DE69034104D1 - Herstellung von heteropolymeren Proteinen - Google Patents
Herstellung von heteropolymeren ProteinenInfo
- Publication number
- DE69034104D1 DE69034104D1 DE69034104T DE69034104T DE69034104D1 DE 69034104 D1 DE69034104 D1 DE 69034104D1 DE 69034104 T DE69034104 T DE 69034104T DE 69034104 T DE69034104 T DE 69034104T DE 69034104 D1 DE69034104 D1 DE 69034104D1
- Authority
- DE
- Germany
- Prior art keywords
- cells transfected
- beta subunit
- alpha
- production
- cdna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 239000002299 complementary DNA Substances 0.000 abstract 3
- 102000003886 Glycoproteins Human genes 0.000 abstract 1
- 108090000288 Glycoproteins Proteins 0.000 abstract 1
- 108091092195 Intron Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 229940088597 hormone Drugs 0.000 abstract 1
- 239000005556 hormone Substances 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Reproductive Health (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36862889A | 1989-06-20 | 1989-06-20 | |
US368628 | 1989-06-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69034104D1 true DE69034104D1 (de) | 2003-11-06 |
DE69034104T2 DE69034104T2 (de) | 2004-04-08 |
Family
ID=23452053
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69034104T Expired - Lifetime DE69034104T2 (de) | 1989-06-20 | 1990-06-20 | Herstellung von heteropolymeren Proteinen |
DE69031611T Expired - Lifetime DE69031611T2 (de) | 1989-06-20 | 1990-06-20 | Herstellung von heteropolymeren Proteinen |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69031611T Expired - Lifetime DE69031611T2 (de) | 1989-06-20 | 1990-06-20 | Herstellung von heteropolymeren Proteinen |
Country Status (7)
Country | Link |
---|---|
EP (2) | EP0735139B1 (de) |
JP (2) | JP3402604B2 (de) |
AT (2) | ATE251216T1 (de) |
CA (1) | CA2018676C (de) |
DE (2) | DE69034104T2 (de) |
DK (1) | DK0735139T3 (de) |
ES (2) | ES2203653T3 (de) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6060283A (en) * | 1996-06-27 | 2000-05-09 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Genomic DNA encoding human interleukin-18 (IL-18, interferon-γ inducing factor) |
AU2000280019A1 (en) * | 2000-10-06 | 2002-04-22 | Regeneron Pharmaceuticals, Inc. | Polypeptides, methods of making, and uses thereof |
US8318457B2 (en) * | 2007-06-28 | 2012-11-27 | Biogenerix Ag | FSH producing cell clone |
EA019530B1 (ru) | 2008-02-08 | 2014-04-30 | Биодженерикс Аг | Жидкая лекарственная форма фсг |
KR101761168B1 (ko) | 2009-04-01 | 2017-07-25 | 바이오제너릭스 게엠베하 | 재조합 fsh 정제 방법 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3545126A1 (de) * | 1985-12-19 | 1987-06-25 | Boehringer Mannheim Gmbh | Verfahren zur verbesserung der expression von eukaryontenprotein |
GB2197321B (en) * | 1986-09-12 | 1990-10-03 | Genentech Inc | Method and vectors for obtaining continuous production of heterologous protein in a eukaryotic host cell line |
WO1990008528A1 (en) * | 1989-01-11 | 1990-08-09 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | Biologically active synthetic thyrotropin and cloned gene for producing same |
-
1990
- 1990-06-11 CA CA002018676A patent/CA2018676C/en not_active Expired - Lifetime
- 1990-06-20 ES ES96102098T patent/ES2203653T3/es not_active Expired - Lifetime
- 1990-06-20 ES ES90306743T patent/ES2108688T3/es not_active Expired - Lifetime
- 1990-06-20 AT AT96102098T patent/ATE251216T1/de not_active IP Right Cessation
- 1990-06-20 AT AT90306743T patent/ATE159544T1/de not_active IP Right Cessation
- 1990-06-20 EP EP96102098A patent/EP0735139B1/de not_active Expired - Lifetime
- 1990-06-20 EP EP90306743A patent/EP0404546B1/de not_active Expired - Lifetime
- 1990-06-20 DE DE69034104T patent/DE69034104T2/de not_active Expired - Lifetime
- 1990-06-20 DK DK96102098T patent/DK0735139T3/da active
- 1990-06-20 JP JP16256290A patent/JP3402604B2/ja not_active Expired - Fee Related
- 1990-06-20 DE DE69031611T patent/DE69031611T2/de not_active Expired - Lifetime
-
2001
- 2001-03-28 JP JP2001093819A patent/JP3814156B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69031611D1 (de) | 1997-11-27 |
DK0735139T3 (da) | 2004-02-02 |
DE69031611T2 (de) | 1998-05-14 |
EP0404546A3 (de) | 1992-04-15 |
EP0735139A1 (de) | 1996-10-02 |
EP0404546B1 (de) | 1997-10-22 |
JP3402604B2 (ja) | 2003-05-06 |
ES2108688T3 (es) | 1998-01-01 |
EP0404546A2 (de) | 1990-12-27 |
CA2018676A1 (en) | 1990-12-20 |
JP3814156B2 (ja) | 2006-08-23 |
JPH0365194A (ja) | 1991-03-20 |
ES2203653T3 (es) | 2004-04-16 |
EP0735139B1 (de) | 2003-10-01 |
ATE251216T1 (de) | 2003-10-15 |
JP2001299374A (ja) | 2001-10-30 |
ATE159544T1 (de) | 1997-11-15 |
DE69034104T2 (de) | 2004-04-08 |
CA2018676C (en) | 2003-05-06 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: LABORATOIRES SERONO S.A., COINSINS, VAUD, CH |