AU633041B2 - Method for the determination of immunologically detectable substances - Google Patents

Method for the determination of immunologically detectable substances

Info

Publication number
AU633041B2
AU633041B2 AU48582/90A AU4858290A AU633041B2 AU 633041 B2 AU633041 B2 AU 633041B2 AU 48582/90 A AU48582/90 A AU 48582/90A AU 4858290 A AU4858290 A AU 4858290A AU 633041 B2 AU633041 B2 AU 633041B2
Authority
AU
Australia
Prior art keywords
determination
solid phase
bound
binding
specific binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU48582/90A
Other versions
AU4858290A (en
Inventor
Hubert Bayer
Peter Kirch
Christian Klein
Erhard Kopetzki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE3901638A external-priority patent/DE3901638C2/en
Priority claimed from DE19893924239 external-priority patent/DE3924239A1/en
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of AU4858290A publication Critical patent/AU4858290A/en
Application granted granted Critical
Publication of AU633041B2 publication Critical patent/AU633041B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Abstract

For the determination of immunologically detectable substances by the principle of the heterogeneous immunoassay by incubation with at least two receptors R1 and R2, of which R1 mediates the binding to the solid phase and R2 is a conjugate of a ligand which is able to bind to a substance to be determined and of a label, using a solid phase to which a reaction component capable of specific binding is bound, separation of the solids from the liquid phase and determination of the label in one of the two phases, the solid phase is a material to whose surface are bound partners capable of specific binding, and the sample solution is incubated with a plurality of different receptors R1, where each receptor R1 has both binding sites for the partner which is capable of specific binding and is bound to the solid phase and binding sites for one of the substances to be determined. <IMAGE>
AU48582/90A 1989-01-20 1990-01-19 Method for the determination of immunologically detectable substances Ceased AU633041B2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3901638 1989-01-20
DE3901638A DE3901638C2 (en) 1988-05-25 1989-01-20 Method for determining an immunologically detectable substance and suitable reaction vessel
DE3924239 1989-07-21
DE19893924239 DE3924239A1 (en) 1989-07-21 1989-07-21 Simultaneous heterogeneous immunoassay of several analyte

Publications (2)

Publication Number Publication Date
AU4858290A AU4858290A (en) 1990-07-26
AU633041B2 true AU633041B2 (en) 1993-01-21

Family

ID=25876968

Family Applications (1)

Application Number Title Priority Date Filing Date
AU48582/90A Ceased AU633041B2 (en) 1989-01-20 1990-01-19 Method for the determination of immunologically detectable substances

Country Status (8)

Country Link
EP (1) EP0379216B1 (en)
JP (1) JPH0827284B2 (en)
AT (1) ATE107030T1 (en)
AU (1) AU633041B2 (en)
CA (1) CA2008100A1 (en)
DE (1) DE59005973D1 (en)
DK (1) DK0379216T3 (en)
ES (1) ES2055172T3 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7410802B2 (en) 1999-07-30 2008-08-12 Bioe, Inc. Methods for simultaneously detecting both members of a binding pair

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4211108A1 (en) * 1992-04-03 1993-10-07 Boehringer Mannheim Gmbh Virus solution for use in immunoassays
DE4218257A1 (en) * 1992-06-03 1993-12-09 Behringwerke Ag Method for immunochemical determination of an analyte
GB2269896B (en) * 1992-08-03 1997-03-12 Marconi Gec Ltd Separation method
GB2271634B (en) * 1992-09-18 1997-04-09 Marconi Gec Ltd Separation method
DE4236189A1 (en) * 1992-10-27 1994-04-28 Boehringer Mannheim Gmbh Method for the simultaneous determination of antigens and antibodies
GB2273157A (en) * 1992-11-27 1994-06-08 Marconi Gec Ltd Immunological detection/separation using a plurality of immobilised binding agents
BR9404967A (en) * 1993-04-14 1999-06-15 Int Murex Tech Corp Immunoassay
GB2282884A (en) * 1993-04-14 1995-04-19 Int Murex Tech Corp Immunoassay
JP5500423B2 (en) * 2009-12-24 2014-05-21 東洋紡株式会社 Allergy test method
CN113125715B (en) * 2019-12-31 2023-07-28 科美博阳诊断技术(上海)有限公司 Human immunodeficiency virus antibody detection kit and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2181840A (en) * 1985-10-16 1987-04-29 Farmos Group Ltd Method for the immunoassay of a macromolecular analyte
FR2601455A1 (en) * 1986-07-11 1988-01-15 Stallergenes Lab New biological reactants in solid phase and process for obtaining them

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
IL55816A (en) * 1978-10-30 1982-04-30 Ames Yissum Ltd Method for simultaneous immunoassay of several different antibodies and a kit therefor
US4378344A (en) * 1979-09-28 1983-03-29 Ventrex Laboratories, Inc. Method and apparatus for performing multiple, simultaneous in vitro diagnostic tests using a solid phase system
ZA855031B (en) * 1984-08-31 1986-02-26 New York Blood Center Inc Assay for simultaneous detection of antigen and antibody in given serum
US4772550A (en) * 1986-02-10 1988-09-20 Miles Inc. Heterogeneous specific binding assay employing an aggregatable binding reagent
US4778751A (en) * 1986-05-12 1988-10-18 Diagnostic Products Corporation Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibodies
DE3705686C2 (en) * 1987-02-23 1995-11-30 Boehringer Mannheim Gmbh Methods for the determination of antibodies

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2181840A (en) * 1985-10-16 1987-04-29 Farmos Group Ltd Method for the immunoassay of a macromolecular analyte
FR2601455A1 (en) * 1986-07-11 1988-01-15 Stallergenes Lab New biological reactants in solid phase and process for obtaining them

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7410802B2 (en) 1999-07-30 2008-08-12 Bioe, Inc. Methods for simultaneously detecting both members of a binding pair

Also Published As

Publication number Publication date
AU4858290A (en) 1990-07-26
JPH0827284B2 (en) 1996-03-21
DK0379216T3 (en) 1994-10-31
EP0379216A1 (en) 1990-07-25
ES2055172T3 (en) 1994-08-16
EP0379216B1 (en) 1994-06-08
CA2008100A1 (en) 1990-07-20
DE59005973D1 (en) 1994-07-14
ATE107030T1 (en) 1994-06-15
JPH02228561A (en) 1990-09-11

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