DK1263972T3 - Method of Homologous Recombination - Google Patents

Method of Homologous Recombination

Info

Publication number
DK1263972T3
DK1263972T3 DK01918578.4T DK01918578T DK1263972T3 DK 1263972 T3 DK1263972 T3 DK 1263972T3 DK 01918578 T DK01918578 T DK 01918578T DK 1263972 T3 DK1263972 T3 DK 1263972T3
Authority
DK
Denmark
Prior art keywords
agent
alteration
antibody
homologous recombination
end joining
Prior art date
Application number
DK01918578.4T
Other languages
Danish (da)
Inventor
Evguenii Ivanov
Original Assignee
Shire Human Genetic Therapies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shire Human Genetic Therapies filed Critical Shire Human Genetic Therapies
Application granted granted Critical
Publication of DK1263972T3 publication Critical patent/DK1263972T3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4712—Cystic fibrosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Pulmonology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Led Devices (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)

Abstract

The invention features a method of promoting an alteration at a selected site in a target DNA, e.g., in the chromosomal DNA of a cell. The method includes providing, at the site: (a) a double stranded DNA sequence which includes a selected DNA sequence; (b) an agent which enhances homologous recombination, e.g., a Rad52 protein or a functional fragment thereof; and (c) an agent which inhibits non-homologous end joining, e.g., an agent which inactivates Ku such as an anti-Ku antibody or a Ku-binding oligomer or polymer, and allowing the alteration to occur. The agent which inhibits non-homologous end joining, e.g., a Ku inactivating agent such as an anti-Ku antibody, is preferably provided locally. Components (a), (b), and (c) can be introduced together, which is preferred, or separately.
DK01918578.4T 2000-03-14 2001-03-13 Method of Homologous Recombination DK1263972T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/525,160 US6569681B1 (en) 2000-03-14 2000-03-14 Methods of improving homologous recombination
PCT/US2001/007870 WO2001068882A2 (en) 2000-03-14 2001-03-13 Methods for homologous recombination

Publications (1)

Publication Number Publication Date
DK1263972T3 true DK1263972T3 (en) 2012-01-02

Family

ID=24092175

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01918578.4T DK1263972T3 (en) 2000-03-14 2001-03-13 Method of Homologous Recombination

Country Status (10)

Country Link
US (1) US6569681B1 (en)
EP (1) EP1263972B1 (en)
JP (1) JP2003526376A (en)
AT (1) ATE525472T1 (en)
AU (1) AU2001245637A1 (en)
CA (1) CA2403113A1 (en)
DK (1) DK1263972T3 (en)
MX (1) MXPA02009044A (en)
TR (1) TR200202196T2 (en)
WO (1) WO2001068882A2 (en)

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AU2001279069A1 (en) * 2000-07-27 2002-02-13 University Of Delaware Methods for enhancing targeted gene alteration using oligonucleotides
SG141239A1 (en) * 2001-01-26 2008-04-28 Selexis Sa Matrix attachment regions and methods for use thereof
EP1474522B1 (en) * 2002-01-18 2012-03-07 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
GB0214896D0 (en) * 2002-06-27 2002-08-07 Novartis Forschungsstiftung Gene for increased somatic recombination
FR2846337B1 (en) 2002-10-28 2007-06-22 Centre Nat Rech Scient PROCESS FOR INSERTING A NUCLEIC ACID OF INTEREST INTO A PROCESSARY OR EUKARYOTIC CELL BY HOMOLOGOUS RECOMBINATION
AU2003296438A1 (en) * 2002-12-24 2004-07-29 North Carolina State University Increasing gamete production with a gene switch
JP5396653B2 (en) 2003-10-24 2014-01-22 セレキス エスアー High efficiency gene transfer and expression in mammalian cells by multiple transfection procedures of MAR sequences
JP4050712B2 (en) 2004-02-27 2008-02-20 独立行政法人科学技術振興機構 Method for homologous recombination
DE602005023427D1 (en) 2004-04-02 2010-10-21 Dsm Ip Assets Bv FILAMENTOUS MUSHROOM MUTANES WITH IMPROVED HOMOLOGICAL RECOMBINATION EFFICIENCY
US20060110390A1 (en) * 2004-08-25 2006-05-25 Myogen, Inc. Inhibition of Ku as a treatment for cardiovascular diseases
JP4625318B2 (en) * 2004-12-06 2011-02-02 財団法人野田産業科学研究所 Transformed bacteria with increased homologous recombination frequency
CN101137752B (en) 2005-03-08 2013-04-03 巴斯福植物科学有限公司 Expression enhancing intron sequences
EP2305791A1 (en) 2005-04-15 2011-04-06 North Carolina State University Methods and compositions to modulate adhesion and stress tolerance in bacteria
JP2007222055A (en) * 2006-02-22 2007-09-06 Noda Inst For Scient Res Method for preparing chromosome region deletion strain
JP2008263927A (en) * 2007-04-25 2008-11-06 Noda Inst For Scient Res Method for deletion of large chromosomal region
DK2147107T3 (en) * 2007-05-09 2011-10-24 Novozymes As Method for Expression Cloning Suitable for Selection of Library Clones Producing a Polypeptide of Interest
ES2466916T3 (en) 2007-06-27 2014-06-11 Admune Therapeutics Llc IL-15 and IL-15R alpha complexes and uses thereof
DK2462230T3 (en) * 2009-08-03 2015-10-19 Recombinetics Inc METHODS AND COMPOSITIONS FOR TARGETED RE-MODIFICATION
EP3381937A3 (en) 2009-08-13 2018-10-31 The Johns Hopkins University Methods of modulating immune function
JP5924773B2 (en) * 2009-12-21 2016-05-25 キージーン・エン・フェー Improved technique for transfection of protoplasts
WO2011078662A1 (en) * 2009-12-21 2011-06-30 Keygene N.V. Dsrna for improved genetic modification of plant dna
GB201009732D0 (en) * 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
WO2012168307A2 (en) * 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
US20150307876A1 (en) * 2012-02-10 2015-10-29 Regents Of The University Of Minnesota Dna assimilation
US20150359853A1 (en) 2012-10-24 2015-12-17 Admune Therapeutics Llc Il-15r alpha forms, cells expressing il-15r alpha forms, and therapeutic uses of il-15r alpha and il-15/il-15r alpha complexes
WO2015066205A1 (en) * 2013-10-29 2015-05-07 Rutgers, The State University Of New Jersey Fast genetically modified organism and systems for precise genome editing and targeted genetic manipulation through enhanced homologous recombination
ES2811974T3 (en) 2014-07-29 2021-03-15 Novartis Ag Dose escalation regimens of il-15 and il-15ralpha heterodimer for treating conditions
US10413597B2 (en) 2014-09-29 2019-09-17 Alexion Pharmaceuticals, Inc. Methods of treating Mucopolysaccharidosis IIIB (MPSIIIB)
ES2847252T3 (en) * 2016-02-22 2021-08-02 Caribou Biosciences Inc Procedures for modulating DNA repair results
JP2020505350A (en) 2017-01-20 2020-02-20 ノバルティス アーゲー Combination therapy for the treatment of cancer
WO2018134784A1 (en) 2017-01-20 2018-07-26 Novartis Ag Combination therapy for the treatment of cancer
JP7084021B2 (en) * 2018-01-24 2022-06-14 公立大学法人大阪 Targeting vector
US20210244821A1 (en) 2020-02-05 2021-08-12 Novartis Ag Cho cell expressed het il-15

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US5272071A (en) 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US6010908A (en) 1992-08-21 2000-01-04 The Regents Of The University Of California Gene therapy by small fragment homologous replacement
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
CA2118513A1 (en) * 1992-04-24 1993-11-11 David A. Zarling In vivo homologous sequence targeting in eukaryotic cells
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
PT966683E (en) 1997-01-13 2003-08-29 Kudos Pharm Ltd TESTS AGENTS THERAPY AND DIAGNOSTICS RELATING TO MODULATION OF ACTIVITY OF REPARATION OF CELLULAR DNA
GB9700574D0 (en) 1997-01-13 1997-03-05 Cancer Res Campaign Tech Methods and means relating to retrotransposon and retroviral integration
AU743305C (en) * 1997-01-17 2006-03-30 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US5986055A (en) 1997-11-13 1999-11-16 Curagen Corporation CDK2 interactions
DE19755952C1 (en) 1997-12-16 1998-12-10 Curt Niebling Thin-walled moulded part for electrical and electronic devices
WO1999033971A1 (en) 1997-12-31 1999-07-08 Medical College Of Georgia Research Institute, Inc. Oligomers that bind to ku protein
US6180850B1 (en) 1998-09-02 2001-01-30 Pioneer Hi-Bred International, Inc. Maize Ku70 orthologue and uses thereof
AU771438C (en) 1999-05-05 2004-12-02 Pioneer Hi-Bred International, Inc. Maize Rad50 orthologue and uses thereof

Also Published As

Publication number Publication date
CA2403113A1 (en) 2001-09-20
AU2001245637A1 (en) 2001-09-24
JP2003526376A (en) 2003-09-09
TR200202196T2 (en) 2003-01-21
EP1263972A2 (en) 2002-12-11
ATE525472T1 (en) 2011-10-15
US6569681B1 (en) 2003-05-27
WO2001068882A2 (en) 2001-09-20
EP1263972B1 (en) 2011-09-21
WO2001068882A3 (en) 2002-03-14
MXPA02009044A (en) 2003-04-25

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