DK130082A - PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IINSULINES - Google Patents

PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IINSULINES Download PDF

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Publication number
DK130082A
DK130082A DK130082A DK130082A DK130082A DK 130082 A DK130082 A DK 130082A DK 130082 A DK130082 A DK 130082A DK 130082 A DK130082 A DK 130082A DK 130082 A DK130082 A DK 130082A
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DK
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Prior art keywords
amino acid
ins
denotes
insulin
ser
Prior art date
Application number
DK130082A
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Danish (da)
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DK148714B (en
DK148714C (en
Inventor
K Breddam
J T Johansen
F Widmer
Original Assignee
Forenede Bryggerier As
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Filing date
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Priority claimed from DK319780A external-priority patent/DK319780A/en
Application filed by Forenede Bryggerier As filed Critical Forenede Bryggerier As
Priority to DK130082A priority Critical patent/DK148714C/en
Publication of DK130082A publication Critical patent/DK130082A/en
Priority to DK320483A priority patent/DK149616C/en
Publication of DK148714B publication Critical patent/DK148714B/en
Application granted granted Critical
Publication of DK148714C publication Critical patent/DK148714C/en

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The B-30 amino acid in insulins is replaced enzymically by reacting an insulin Ins-X, in which X denotes the B-30 amino acid, as the substrate component, with an amine component selected from (a) amino acids of the formula: H-B-OH, (b) where appropriate, N-substituted amino acid amides of the formula: H-B-NR<1>R<2>, in which B denotes an amino acid residue, and R<1> and R<2>, independently of one another, denote hydrogen, amino, hydroxyl, alkyl, cycloalkyl, aryl, heteroaryl or aralkyl, where R<1> and R<2> can furthermore form, together with the nitrogen atom, a heterocyclic group which can contain a further heteroatom, and (c) amino acid esters of the formula H-B-OR<3>, H-B-SR<3> or H-B-SeR<3>, in which B denotes an amino acid residue, and R<3> denotes alkyl, cycloalkyl, aryl, heteroaryl or aralkyl, in the presence of a L-specific serine peptidase or thiolcarboxypeptidase enzyme in an aqueous solution or dispersion at a pH of approx. 7-10.5, in order to form an insulin derivative: Ins-B-OH, Ins-B-NR<1>R<2>, Ins-B-B- NR<1>R<2>, Ins-B-OR<3>, Ins-B-SR<3> or Ins-B-SeR<3>, after which, if desired, a group -NR<1>R<2>, -B- NR<1>R<2>, -OR<3>, -SR<3> or SeR<3> is eliminated, preferably using a carboxypeptidase enzyme. The elimination can also be carried out on derivatives which are obtained by means of other methods. Human insulin is obtained when pig insulin is used as the substrate component and threonin is used as the amino acid which is included in the amine component.
DK130082A 1980-07-24 1982-03-23 PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES DK148714C (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DK130082A DK148714C (en) 1980-07-24 1982-03-23 PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES
DK320483A DK149616C (en) 1980-07-24 1983-07-12 PROCEDURE FOR MANUFACTURING INSULINES

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK319780 1980-07-24
DK319780A DK319780A (en) 1980-07-24 1980-07-24 PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES
DK8100074 1981-07-23
PCT/DK1981/000074 WO1982000301A1 (en) 1980-07-24 1981-07-23 Enzymatic replacement of the b-30 amino acid in insulins
DK130082 1982-03-23
DK130082A DK148714C (en) 1980-07-24 1982-03-23 PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES

Publications (3)

Publication Number Publication Date
DK130082A true DK130082A (en) 1982-03-23
DK148714B DK148714B (en) 1985-09-09
DK148714C DK148714C (en) 1986-01-27

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Family Applications (2)

Application Number Title Priority Date Filing Date
DK130082A DK148714C (en) 1980-07-24 1982-03-23 PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACID IN INSULINES
DK320483A DK149616C (en) 1980-07-24 1983-07-12 PROCEDURE FOR MANUFACTURING INSULINES

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK320483A DK149616C (en) 1980-07-24 1983-07-12 PROCEDURE FOR MANUFACTURING INSULINES

Country Status (1)

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DK (2) DK148714C (en)

Also Published As

Publication number Publication date
DK148714B (en) 1985-09-09
DK320483D0 (en) 1983-07-12
DK148714C (en) 1986-01-27
DK149616C (en) 1987-02-02
DK149616B (en) 1986-08-11
DK320483A (en) 1983-07-12

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