DK0435226T3 - Phagocytosis test - Google Patents

Phagocytosis test

Info

Publication number
DK0435226T3
DK0435226T3 DK90125282.5T DK90125282T DK0435226T3 DK 0435226 T3 DK0435226 T3 DK 0435226T3 DK 90125282 T DK90125282 T DK 90125282T DK 0435226 T3 DK0435226 T3 DK 0435226T3
Authority
DK
Denmark
Prior art keywords
phagocytosis
added
fluorescence
phagocytosis test
leukocytes
Prior art date
Application number
DK90125282.5T
Other languages
Danish (da)
Inventor
Thomas C Dr Med Nebe
Original Assignee
Orpegen Med Molekularbioforsch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orpegen Med Molekularbioforsch filed Critical Orpegen Med Molekularbioforsch
Application granted granted Critical
Publication of DK0435226T3 publication Critical patent/DK0435226T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/10—DNA staining
    • C12Q2304/13—Propidium

Abstract

To determine the phagocytosis activity of leukocytes, a sample of whole blood is mixed with a defined amount of bacteria, preferably E. coli, labelled with FITC, and incubated with shaking at a temperature of 30 to 45 DEG C for 5 to 60 minutes, the phagocytosis is stopped by cooling, a quenching substance is added, the erythrocytes present in the solution are lysed, a fluorescent DNA dye is added, and subsequently the fluorescence is quantified in a fluorescence cytometer.
DK90125282.5T 1989-12-29 1990-12-21 Phagocytosis test DK0435226T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3943325 1989-12-29
DE4014393A DE4014393A1 (en) 1989-12-29 1990-05-04 PHAGOCYTOSE TEST

Publications (1)

Publication Number Publication Date
DK0435226T3 true DK0435226T3 (en) 1995-10-16

Family

ID=25888629

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90125282.5T DK0435226T3 (en) 1989-12-29 1990-12-21 Phagocytosis test

Country Status (6)

Country Link
EP (1) EP0435226B1 (en)
AT (1) ATE123071T1 (en)
DE (2) DE4014393A1 (en)
DK (1) DK0435226T3 (en)
ES (1) ES2023631T3 (en)
GR (1) GR910300103T1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4117459C2 (en) * 1991-05-28 1994-09-22 Orpegen Med Molekularbioforsch BURST test
DE4334935C1 (en) * 1993-10-13 1995-03-09 Luciana Husfeld Method for determining phagocytosis and / or killing ability
FR2753536B1 (en) * 1996-09-16 1998-11-20 Aetsrn METHOD FOR EVALUATION OF PHAGOCYTOSIS OF TARGET CELLS BY MACROPHAGES IN VITRO; DIAGNOSTIC APPLICATIONS AND APPLICATION TO QUALITY CONTROL OF CELL STOCKS
DE19950262C2 (en) * 1999-10-18 2001-09-13 Martin Foermer Procedure for performing a flow cytometric phagocytosis test
JPWO2002101037A1 (en) * 2001-05-31 2005-04-07 扶桑薬品工業株式会社 Method for evaluating phagocytic function and use thereof
DE10361243B4 (en) * 2003-12-22 2007-07-12 Universität Ulm Investigation procedure for phagocytosis
CN108300754A (en) * 2018-01-03 2018-07-20 王洵 It is a kind of to detect cytophagic Fluorescence kit and preparation method thereof

Also Published As

Publication number Publication date
DE4014393A1 (en) 1991-07-04
DE59009136D1 (en) 1995-06-29
EP0435226A1 (en) 1991-07-03
ES2023631T3 (en) 1995-08-16
GR910300103T1 (en) 1991-12-10
ES2023631A4 (en) 1992-02-01
ATE123071T1 (en) 1995-06-15
EP0435226B1 (en) 1995-05-24

Similar Documents

Publication Publication Date Title
Baskurt et al. Erythrocyte aggregation tendency and cellular properties in horse, human, and rat: a comparative study
DE69223931T2 (en) Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood
Sorger et al. A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers
BRPI0200585B8 (en) process for identifying and counting biological cells in a blood sample.
US6977156B2 (en) Flow cytometry reagent and system
IL103054A0 (en) Methods and reagent compositions for characterizing and identifying reticulocytes in whole blood
FI892926A0 (en) Process for analyzing cellular components of a liquid
US6271035B1 (en) Methods and compositions for rapid staining of nucleic acids in whole cells
DK0435226T3 (en) Phagocytosis test
Barry et al. Relationship of colominic acid (poly N-acetylneuraminic acid) to bacteria which contain neuraminic acid
BR9814415A (en) "method for developing, testing and using macromolecule associates and complex aggregates for increased payload and controllable decoupling rates"
Jackson et al. Cytofluorograf detection of Plasmodium yoelii, Trypanosoma gambiense, and Trypanosoma equiperdum by laser excited fluorescence of stained rodent blood
Hobson et al. Serological tests of a relationship between rumen selenomonads in vitro and in vivo
PT83515A (en) PROCESS FOR THE REALIZATION OF SUBPOPULATION ANALYSIS AND PREPARATION OF REAGENTS FOR THE SAME
JPS59108959A (en) Method of determining histamine
US5902733A (en) Test system for determining the activity of natural killer cells
KR890700640A (en) Latex for coloring latex and reagents
Simpson et al. Rhodanile blue; a rapid and selective stain for Heinz bodies
EP0231127A3 (en) Composition, element and method using polymeric mordants to increase the intensity of rigid fluorescent dyes
Clark Red cell lactic acid dehydrogenase in the family Macropodidae
Boulanger et al. A circulating protein that depolarizes cells increases after hemorrhage in dogs
Turner et al. Biochemical characterization of the genetic variants of human phosphoglycolate phosphatase (PGP)
GEU1998362Y (en) Method for Determining of Leukocytes in the Biological Material
Van Der Lely et al. Detection of incorporated iododeoxyuridine in colonies by immunoperoxidase staining: A novel method to measure the proportion of cycling colony-forming cells
Mayall et al. Quantitative cell-adhesion assay for Clostridium difficile cytotoxin