DK0599803T3 - Method for Detecting Antibodies and Antigens - Google Patents

Method for Detecting Antibodies and Antigens

Info

Publication number
DK0599803T3
DK0599803T3 DK93890224T DK93890224T DK0599803T3 DK 0599803 T3 DK0599803 T3 DK 0599803T3 DK 93890224 T DK93890224 T DK 93890224T DK 93890224 T DK93890224 T DK 93890224T DK 0599803 T3 DK0599803 T3 DK 0599803T3
Authority
DK
Denmark
Prior art keywords
carrier
complexes
antigens
natural
detected
Prior art date
Application number
DK93890224T
Other languages
Danish (da)
Inventor
Manfred Dr Schinkinger
Markus Dr Korger
Original Assignee
Sanochemia Pharmazeutica Aktie
Sanochemia Pharmazeutika Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AT0232192A external-priority patent/AT399781B/en
Priority claimed from AT0006593A external-priority patent/AT402674B/en
Application filed by Sanochemia Pharmazeutica Aktie, Sanochemia Pharmazeutika Ag filed Critical Sanochemia Pharmazeutica Aktie
Application granted granted Critical
Publication of DK0599803T3 publication Critical patent/DK0599803T3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

To detect an antibody contained in a sample, the sample is introduced into a reaction mixture which contains a particulate carrier on which an antigen which is specific for the antibody to be detected, such as, for example, natural or recombinant viral antigens, natural or recombinant bacterial antigens, natural or recombinant fungal antigens or other natural or recombinant cell-specific antigens are immobilised, and a conjugate of at least one protein reactive with the Fc fragment of antibodies, and of an indicator bound to this protein. Incubation is then carried out in order to form complexes of conjugate and antibodies and, at the same time, to bind these complexes to the carrier. After this, the carrier and aggregates of carrier and complexes containing at least one antibody to be detected are separated from the liquid reaction mixture and from complexes which contain only antibodies which are not to be detected, and, without additional immunochemical reaction, a test is carried out for the presence of complexes of conjugate and the antibody to be detected which are bound to the carrier. <IMAGE>
DK93890224T 1992-11-23 1993-11-15 Method for Detecting Antibodies and Antigens DK0599803T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0232192A AT399781B (en) 1992-11-23 1992-11-23 Antibody detedction by reaction with specific antigen on carrier particle - in presence of labelled protein reacted with Fc fragment, forming easily sepd. and detected aggregate, also similar methods for antigens
AT0006593A AT402674B (en) 1993-01-18 1993-01-18 Antibody detedction by reaction with specific antigen on carrier particle - in presence of labelled protein reacted with Fc fragment, forming easily sepd. and detected aggregate, also similar methods for antigens

Publications (1)

Publication Number Publication Date
DK0599803T3 true DK0599803T3 (en) 2000-07-03

Family

ID=25591311

Family Applications (1)

Application Number Title Priority Date Filing Date
DK93890224T DK0599803T3 (en) 1992-11-23 1993-11-15 Method for Detecting Antibodies and Antigens

Country Status (8)

Country Link
EP (1) EP0599803B1 (en)
JP (1) JPH06281652A (en)
AT (1) ATE191565T1 (en)
DE (1) DE59309999D1 (en)
DK (1) DK0599803T3 (en)
ES (1) ES2056041T3 (en)
GR (2) GR940300070T1 (en)
PT (1) PT599803E (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
US20060127960A1 (en) * 2002-11-26 2006-06-15 Defense Research & Development Organisation Process for preparation of an agglutination reagent for rapid detection of typhoid
DE102005001362A1 (en) * 2005-01-11 2006-07-20 Protagen Ag Method and device for quantifying proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2039025T3 (en) * 1987-11-16 1993-08-16 Janssen Pharmaceutica N.V. AN ADDED TO DETERMINE COMBINABLE SUBSTANCES.
AU613350B2 (en) * 1988-01-12 1991-08-01 Genelabs Technologies, Inc. Htlv-i peptide antigen and assay
GB8800702D0 (en) * 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor

Also Published As

Publication number Publication date
EP0599803B1 (en) 2000-04-05
PT599803E (en) 2000-07-31
GR940300070T1 (en) 1994-10-31
GR3033389T3 (en) 2000-09-29
EP0599803A1 (en) 1994-06-01
ES2056041T1 (en) 1994-10-01
JPH06281652A (en) 1994-10-07
DE59309999D1 (en) 2000-05-11
ES2056041T3 (en) 2000-08-01
ATE191565T1 (en) 2000-04-15

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