description

DK 157326 BDK 157326 B

Opfindelsen angâr et mâlesystem af den i krav l's indled-ning angivne art. Et sâdant mâlesystem med integrerede blokke til udforelse af forskellige former for kontinuerlig gennemstrBmningsanalyse er vist og beskrevet i dansk patent-5 ansegning nr. 4296/82. Blokkens faste struktur og fysiske stabilitet giver gode betingelser for opnâelse af pâlidelige analyseresultater.The invention relates to a measurement system of the kind specified in the preamble of claim 1. Such a measurement system with integrated blocks for carrying out various forms of continuous flow analysis is shown and described in Danish patent application no. 4296/82. The solid structure and physical stability of the block provide good conditions for obtaining reliable analysis results.

Opfindelsen har til formai at rationalisere brugen af det omhandlede system, og dette formai opnâs ved, at 10 systemet er udformet som angivet i krav l's kendetegnende del, idet udferelse af en bestemt, ensket analyse da blot kræver udvælgelse af den til denne analyse indrettede blok, anbringelse af blokken i detektorhuset og tilslut-ning af nogle fâ væskeledninger.The invention aims to rationalize the use of the present system, and this object is achieved by the system being designed as defined in the characterizing part of claim 1, since the execution of a specific individual analysis requires merely selecting the block adapted for this analysis. , placing the block in the detector housing and connecting some fluid lines.

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Hensigtsmæssige enkeltheder ved mâlesystemet er angivet i krav 2-4, og i krav 5 er angivet en særlig fordelagtig udferelse, hvor blokkens fysiske egenskaber er udnyttet til indbygning af en i og for sig kendt doseringsventil, 20 som er i stand til at dosere meget smâ væskemængder med stor præcision.Suitable details of the measurement system are set forth in claims 2-4, and claim 5 is a particularly advantageous embodiment wherein the physical properties of the block are utilized to incorporate a known dosing valve 20 capable of dosing very small fluid quantities with great precision.

Opfindelsen skal forklares nærmere i det fslgende under henvisning til tegningen, hvor 25 fig. 1 i perspektivisk afbildning viser en analyseblok til brug i mâlesystemet ifelge opfindelsen, fig. 2 viser blokken anbragt i et detektorhus, set fra 30 siden og delvis i snit, fig. 3 samme set fra oven og delvis i snit, og fig. 4 en ændret udferelse af en blok anbragt i detektor-35 huset, set fra siden og delvis i snit.The invention will be explained in more detail below with reference to the drawing, in which Figure 1 is a perspective view of an analysis block for use in the measurement system according to the invention; Figure 2 shows the block disposed in a detector housing, viewed from the side and partly in section; 3 is a top plan view, partly in section, and FIG. 4 is a side elevational view, partly in section, of a modified embodiment of a block disposed in the detector housing.

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DK 157326 BDK 157326 B

Den i fig. 1 viste, i hovedsagen kasseformede blok 10 bestâr af et passende plastmateriale sont f.eks. polyvinyl-chlorid eller vinylacetat, Ved den ene ende har blokken en gennemgâende udboring, der strækker sig fra den ene sidekantflade til den anden, og hvori et i og for sig 5 kendt cylindrisk doseringsventillegeme 11 kan forskydes aksialt som angivet ved pile 12 og 13 mellem to stilling-er, f.eks. ad hydraulisk, magnetisk eller pneumatisk vej. Ventillegemet 11 er udformet med tre rundtgâende riller 14, 15 og 16 med afstande imellem svarende til 10 afstandene mellem de to ventilstillinger, og med rum- fang svarende til den enskede doseringsmængde. I den viste stilling befinder midterrillen 15 sig ud for en indlabskanal 17 for den prsvevæske, der skal tilfores i en nsje doseret mængde, samt ud for en udlabskanal 15 18 for denne væske. De to kanaler 17 og 18 er beliggende pâ diametralt modsatte steder af rillen 15. I denne ventilstilling vil rillen 15 sâledes blive fyldt med væske, der strommer ind gennem kanalen 17 og ud gennem kanalen 18. Forskydes ventillegemet nu mod hejre, altsâ 20 i pilen 12's retning, vil rillen 15 være beliggende mellem en tilferselskanal 19 for en reagensvæske og den ene ende af et kanalsystem 20, der er udformet i blokken 10's underside og dækket af en pâ denne fastlimet plade. Samtidig bliver rillen 14 indskudt i provevæske-25 kredslebet, sa at dette kredslab kun bliver afbrudt i den meget korte tid, det tager for ventilen at skifte stilling.The FIG. 1, generally box-shaped block 10 consists of a suitable plastic material, e.g. polyvinyl chloride or vinyl acetate. At one end, the block has a continuous bore extending from one side edge surface to the other, in which a perpendicular cylindrical metering valve body 11 known per se can be displaced axially as indicated by arrows 12 and 13 between two positions, e.g. by hydraulic, magnetic or pneumatic means. The valve body 11 is formed with three circumferential grooves 14, 15 and 16 with distances between corresponding to 10 the distances between the two valve positions, and with volumes corresponding to the individual dosage amount. In the position shown, the center groove 15 is adjacent to an inlet duct 17 for the pressurized liquid to be supplied in a small dosed amount, and next to an outlet duct 15 18 for this liquid. The two channels 17 and 18 are located in diametrically opposite locations of the groove 15. In this valve position, the groove 15 will thus be filled with liquid flowing through the channel 17 and out through the channel 18. The valve body is now displaced to the right, i.e. 20 in the arrow. 12, the groove 15 will be located between a feed channel 19 for a reagent liquid and one end of a duct system 20 formed in the underside of the block 10 and covered by a fixed adhesive plate. At the same time, the groove 14 is inserted into the test liquid-25 circuit, so that this circuit is interrupted only for the very short time it takes for the valve to change position.

Den anden ende af kanalsystemet 20 er tilsluttet en glas-kuvette 21, som strækker sig gennem en udsparing 22 i 30 den modsat doseringsventilen beliggende ende af blokken 10. Fra kuvetten 21 strommer væsken ud gennem en afgangs-kanal 23.The other end of the duct system 20 is connected to a glass cuvette 21 which extends through a recess 22 in the opposite end of the dosing valve 30 of the block 10. From the cuvette 21, the liquid flows out through a discharge duct 23.

I fig. 2 og 3 er blokken 10 vist anbragt i et kasseformet detektorhus 24, som begrænser et opadtil âbent rum 25 35 med dimensioner, der er afpasset til optagelse af blokken.In FIG. 2 and 3, the block 10 is shown housed in a box-shaped detector housing 24 which limits an upwardly open space 25 35 of dimensions adapted to receive the block.

DK 157326BDK 157326B

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Ved denne ende af huset findes der pâ undersiden en forholdsvis lav udsparing, hvis bund er dækket af et udskifteligt excitationsfilter 27. Fra denne bund udgâr dels en spalte 28, hvorigennem blokkens kuvette 21 kan 5 belyses fra en lyskilde 29 gennem filteret 27, dels et kammer 30, hvori kan være anbragt et ikke vist mâle-organ, f.eks. en fotodiode til mâling af lysstyrken fra lyskilden 29. I det her omhandlede tilfælde tænkes provevæsken at være af en sâdan art, at den bringes 10 til at fluorescere af det af filteret 27 filtrerede lys og sâledes udsende lys med en anden frekvens. Dette lys passerer gennem et udskifteligt emissionsfilter 30 til en ikke vist lysdetektor, der er anbragt i en udsparing 31 i enden af huset 24. Detektoren afgiver 15 et signal, der er et udtryk for koncentrationen af pra- vevæske i reagensvæsken i kuvetten.At this end of the housing there is on the underside a relatively low recess, the bottom of which is covered by an interchangeable excitation filter 27. From this bottom there is partly a slot 28, through which the cuvette 21 of the block 5 can be illuminated from a light source 29 through the filter 27 and partly a chamber 30, in which a measuring means not shown, e.g. a photodiode for measuring the brightness of the light source 29. In the present case, the sample liquid is thought to be such that it causes 10 to fluoresce the light filtered by the filter 27 and emit light at a different frequency. This light passes through an interchangeable emission filter 30 to a light detector not shown located in a recess 31 at the end of the housing 24. The detector 15 emits a signal which reflects the concentration of sample liquid in the reagent liquid in the cuvette.

I fig. 4 er en anden udfarelse af en analyseblok, her betegnet 40, vist anbragt i detektorhuset 24. Denne blok adskiller sig fra blokken 10 ved at hâve kuvetten, som her er betegnet 41, liggende vinkelret pâ blokkens endeflade 20 og ved at hâve en indbygget lyskilde i form af en lysdiode 42 beliggende ved den indre ende af kuvetten °9 adskilt fra denne ved et vindue 43. Et andet vindue 44 afgrænser kuvetten udadtil og adskiller den fra det rum 31 i huset 24, der indeholder filteret 30 og den ikke viste lysdetek-25 tor. I dette tilfælde mâler denne detektor kuvettevæskens dæmpning af lyset fra lysdioden 42 som udtryk for koncentrationen af pravevæske i reagensvæsken.In FIG. 4 is another embodiment of an analysis block, herein designated 40, shown disposed in the detector housing 24. This block differs from the block 10 by raising the cuvette, designated here 41, perpendicular to the end surface 20 of the block and by having a built-in light source in the form of an LED 42 located at the inner end of the cuvette ° 9 separated from it by a window 43. Another window 44 defines the cuvette externally and separates it from the compartment 31 of the housing 24 containing the filter 30 and the light detection not shown. -25 tor. In this case, this detector measures the cuvette fluid attenuation of the light from LED 42 as an expression of the sample liquid concentration in the reagent liquid.

Claims

A measurement system for use in continuous flow analysis of liquids and comprising a plurality of blocks of non-porous, rigid material, in one side surface formed by a pattern of ducts, which is notably covered by a plate and which is connected to a flow cuvette so located at one end of each block that it can be illuminated by a light source and emit or transmit light to an outside detector located on the block, characterized in that the blocks have such common external dimensions and For example, the throughput cuvette has been positioned so that they can each be placed in a common, generally box-shaped detector housing containing a light detector for detecting the light received from the cuvette.

Measurement system according to claim 1, characterized in that the detector housing is formed with a through opening, through which a block cuvette placed in the housing can be illuminated.

Measurement system according to claim 2, characterized in that a replaceable color filter is arranged over the opening.

Measurement system according to any one of the preceding claims, characterized in that an axial known per se is arranged in a bore in each block. movable, cylindrical metering valve body with at least one circumferential groove that can be displaced between a filling position in which it stands in connection with a filling channel formed in the block and an analytical position in which it communicates with one in the block. designed reagent channel, partly with one end of the block's channel pattern.