DE880786C - Process for the manufacture of an antibiotic - Google Patents

Process for the manufacture of an antibiotic

Info

Publication number
DE880786C
DE880786C DEP37962A DEP0037962A DE880786C DE 880786 C DE880786 C DE 880786C DE P37962 A DEP37962 A DE P37962A DE P0037962 A DEP0037962 A DE P0037962A DE 880786 C DE880786 C DE 880786C
Authority
DE
Germany
Prior art keywords
antibiotic
molds
manufacture
humic acid
nutrient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEP37962A
Other languages
German (de)
Inventor
Hans Dr Glathe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DEP37962A priority Critical patent/DE880786C/en
Application granted granted Critical
Publication of DE880786C publication Critical patent/DE880786C/en
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

Verfahren zur Herstellung eines Antibioticums Es ist bereits bekannt, auf Nährböden, die neben den üblichen Nährsalzen Glucose, Laktose, Malz, Stärke oder Amigen als Kohlenstoffquelle enthalten, Schimmelpilze zu züchten und aus den bewachsenen Nährböden die gebildeten antibiotischen Stoffe zu isolieren. In dieser Weise werden z. B. mit Penicillium citrinum die Herstellung von Citrinin, mit Penicillium chrysogenum die Herstellung von Penicillin oder mit anderen Stämmen die Herstellung anderer Antibiotica durchgeführt.Process for the preparation of an antibiotic It is already known on nutrient media that, in addition to the usual nutrient salts, glucose, lactose, malt, starch or contain amigen as a carbon source to grow and remove molds to isolate the formed antibiotic substances from overgrown culture media. In this Way are z. B. with Penicillium citrinum the production of citrinine, with Penicillium chrysogenum the production of penicillin or with other strains the production other antibiotics performed.

Es wurde nun gefunden, daB sich derartige antibiotische Stoffe besonders vorteilhaft herstellen lassen, wenn man zur Züchtung von Schimmelpilzen huminsäurehaltige Nährböden, wie sie im Patent 836 705 beschrieben sind, benutzt und aus den so erhaltenen Kulturen die antibiotischen Stoffein an sich bekannter Weise isoliert. Das Verfahren hat den besonderen Vorteil, daB die bei der Züchtung bestimmter Schimmelpilze besonders schwierige Reinhaltung der Kulturen von unerwünschten Begleitorganismen hier verhältnismäßig leicht gelingt, da die erwünschten Stämme sich anscheinend besser an die Eigenart des Nährsubstrats anzupassen vermögen als andere Mikroorganismen.It has now been found that such antibiotic substances are particularly useful can be produced advantageously if you use humic acid-containing for the cultivation of molds Culture media, as described in patent 836 705, used and from the thus obtained Cultures the antibiotic substances isolated in a manner known per se. The procedure has the particular advantage that it is particularly important in the cultivation of certain molds It is difficult to keep the cultures clean of undesirable accompanying organisms here in proportion succeeds easily, since the desired trunks seem to better adapt to the idiosyncrasy able to adapt to the nutrient substrate than other microorganisms.

Das gebildete Antibioticum ist durch seine bakterizide Wirkung leicht nachweisbar. Es kann unter Umg -hung einer Reindarstellung zusammen mit den von Pilzen befreiten Nährböden verarbeitet und z. B. in Form von Salben therapeutisch verwertet werden. Derartige Produkte zeigen neben der bakteriziden Wirkung der darin enthaltenen antibiotischen Stoffe gleichzeitig noch die therapeutische Wirkung der darin enthaltenen Huminsäuren. Das gebildete Antibioticuin läßt sich aber auch nach bekannten Verfahren, z. B. Extraktion, Salzbildung, rein darstellen und in dieser hochkonzentrierten Form für therapeutische Zwecke verwenden.The antibiotic formed is light due to its bactericidal effect verifiable. It can be displayed in pure form together with the Fungi-free nutrient media processed and z. B. in the form of ointments therapeutically be recycled. Such products show, in addition to the bactericidal effect, the therein contained antibiotic substances at the same time the therapeutic effect of the in this contained humic acids. The antibiotic formed can also be used according to known methods Procedure, e.g. B. extraction, salt formation, represent pure and highly concentrated in this Use form for therapeutic purposes.

- . Beispiel Ein Nährboden, der auf iooo ccm Gesamtflüssigkeit 8 g Glucose und eine 32 g wasserfreier Huminsäure entsprechende Menge an frisch gefällter, kolloidaler Huminsäure enthält, wird mit den aus dem Moor isolierten Penicillien beimpft und io Tage bei 2o bis 23°C gehalten. Dann wird die Kultur gekühlt und mit Amylacetat extrahiert. Nach der weiteren üblichen Reinigung, z. B. durch wiederholte Rückführung in die wäßrige Phase und anschließende Extraktion mit Amylacetat, sowie durch Überführung in Salzform, erhält man ein Pulver von hervorragender bakterizider Wirkung, insbesondere gegen Streptokokken, Staphylokokken und ähnliche Bakterien. Wird die Kultur von den Schimmelpilzen befreit und eventuell unter Kühlung neutralisiert, so kann der Nährboden ohne Reindarstellung der darin enthaltenen Antibiotica direkt für therapeutische Zwecke verwendet werden, da er die kombinierte Wirkung der Antibiotica und der Huminsäure zeigt.-. Example A culture medium containing 8 g Glucose and an amount of freshly precipitated, containing colloidal humic acid is combined with the penicillia isolated from the peatland inoculated and kept at 20 to 23 ° C for 10 days. Then the culture is cooled and with Amyl acetate extracted. After further usual cleaning, e.g. B. by repeated Recirculation into the aqueous phase and subsequent extraction with amyl acetate, as well by converting it into salt form, a powder of excellent bactericidal properties is obtained Effect, especially against streptococci, staphylococci and similar bacteria. If the culture is freed from the molds and possibly neutralized with cooling, in this way the culture medium can be used directly without the antibiotics contained in it being shown in pure form Can be used for therapeutic purposes as it has the combined effects of antibiotics and the humic acid shows.

Claims (2)

PATENTANSPRÜCHE: i. Verfahren zur Herstellung eines Antibioticums, dadurch gekennzeichnet, daß säureunempfindliche Mikroorganismen, insbesondere Schimmelpilze in huminsäurehaltigen Nährmedien gezüchtet werden und das Antibioticum in bekannter Weise gewonnen wird. PATENT CLAIMS: i. Process for the manufacture of an antibiotic, characterized in that acid-insensitive microorganisms, especially molds can be grown in nutrient media containing humic acid and the antibiotic in known Way is won. 2. Verfahren nach Anspruch i, dadurch gekennzeichnet, daß nach Entfernung der Schimmelpilze die mit Stoffwechselprodukten der Schimmelpilze angereicherte huminsäurehaltige Nährlösung weiterverarbeitet wird.2. The method according to claim i, characterized in that according to Removal of molds enriched with metabolic products of molds nutrient solution containing humic acid is further processed.
DEP37962A 1949-03-27 1949-03-27 Process for the manufacture of an antibiotic Expired DE880786C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEP37962A DE880786C (en) 1949-03-27 1949-03-27 Process for the manufacture of an antibiotic

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEP37962A DE880786C (en) 1949-03-27 1949-03-27 Process for the manufacture of an antibiotic

Publications (1)

Publication Number Publication Date
DE880786C true DE880786C (en) 1953-06-25

Family

ID=7375607

Family Applications (1)

Application Number Title Priority Date Filing Date
DEP37962A Expired DE880786C (en) 1949-03-27 1949-03-27 Process for the manufacture of an antibiotic

Country Status (1)

Country Link
DE (1) DE880786C (en)

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