DK0428881T3 - RNA with endonuclease and antisense activity, its preparation and use - Google Patents

RNA with endonuclease and antisense activity, its preparation and use

Info

Publication number
DK0428881T3
DK0428881T3 DK90120271.3T DK90120271T DK0428881T3 DK 0428881 T3 DK0428881 T3 DK 0428881T3 DK 90120271 T DK90120271 T DK 90120271T DK 0428881 T3 DK0428881 T3 DK 0428881T3
Authority
DK
Denmark
Prior art keywords
rna
endonuclease
preparation
antisense activity
ribozyme
Prior art date
Application number
DK90120271.3T
Other languages
Danish (da)
Inventor
Eugen Dr Uhlmann
Hubert Dr Muellner
Peter Dr Eckes
Rudolf Dr Schneider
Bernardus Dr Uijtewaal
Original Assignee
Hoechst Schering Agrevo Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Schering Agrevo Gmbh filed Critical Hoechst Schering Agrevo Gmbh
Application granted granted Critical
Publication of DK0428881T3 publication Critical patent/DK0428881T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Communicable Diseases (AREA)
  • Veterinary Medicine (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Host cells can be transformed so that they express ribozyme RNA and antisense RNA, which are linked together in the loop of the ribozyme. The RNA molecules can, for example, be complementary to a particular viral RNA. Plants transformed with genes coding for such RNA display significantly improved defences against viruses.
DK90120271.3T 1989-10-25 1990-10-23 RNA with endonuclease and antisense activity, its preparation and use DK0428881T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3935473A DE3935473A1 (en) 1989-10-25 1989-10-25 RNA WITH ENDONUCLEASE AND ANTISENSE ACTIVITY, THEIR PRODUCTION AND THEIR USE

Publications (1)

Publication Number Publication Date
DK0428881T3 true DK0428881T3 (en) 1995-10-30

Family

ID=6392144

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90120271.3T DK0428881T3 (en) 1989-10-25 1990-10-23 RNA with endonuclease and antisense activity, its preparation and use

Country Status (10)

Country Link
EP (1) EP0428881B1 (en)
JP (1) JPH044877A (en)
KR (1) KR910008136A (en)
AT (1) ATE124450T1 (en)
DE (2) DE3935473A1 (en)
DK (1) DK0428881T3 (en)
ES (1) ES2076275T3 (en)
HU (1) HUT58794A (en)
IE (1) IE67653B1 (en)
NZ (1) NZ235789A (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001786A1 (en) * 1990-07-26 1992-02-06 Foundation For Research And Technology - Hellas (Fo.R.T.H.) Institute Of Molecular Biology & Biotechnology Portable ribozyme cassettes, dna sequences containing them, ribozymes encoded by these dna sequences, and compositions containing these ribozymes
JP3110460B2 (en) * 1992-04-17 2000-11-20 麒麟麦酒株式会社 Plants resistant to multiple viruses and methods for producing the same
PT652705E (en) * 1992-06-29 2006-05-31 Gene Shears Pty Ltd NUCLEIC ACIDS AND METHODS FOR THEIR USE IN COMBATING VIRAL PATHOGENS
US20040005546A1 (en) 2002-02-28 2004-01-08 Oncolytics Biotech Inc. Use of ribozymes in the detection of adventitious agents
AR047598A1 (en) * 2004-02-10 2006-01-25 Monsanto Technology Llc TRANSGENIZED CORN SEED WITH GREATER AMINO ACID CONTENT
US7855323B2 (en) 2004-02-10 2010-12-21 Monsanto Technology Llc Recombinant DNA for gene suppression
CN101128588A (en) 2004-08-11 2008-02-20 孟山都技术有限公司 Enhanced zein reduction in transgenic corn seed

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ227332A (en) * 1987-12-15 1991-08-27 Gene Shears Pty Ltd Ribozymes, production and use

Also Published As

Publication number Publication date
ATE124450T1 (en) 1995-07-15
EP0428881A1 (en) 1991-05-29
IE903825A1 (en) 1991-05-08
JPH044877A (en) 1992-01-09
AU6499590A (en) 1991-05-02
HU906538D0 (en) 1991-04-29
DE59009334D1 (en) 1995-08-03
IE67653B1 (en) 1996-04-17
AU627944B2 (en) 1992-09-03
NZ235789A (en) 1992-07-28
DE3935473A1 (en) 1991-05-02
ES2076275T3 (en) 1995-11-01
EP0428881B1 (en) 1995-06-28
HUT58794A (en) 1992-03-30
KR910008136A (en) 1991-05-30

Similar Documents

Publication Publication Date Title
DK0421376T3 (en) Multifunctional RNA with self-processing activity, its preparation and use
DE69836092D1 (en) RECOMBINATORY CLONING USING NUCLEAR FACILITIES WITH RECOMBINATION CENTERS
DE3681787D1 (en) EXPRESSION OF FOREIGN GENETIC MATERIAL IN EPITHELIC CELLS.
DE602004029608D1 (en) MUTANT HERPES-SIMPLEX VIRUS AND USE THEREOF IN THE TREATMENT OF SQUAMOUS PEPPER CARCINOMA
NZ243500A (en) Nucleic acid encoding dihydrokaempferol hydroxylating enzyme (dhk) and its use in producing transgenic flowering plants
DK159100C (en) PROCEDURE FOR PREPARING A LIQUID VIRAL VACCINE CONTAINING A STABILIZER AND STABILIZER THAT CAN BE USED IN SUCH A PROCEDURE
DE69331946D1 (en) nucleozymes
ES8105388A1 (en) DNA transfer vector, host transformed with it, vaccine, and their production.
HK1019233A1 (en) Animal cells and processes for the replication of influenza viruses
DK0428881T3 (en) RNA with endonuclease and antisense activity, its preparation and use
DK589085A (en) RECOMBINATE DNA EXPRESSION VECTOR, FUNCTIONAL AND SELECTIVE IN PLANTS
EP0019218A3 (en) Influenza virus vaccines, process for preparing them, process for propagating influenza viruses and cell culture inoculated with influenza virus
DK0409628T3 (en) Compositions and Methods for Modulating Levels of Endogenous Cytokines
FI890116A (en) ICKE-REVERTERANDE RNA VIRUS.
BR8602126A (en) PROCESS FOR THE INTRODUCTION OF VIRAL DNA IN VEGETABLE MATERIAL, BACTERIA, VEGETABLE MATERIAL, AND PLANTS AND USE OF MICROORGANISMS
EP0592685A4 (en) Plant resistant to two or more viruses and preparation thereof.
Bigg Photography and labour history of astrometry: the Carte du Ciel
NO854977L (en) TOEY softener in concentrated and stable, non-aqueous form.
ES2067514T3 (en) DNA SEQUENCE OF SOLANUM TUBEROSUM STIMULATED BY VULNERATION, AND ITS USE.
Price et al. Serum-free medium without animal components for virus production
Bernhardt et al. Induced growth striations in Quartz Crystals
AU3964189A (en) Complete nucleotidic sequence of the complementary dna of the genomic rna of the potyvirus, genes coding for the potyvirus capside protein and application of said genes to the creation of potyvirus-resistant transgenic plants
ATA71388A (en) DNA AND RNA MOLECULES OF THE WESTERN SUBTYPE OF THE FSME VIRUS, POLYPEPTIDES CODED BY THESE MOLECULES, AND THEIR USE
ATE132527T1 (en) NON-REMUTATING RNA VIRUSES
姚慕生 IDEALS OF TENSOR PRODUCTS OF DIVISION ALGEBRAS AND PRIMITIVE ALGEBRAS