DE69416879T2 - Identifizierung, kwantifizierung und reinigung von insektiziden proteinen aus bacillus thuringiensis - Google Patents

Identifizierung, kwantifizierung und reinigung von insektiziden proteinen aus bacillus thuringiensis

Info

Publication number
DE69416879T2
DE69416879T2 DE69416879T DE69416879T DE69416879T2 DE 69416879 T2 DE69416879 T2 DE 69416879T2 DE 69416879 T DE69416879 T DE 69416879T DE 69416879 T DE69416879 T DE 69416879T DE 69416879 T2 DE69416879 T2 DE 69416879T2
Authority
DE
Germany
Prior art keywords
kwantification
identification
cleaning
bacillus thuringiensis
insecticidal proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69416879T
Other languages
English (en)
Other versions
DE69416879D1 (de
Inventor
Marianne Pusztai-Carey
Paul Richard Carey
Makoto Yaguchi
Timothy Lessard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
National Research Council of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/102,491 external-priority patent/US5356788A/en
Application filed by National Research Council of Canada filed Critical National Research Council of Canada
Publication of DE69416879D1 publication Critical patent/DE69416879D1/de
Application granted granted Critical
Publication of DE69416879T2 publication Critical patent/DE69416879T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911Bacteria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50Isolated enzymes; Isolated proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/26Conditioning of the fluid carrier; Flow patterns
    • G01N30/28Control of physical parameters of the fluid carrier
    • G01N30/34Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Agronomy & Crop Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69416879T 1990-03-14 1994-06-22 Identifizierung, kwantifizierung und reinigung von insektiziden proteinen aus bacillus thuringiensis Expired - Fee Related DE69416879T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US49345390A 1990-03-14 1990-03-14
US83696792A 1992-02-19 1992-02-19
US08/102,491 US5356788A (en) 1990-03-14 1993-08-05 Isolation, quantitation and purification of insecticidal proteins from Bacillus thuringiensis
PCT/CA1994/000346 WO1995035317A1 (en) 1990-03-14 1994-06-22 Identification, quantitation and purification of insecticidal proteins from bacillus thuringiensis

Publications (2)

Publication Number Publication Date
DE69416879D1 DE69416879D1 (de) 1999-04-08
DE69416879T2 true DE69416879T2 (de) 1999-07-08

Family

ID=27427732

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69416879T Expired - Fee Related DE69416879T2 (de) 1990-03-14 1994-06-22 Identifizierung, kwantifizierung und reinigung von insektiziden proteinen aus bacillus thuringiensis

Country Status (8)

Country Link
EP (1) EP0804474B1 (de)
JP (1) JPH10501410A (de)
AU (1) AU688335B2 (de)
DE (1) DE69416879T2 (de)
DK (1) DK0804474T3 (de)
ES (1) ES2127930T3 (de)
GR (1) GR3030216T3 (de)
WO (1) WO1995035317A1 (de)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0618973B2 (ja) * 1988-01-20 1994-03-16 日本石油化学株式会社 熱可塑性樹脂組成物およびその製造方法
DE3923831A1 (de) * 1989-07-19 1991-01-31 Hartmann & Braun Ag Interferometrische einrichtung
JPH0357941A (ja) * 1989-07-27 1991-03-13 Asahi:Kk 摩擦試験機
JPH03244657A (ja) * 1990-02-22 1991-10-31 Polyplastics Co ポリエステル樹脂組成物及び成形品
JP2928318B2 (ja) * 1990-03-12 1999-08-03 ポリプラスチックス株式会社 ポリエステル樹脂組成物及び成形品
US5356788A (en) * 1990-03-14 1994-10-18 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Isolation, quantitation and purification of insecticidal proteins from Bacillus thuringiensis

Also Published As

Publication number Publication date
GR3030216T3 (en) 1999-08-31
JPH10501410A (ja) 1998-02-10
ES2127930T3 (es) 1999-05-01
DE69416879D1 (de) 1999-04-08
AU688335B2 (en) 1998-03-12
AU7066294A (en) 1996-01-15
EP0804474B1 (de) 1999-03-03
DK0804474T3 (da) 1999-10-04
EP0804474A1 (de) 1997-11-05
WO1995035317A1 (en) 1995-12-28

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee