DK130082A - PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IINSULINES - Google Patents
PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IINSULINES Download PDFInfo
- Publication number
- DK130082A DK130082A DK130082A DK130082A DK130082A DK 130082 A DK130082 A DK 130082A DK 130082 A DK130082 A DK 130082A DK 130082 A DK130082 A DK 130082A DK 130082 A DK130082 A DK 130082A
- Authority
- DK
- Denmark
- Prior art keywords
- amino acid
- ins
- denotes
- insulin
- ser
- Prior art date
Links
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The B-30 amino acid in insulins is replaced enzymically by reacting an insulin Ins-X, in which X denotes the B-30 amino acid, as the substrate component, with an amine component selected from (a) amino acids of the formula: H-B-OH, (b) where appropriate, N-substituted amino acid amides of the formula: H-B-NR<1>R<2>, in which B denotes an amino acid residue, and R<1> and R<2>, independently of one another, denote hydrogen, amino, hydroxyl, alkyl, cycloalkyl, aryl, heteroaryl or aralkyl, where R<1> and R<2> can furthermore form, together with the nitrogen atom, a heterocyclic group which can contain a further heteroatom, and (c) amino acid esters of the formula H-B-OR<3>, H-B-SR<3> or H-B-SeR<3>, in which B denotes an amino acid residue, and R<3> denotes alkyl, cycloalkyl, aryl, heteroaryl or aralkyl, in the presence of a L-specific serine peptidase or thiolcarboxypeptidase enzyme in an aqueous solution or dispersion at a pH of approx. 7-10.5, in order to form an insulin derivative: Ins-B-OH, Ins-B-NR<1>R<2>, Ins-B-B- NR<1>R<2>, Ins-B-OR<3>, Ins-B-SR<3> or Ins-B-SeR<3>, after which, if desired, a group -NR<1>R<2>, -B- NR<1>R<2>, -OR<3>, -SR<3> or SeR<3> is eliminated, preferably using a carboxypeptidase enzyme. The elimination can also be carried out on derivatives which are obtained by means of other methods. Human insulin is obtained when pig insulin is used as the substrate component and threonin is used as the amino acid which is included in the amine component.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK130082A DK148714C (en) | 1980-07-24 | 1982-03-23 | PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES |
DK320483A DK149616C (en) | 1980-07-24 | 1983-07-12 | PROCEDURE FOR MANUFACTURING INSULINES |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK319780 | 1980-07-24 | ||
DK319780A DK319780A (en) | 1980-07-24 | 1980-07-24 | PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES |
DK8100074 | 1981-07-23 | ||
PCT/DK1981/000074 WO1982000301A1 (en) | 1980-07-24 | 1981-07-23 | Enzymatic replacement of the b-30 amino acid in insulins |
DK130082 | 1982-03-23 | ||
DK130082A DK148714C (en) | 1980-07-24 | 1982-03-23 | PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES |
Publications (3)
Publication Number | Publication Date |
---|---|
DK130082A true DK130082A (en) | 1982-03-23 |
DK148714B DK148714B (en) | 1985-09-09 |
DK148714C DK148714C (en) | 1986-01-27 |
Family
ID=26065552
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK130082A DK148714C (en) | 1980-07-24 | 1982-03-23 | PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES |
DK320483A DK149616C (en) | 1980-07-24 | 1983-07-12 | PROCEDURE FOR MANUFACTURING INSULINES |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK320483A DK149616C (en) | 1980-07-24 | 1983-07-12 | PROCEDURE FOR MANUFACTURING INSULINES |
Country Status (1)
Country | Link |
---|---|
DK (2) | DK148714C (en) |
-
1982
- 1982-03-23 DK DK130082A patent/DK148714C/en not_active IP Right Cessation
-
1983
- 1983-07-12 DK DK320483A patent/DK149616C/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DK148714B (en) | 1985-09-09 |
DK320483D0 (en) | 1983-07-12 |
DK148714C (en) | 1986-01-27 |
DK149616C (en) | 1987-02-02 |
DK149616B (en) | 1986-08-11 |
DK320483A (en) | 1983-07-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PBP | Patent lapsed | ||
PBP | Patent lapsed |