DE69632068D1 - Transfektionsverfahren mit hilfe von liposomen - Google Patents

Transfektionsverfahren mit hilfe von liposomen

Info

Publication number
DE69632068D1
DE69632068D1 DE69632068T DE69632068T DE69632068D1 DE 69632068 D1 DE69632068 D1 DE 69632068D1 DE 69632068 T DE69632068 T DE 69632068T DE 69632068 T DE69632068 T DE 69632068T DE 69632068 D1 DE69632068 D1 DE 69632068D1
Authority
DE
Germany
Prior art keywords
liposomes
transfection procedure
transfection
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69632068T
Other languages
English (en)
Other versions
DE69632068T2 (de
Inventor
Paavo Kai J Kinnunen
Tommi Paukku
Satu Lauraeus
Ilpo Huhtaniemi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE69632068D1 publication Critical patent/DE69632068D1/de
Publication of DE69632068T2 publication Critical patent/DE69632068T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/10—Phosphatides, e.g. lecithin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/113—Esters of phosphoric acids with unsaturated acyclic alcohols

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE69632068T 1995-11-21 1996-11-20 Transfektionsverfahren mit hilfe von liposomen Expired - Fee Related DE69632068T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56019995A 1995-11-21 1995-11-21
US560199 1995-11-21
PCT/FI1996/000629 WO1997019184A1 (en) 1995-11-21 1996-11-20 Liposomal transfection method

Publications (2)

Publication Number Publication Date
DE69632068D1 true DE69632068D1 (de) 2004-05-06
DE69632068T2 DE69632068T2 (de) 2004-12-30

Family

ID=24236780

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69632068T Expired - Fee Related DE69632068T2 (de) 1995-11-21 1996-11-20 Transfektionsverfahren mit hilfe von liposomen

Country Status (4)

Country Link
EP (1) EP0871758B1 (de)
AU (1) AU7574996A (de)
DE (1) DE69632068T2 (de)
WO (1) WO1997019184A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3604269A4 (de) 2017-03-29 2021-01-27 Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Anwendung einer verbindung oder eines extraktes aus der traditionellen chinesischen medizin zur herstellung eines nukleinsäureabgabemittels und verwandte produkte daraus
CN111918672B (zh) * 2018-03-29 2023-08-08 中国医学科学院基础医学研究所 人工合成的鞘氨醇衍生物类脂质单体及其递送核酸的用途

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0269720B1 (de) * 1986-06-16 1992-02-19 The Liposome Company, Inc. Induktion von asymmetrie in gefässen
US5279833A (en) * 1990-04-04 1994-01-18 Yale University Liposomal transfection of nucleic acids into animal cells
US5651981A (en) * 1994-03-29 1997-07-29 Northwestern University Cationic phospholipids for transfection

Also Published As

Publication number Publication date
DE69632068T2 (de) 2004-12-30
AU7574996A (en) 1997-06-11
EP0871758A1 (de) 1998-10-21
EP0871758B1 (de) 2004-03-31
WO1997019184A1 (en) 1997-05-29

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee