DE60323690D1 - Verfahren zum nachweis der bindung von nukleinsäure und nukleinsäure bindendem protein mittels monomolekularer fluoreszenzanalyse - Google Patents

Verfahren zum nachweis der bindung von nukleinsäure und nukleinsäure bindendem protein mittels monomolekularer fluoreszenzanalyse

Info

Publication number
DE60323690D1
DE60323690D1 DE60323690T DE60323690T DE60323690D1 DE 60323690 D1 DE60323690 D1 DE 60323690D1 DE 60323690 T DE60323690 T DE 60323690T DE 60323690 T DE60323690 T DE 60323690T DE 60323690 D1 DE60323690 D1 DE 60323690D1
Authority
DE
Germany
Prior art keywords
nucleic acid
binding
detecting
binding protein
fluorescent analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60323690T
Other languages
English (en)
Inventor
Noriko Kato
Naoaki Okamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Olympus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olympus Corp filed Critical Olympus Corp
Application granted granted Critical
Publication of DE60323690D1 publication Critical patent/DE60323690D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE60323690T 2003-04-23 2003-04-23 Verfahren zum nachweis der bindung von nukleinsäure und nukleinsäure bindendem protein mittels monomolekularer fluoreszenzanalyse Expired - Fee Related DE60323690D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2003/005189 WO2004095029A1 (ja) 2003-04-23 2003-04-23 一分子蛍光分析により核酸と核酸結合タンパク質との結合を検出する方法

Publications (1)

Publication Number Publication Date
DE60323690D1 true DE60323690D1 (de) 2008-10-30

Family

ID=33307218

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60323690T Expired - Fee Related DE60323690D1 (de) 2003-04-23 2003-04-23 Verfahren zum nachweis der bindung von nukleinsäure und nukleinsäure bindendem protein mittels monomolekularer fluoreszenzanalyse

Country Status (5)

Country Link
US (1) US20060051805A1 (de)
EP (1) EP1621889B1 (de)
CN (1) CN1860368A (de)
DE (1) DE60323690D1 (de)
WO (1) WO2004095029A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2443842A (en) * 2006-11-17 2008-05-21 Univ Basel Determination of transcription factor binding to DNA
JP2021173705A (ja) * 2020-04-28 2021-11-01 国立大学法人北海道大学 標的ポリペプチドの情報取得方法および試薬キット

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ID20397A (id) * 1997-05-26 1998-12-03 Hoechst Ag Pemurnian kompleks transkripsi tingkat lebih tinggi dari hewan bukan manusia transgenik
JP2001272404A (ja) * 2000-03-27 2001-10-05 Olympus Optical Co Ltd 蛍光相関分光法による抗原抗体反応
JP2002281965A (ja) * 2001-03-26 2002-10-02 Olympus Optical Co Ltd 標的核酸分子の検出方法
AU2002305585A1 (en) * 2001-04-23 2002-11-05 Michael F. Chou Transcription factor network discovery methods
JP2003035714A (ja) * 2001-07-23 2003-02-07 Olympus Optical Co Ltd 蛋白質に対する被検物質の結合能の有無を検出する方法、並びにその方法に使用するための発現ベクターを用いて溶液中で生成された標識蛋白質およびその製造方法

Also Published As

Publication number Publication date
CN1860368A (zh) 2006-11-08
US20060051805A1 (en) 2006-03-09
EP1621889A4 (de) 2006-07-26
EP1621889A1 (de) 2006-02-01
EP1621889B1 (de) 2008-09-17
WO2004095029A1 (ja) 2004-11-04

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee