DE69526364D1 - PATCH CLAMP DEVICE AND TECHNOLOGY WITH HIGH FLOW AND LOW LIQUID VOLUME REQUIREMENTS - Google Patents

PATCH CLAMP DEVICE AND TECHNOLOGY WITH HIGH FLOW AND LOW LIQUID VOLUME REQUIREMENTS

Info

Publication number
DE69526364D1
DE69526364D1 DE69526364T DE69526364T DE69526364D1 DE 69526364 D1 DE69526364 D1 DE 69526364D1 DE 69526364 T DE69526364 T DE 69526364T DE 69526364 T DE69526364 T DE 69526364T DE 69526364 D1 DE69526364 D1 DE 69526364D1
Authority
DE
Germany
Prior art keywords
patch clamp
utilizing
novel
tested
technology
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69526364T
Other languages
German (de)
Other versions
DE69526364T2 (en
Inventor
S Ren-Peter Olesen
Palle Christophersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sophion Bioscience AS
Original Assignee
Sophion Bioscience AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sophion Bioscience AS filed Critical Sophion Bioscience AS
Publication of DE69526364D1 publication Critical patent/DE69526364D1/en
Application granted granted Critical
Publication of DE69526364T2 publication Critical patent/DE69526364T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Computer Hardware Design (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Container, Conveyance, Adherence, Positioning, Of Wafer (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
  • Medicinal Preparation (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

Apparatus for carrying out patch clamp technique utilized in studying the effect of certain materials on ion transfer channels in biological tissue, and more particularly patch clamp apparatus utilizing an autosampler, such as those utilized with HPLC apparatus, to provide high throughput, is disclosed. The invention additionally includes novel microperfusion chamber assemblies capable of utilizing only small amounts of material to be tested and only small amounts of liquid carrier, thereby enabling many tests to be completed in a short period of time. The invention more broadly relates to a novel electrophysiology drug handling and application set up for screening of chemical substances while providing high throughput and requiring only low volume of solutions and samples to be tested. The invention also comprises several novel procedures for utilizing the apparatus of the invention.
DE69526364T 1994-10-28 1995-06-07 PATCH CLAMP DEVICE AND TECHNOLOGY WITH HIGH FLOW AND LOW LIQUID VOLUME REQUIREMENTS Expired - Lifetime DE69526364T2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK125294 1994-10-28
PCT/EP1995/002204 WO1996013721A1 (en) 1994-10-28 1995-06-07 Patch clamp apparatus and technique having high throughput and low fluid volume requirements

Publications (2)

Publication Number Publication Date
DE69526364D1 true DE69526364D1 (en) 2002-05-16
DE69526364T2 DE69526364T2 (en) 2002-11-28

Family

ID=8102743

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69526364T Expired - Lifetime DE69526364T2 (en) 1994-10-28 1995-06-07 PATCH CLAMP DEVICE AND TECHNOLOGY WITH HIGH FLOW AND LOW LIQUID VOLUME REQUIREMENTS

Country Status (12)

Country Link
US (2) US6063260A (en)
EP (1) EP0788600B1 (en)
JP (1) JP3688713B2 (en)
AT (1) ATE216080T1 (en)
AU (1) AU2788695A (en)
CA (1) CA2203886C (en)
DE (1) DE69526364T2 (en)
DK (1) DK0788600T3 (en)
ES (1) ES2171542T3 (en)
PT (1) PT788600E (en)
SI (1) SI0788600T1 (en)
WO (1) WO1996013721A1 (en)

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US20020010550A1 (en) 1998-09-14 2002-01-24 George M. Grass Pharmacokinetic-based drug design tool and method
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US7399599B2 (en) * 2000-07-10 2008-07-15 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
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US20050196746A1 (en) * 2001-03-24 2005-09-08 Jia Xu High-density ion transport measurement biochip devices and methods
US20050058990A1 (en) * 2001-03-24 2005-03-17 Antonio Guia Biochip devices for ion transport measurement, methods of manufacture, and methods of use
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
CA2441366A1 (en) * 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20060029955A1 (en) 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use
US6969489B2 (en) 2001-08-24 2005-11-29 Cytoplex Biosciences Micro array for high throughout screening
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US7465558B2 (en) * 2004-05-12 2008-12-16 Wyeth Perfusion system and apparatus for automated multi-channel patch-clamp recordings utilizing inside-out whole-cell configuration
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Also Published As

Publication number Publication date
ES2171542T3 (en) 2002-09-16
US6117291A (en) 2000-09-12
ATE216080T1 (en) 2002-04-15
AU2788695A (en) 1996-05-23
US6063260A (en) 2000-05-16
JP3688713B2 (en) 2005-08-31
CA2203886A1 (en) 1996-05-09
DE69526364T2 (en) 2002-11-28
EP0788600B1 (en) 2002-04-10
DK0788600T3 (en) 2002-08-05
JPH10509794A (en) 1998-09-22
CA2203886C (en) 2003-09-02
SI0788600T1 (en) 2002-06-30
EP0788600A1 (en) 1997-08-13
WO1996013721A1 (en) 1996-05-09
PT788600E (en) 2002-07-31

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Representative=s name: KUDLEK & GRUNERT PATENTANWAELTE PARTNERSCHAFT, 803