DE69610410T2 - Verwendung eines Osmolyten zur Verringerung oder Aufhebung von nichtkovalenten Bindungen biologischer Moleküle an inerten Oberflächen - Google Patents

Verwendung eines Osmolyten zur Verringerung oder Aufhebung von nichtkovalenten Bindungen biologischer Moleküle an inerten Oberflächen

Info

Publication number
DE69610410T2
DE69610410T2 DE69610410T DE69610410T DE69610410T2 DE 69610410 T2 DE69610410 T2 DE 69610410T2 DE 69610410 T DE69610410 T DE 69610410T DE 69610410 T DE69610410 T DE 69610410T DE 69610410 T2 DE69610410 T2 DE 69610410T2
Authority
DE
Germany
Prior art keywords
osmolyte
break
biological molecules
reduce
inert surfaces
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69610410T
Other languages
English (en)
Other versions
DE69610410D1 (de
Inventor
Elmar Maier
Igor Ivanov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Application granted granted Critical
Publication of DE69610410D1 publication Critical patent/DE69610410D1/de
Publication of DE69610410T2 publication Critical patent/DE69610410T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
DE69610410T 1996-05-23 1996-05-23 Verwendung eines Osmolyten zur Verringerung oder Aufhebung von nichtkovalenten Bindungen biologischer Moleküle an inerten Oberflächen Expired - Lifetime DE69610410T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP96108278A EP0808906B1 (de) 1996-05-23 1996-05-23 Verwendung eines Osmolyten zur Verringerung oder Aufhebung von nichtkovalenten Bindungen biologischer Moleküle an inerten Oberflächen

Publications (2)

Publication Number Publication Date
DE69610410D1 DE69610410D1 (de) 2000-10-26
DE69610410T2 true DE69610410T2 (de) 2001-05-10

Family

ID=8222814

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69610410T Expired - Lifetime DE69610410T2 (de) 1996-05-23 1996-05-23 Verwendung eines Osmolyten zur Verringerung oder Aufhebung von nichtkovalenten Bindungen biologischer Moleküle an inerten Oberflächen

Country Status (4)

Country Link
EP (1) EP0808906B1 (de)
JP (1) JP4113603B2 (de)
AT (1) ATE196510T1 (de)
DE (1) DE69610410T2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1140632C (zh) * 1997-08-14 2004-03-03 宝酒造株式会社 Dna扩增方法及其试剂盒
JP5187847B2 (ja) 2005-05-06 2013-04-24 ジェン−プローブ・インコーポレーテッド 核酸標的の捕獲方法
JP7096482B2 (ja) * 2017-12-05 2022-07-06 藤倉化成株式会社 感作された不溶性担体粒子を含有する免疫測定試薬の劣化防止手段

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0153875A3 (de) * 1984-03-01 1987-06-24 The State Of Victoria Festphase-Enzymimmunanalyse und Testsatz
DE3717402A1 (de) * 1987-05-23 1988-12-08 Behringwerke Ag Verfahren zur bestimmung von proteinen und mittel dazu
US5047326A (en) * 1988-10-07 1991-09-10 Eastman Kodak Company Immunmological reagent composition and its use in the determination of chlamydial or gonococcal antigens
AU674685B2 (en) * 1992-05-01 1997-01-09 Trustees Of The University Of Pennsylvania, The Fluid handling in microfabricated analytical devices
JP3616394B2 (ja) * 1994-01-31 2005-02-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｄｎａ配列決定のアーティファクトの除去のための方法
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
DE4411588C1 (de) * 1994-03-30 1995-09-28 Deutsches Rheuma Forschungszen Puffer für DNA- und RNA-Polymerase-Reaktionen

Also Published As

Publication number Publication date
EP0808906B1 (de) 2000-09-20
ATE196510T1 (de) 2000-10-15
EP0808906A1 (de) 1997-11-26
JPH1066596A (ja) 1998-03-10
DE69610410D1 (de) 2000-10-26
JP4113603B2 (ja) 2008-07-09

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