DET0007249MA - - Google Patents

Description

INN REPUBLIC OF GERMANY

Registration date: January 12, 1953 Advertised on October 6, 1955

GERMAN PATENT OFFICE

PATENT APPLICATION

CLASS 6b GROUP I602
T 7249 IVa / 6b

Dr. Walter Karsch and Karl Schoedler, Tornesch (Holst.)

have been named as inventors

Tornescher Hefe-Gesellschaft mb H. _r Tornesch (Holst.)

Process for fermenting sugar solutions to butanol, acetone and ethyl alcohol with the help of butanol bacteria

The invention relates to a method for fermenting sugary liquids to butanol, Acetone and ethyl alcohol with the help of butanol-forming bacteria.

It has long been known to ferment sugary liquids, such as corn mash, molasses solutions, wood and plant hydrolysates, with the help of butanol and acetone-forming bacterial strains.
Such bacterial strains are e.g. B. Clostridium acetobutylicum (Weizmann) and Clostridium felsineum. The breakdown of carbohydrates to butanol, acetone and ethyl alcohol takes place via numerous intermediate stages, which have not yet been fully clarified. However, it has been proven that butyric acid and acetic acid are formed as intermediate products of the fermentation process, which are then converted into butanol and acetone. These acids arise in the first phase of fermentation and reach their maximum in the fermentation liquid after 12 to 20 hours, while in the second phase of fermentation a decrease in acids and an increase in butanol and acetone can be observed. Good butanol and acetone yields depend on the balance between the amount of acetic and butyric acid that occurs in the first fermentation phase.

It has already been proposed to determine the content of acetic acid and calcium acetate in fermentation liquids, which are poor in artificially increasing, namely in the

509 565/7

Claims (2)

T 7249 IVa 16h total fermentation mash to ensure an increased yield of butanol and acetone and a reliable fermentation process. ICs have now found that even without adding caleium acetate for the main fermentation, maximum yields of biitanol and acetone can be achieved if calcium acetate or acetic acid is only added to the previous ones. Jiiitanol-acetone fermentations are carried out in such a way that, starting from a laboratory scale, several, at least two to three preliminary fermentation stages are used (introduction of the main course, with about twenty to forty times the amount of liquid being fermented in the following stage Willows during primary fermentation z. P). If ioo cbm Gäillüssigkeil are set for fermentation, then 5 cbm of fermenting wort are used to inoculate the same, which in turn was inoculated with 250 l wort. So if the addition of calcium acetate can be omitted during the main fermentation without reducing the yields, the result is a particularly economical fermentation process. When adding o. a requirement of 21 kg for the associated pre-fermentation of 5 cbm and 0.25 cbm of fulfillment units. Thus, according to the process of the present invention, 37 () kg of calcium acetate can be saved Sterile molasses solution containing 50 g of reduced sugar is mixed with 10 g of carbonate of lime, 10 g of malt pulp and 10 g of calcium acetate after these substances have been sterilized beforehand inoculated with a fermenting laboratory skin of Clostridium acetobutylicum. The fermenting mash is transferred sterile into a container with 50 liters of the same nutrient solution containing 2.25 kg of reduced sugar after about 1 st ingenious fermentation line, after this mash has been sterilized with 200 g of calcium acetate (- 0.4%, based on the fermentation liquid) has been added. After the onset of vigorous fermentation, which occurs after about jo to i. | Hours is the case, this mash is added to 100 l of a sterile molasses solution containing 40 kg of reduced sugar, 4 kg of carbonate of lime and 4 kg of malt sprouts. No calcium acetate is added to this mash. Fermentation is over after about 36 hours and the mash is subjected to distillation. 11.1 kg of butanol, 6.15 kg of acetone and 1.95 kg of ethanol are obtained. The same molasses solution is fermented in a parallel batch with first and second pre-fermentation in the same way, but also in the pre-fermentation stages without adding calcium acetate. The distillation gives 10.2 kg of butanol, 5.16 kg of acetone and 0.84 kg of ethanol. In the first case the total yield of solvents is 40.4%, based on the sugar used, in the second case only 34.2%. (In the example of the American patent specification 2 233 02T, only 28.2% total yield is achieved.) According to the invention, there is also the possibility of setting a corresponding acetic acid content of the premeshing by adding naturally high acetic acid substrates by adding little or no acetic acid content having fermentation substrate adjusts to the desired acetic acid content. Fermentation substrates with a high acetic acid content are, for example, softwood and beechwood sulphite waste liquors, which can contain up to 20 g acetic acid per liter, and wood sugar spices, whose acetic acid content can go up to 12 g per liter. Pa te ν tanspr ν γ. π f .: 1. Process for fermenting sugary liquids to butanol, acetone and ethyl alcohol with the addition of acetic acid or calcium acetate using pre-fermentation stages, thereby characterized in that only the pre-fermentation stage or stages acetic acid or calcium acetate are added will. 2. The method according to claim 1, characterized in that that the desired acetic acid content of the preliminary fermentation stage or stages by mixing one Fermentation substrate with a high acetic acid content and a fermentation substrate with little or no Acetic acid content is adjusted. Referred publications:
German patent specifications No. 612 535, 629 679. © 509 565/7 9. 55