DK0456698T3 - Hidtil ukendte ekspressionsvektorer og fremgangsmåder til intracellulær proteinekspression - Google Patents

Hidtil ukendte ekspressionsvektorer og fremgangsmåder til intracellulær proteinekspression

Info

Publication number
DK0456698T3
DK0456698T3 DK90902632T DK90902632T DK0456698T3 DK 0456698 T3 DK0456698 T3 DK 0456698T3 DK 90902632 T DK90902632 T DK 90902632T DK 90902632 T DK90902632 T DK 90902632T DK 0456698 T3 DK0456698 T3 DK 0456698T3
Authority
DK
Denmark
Prior art keywords
methods
recombinant dna
dna molecules
intracellular protein
novel
Prior art date
Application number
DK90902632T
Other languages
Danish (da)
English (en)
Inventor
Ilkka Palva
Original Assignee
Spectrum Medical Sciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spectrum Medical Sciences Ltd filed Critical Spectrum Medical Sciences Ltd
Application granted granted Critical
Publication of DK0456698T3 publication Critical patent/DK0456698T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK90902632T 1989-02-10 1990-02-08 Hidtil ukendte ekspressionsvektorer og fremgangsmåder til intracellulær proteinekspression DK0456698T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30886189A 1989-02-10 1989-02-10
PCT/FI1990/000041 WO1990009448A1 (en) 1989-02-10 1990-02-08 Novel expression vectors and methods for intracellular protein production

Publications (1)

Publication Number Publication Date
DK0456698T3 true DK0456698T3 (da) 1999-04-12

Family

ID=23195696

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90902632T DK0456698T3 (da) 1989-02-10 1990-02-08 Hidtil ukendte ekspressionsvektorer og fremgangsmåder til intracellulær proteinekspression

Country Status (7)

Country Link
EP (1) EP0456698B1 (es)
AT (1) ATE167897T1 (es)
AU (1) AU649606B2 (es)
DE (1) DE69032453T2 (es)
DK (1) DK0456698T3 (es)
ES (1) ES2120409T3 (es)
WO (1) WO1990009448A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153398A (en) * 1997-12-24 2000-11-28 The University Of Chicago Method to identify specific inhibitors of IMP dehydrogenase
WO2023117970A1 (en) 2021-12-20 2023-06-29 Basf Se Method for improved production of intracellular proteins in bacillus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1340373C (en) * 1986-01-28 1999-02-02 Rino Rappuoli Cloning and sequencing of the dna fragment which codes for the five subunits of the pertussis toxin, a hybrid plasmid containing the dna fragment and micro-organisms transformed by the hybrid plasmid and capable of expressing all or some of the subunits of the pertussis toxin
IT1202612B (it) * 1987-03-03 1989-02-09 Eniricerche Spa Plasmidi ricombinanti utili per l'espressione in bacillus di proteine eterologhe

Also Published As

Publication number Publication date
AU649606B2 (en) 1994-06-02
EP0456698A1 (en) 1991-11-21
ES2120409T3 (es) 1998-11-01
WO1990009448A1 (en) 1990-08-23
AU5034490A (en) 1990-09-05
DE69032453T2 (de) 1999-03-18
ATE167897T1 (de) 1998-07-15
EP0456698B1 (en) 1998-07-01
DE69032453D1 (de) 1998-08-06

Similar Documents

Publication Publication Date Title
FR19C1020I1 (fr) Nouvelles molecules b7-4 et leurs utilisations
DK1218504T3 (da) GL50 molekyler og anvendelser deraf
EA199700244A1 (ru) Слитый белок, экспрессирующий вектор, рекомбинантный штамм, вакцинный препарат
IE883612L (en) Vectors and compounds for direct expression of activated human protein c
EP1009752A4 (en) NOVEL TANGO-77 PROTEIN FAMILY MOLECULES AND USES THEREOF
DE69837085D1 (de) Crsp-proteine (cystein-reiche, sekretierte proteine), die dafür kodierenden nukleinsäuren und ihre verwendungen
ATE419358T1 (de) Gebrauchsverfaheren eines neuen lysyloxidase- verwandten proteins
TR27970A (tr) Bitki kofulu icine hedeflemede kullanilan yeni sinyal dizimleri.
DE69837086D1 (de) Verbesserte prokariontische proteinenexpression
DK0456698T3 (da) Hidtil ukendte ekspressionsvektorer og fremgangsmåder til intracellulær proteinekspression
DK0429600T3 (da) SAF-polypeptid, saf-gen, saf-promotor og anvendelse til ekspression i Streptomyces
EP1412374A4 (en) NEW PGC-1 ISOFORMS AND THEIR USES
DE69634757D1 (de) Vektor zur expression von n-terminal verlängerten proteinen in hefezellen
DK0805204T3 (da) Bitestikel - specifikt receptorprotein og anvendelse heraf
DE60142356D1 (de) 18480 humane proteinkinasemoleküle und ihre verwendungen
DK27097A (da) Minactivin og fremgangsmåde til fremstilling deraf
WO2001073074A3 (en) 18806, a novel trypsin serine protease-like molecule and uses thereof
Palva Expression vectors and methods for intracellular protein production in bascillus
WO2001064919A3 (en) 18036, a novel calpain-like protease and uses thereof
AU2002234132A1 (en) 13245, a novel human myotonic dystrophy type protein kinase and uses therefor
ATE476194T1 (de) Ein mitglied der menschlichen proteinkinasefamilie, und verwendungen dafür
DK1297151T3 (da) Nye 14911-proteinkinasemolekyler og anvendelser deraf
WO2003042371A3 (en) 55053, a novel human eukaryotic kinase and uses therefor
ECSP993285A (es) Sintesis recombinante de beta lipotropina y otros peptidos