DE602006021476D1 - Vektor zur effizienten selektion und/oder reifung eines antikörpers und verwendungen davon - Google Patents

Vektor zur effizienten selektion und/oder reifung eines antikörpers und verwendungen davon

Info

Publication number
DE602006021476D1
DE602006021476D1 DE602006021476T DE602006021476T DE602006021476D1 DE 602006021476 D1 DE602006021476 D1 DE 602006021476D1 DE 602006021476 T DE602006021476 T DE 602006021476T DE 602006021476 T DE602006021476 T DE 602006021476T DE 602006021476 D1 DE602006021476 D1 DE 602006021476D1
Authority
DE
Germany
Prior art keywords
antibody
reproduction
vector
efficient selection
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602006021476T
Other languages
English (en)
Inventor
Olga Minenkova
Emiliano Pavoni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Tau Industrie Farmaceutiche Riunite SpA
Tecnogen SpA
Original Assignee
Sigma Tau Industrie Farmaceutiche Riunite SpA
Tecnogen SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Tau Industrie Farmaceutiche Riunite SpA, Tecnogen SpA filed Critical Sigma Tau Industrie Farmaceutiche Riunite SpA
Publication of DE602006021476D1 publication Critical patent/DE602006021476D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3015—Breast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Indexing, Searching, Synchronizing, And The Amount Of Synchronization Travel Of Record Carriers (AREA)
DE602006021476T 2005-12-27 2006-12-27 Vektor zur effizienten selektion und/oder reifung eines antikörpers und verwendungen davon Active DE602006021476D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05028501A EP1803814A1 (de) 2005-12-27 2005-12-27 Methode zur Verbesserung der Antikörperselektionskapazität in einer Phagen-Display-Bibliothek
PCT/IT2006/000876 WO2007074496A2 (en) 2005-12-27 2006-12-27 Vector for efficient selection and/or maturation of an antibody and uses thereof

Publications (1)

Publication Number Publication Date
DE602006021476D1 true DE602006021476D1 (de) 2011-06-01

Family

ID=36177357

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602006021476T Active DE602006021476D1 (de) 2005-12-27 2006-12-27 Vektor zur effizienten selektion und/oder reifung eines antikörpers und verwendungen davon

Country Status (17)

Country Link
US (2) US8003383B2 (de)
EP (2) EP1803814A1 (de)
JP (1) JP5435956B2 (de)
AT (1) ATE506435T1 (de)
AU (1) AU2006329517B2 (de)
CA (1) CA2634962C (de)
CY (1) CY1111505T1 (de)
DE (1) DE602006021476D1 (de)
DK (1) DK1966375T3 (de)
ES (1) ES2362120T3 (de)
HR (1) HRP20110369T1 (de)
IL (1) IL191633A (de)
PL (1) PL1966375T3 (de)
PT (1) PT1966375E (de)
RS (1) RS51866B (de)
SI (1) SI1966375T1 (de)
WO (1) WO2007074496A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1803814A1 (de) 2005-12-27 2007-07-04 SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A. Methode zur Verbesserung der Antikörperselektionskapazität in einer Phagen-Display-Bibliothek
WO2009109572A2 (en) * 2008-03-03 2009-09-11 Ablynx Nv Monovalent phage display of single variable domains
WO2013115425A1 (en) * 2012-01-31 2013-08-08 Scripps Korea Antibody Institute Methods for screening an antibody by one-cycle panning
EP3514175A1 (de) 2012-12-17 2019-07-24 Laboratoire Français du Fractionnement et des Biotechnologies Einsatz von monoklonalen antikörpern für die behandlung von bakteriellen entzündungen und infektionen
PL3294775T3 (pl) 2015-05-12 2021-12-13 Regeneron Pharmaceuticals, Inc. Oznaczanie czystości białka multimerycznego
NZ743713A (en) 2015-12-15 2020-01-31 Gilead Sciences Inc Human immunodeficiency virus neutralizing antibodies
JP7083819B2 (ja) 2016-09-30 2022-06-13 セントレ ナショナル デ ラ レセルシュ シャンティフィク 細胞マーカー
TWI728250B (zh) 2017-06-21 2021-05-21 美商基利科學股份有限公司 靶向hiv gp120及cd3之多特異性抗體
CN111196857B (zh) * 2020-02-05 2023-07-21 杭州贤至生物科技有限公司 一种新型冠状病毒多表位重组抗原及其制备方法
WO2021226249A1 (en) * 2020-05-06 2021-11-11 Sorrento Therapeutics, Inc. Neutralizing antibodies that bind the sars-cov-2 s protein
WO2022032139A1 (en) * 2020-08-07 2022-02-10 Sorrento Therapeutics, Inc. Neutralizing antibodies that bind the sars-cov-2 s protein
WO2022053188A1 (en) 2020-09-11 2022-03-17 Alfasigma S.P.A. NOVEL DNA SEQUENCES ENCODING HUMAN SCFVs ANTIBODIES WITH NEUTRALIZING ACTIVITY AGAINST SARS-CORONAVIRUS-2 AND USES THEREOF

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3663684A (en) * 1970-06-01 1972-05-16 Hoffmann La Roche Carcinoembryonic antigen and diagnostic method using radioactive iodine
EP1026253B2 (de) 1989-03-21 2012-12-19 Vical Incorporated Expression von exogenen Polynukleotidsequenzen in Wirbeltieren
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
JPH0482266A (ja) * 1990-07-24 1992-03-16 Seiko Epson Corp 半導体装置、及びその製造方法
CA2105300C (en) * 1991-03-01 2008-12-23 Robert C. Ladner Process for the development of binding mini-proteins
ATE275198T1 (de) * 1991-12-02 2004-09-15 Medical Res Council Herstellung von antikörpern auf phagenoberflächen ausgehend von antikörpersegmentbibliotheken.
GB9610967D0 (en) * 1996-05-24 1996-07-31 Cambridge Antibody Tech Specific binding members,materials and methods
US6703015B1 (en) 1999-09-03 2004-03-09 Ramot At Tel-Aviv University Ltd. Filamentous bacteriophage displaying an β-amyloid epitope
KR20010079671A (ko) 1998-08-20 2001-08-22 스티븐 에이. 서윈. 엠.디. 재조합 유전자 생성물을 생성시키는 동안 세포독성을조절하는 억제 ｔＲＮＡ의 용도
CA2286301A1 (en) 1999-11-01 2001-05-01 Michael Bruce Zwick The filamentous phage as a multivalent scaffold for coupling synthetic peptides for immunization
ES2370262T3 (es) * 1999-11-15 2011-12-14 Oncothyreon Inc. Análogos del lípido a sintéticos y sus utilizaciones.
TW513852B (en) 2002-01-21 2002-12-11 Nat Chung Cheng Univerity Multiphase oscillator and multiphase oscillation signal generating method
US20040151724A1 (en) * 2002-10-31 2004-08-05 Julia Coronella-Wood Antibody fab fragments specific for breast cancer
US20060292554A1 (en) 2004-05-18 2006-12-28 Genentech, Inc. Major coat protein variants for C-terminal and bi-terminal display
EP1803814A1 (de) 2005-12-27 2007-07-04 SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A. Methode zur Verbesserung der Antikörperselektionskapazität in einer Phagen-Display-Bibliothek
US20130122033A1 (en) 2010-07-02 2013-05-16 Rita De Santis Fimh vaccine against urinary tract infections (uti)

Also Published As

Publication number Publication date
PT1966375E (pt) 2011-06-28
RS51866B (en) 2012-02-29
HRP20110369T1 (hr) 2011-06-30
JP5435956B2 (ja) 2014-03-05
WO2007074496A3 (en) 2008-01-10
CA2634962A1 (en) 2007-07-05
DK1966375T3 (da) 2011-06-20
AU2006329517B2 (en) 2012-09-06
EP1966375B1 (de) 2011-04-20
CY1111505T1 (el) 2015-08-05
IL191633A0 (en) 2008-12-29
IL191633A (en) 2012-04-30
US20080286274A1 (en) 2008-11-20
AU2006329517A1 (en) 2007-07-05
WO2007074496A2 (en) 2007-07-05
ATE506435T1 (de) 2011-05-15
SI1966375T1 (sl) 2011-07-29
PL1966375T3 (pl) 2011-08-31
US8003383B2 (en) 2011-08-23
JP2009521241A (ja) 2009-06-04
EP1803814A1 (de) 2007-07-04
EP1966375A2 (de) 2008-09-10
CA2634962C (en) 2015-03-31
ES2362120T3 (es) 2011-06-28
USRE45968E1 (en) 2016-04-12

Similar Documents

Publication Publication Date Title
DE602006021476D1 (de) Vektor zur effizienten selektion und/oder reifung eines antikörpers und verwendungen davon
NO2017032I1 (no) bezlotoksumab
WO2008022152A3 (en) Optimized antibodies that target cd19
ZA200706036B (en) Anti-IL-12 antibodies, epitopes, compositions, methods and uses
IL225752A0 (en) Antibodies that bind to transforming growth factor b, and methods for their preparation
WO2007063415A3 (en) Methods of producing stable b-lymphocytes
NO344992B1 (no) Anti-GM-CSF antistoffer og anvendelser for disse
NO20054079D0 (no) Hydrokarbongassprosess.
IL187710A0 (en) Anti-trkb monoclonal antibodies and uses thereof
PT2540820T (pt) Meios e métodos para influenciar a establidade de células produtoras de anticorpos
ATE427040T1 (de) Verfahren zur herstellung von nahrungsmitteln und so hergestelltes nahrungsmittel
DE602004025706D1 (de)
WO2004046314A3 (en) Methods for detecting asymmetric dimethylarginine in a biological sample
WO2005049652A3 (en) Methods of generating antibody diversity in vitro
PL1919951T3 (pl) Przeciwciała anty-TNF-alfa oraz ich zastosowania
ATE491851T1 (de) Zur lagerung von schlecht riechenden abwässern konstruiertes gebäude
ATE426046T1 (de) Stoffliche nutzung von biogas
GEP20064010B (en) Method for enhancing plasma jet
ATE520715T1 (de) Anti-heparansulfat-antikörper, verfahren zum nachweis von heparansulfat, und kit zum nachweis von heparansulfat
RU2004115017A (ru) Способ моделирования острого панкреатита
ES1057870Y (es) Valvula de purga de gas electromagnetica.
TH0901002288A (th) การสร้างคลังฟาจที่แสดงแอนติบอดีสายเดี่ยวของมุนษย์แต่ละชนิดบนผิวของฟาจแต่ละอนุภาคและมียีนที่เป็นรหัสของแอนติบอดีนั้นๆ อยู่ในจีโนมของฟาจด้วย (คลังแอนติบอดีของมุนษย์) และการเตรียมแอนติบอดีสายเดี่ยวที่เป็นโปรตีนของมนุษย์ โดยสมบูรณ์ชนิดโมโนโคลนาลและโพลีโคลนาลต่อพิษของงูเห่าไทยด้วยการประยุกต์ใช้คลังฟาจดังกล่าวเป็นเครื่องมือทางชีววิทยา