DE602006020045D1 - MEMBRANE TRANSLATING PEPTIDES - Google Patents
MEMBRANE TRANSLATING PEPTIDESInfo
- Publication number
- DE602006020045D1 DE602006020045D1 DE602006020045T DE602006020045T DE602006020045D1 DE 602006020045 D1 DE602006020045 D1 DE 602006020045D1 DE 602006020045 T DE602006020045 T DE 602006020045T DE 602006020045 T DE602006020045 T DE 602006020045T DE 602006020045 D1 DE602006020045 D1 DE 602006020045D1
- Authority
- DE
- Germany
- Prior art keywords
- membrane
- complexes
- peptide
- nucleic acid
- peptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
A method is selects membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalized nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0515115.4A GB0515115D0 (en) | 2005-07-22 | 2005-07-22 | Peptide characterisation |
| PCT/GB2006/002766 WO2007010293A1 (en) | 2005-07-22 | 2006-07-24 | Membrane-translocating peptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602006020045D1 true DE602006020045D1 (en) | 2011-03-24 |
Family
ID=34976423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602006020045T Active DE602006020045D1 (en) | 2005-07-22 | 2006-07-24 | MEMBRANE TRANSLATING PEPTIDES |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US8557744B2 (en) |
| EP (1) | EP1907544B1 (en) |
| JP (1) | JP5127709B2 (en) |
| CN (1) | CN101258242B (en) |
| AT (1) | ATE498009T1 (en) |
| AU (1) | AU2006271395B2 (en) |
| CA (1) | CA2658491C (en) |
| DE (1) | DE602006020045D1 (en) |
| DK (1) | DK1907544T3 (en) |
| ES (1) | ES2360420T3 (en) |
| GB (1) | GB0515115D0 (en) |
| PT (1) | PT1907544E (en) |
| WO (1) | WO2007010293A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423555B (en) * | 2008-11-14 | 2011-04-20 | 四川大学华西医院 | Membrane penetrating polypeptide for specifically blocking hepatitis B virus assembly and replication |
| KR101095841B1 (en) * | 2009-02-19 | 2011-12-21 | 주식회사 나이벡 | Target Activated Cells/Tissue Translocation Peptide for Impermeable Compound StrategyTACTICS and Use Thereof |
| GB201000352D0 (en) | 2010-01-11 | 2010-02-24 | Isogenica Ltd | Antimicrobial peptides |
| GB201013284D0 (en) | 2010-08-06 | 2010-09-22 | Isogenica Ltd | Scaffold peptides |
| WO2014205518A1 (en) * | 2013-06-26 | 2014-12-31 | Phylogica Limited | Method of monitoring cellular trafficking of peptides |
| JP6415804B2 (en) * | 2013-09-30 | 2018-10-31 | 国立大学法人埼玉大学 | Liposome-binding peptide and method for producing the same |
| CA3057619A1 (en) | 2017-03-22 | 2018-09-27 | Research Corporation Technologies, Inc. | Engineered stable ch2 polypeptides |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6248558B1 (en) * | 1998-03-31 | 2001-06-19 | Vanderbilt University | Sequence and method for genetic engineering of proteins with cell membrane translocating activity |
| PT1078051E (en) | 1998-05-13 | 2008-03-17 | Domantis Ltd | Phage-display system for the selection of correctly folded proteins |
| US6492143B1 (en) | 1998-12-17 | 2002-12-10 | The John Hopkins University | Olfactory receptor expression libraries and methods of making and using them |
| JP2002539839A (en) * | 1999-03-31 | 2002-11-26 | インヴィトロゲン・コーポレーション | Delivery of functional protein sequences by translocating polypeptides |
| US7892532B2 (en) * | 1999-04-30 | 2011-02-22 | Warsaw Orthopedic, In Emory University | Intracellular delivery of osteoinductive proteins and peptides |
| EP1230267B1 (en) | 1999-09-27 | 2006-03-29 | Elan Corporation | Membrane translocating peptide drug delivery system |
| US20030203865A1 (en) * | 2001-04-30 | 2003-10-30 | Pierrot Harvie | Lipid-comprising drug delivery complexes and methods for their production |
| EP2385118A1 (en) * | 2002-09-06 | 2011-11-09 | Isogenica Limited | In vitro peptide expression library |
| DE10256669B4 (en) * | 2002-12-04 | 2006-03-09 | Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle | Mixture of at least two fusion proteins and their preparation and use |
| US20070071677A1 (en) * | 2003-03-10 | 2007-03-29 | Park Yoon J | Non-toxic membrane-translocating peptides |
| WO2005111058A1 (en) * | 2004-04-13 | 2005-11-24 | Medtronic Sofamor Danek, Inc | Intracellular delivery of osteoinductive proteins and peptides |
| EP2392582A3 (en) * | 2003-04-25 | 2012-03-07 | North Carolina State University | Lactobacillus acidophilus nucleic acid sequences encoding cell surface protein homologues and uses therefore |
| US20050032173A1 (en) * | 2003-08-05 | 2005-02-10 | Mauricio Rojas | Fusion proteins with a membrane translocating sequence and methods of using same to inhibit an immune response |
-
2005
- 2005-07-22 GB GBGB0515115.4A patent/GB0515115D0/en not_active Ceased
-
2006
- 2006-07-24 WO PCT/GB2006/002766 patent/WO2007010293A1/en active Application Filing
- 2006-07-24 US US11/996,496 patent/US8557744B2/en not_active Expired - Fee Related
- 2006-07-24 ES ES06765092T patent/ES2360420T3/en active Active
- 2006-07-24 JP JP2008522068A patent/JP5127709B2/en not_active Expired - Fee Related
- 2006-07-24 AU AU2006271395A patent/AU2006271395B2/en not_active Ceased
- 2006-07-24 CA CA2658491A patent/CA2658491C/en not_active Expired - Fee Related
- 2006-07-24 DK DK06765092.9T patent/DK1907544T3/en active
- 2006-07-24 DE DE602006020045T patent/DE602006020045D1/en active Active
- 2006-07-24 PT PT06765092T patent/PT1907544E/en unknown
- 2006-07-24 CN CN2006800267586A patent/CN101258242B/en not_active Expired - Fee Related
- 2006-07-24 AT AT06765092T patent/ATE498009T1/en active
- 2006-07-24 EP EP06765092A patent/EP1907544B1/en not_active Not-in-force
Also Published As
| Publication number | Publication date |
|---|---|
| DK1907544T3 (en) | 2011-05-16 |
| US20080287311A1 (en) | 2008-11-20 |
| CN101258242B (en) | 2013-08-07 |
| CA2658491C (en) | 2013-09-24 |
| AU2006271395B2 (en) | 2011-08-18 |
| ATE498009T1 (en) | 2011-02-15 |
| CA2658491A1 (en) | 2007-01-25 |
| ES2360420T3 (en) | 2011-06-03 |
| EP1907544A1 (en) | 2008-04-09 |
| PT1907544E (en) | 2011-05-03 |
| JP2009502132A (en) | 2009-01-29 |
| CN101258242A (en) | 2008-09-03 |
| AU2006271395A1 (en) | 2007-01-25 |
| US8557744B2 (en) | 2013-10-15 |
| JP5127709B2 (en) | 2013-01-23 |
| WO2007010293A1 (en) | 2007-01-25 |
| EP1907544B1 (en) | 2011-02-09 |
| GB0515115D0 (en) | 2005-08-31 |
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