DE69328524T2 - Exonuklease-Dekontaminierungsverfahren - Google Patents

Exonuklease-Dekontaminierungsverfahren

Info

Publication number
DE69328524T2
DE69328524T2 DE1993628524 DE69328524T DE69328524T2 DE 69328524 T2 DE69328524 T2 DE 69328524T2 DE 1993628524 DE1993628524 DE 1993628524 DE 69328524 T DE69328524 T DE 69328524T DE 69328524 T2 DE69328524 T2 DE 69328524T2
Authority
DE
Germany
Prior art keywords
exonuclease
decontamination process
decontamination
exonuclease decontamination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE1993628524
Other languages
English (en)
Other versions
DE69328524D1 (de
Inventor
George Terrance Walker
Melinda S Fraiser
James L Schram
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of DE69328524D1 publication Critical patent/DE69328524D1/de
Application granted granted Critical
Publication of DE69328524T2 publication Critical patent/DE69328524T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE1993628524 1992-08-17 1993-08-09 Exonuklease-Dekontaminierungsverfahren Expired - Fee Related DE69328524T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US93124192A 1992-08-17 1992-08-17

Publications (2)

Publication Number Publication Date
DE69328524D1 DE69328524D1 (de) 2000-06-08
DE69328524T2 true DE69328524T2 (de) 2000-08-31

Family

ID=25460459

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1993628524 Expired - Fee Related DE69328524T2 (de) 1992-08-17 1993-08-09 Exonuklease-Dekontaminierungsverfahren

Country Status (4)

Country Link
EP (1) EP0585660B1 (de)
JP (1) JPH0757187B2 (de)
CA (1) CA2101119A1 (de)
DE (1) DE69328524T2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2119188A1 (en) * 1993-04-05 1994-10-06 Patricia A. Spears Detection and identification of mycobacteria
US5725831A (en) * 1994-03-14 1998-03-10 Becton Dickinson And Company Nucleic acid amplification apparatus
CA2143365A1 (en) * 1994-03-14 1995-09-15 Hugh V. Cottingham Nucleic acid amplification method and apparatus
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
CA2168712A1 (en) * 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
EP1038974A1 (de) * 1999-03-26 2000-09-27 Mira Diagnostica GmbH Verfahren, Oligonucleotide zum Abbau von in-vitro synthetisierten Nucleinsäuremolekülen
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US8361712B2 (en) 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
CN1656233A (zh) 2001-07-15 2005-08-17 凯克研究生院 利用切割剂扩增核酸片段
CA2492220C (en) 2001-07-15 2014-03-18 Keck Graduate Institute Nucleic acid amplification using nicking agents
ATE431407T1 (de) * 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo Verfahren zur herstellung von dna
AU2003205424B2 (en) * 2002-02-20 2008-11-20 Biomolecular Research Solutions Pty Ltd Removal of nucleic acid contaminants
AUPS064202A0 (en) * 2002-02-20 2002-03-14 Biomolecular Research Solutions Pty Ltd Improved method for the analysis of nucleic acid samples
US20100285537A1 (en) * 2009-04-02 2010-11-11 Fluidigm Corporation Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2663949A1 (fr) * 1990-06-29 1992-01-03 Genset Sa Perfectionnements a un procede d'amplification enzymatique in vitro.

Also Published As

Publication number Publication date
JPH0757187B2 (ja) 1995-06-21
DE69328524D1 (de) 2000-06-08
EP0585660A3 (en) 1994-05-11
JPH06165678A (ja) 1994-06-14
CA2101119A1 (en) 1994-02-18
EP0585660B1 (de) 2000-05-03
EP0585660A2 (de) 1994-03-09

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee