DE69940338D1 - Vereinfachte methode zur entfernung von freiem protein während der herstellung von protein-polysaccharid-konjugaten und impfstoffen unter verwendung von medien mit limitierter durchlässigkeit - Google Patents
Vereinfachte methode zur entfernung von freiem protein während der herstellung von protein-polysaccharid-konjugaten und impfstoffen unter verwendung von medien mit limitierter durchlässigkeitInfo
- Publication number
- DE69940338D1 DE69940338D1 DE69940338T DE69940338T DE69940338D1 DE 69940338 D1 DE69940338 D1 DE 69940338D1 DE 69940338 T DE69940338 T DE 69940338T DE 69940338 T DE69940338 T DE 69940338T DE 69940338 D1 DE69940338 D1 DE 69940338D1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- vaccines
- restricted
- conjugate
- media
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 10
- 108090000623 proteins and genes Proteins 0.000 title abstract 10
- 229920001282 polysaccharide Polymers 0.000 title abstract 6
- 239000005017 polysaccharide Substances 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 5
- 229960005486 vaccine Drugs 0.000 title abstract 2
- 150000004676 glycans Chemical class 0.000 title 1
- 230000035699 permeability Effects 0.000 title 1
- 238000002360 preparation method Methods 0.000 title 1
- 239000007788 liquid Substances 0.000 abstract 5
- 239000000463 material Substances 0.000 abstract 4
- 239000000203 mixture Substances 0.000 abstract 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 1
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/291—Gel sorbents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
- A61K47/646—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent the entire peptide or protein drug conjugate elicits an immune response, e.g. conjugate vaccines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/265—Adsorption chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
- B01J20/103—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate comprising silica
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Dispersion Chemistry (AREA)
- Epidemiology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Inorganic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7379098P | 1998-02-05 | 1998-02-05 | |
| PCT/US1999/002416 WO1999039739A1 (en) | 1998-02-05 | 1999-02-04 | Simplified method for removing free protein during preparation of protein-polysaccharide conjugates and vaccines using restricted-access media |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE69940338D1 true DE69940338D1 (de) | 2009-03-12 |
Family
ID=22115824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69940338T Expired - Fee Related DE69940338D1 (de) | 1998-02-05 | 1999-02-04 | Vereinfachte methode zur entfernung von freiem protein während der herstellung von protein-polysaccharid-konjugaten und impfstoffen unter verwendung von medien mit limitierter durchlässigkeit |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6284250B1 (enExample) |
| EP (1) | EP1053021B1 (enExample) |
| JP (1) | JP2002502830A (enExample) |
| AT (1) | ATE421339T1 (enExample) |
| AU (1) | AU763349B2 (enExample) |
| CA (1) | CA2320470A1 (enExample) |
| DE (1) | DE69940338D1 (enExample) |
| WO (1) | WO1999039739A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6248334B1 (en) * | 1997-01-08 | 2001-06-19 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Process for preparing conjugate vaccines including free protein and the conjugate vaccines, immunogens, and immunogenic reagents produced by this process |
| FR2806304B1 (fr) * | 2000-03-17 | 2002-05-10 | Aventis Pasteur | Conjugues polysaccharidiques du pneumocoque a usage vaccinal contre le tetanos et la diphterie |
| AU2005209303A1 (en) * | 2004-01-29 | 2005-08-11 | Biosynexus, Inc. | Use of amino-oxy functional groups in the preparation of vaccines conjugates |
| GB0409745D0 (en) * | 2004-04-30 | 2004-06-09 | Chiron Srl | Compositions including unconjugated carrier proteins |
| GB0713880D0 (en) * | 2007-07-17 | 2007-08-29 | Novartis Ag | Conjugate purification |
| US8207298B2 (en) * | 2008-05-01 | 2012-06-26 | Archemix Corp. | Methods of separating biopolymer conjugated molecules from unconjugated molecules |
| WO2011017101A2 (en) | 2009-07-27 | 2011-02-10 | Fina Biosolutions, Llc | Method for producing protein-carbohydrate vaccines reduced in free carbohydrate |
| MX2012006961A (es) | 2009-12-17 | 2012-10-03 | Fina Biosolutions Llc | Reactivos quimicos para la activacion de polisacaridos en la preparacion de vacunas conjugadas. |
| EP2560738B8 (en) | 2010-04-23 | 2019-09-25 | Serum Institute of India Private Limited | Simple method for simultaneous removal of multiple impurities from culture supernatants to ultralow levels |
| JP6360482B2 (ja) | 2012-09-17 | 2018-07-18 | ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ | クロマトグラフィー担体及びデバイス |
| WO2014188313A1 (en) | 2013-05-20 | 2014-11-27 | Shantha Biotechnics Limited | Purification of polysaccharide protein conjugates |
| CN104324708B (zh) * | 2014-11-05 | 2016-08-24 | 华玉叶 | 一种亲水色谱介质的制备方法 |
| EP3288983B1 (en) | 2015-04-28 | 2025-10-29 | Biological E Limited | Method for separation of protein and other impurities from microbial capsular polysaccharides |
| CN114544841B (zh) * | 2020-11-24 | 2024-09-03 | 武汉博沃生物科技有限公司 | 一种采用高效液相色谱法测定肺炎球菌多糖-蛋白结合疫苗中dmap残留量的方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4057685A (en) | 1972-02-02 | 1977-11-08 | Abbott Laboratories | Chemically modified endotoxin immunizing agent |
| US4923978A (en) | 1987-12-28 | 1990-05-08 | E. I. Du Pont De Nemours & Company | Process for purifying nucleic acids |
| US4929560A (en) | 1988-02-03 | 1990-05-29 | Damon Biotech, Inc. | Recovery of tissue plasminogen activator |
| US5277813A (en) | 1988-06-17 | 1994-01-11 | S.A.C. Corporation | Shielded stationary phases |
| US5106966A (en) | 1989-07-10 | 1992-04-21 | University Of Kansas | Use of gel polymer for dna extraction with organic solvents |
| US5153312A (en) | 1990-09-28 | 1992-10-06 | American Cyanamid Company | Oligosaccharide conjugate vaccines |
| US5278284A (en) | 1992-05-14 | 1994-01-11 | Miller Brewing Company | Protein purification method |
| CA2145397C (en) * | 1992-09-24 | 2007-10-30 | Harold J. Jennings | Group b streptococcus type ii and type v polysaccharide-protein conjugate vaccines |
| US5439808A (en) * | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
| DE122009000058I1 (de) * | 1993-09-22 | 2009-12-31 | Jackson H M Found Military Med | Verfahren zur aktivierung von löslichem kohlenhydraten durch verwendung von neuen cyanylierungsreagenzien, zur herstellung von immunogenischen konstrukten |
| EP0814833B1 (en) | 1995-03-22 | 2003-05-28 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Producing immunogenic constructs using soluble carbohydrates activated via organic cyanylating reagents |
| WO1996040242A1 (en) * | 1995-06-07 | 1996-12-19 | Smithkline Beecham Biologicals S.A. | Vaccine comprising a polysaccharide antigen-carrier protein conjugate and free carrier protein |
| AUPN694895A0 (en) | 1995-12-01 | 1996-01-04 | Macquarie Research Limited | Desalting and purification of oligosaccharides and their derivatives |
-
1999
- 1999-02-04 AU AU25829/99A patent/AU763349B2/en not_active Ceased
- 1999-02-04 WO PCT/US1999/002416 patent/WO1999039739A1/en not_active Ceased
- 1999-02-04 EP EP99905732A patent/EP1053021B1/en not_active Expired - Lifetime
- 1999-02-04 CA CA002320470A patent/CA2320470A1/en not_active Abandoned
- 1999-02-04 JP JP2000530236A patent/JP2002502830A/ja not_active Withdrawn
- 1999-02-04 AT AT99905732T patent/ATE421339T1/de not_active IP Right Cessation
- 1999-02-04 US US09/244,178 patent/US6284250B1/en not_active Expired - Lifetime
- 1999-02-04 DE DE69940338T patent/DE69940338D1/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2582999A (en) | 1999-08-23 |
| CA2320470A1 (en) | 1999-08-12 |
| ATE421339T1 (de) | 2009-02-15 |
| EP1053021B1 (en) | 2009-01-21 |
| EP1053021A1 (en) | 2000-11-22 |
| AU763349B2 (en) | 2003-07-17 |
| JP2002502830A (ja) | 2002-01-29 |
| WO1999039739A1 (en) | 1999-08-12 |
| US6284250B1 (en) | 2001-09-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8339 | Ceased/non-payment of the annual fee |