DE69922610D1 - Verfahren zur Kultivierung von Langerhansinsel-Zellen - Google Patents

Verfahren zur Kultivierung von Langerhansinsel-Zellen

Info

Publication number
DE69922610D1
DE69922610D1 DE69922610T DE69922610T DE69922610D1 DE 69922610 D1 DE69922610 D1 DE 69922610D1 DE 69922610 T DE69922610 T DE 69922610T DE 69922610 T DE69922610 T DE 69922610T DE 69922610 D1 DE69922610 D1 DE 69922610D1
Authority
DE
Germany
Prior art keywords
islets
fibroblasts
collected
gel
langerhansinsel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69922610T
Other languages
English (en)
Other versions
DE69922610T2 (de
Inventor
Tai-Wook Yoon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KOREA ISLET TRANSPLANTATION INSTITUTE INC., SEOUL,
Original Assignee
Tai-Wook Yoon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tai-Wook Yoon filed Critical Tai-Wook Yoon
Application granted granted Critical
Publication of DE69922610D1 publication Critical patent/DE69922610D1/de
Publication of DE69922610T2 publication Critical patent/DE69922610T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/585—Integrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Diabetes (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Obesity (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
DE69922610T 1998-10-17 1999-10-18 Verfahren zur Kultivierung von Langerhansinsel-Zellen Expired - Fee Related DE69922610T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1019980043491A KR19990014353A (ko) 1998-10-17 1998-10-17 췌도의 대량 배양증식방법
KR9843491 1998-10-17

Publications (2)

Publication Number Publication Date
DE69922610D1 true DE69922610D1 (de) 2005-01-20
DE69922610T2 DE69922610T2 (de) 2005-12-15

Family

ID=19554380

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69922610T Expired - Fee Related DE69922610T2 (de) 1998-10-17 1999-10-18 Verfahren zur Kultivierung von Langerhansinsel-Zellen

Country Status (7)

Country Link
EP (2) EP1344818A1 (de)
JP (1) JP2000139455A (de)
KR (1) KR19990014353A (de)
AT (1) ATE284952T1 (de)
AU (1) AU775691B2 (de)
CA (1) CA2286530A1 (de)
DE (1) DE69922610T2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6592618B2 (en) * 2000-02-29 2003-07-15 The Brigham And Women's Hospital, Inc. In vitro modification of cardiac valvular xenografts
DE10134667A1 (de) * 2001-07-20 2003-02-06 Neuroprogen Gmbh Leipzig Verfahren zur Herstellung isolierter Zellkulturen, Kulturmedium zur Kultivierung von Zellkulturen und Zellkultur
SI1636348T1 (sl) * 2003-06-20 2010-01-29 Vrije Universiteit Brussel Vub Postopek za pridobivanje beta celic iz eksokrinskih celic trebuĺ ne slinavke
WO2005054450A1 (en) * 2003-12-01 2005-06-16 Vertex Pharmaceuticals Incorporated Compositions comprising fetal liver cells and methods useful for hcv infection
CA2581424A1 (en) * 2004-03-09 2005-09-22 Gang Xu Methods for generating insulin-producing cells
KR100855104B1 (ko) * 2006-06-28 2008-08-29 피더블유제네틱스코리아 주식회사 췌도세포의 장기 보존을 위한 취급방법
US20090060843A1 (en) * 2007-08-31 2009-03-05 Biocrine Ab Non-Invasive In Vivo Imaging and Methods for Treating Type I Diabetes
KR101092411B1 (ko) 2009-07-22 2011-12-09 (주)시지바이오 이식용 동종 피부의 처리 방법 및 그로부터 제조된 동결보존동종피부
WO2011110722A1 (es) * 2010-03-12 2011-09-15 Fundación Progreso Y Salud Método para la proliferación "in vitro" de células procedentes de tejidos de origen endodérmico
KR101327630B1 (ko) * 2012-03-05 2013-11-13 울산대학교 산학협력단 아텔로콜라겐을 이용한 췌도세포 이식용 담체의 제조방법 그리고 이를 이용하여 제조된 인공췌장

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE456164B (sv) * 1980-08-20 1988-09-12 Kjell Nilsson Forfarande for immobilisering, odling och efterfoljande frigoring av animala celler samt mikroberare av gelatin med absorberade animala celler
EP0363125A3 (de) * 1988-10-03 1990-08-16 Hana Biologics Inc. Produkt, enthaltend vermehrungsfähige Bauchspeicheldrüsen-Endokrinezellen, und Verfahren
US5116753A (en) * 1991-07-30 1992-05-26 The Salk Institute For Biological Studies Maintenance of pancreatic islets
US5587309A (en) * 1994-04-29 1996-12-24 The United States Of America As Represented By The Department Of Health And Human Services Method of stimulating proliferation and differentiation of human fetal pancreatic cells ex vivo
AU7443996A (en) * 1995-10-30 1997-05-22 Vivorx, Inc. Method for ex vivo proliferation and differentiation of adult pancreatic islet cells, media useful therefor and uses thereof

Also Published As

Publication number Publication date
ATE284952T1 (de) 2005-01-15
AU5593099A (en) 2000-04-20
AU775691B2 (en) 2004-08-12
CA2286530A1 (en) 2000-04-17
EP0994185A3 (de) 2001-07-25
KR19990014353A (ko) 1999-02-25
DE69922610T2 (de) 2005-12-15
EP0994185A2 (de) 2000-04-19
EP0994185B1 (de) 2004-12-15
JP2000139455A (ja) 2000-05-23
EP1344818A1 (de) 2003-09-17

Similar Documents

Publication Publication Date Title
BR9805900B1 (pt) processo para integrar um fragmento de dna no genoma de uma célula de uma planta manocotiledÈnea.
DE69922610D1 (de) Verfahren zur Kultivierung von Langerhansinsel-Zellen
DE69740002D1 (de) Tierzellen und verfahren für die replikation von influenza viren
DE3686050D1 (de) Vorrichtung zur kultivierung von zellen in vitro.
CA2264270A1 (en) Mass production of paclitaxel by changing the temperature of the medium during the plant cell culture
FI892994A0 (fi) Laite fluoresenssin vahvistamiseksi ja kinetiikka ja menetelmiä laitteen käyttämiseksi
ATE479743T1 (de) Verfahren zur herstellung von zellkulturen aus biologischen präparaten für chemotherapeutische und andere assays
DE69935221D1 (de) Methoden und medium zur in vitro-kultivierung von menschlichen embryos
DE3666458D1 (en) Method and device for the _i(in vitro) culture of plant cells and organs, as well as for the micropropagation and regeneration of complete plants
CA2316739A1 (en) Preparation of cells for production of biologicals
AU8797691A (en) Microspore based selection system and products thereof
DE3880170D1 (de) Kugelfoermiger biokatalysator und verfahren zu seiner herstellung.
KR930018034A (ko) 트레할로오스의 제조방법
AU584774B2 (en) Process for regenerating corn
BR9501136A (pt) Processo para a produção de pilocarpina
WO1987007640A1 (en) Process for preparing neuraminidase
DE3871530D1 (de) Vorrichtung zum kultivieren von ruhenden zellen in einem bioreaktor.
DE68913602T2 (de) Gewebe-Immobilisierungs- und Zellkultursystem und Verfahren zum Anbringen biologisch wirksamer Teile an einem Substrat.
ES381368A1 (es) Un metodo de cultivar un genero de celulas hepaticas epite-liales humanas heteroploides.
ATE313622T1 (de) Verfahren zur züchtung von haftenden tierischen zellen
Prakash Some observations on the distribution and pH dependence of the intestinal alkaline phosphatase of steelhead trout (Salmo gairdnerii gairdnerii)
MY106019A (en) Device for enhancing fluorescence and kinetics and methods of using the device.
ATE47425T1 (de) Verfahren und vorrichtung fuer die -i(in-vitro)kultur von pflanzen-zellen und -organen sowie fuer die mikropropagation und die regeneration von ganzen pflanzen.
MX9707195A (es) Metodo de cultivo celular.
KR900009453A (ko) 생물학적 혐기성 공정을 위한 생물 반응방법

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: KOREA ISLET TRANSPLANTATION INSTITUTE INC., SEOUL,

8339 Ceased/non-payment of the annual fee