DK0646594T3 - Fremgangsmåde til rensning af proteiner - Google Patents

Fremgangsmåde til rensning af proteiner

Info

Publication number
DK0646594T3
DK0646594T3 DK93115953.7T DK93115953T DK0646594T3 DK 0646594 T3 DK0646594 T3 DK 0646594T3 DK 93115953 T DK93115953 T DK 93115953T DK 0646594 T3 DK0646594 T3 DK 0646594T3
Authority
DK
Denmark
Prior art keywords
protein
sorbant
purifying proteins
recovering
solution
Prior art date
Application number
DK93115953.7T
Other languages
Danish (da)
English (en)
Inventor
Lance T Lusk
Henry Goldstein
Original Assignee
Miller Brewing
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miller Brewing filed Critical Miller Brewing
Application granted granted Critical
Publication of DK0646594T3 publication Critical patent/DK0646594T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK93115953.7T 1992-05-14 1993-10-02 Fremgangsmåde til rensning af proteiner DK0646594T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/882,793 US5278284A (en) 1992-05-14 1992-05-14 Protein purification method
EP93115953A EP0646594B1 (en) 1992-05-14 1993-10-02 Protein purification method
JP5251960A JPH07145192A (ja) 1992-05-14 1993-10-07 タンパク質純性化方法及びその純性化確認方法

Publications (1)

Publication Number Publication Date
DK0646594T3 true DK0646594T3 (da) 1997-12-22

Family

ID=27235218

Family Applications (1)

Application Number Title Priority Date Filing Date
DK93115953.7T DK0646594T3 (da) 1992-05-14 1993-10-02 Fremgangsmåde til rensning af proteiner

Country Status (7)

Country Link
US (1) US5278284A (es)
EP (1) EP0646594B1 (es)
JP (1) JPH07145192A (es)
AT (1) ATE154033T1 (es)
DE (1) DE69311337D1 (es)
DK (1) DK0646594T3 (es)
ES (1) ES2105033T3 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
AU4146396A (en) * 1994-11-08 1996-05-31 Regents Of The University Of California, The Temperature-gradient osmometry
CA2167872C (en) * 1995-01-27 2007-04-03 Masaru Nakatani Adsorbent for removing interleukins and tumor necrosis factor, and process for removing the same
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
US6764690B2 (en) * 1996-05-29 2004-07-20 Delsitech Oy Dissolvable oxides for biological applications
US6284250B1 (en) 1998-02-05 2001-09-04 The Henry M. Jackson Foundation For The Advancement Of Military Medicine Simplified method for removing free protein during preparation of protein-polysaccharide conjugates and vaccines using restricted-access media
US6132625A (en) 1998-05-28 2000-10-17 E. I. Du Pont De Nemours And Company Method for treatment of aqueous streams comprising biosolids
US6203711B1 (en) 1999-05-21 2001-03-20 E. I. Du Pont De Nemours And Company Method for treatment of substantially aqueous fluids derived from processing inorganic materials
WO2001094378A1 (en) * 2000-06-08 2001-12-13 The Research Foundation Of State University Of New York Nucleic acid sequences encoding type iii tenebrio antifreeze proteins and method for assaying activity
US6780330B2 (en) 2001-03-09 2004-08-24 E. I. Du Pont De Nemours And Company Removal of biomaterials from aqueous streams
US20030026845A1 (en) * 2001-06-18 2003-02-06 Etzel Lisa R. Process for preparing protein isolate from milk, whey, colostrum, and the like
CA2469815C (en) * 2001-12-21 2011-05-03 Immunex Corporation Methods for purifying protein
US7285269B2 (en) * 2002-12-02 2007-10-23 Amgen Fremont, Inc. Antibodies directed to tumor necrosis factor
US8409647B2 (en) * 2008-08-12 2013-04-02 E. I. Du Pont De Nemours And Company Silica microgels for reducing chill haze
EP2670773A1 (en) 2011-02-01 2013-12-11 Novo Nordisk A/S Purification of insulin
WO2012166705A2 (en) * 2011-05-31 2012-12-06 Brandeis University Supramolecular nanofibers and hydrogels based on amino acid-nucleobase-glycoside conjugates
US10093674B2 (en) 2011-05-31 2018-10-09 Brandeis University Supramolecular nanofibers and hydrogels based on nucleic acids functionalized with nucleobases
GB201411943D0 (en) * 2014-07-03 2014-08-20 Univ Heriot Watt Process and protein product

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2769747A (en) * 1953-02-18 1956-11-06 Armour & Co Process of separation of siliceous impurities from insulin solutions
DE1442334B2 (de) * 1963-08-02 1980-04-24 Wilz-Pauls Geb. Pauls, Rosemarie, Basel (Schweiz) Verfahren zur Behandlung von Bier
GB1215928A (en) * 1967-04-28 1970-12-16 Unilever Ltd A process for reducing haze in beer
US3945988A (en) * 1974-07-16 1976-03-23 Pushpa Khann Process for isolation of insulin from plant source
CH641689A5 (en) * 1976-10-28 1984-03-15 Asahi Chemical Ind Process for preparing a protein adsorbent
JPS5810056A (ja) * 1981-07-10 1983-01-20 株式会社クラレ 血液浄化装置
DE3147842A1 (de) * 1981-12-03 1983-06-23 Hoechst Ag, 6230 Frankfurt "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen"
CA1178222A (en) * 1982-02-04 1984-11-20 Chang W. Chi Composition and method for stabilizing beverages against haze formation
HU190129B (en) * 1983-07-11 1986-08-28 Reanal Finomvegyszergyar,Hu Process for the isolation of carboxypeptidase b enzyme from mammal pancreas
US4528198A (en) * 1983-09-27 1985-07-09 Miller Brewing Company Preparation of low calorie beer with malt extract free of yeast lethal factors
US4636394A (en) * 1984-04-16 1987-01-13 J. E. Siebel Sons' Company, Inc. Method and compositions for chillproofing beverages
US4595578A (en) * 1985-03-22 1986-06-17 Scm Corporation Stabilized silica gel and process for making same
US4684530A (en) * 1985-10-04 1987-08-04 W. R. Grace & Co. Adsorption of proteins from fluids
JPS62207712A (ja) * 1986-03-05 1987-09-12 Fuji Debuison Kagaku Kk ビ−ルの安定化処理用含水シリカゲル
DE3633797C2 (de) * 1986-10-03 1995-08-10 Forssmann Wolf Georg Verfahren zur Gewinnung von biologisch aktiven Eiweissen (Peptiden) aus menschlichem und tierischem Blut (bzw. anderen Körperflüssigkeiten)
GB8707526D0 (en) * 1987-03-30 1987-05-07 Unilever Plc Amorphous silicas
JP2732252B2 (ja) * 1987-12-07 1998-03-25 株式会社ジェイ・エム・エス ヘモグロビン選択吸着剤
US4929560A (en) * 1988-02-03 1990-05-29 Damon Biotech, Inc. Recovery of tissue plasminogen activator
US5086167A (en) * 1988-11-28 1992-02-04 University Of Miami Method of separating proteins

Also Published As

Publication number Publication date
ATE154033T1 (de) 1997-06-15
EP0646594B1 (en) 1997-06-04
US5278284A (en) 1994-01-11
JPH07145192A (ja) 1995-06-06
ES2105033T3 (es) 1997-10-16
EP0646594A1 (en) 1995-04-05
DE69311337D1 (de) 1997-07-10

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