DK0435226T3 - Phagocytosis test - Google Patents

Phagocytosis test

Info

Publication number
DK0435226T3
DK0435226T3 DK90125282.5T DK90125282T DK0435226T3 DK 0435226 T3 DK0435226 T3 DK 0435226T3 DK 90125282 T DK90125282 T DK 90125282T DK 0435226 T3 DK0435226 T3 DK 0435226T3
Authority
DK
Denmark
Prior art keywords
phagocytosis
added
fluorescence
phagocytosis test
leukocytes
Prior art date
Application number
DK90125282.5T
Other languages
Danish (da)
Inventor
Thomas C Dr Med Nebe
Original Assignee
Orpegen Med Molekularbioforsch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orpegen Med Molekularbioforsch filed Critical Orpegen Med Molekularbioforsch
Application granted granted Critical
Publication of DK0435226T3 publication Critical patent/DK0435226T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/10—DNA staining
    • C12Q2304/13—Propidium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

To determine the phagocytosis activity of leukocytes, a sample of whole blood is mixed with a defined amount of bacteria, preferably E. coli, labelled with FITC, and incubated with shaking at a temperature of 30 to 45 DEG C for 5 to 60 minutes, the phagocytosis is stopped by cooling, a quenching substance is added, the erythrocytes present in the solution are lysed, a fluorescent DNA dye is added, and subsequently the fluorescence is quantified in a fluorescence cytometer.
DK90125282.5T 1989-12-29 1990-12-21 Phagocytosis test DK0435226T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3943325 1989-12-29
DE4014393A DE4014393A1 (en) 1989-12-29 1990-05-04 PHAGOCYTOSE TEST

Publications (1)

Publication Number Publication Date
DK0435226T3 true DK0435226T3 (en) 1995-10-16

Family

ID=25888629

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90125282.5T DK0435226T3 (en) 1989-12-29 1990-12-21 Phagocytosis test

Country Status (6)

Country Link
EP (1) EP0435226B1 (en)
AT (1) ATE123071T1 (en)
DE (2) DE4014393A1 (en)
DK (1) DK0435226T3 (en)
ES (1) ES2023631T3 (en)
GR (1) GR910300103T1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4117459C2 (en) * 1991-05-28 1994-09-22 Orpegen Med Molekularbioforsch BURST test
DE4334935C1 (en) * 1993-10-13 1995-03-09 Luciana Husfeld Method for determining phagocytosis and / or killing ability
FR2753536B1 (en) * 1996-09-16 1998-11-20 Aetsrn METHOD FOR EVALUATION OF PHAGOCYTOSIS OF TARGET CELLS BY MACROPHAGES IN VITRO; DIAGNOSTIC APPLICATIONS AND APPLICATION TO QUALITY CONTROL OF CELL STOCKS
DE19950262C2 (en) * 1999-10-18 2001-09-13 Martin Foermer Procedure for performing a flow cytometric phagocytosis test
JPWO2002101037A1 (en) * 2001-05-31 2005-04-07 扶桑薬品工業株式会社 Method for evaluating phagocytic function and use thereof
DE10361243B4 (en) * 2003-12-22 2007-07-12 Universität Ulm Investigation procedure for phagocytosis
CN108300754A (en) * 2018-01-03 2018-07-20 王洵 It is a kind of to detect cytophagic Fluorescence kit and preparation method thereof

Also Published As

Publication number Publication date
EP0435226B1 (en) 1995-05-24
ES2023631T3 (en) 1995-08-16
ATE123071T1 (en) 1995-06-15
DE4014393A1 (en) 1991-07-04
EP0435226A1 (en) 1991-07-03
GR910300103T1 (en) 1991-12-10
ES2023631A4 (en) 1992-02-01
DE59009136D1 (en) 1995-06-29

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