DE69535223D1 - METHOD AND DEVICE FOR ELECTROCHEMILUMINESCENCE DETECTION OF MAGNETIC PARTICLES - Google Patents

METHOD AND DEVICE FOR ELECTROCHEMILUMINESCENCE DETECTION OF MAGNETIC PARTICLES

Info

Publication number
DE69535223D1
DE69535223D1 DE69535223T DE69535223T DE69535223D1 DE 69535223 D1 DE69535223 D1 DE 69535223D1 DE 69535223 T DE69535223 T DE 69535223T DE 69535223 T DE69535223 T DE 69535223T DE 69535223 D1 DE69535223 D1 DE 69535223D1
Authority
DE
Germany
Prior art keywords
capture
distribution
magnetic field
active species
magnetic particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69535223T
Other languages
German (de)
Other versions
DE69535223T2 (en
Inventor
B Talley
K Leland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bioveris Corp
Original Assignee
Bioveris Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioveris Corp filed Critical Bioveris Corp
Publication of DE69535223D1 publication Critical patent/DE69535223D1/en
Application granted granted Critical
Publication of DE69535223T2 publication Critical patent/DE69535223T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/66—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence
    • G01N21/69—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light electrically excited, e.g. electroluminescence specially adapted for fluids, e.g. molten metal
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Pyridine Compounds (AREA)
  • Hard Magnetic Materials (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method and apparatus for measuring electrochemiluminescence from a sample composition are described wherein magnetically responsive electrochemiluminescent active species are captured on the electrode with the aid of a capture magnet having a configuration such that the magnetic flux lines (or the magnetic field gradient) of at least one magnetic field source therein are compressed and/or dispersed. This capture magnet improves the distribution of the magnetically responsive electrochemiluminescent active species on the electrode surface and reduces interference with the photomultiplier tube, thereby enhancing the ECL signal and improving sensitivity. The improved capture and distribution also allows for shorter assay times.
DE69535223T 1994-11-10 1995-11-13 Method and apparatus for electrochemiluminescent detection of magnetic particles Expired - Lifetime DE69535223T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/339,237 US5744367A (en) 1994-11-10 1994-11-10 Magnetic particle based electrochemiluminescent detection apparatus and method
US339237 1994-11-10
PCT/US1995/014847 WO1996015440A1 (en) 1994-11-10 1995-11-13 Magnetic particle based electrochemiluminescent detection apparatus and method

Publications (2)

Publication Number Publication Date
DE69535223D1 true DE69535223D1 (en) 2006-10-26
DE69535223T2 DE69535223T2 (en) 2007-09-20

Family

ID=23328109

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69535223T Expired - Lifetime DE69535223T2 (en) 1994-11-10 1995-11-13 Method and apparatus for electrochemiluminescent detection of magnetic particles

Country Status (8)

Country Link
US (2) US5744367A (en)
EP (1) EP0871864B1 (en)
JP (2) JPH10509798A (en)
AT (1) ATE339682T1 (en)
AU (1) AU4501596A (en)
DE (1) DE69535223T2 (en)
ES (1) ES2273339T3 (en)
WO (1) WO1996015440A1 (en)

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Also Published As

Publication number Publication date
JP2006184294A (en) 2006-07-13
ATE339682T1 (en) 2006-10-15
US6133043A (en) 2000-10-17
EP0871864B1 (en) 2006-09-13
WO1996015440A1 (en) 1996-05-23
US5744367A (en) 1998-04-28
AU4501596A (en) 1996-06-06
ES2273339T3 (en) 2007-05-01
DE69535223T2 (en) 2007-09-20
EP0871864A4 (en) 1999-02-10
JPH10509798A (en) 1998-09-22
EP0871864A1 (en) 1998-10-21

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