DK1318198T3 - Fremgangsmåde til fremstilling af et rekombinant polypeptid under tilsætning af en inhibitor for chymotrypsin til cellekulturmediet - Google Patents
Fremgangsmåde til fremstilling af et rekombinant polypeptid under tilsætning af en inhibitor for chymotrypsin til cellekulturmedietInfo
- Publication number
- DK1318198T3 DK1318198T3 DK03000391T DK03000391T DK1318198T3 DK 1318198 T3 DK1318198 T3 DK 1318198T3 DK 03000391 T DK03000391 T DK 03000391T DK 03000391 T DK03000391 T DK 03000391T DK 1318198 T3 DK1318198 T3 DK 1318198T3
- Authority
- DK
- Denmark
- Prior art keywords
- chymotrypsin
- inhibitor
- preparation
- culture medium
- cell culture
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9601855A SE9601855D0 (sv) | 1996-05-14 | 1996-05-14 | Process for producing a protein |
| US1887496P | 1996-05-29 | 1996-05-29 | |
| EP97923382A EP0934424B1 (en) | 1996-05-14 | 1997-05-13 | A process for producing a recombinant polypeptide involving the addition of an inhibitor of metal-dependent proteases or chymotrypsins to the cell culture medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1318198T3 true DK1318198T3 (da) | 2006-07-31 |
Family
ID=27238904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK03000391T DK1318198T3 (da) | 1996-05-14 | 1997-05-13 | Fremgangsmåde til fremstilling af et rekombinant polypeptid under tilsætning af en inhibitor for chymotrypsin til cellekulturmediet |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP1318198B1 (da) |
| DK (1) | DK1318198T3 (da) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990002175A1 (en) * | 1988-08-16 | 1990-03-08 | Novo Nordisk A/S | A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors |
| SE465222C5 (sv) * | 1989-12-15 | 1998-02-10 | Pharmacia & Upjohn Ab | Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning |
-
1997
- 1997-05-13 EP EP03000391A patent/EP1318198B1/en not_active Expired - Lifetime
- 1997-05-13 DK DK03000391T patent/DK1318198T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP1318198A1 (en) | 2003-06-11 |
| EP1318198B1 (en) | 2006-03-22 |
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