DE69514923T2 - MUTED PENICILLIN G ACYLASE GENES - Google Patents

MUTED PENICILLIN G ACYLASE GENES

Info

Publication number
DE69514923T2
DE69514923T2 DE69514923T DE69514923T DE69514923T2 DE 69514923 T2 DE69514923 T2 DE 69514923T2 DE 69514923 T DE69514923 T DE 69514923T DE 69514923 T DE69514923 T DE 69514923T DE 69514923 T2 DE69514923 T2 DE 69514923T2
Authority
DE
Germany
Prior art keywords
penicillin
acylase
muted
acylases
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69514923T
Other languages
German (de)
Other versions
DE69514923D1 (en
Inventor
Der Laan Metske Van
Johannes Quax
Marina Riemens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM NV filed Critical DSM NV
Publication of DE69514923D1 publication Critical patent/DE69514923D1/en
Application granted granted Critical
Publication of DE69514923T2 publication Critical patent/DE69514923T2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/06—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by desacylation of the substituent in the 6 position

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

New mutant beta -lactam Penicillin G acylases are provided, exhibiting altered substrate specificities. These Penicillin G acylases are obtained by expression of a gene encoding said Penicillin G acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type Penicillin G acylase.
DE69514923T 1994-08-12 1995-08-14 MUTED PENICILLIN G ACYLASE GENES Expired - Fee Related DE69514923T2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP94202314 1994-08-12
PCT/EP1995/003249 WO1996005318A1 (en) 1994-08-12 1995-08-14 Mutated penicillin g acylase genes

Publications (2)

Publication Number Publication Date
DE69514923D1 DE69514923D1 (en) 2000-03-09
DE69514923T2 true DE69514923T2 (en) 2000-11-02

Family

ID=8217100

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69514923T Expired - Fee Related DE69514923T2 (en) 1994-08-12 1995-08-14 MUTED PENICILLIN G ACYLASE GENES

Country Status (10)

Country Link
US (2) US5891703A (en)
EP (1) EP0775210B1 (en)
JP (1) JP4147319B2 (en)
CN (1) CN1159444C (en)
AT (1) ATE189477T1 (en)
AU (1) AU3346295A (en)
DE (1) DE69514923T2 (en)
ES (1) ES2144139T3 (en)
MX (1) MX9701025A (en)
WO (1) WO1996005318A1 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2144139T3 (en) * 1994-08-12 2000-06-01 Dsm Nv GENES OF PENICILLIN G-ACILASE MUTATED.
CA2270400C (en) * 1996-11-05 2010-09-28 Bristol-Myers Squibb Company Mutant penicillin g acylases
EP1315827A1 (en) * 2000-09-08 2003-06-04 Dsm N.V. Method for the preparation of enantiomerically enriched amines
WO2003055998A2 (en) * 2001-12-27 2003-07-10 Dsm Ip Assets B.V. Process for the preparation of a beta-lactam antibiotic with mutated penicillin acylase
JP4113427B2 (en) * 2002-08-16 2008-07-09 富士フイルム株式会社 Photothermographic material
DE10251747A1 (en) * 2002-11-05 2004-05-19 Tuhh-Technologie-Gmbh Genetically modified penicillin amidase and process for its production
WO2004111241A1 (en) * 2003-06-12 2004-12-23 Stichting Voor De Technische Wetenschappen Penicillin acylase
KR100530299B1 (en) * 2003-08-11 2005-11-22 산도즈 게엠베하 Cephalosporin c acylase mutant and method for preparing 7-aca using same
ITMI20040016A1 (en) * 2004-01-12 2004-04-12 Antibiotics S P A CEPHALOSPORIN C ACYLASE
CN101177688B (en) * 2006-11-08 2010-12-01 中国科学院上海生命科学研究院 Mutation penicillin G acylase, recombinant expression plasmid and transformation engineering strains thereof
CZ300467B6 (en) * 2007-01-31 2009-05-27 Mikrobiologický ústav AV CR, v.v.i. Penicillin acylase encoding nucleotide sequence having the size of (2646) bp, new recombinant nucleic acid constructs and recombinant microorganisms carrying this sequence
HUE026181T2 (en) 2008-08-27 2016-05-30 Codexis Inc Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010025287A2 (en) * 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
CN102186972B (en) 2008-08-29 2014-08-20 科德克希思公司 Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
WO2010054319A2 (en) 2008-11-10 2010-05-14 Codexis, Inc. Penicillin-g acylases
CN103642780B (en) 2008-12-23 2017-06-20 中化帝斯曼制药有限公司荷兰公司 Mutant penicillin g acylases
SG178456A1 (en) 2009-08-19 2012-04-27 Codexis Inc Ketoreductase polypeptides for the preparation of phenylephrine
EP2566497B1 (en) 2010-05-04 2015-07-29 Codexis, Inc. Biocatalysts for ezetimibe synthesis
EP2723882B1 (en) 2011-06-23 2019-05-01 Centrient Pharmaceuticals Netherlands B.V. Process for preparing 3'-thiosubstituted cephalosporins employing a penicillin g acylase
EP2723881A2 (en) 2011-06-23 2014-04-30 DSM Sinochem Pharmaceuticals Netherlands B.V. Novel crystalline cefoperazone intermediate
WO2013057196A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the preparation of cefamandole nafate
WO2013057197A1 (en) 2011-10-20 2013-04-25 Dsm Sinochem Pharmaceuticals Netherlands B.V. Process for the formylation of cefamandole
RU2537845C2 (en) * 2012-04-25 2015-01-10 Федеральное государственное бюджетное образовательное учреждение высшего профессиональногообразования "Московский государственный университет имени М.В. Ломоносова " (МГУ) Method of synthesising peptides, including beta-lactam antibiotics with application of penicillin acylase version
CN103667418B (en) * 2013-12-18 2015-03-25 华北制药河北华民药业有限责任公司 High-throughput screening method of beta-lactam antibiotic synthetase with high activity
JP6379531B2 (en) * 2014-03-10 2018-08-29 東洋紡株式会社 Process for producing novel β-amino acid
KR102604503B1 (en) 2015-05-07 2023-11-20 코덱시스, 인코포레이티드 Penicillin-G acylase
WO2017186864A1 (en) 2016-04-27 2017-11-02 Sandoz Ag Enzymatic process for the production of beta-lactam antibiotics in the presence of particulate inoculum
US11643642B2 (en) * 2017-06-27 2023-05-09 Codexis, Inc. Penicillin-g acylases
CN110343734B (en) * 2019-06-14 2021-03-23 浙江工业大学 L-glufosinate-ammonium chemoenzymatic production method
CN111172142B (en) * 2020-02-14 2021-09-28 上海陶宇晟生物技术有限责任公司 Cephalosporin C acylase mutant with high thermal stability

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6121097A (en) * 1984-07-10 1986-01-29 Asahi Chem Ind Co Ltd Preparation of 7-aminocephalosporanic acid and derivative thereof
US4981789A (en) * 1987-03-18 1991-01-01 Merck & Co., Inc. One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives
EP0322032A3 (en) * 1987-12-21 1991-01-30 Merck & Co. Inc. One-step enzymatic conversion of cephalosporin c and derivatives to 7-aminocephalosporanic acid and derivatives
ES2077151T3 (en) * 1990-04-18 1995-11-16 Gist Brocades Nv PENICILLIN G-ACILASE, GENE CODER OF THE SAME AND PRODUCTION PROCEDURE OF THIS ENZYME.
ES2119756T3 (en) * 1990-04-18 1998-10-16 Gist Brocades Nv MUTATED GENES OF BETA LACTAMA ACILASA.
GB9019724D0 (en) * 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase
GB9204439D0 (en) * 1992-02-27 1992-04-15 Fujisawa Pharmaceutical Co A new cephalosporin c acylase
TW400384B (en) * 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase
ES2144139T3 (en) * 1994-08-12 2000-06-01 Dsm Nv GENES OF PENICILLIN G-ACILASE MUTATED.
US5516679A (en) * 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
CA2270400C (en) * 1996-11-05 2010-09-28 Bristol-Myers Squibb Company Mutant penicillin g acylases

Also Published As

Publication number Publication date
CN1159444C (en) 2004-07-28
JP4147319B2 (en) 2008-09-10
WO1996005318A1 (en) 1996-02-22
DE69514923D1 (en) 2000-03-09
ATE189477T1 (en) 2000-02-15
EP0775210B1 (en) 2000-02-02
CN1158638A (en) 1997-09-03
JPH10507072A (en) 1998-07-14
EP0775210A1 (en) 1997-05-28
US6033823A (en) 2000-03-07
US5891703A (en) 1999-04-06
ES2144139T3 (en) 2000-06-01
MX9701025A (en) 1997-05-31
AU3346295A (en) 1996-03-07

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: DSM IP ASSETS B.V., HEERLEN, NL

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee