CH275622A - Process for obtaining antibiotic substances by cultivating microorganisms that produce them. - Google Patents
Process for obtaining antibiotic substances by cultivating microorganisms that produce them.Info
- Publication number
- CH275622A CH275622A CH275622DA CH275622A CH 275622 A CH275622 A CH 275622A CH 275622D A CH275622D A CH 275622DA CH 275622 A CH275622 A CH 275622A
- Authority
- CH
- Switzerland
- Prior art keywords
- antibiotic substances
- produce
- cultivating microorganisms
- obtaining antibiotic
- obtaining
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
Verfahren zur Gewinnung von antibiotischen Stoffen durch Kultivierung von solche erzeugenden Mikroorganismen. Es ist bekannt, Nährböden für die Züch tung von Schimmelpilzen zu verwenden, die neben den üblichen Nährsalzen als Kohlen stoffquelle Glukose, Laktose, Malz oder Stärke enthalten. Aus diesen Nährlösungen können naeli beendeter Kultur die von den Schim- inelpilzen erzeugten antibiotischen Substanzen isoliert werden.
Es wurde nun gefunden, dass derartige antibiotische Stoffe, insbesondere bei Verwen- dunn bestimmter Schimmelpilzarten, sieh auch dann gewinnen lassen, wenn man die Kulti vierung der Mikroorganismen in einem humin- säurehaltigen Nährmedium vornimmt.
(legenstand des Patentes ist somit ein Ver fahren zur Herstellung antibiotischer Stoffe durch Kultivierung von solche erzeugenden IVlikroox-ganisnien, welches dadurch gekenn zeichnet ist, dass man die Kultivierung der 111iki-oorganismen in einem huminsä,urehaltigen Nährmedium vornimmt und dass man die antibiotischen Stoffe aus dem Kulturmedium isoliert. Dieses Verfahren hat.
den besonderen Vorteil, dass die bei der Züchtung bestimmter Seliimmelpilze schwierige Reinhaltung der Kulturen liier verhältnismässig leicht gelingt, da gewisse fremde Organismen sieh der Eigen art der Nährlösung nur schlecht anzupassen pflegen.
Beispiel: Ein huminsäurelialtiger Nährboden wird mit. aus dem Moor isolierten Penicillinen be impft und zehn Tage bei 20 bis 23 C ge halten. Dann wird die Kultur gekühlt und mit Amvlaeetat extrahiert. Nach der weiteren üblichen Reinigung, z. B. durch wiederholte Rückführung in die wässrige Phase und an schliessende Extraktion mit Amylacetat sowie durch Überführung in Salzform, erhält man ein Pulver von hervorragender bakterizider Wirkung, insbesondere gegen Streptokokken, Staphy-lokokken und ähnliche Bakterien.
Process for obtaining antibiotic substances by cultivating microorganisms that produce them. It is known to use culture media for the cultivation of molds that contain glucose, lactose, malt or starch as a carbon source in addition to the usual nutrient salts. Once the culture has ended, the antibiotic substances produced by the mold fungi can be isolated from these nutrient solutions.
It has now been found that antibiotic substances of this type, in particular when certain types of mold are used, can also be obtained if the microorganisms are cultivated in a nutrient medium containing humic acid.
(The status of the patent is thus a process for the production of antibiotic substances by cultivating such producing IVlikroox-ganisnien, which is characterized by the fact that the 111iki-oorganisms are cultivated in a humic acid, uric nutrient medium and that the antibiotic substances are removed isolated from the culture medium.
the particular advantage that it is relatively easy to keep the cultures clean, which is difficult in the cultivation of certain mushrooms, since certain foreign organisms tend to adapt only poorly to the nature of the nutrient solution.
Example: A culture medium suitable for humic acid is used with. inoculated penicillins isolated from the moor and kept at 20 to 23 C for ten days. Then the culture is cooled and extracted with Amvlaeetat. After further usual cleaning, e.g. B. by repeated return to the aqueous phase and subsequent extraction with amyl acetate as well as by conversion into salt form, one obtains a powder with excellent bactericidal action, especially against streptococci, staphylococci and similar bacteria.
Claims (1)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH275622T | 1949-02-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH275622A true CH275622A (en) | 1951-05-31 |
Family
ID=4480325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH275622D CH275622A (en) | 1949-02-25 | 1949-02-25 | Process for obtaining antibiotic substances by cultivating microorganisms that produce them. |
Country Status (1)
| Country | Link |
|---|---|
| CH (1) | CH275622A (en) |
-
1949
- 1949-02-25 CH CH275622D patent/CH275622A/en unknown
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