DK0379216T3 - Method and reagent for the determination of immunologically detectable substances - Google Patents

Method and reagent for the determination of immunologically detectable substances

Info

Publication number
DK0379216T3
DK0379216T3 DK90101095.9T DK90101095T DK0379216T3 DK 0379216 T3 DK0379216 T3 DK 0379216T3 DK 90101095 T DK90101095 T DK 90101095T DK 0379216 T3 DK0379216 T3 DK 0379216T3
Authority
DK
Denmark
Prior art keywords
determination
solid phase
bound
binding
detectable substances
Prior art date
Application number
DK90101095.9T
Other languages
Danish (da)
Inventor
Christian Dr Rer Nat Klein
Peter Dr Rer Nat Kirch
Hubert Dr Rer Nat Bayer
Erhard Dr Rer Nat Kopetzki
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE3901638A external-priority patent/DE3901638C2/en
Priority claimed from DE19893924239 external-priority patent/DE3924239A1/en
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Application granted granted Critical
Publication of DK0379216T3 publication Critical patent/DK0379216T3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

For the determination of immunologically detectable substances by the principle of the heterogeneous immunoassay by incubation with at least two receptors R1 and R2, of which R1 mediates the binding to the solid phase and R2 is a conjugate of a ligand which is able to bind to a substance to be determined and of a label, using a solid phase to which a reaction component capable of specific binding is bound, separation of the solids from the liquid phase and determination of the label in one of the two phases, the solid phase is a material to whose surface are bound partners capable of specific binding, and the sample solution is incubated with a plurality of different receptors R1, where each receptor R1 has both binding sites for the partner which is capable of specific binding and is bound to the solid phase and binding sites for one of the substances to be determined. <IMAGE>
DK90101095.9T 1989-01-20 1990-01-19 Method and reagent for the determination of immunologically detectable substances DK0379216T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3901638A DE3901638C2 (en) 1988-05-25 1989-01-20 Method for determining an immunologically detectable substance and suitable reaction vessel
DE19893924239 DE3924239A1 (en) 1989-07-21 1989-07-21 Simultaneous heterogeneous immunoassay of several analyte

Publications (1)

Publication Number Publication Date
DK0379216T3 true DK0379216T3 (en) 1994-10-31

Family

ID=25876968

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90101095.9T DK0379216T3 (en) 1989-01-20 1990-01-19 Method and reagent for the determination of immunologically detectable substances

Country Status (8)

Country Link
EP (1) EP0379216B1 (en)
JP (1) JPH0827284B2 (en)
AT (1) ATE107030T1 (en)
AU (1) AU633041B2 (en)
CA (1) CA2008100A1 (en)
DE (1) DE59005973D1 (en)
DK (1) DK0379216T3 (en)
ES (1) ES2055172T3 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4211108A1 (en) * 1992-04-03 1993-10-07 Boehringer Mannheim Gmbh Virus solution for use in immunoassays
DE4218257A1 (en) * 1992-06-03 1993-12-09 Behringwerke Ag Method for immunochemical determination of an analyte
WO1994003807A1 (en) * 1992-08-03 1994-02-17 Gec-Marconi Limited Separation method
GB2271634B (en) * 1992-09-18 1997-04-09 Marconi Gec Ltd Separation method
DE4236189A1 (en) * 1992-10-27 1994-04-28 Boehringer Mannheim Gmbh Method for the simultaneous determination of antigens and antibodies
GB2273157A (en) * 1992-11-27 1994-06-08 Marconi Gec Ltd Immunological detection/separation using a plurality of immobilised binding agents
BR9404967A (en) * 1993-04-14 1999-06-15 Int Murex Tech Corp Immunoassay
GB2282884A (en) * 1993-04-14 1995-04-19 Int Murex Tech Corp Immunoassay
US6383740B2 (en) 1999-07-30 2002-05-07 Bioergonomics, Inc. Methods for simultaneously detecting both members of a binding pair
JP5500423B2 (en) * 2009-12-24 2014-05-21 東洋紡株式会社 Allergy test method
CN113125715B (en) * 2019-12-31 2023-07-28 科美博阳诊断技术(上海)有限公司 Human immunodeficiency virus antibody detection kit and application thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
IL55816A (en) * 1978-10-30 1982-04-30 Ames Yissum Ltd Method for simultaneous immunoassay of several different antibodies and a kit therefor
US4378344A (en) * 1979-09-28 1983-03-29 Ventrex Laboratories, Inc. Method and apparatus for performing multiple, simultaneous in vitro diagnostic tests using a solid phase system
ZA855031B (en) * 1984-08-31 1986-02-26 New York Blood Center Inc Assay for simultaneous detection of antigen and antibody in given serum
GB2181840B (en) * 1985-10-16 1989-11-29 Farmos Group Ltd Method for the immunoassay of a macromolecular analyte
US4772550A (en) * 1986-02-10 1988-09-20 Miles Inc. Heterogeneous specific binding assay employing an aggregatable binding reagent
US4778751A (en) * 1986-05-12 1988-10-18 Diagnostic Products Corporation Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibodies
FR2601455B1 (en) * 1986-07-11 1989-08-04 Stallergenes Lab NEW SOLID-PHASE BIOLOGICAL REAGENTS AND THEIR PROCESS FOR OBTAINING
DE3705686C2 (en) * 1987-02-23 1995-11-30 Boehringer Mannheim Gmbh Methods for the determination of antibodies

Also Published As

Publication number Publication date
JPH02228561A (en) 1990-09-11
EP0379216B1 (en) 1994-06-08
ATE107030T1 (en) 1994-06-15
AU4858290A (en) 1990-07-26
CA2008100A1 (en) 1990-07-20
AU633041B2 (en) 1993-01-21
ES2055172T3 (en) 1994-08-16
DE59005973D1 (en) 1994-07-14
JPH0827284B2 (en) 1996-03-21
EP0379216A1 (en) 1990-07-25

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