DE959401C - Process for the production of nutritional solutions for the breeding of micromycetes and other filterable germs - Google Patents
Process for the production of nutritional solutions for the breeding of micromycetes and other filterable germsInfo
- Publication number
- DE959401C DE959401C DES40062A DES0040062A DE959401C DE 959401 C DE959401 C DE 959401C DE S40062 A DES40062 A DE S40062A DE S0040062 A DES0040062 A DE S0040062A DE 959401 C DE959401 C DE 959401C
- Authority
- DE
- Germany
- Prior art keywords
- filterable
- germs
- micromycetes
- breeding
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0002—Fungal antigens, e.g. Trichophyton, Aspergillus, Candida
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Plant Substances (AREA)
Description
Verfahren zur Herstellung von Nährlösungen zur Züchtung von Mikromyceten und anderen filtrierbaren Keimen Zusatz zum Patent 937 248 Es hat sich gezeigt, daß außer dien im Patent 937 21,8 genannten Mikromyceten unter gewissen Voraussetzungen auch andere filtrierbare Keime im Blut des Menschen vorkommen, die sich unter den beschriebenen Wachstumsbedingungen ebenfalls vermehren und eine wirksame Vakzine bilden. Wieweit diese Keime als Erreger schwerer, bisher schlecht angehbarer Krankheiten anzusprechen sind, muß noch geprüft werden. Ebenso wie bei den Viren, die sich nach dem derzeitigen Stand der Forschung an den Grenzen zwischen lebender und toter Materie befinden, bleibt auch hier die Frage offen, wieweit es sich um lebende oder tote Materie handelt, wenn auch alle Anzeichen nach den bist herigenAuffassungen für lebendeMaterie sprechen. Eine nicht unwesentliche Verbesserung des Kulturverfahrens zum Nachweis von filtrierbaren Keimen im Blut des Menschen wird nun dadurch erzielt, daß man einen erheblichen Anteil des Kaliumhydroxyds durch Kaliumbicarbonatersetzt, indem man z. B. eine Lauge mit einem Gehalt von etwa 4°/a Kaliumhydroxyd und 6o-/o- Kaliumbicarbonat benutzt, oder aber de zo°/o-ige Kalilauge durch Einwirkung von Kohlensäure aus der Luft stärker als nach dem Hauptpatent umsetzt.A process for the preparation of culture media for culturing protomycetes and other filterable pathogens addition to Patent 937 248 It has been found that in addition to serving referred to in the patent 937 21.8 protomycetes under certain conditions, other filterable bacteria in human blood occur, which among themselves also multiply the growth conditions described and form an effective vaccine. The extent to which these germs can be addressed as pathogens for serious diseases that have hitherto been difficult to attack has yet to be examined. As with the viruses, which, according to the current state of research, are located at the boundaries between living and dead matter, the question remains open as to how far it is living or dead matter, even if all signs are based on the current conceptions for living matter speak. A not insignificant improvement in the culture process for the detection of filterable germs in human blood is now achieved by replacing a considerable proportion of the potassium hydroxide with potassium bicarbonate by z. B. used a lye with a content of about 4% potassium hydroxide and 6o / o potassium bicarbonate, or de zo% potassium hydroxide solution by the action of carbonic acid from the air more than according to the main patent.
Mit einer so hergestellten und im Kulturversuch als brauchbar erkannten,' Nährlösung gelingt es, nicht nur die relativ großen Mikromyceten, sondern auch andere wesentlich kleinere Keime im Blutplasma des Menschen besonders gut zur Vermehrang zu bringen und mikroskopisch nachzuweisen. Die Größenordnung der letzteren und die typischen Virusformen entsprechen in jeder Hinsicht den Beschreibungen von Elementarkörperchen (s. K o 11 e und H e t s c h : Experimentelle Bakteriologie und Infektionskrankheiten, g. Auflage, 1946, S. 65o; Reiner Müller: Medizinische Mikrobiologie, 3. Auflage, 1946, S. 3o3).With a nutrient solution produced in this way and recognized as useful in a culture experiment, it is possible to multiply not only the relatively large micromycetes but also other much smaller germs in the human blood plasma and to detect them microscopically. The magnitude of the latter and the typical virus forms correspond in every respect to the descriptions of elementary bodies (see K o 11 e and H etsc h : Experimental Bacteriology and Infectious Diseases, g. Edition, 1946, p. 65o; Reiner Müller: Medical Microbiology, 3 . Edition, 1946, p. 3o3).
Beispiel Steril entnommenes und durch. Zentrifugieren (1o Minuten bei looo Umdrehungen) von Erythrocyten und Leukocyten befreites Citratblutplasma wird im Volumenverhältnis 1 : 1 mit einer sterilisierten, leicht mit Sauerstoff angereicherten 1o%igen Lauge, die je nach Reinheit des Kaliumhyd'roxyds 3,5 bis 5% Kaliumhydroxyd und dementsprechend 6,5 bis 5% Kaliumbicarbonat enthält, vermischt, wodurch die Mischung einen pH-Wert von 11 bis 11,5 erhält. Es kann aber auch eine durch Sauerstoff und Kohlensäure hinreichend behandelte zo%@ige Kalilauge zur Anwendung kommen. Die Mischung wird bei einer Temperatur von 30° C und niedriger, z. B. 2o° C, gehalten.Example taken and sterile. Centrifuge (10 minutes at looo revolutions) citrated blood plasma freed from erythrocytes and leukocytes is in a volume ratio of 1: 1 with a sterilized, lightly oxygenated enriched 1o% lye, depending on the purity of the potassium hydroxide 3.5 to Contains 5% potassium hydroxide and accordingly 6.5 to 5% potassium bicarbonate, mixed, giving the mixture a pH of 11 to 11.5. But it can also be a zo% potassium hydroxide solution sufficiently treated with oxygen and carbonic acid for use come. The mixture is at a temperature of 30 ° C and lower, e.g. B. 2o ° C, held.
Claims (3)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DES38993A DE937248C (en) | 1954-05-04 | 1954-05-04 | Process for the production of nutritional solutions for breeding micromycetes |
| DES40062A DE959401C (en) | 1954-05-04 | 1954-07-17 | Process for the production of nutritional solutions for the breeding of micromycetes and other filterable germs |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DES38993A DE937248C (en) | 1954-05-04 | 1954-05-04 | Process for the production of nutritional solutions for breeding micromycetes |
| DES40062A DE959401C (en) | 1954-05-04 | 1954-07-17 | Process for the production of nutritional solutions for the breeding of micromycetes and other filterable germs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE959401C true DE959401C (en) | 1957-03-07 |
Family
ID=25995154
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DES38993A Expired DE937248C (en) | 1954-05-04 | 1954-05-04 | Process for the production of nutritional solutions for breeding micromycetes |
| DES40062A Expired DE959401C (en) | 1954-05-04 | 1954-07-17 | Process for the production of nutritional solutions for the breeding of micromycetes and other filterable germs |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DES38993A Expired DE937248C (en) | 1954-05-04 | 1954-05-04 | Process for the production of nutritional solutions for breeding micromycetes |
Country Status (1)
| Country | Link |
|---|---|
| DE (2) | DE937248C (en) |
-
1954
- 1954-05-04 DE DES38993A patent/DE937248C/en not_active Expired
- 1954-07-17 DE DES40062A patent/DE959401C/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE937248C (en) | 1955-12-29 |
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