DE602004027123D1 - Zusammensetzungen und verfahren zur verwendung einer synthetischen dnase i - Google Patents
Zusammensetzungen und verfahren zur verwendung einer synthetischen dnase iInfo
- Publication number
- DE602004027123D1 DE602004027123D1 DE602004027123T DE602004027123T DE602004027123D1 DE 602004027123 D1 DE602004027123 D1 DE 602004027123D1 DE 602004027123 T DE602004027123 T DE 602004027123T DE 602004027123 T DE602004027123 T DE 602004027123T DE 602004027123 D1 DE602004027123 D1 DE 602004027123D1
- Authority
- DE
- Germany
- Prior art keywords
- dna
- dnase
- compositions
- methods
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 abstract 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 abstract 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 abstract 1
- 102000016911 Deoxyribonucleases Human genes 0.000 abstract 1
- 108010053770 Deoxyribonucleases Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 238000002123 RNA extraction Methods 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000005215 recombination Methods 0.000 abstract 1
- 230000006798 recombination Effects 0.000 abstract 1
- 238000003757 reverse transcription PCR Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40402303A | 2003-03-31 | 2003-03-31 | |
| US10/420,345 US7067298B2 (en) | 2003-03-31 | 2003-04-22 | Compositions and methods of using a synthetic Dnase I |
| PCT/US2004/009955 WO2004090114A2 (en) | 2003-03-31 | 2004-03-31 | Compositions and methods of using a synthetic dnase i |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602004027123D1 true DE602004027123D1 (de) | 2010-06-24 |
Family
ID=33161958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602004027123T Expired - Lifetime DE602004027123D1 (de) | 2003-03-31 | 2004-03-31 | Zusammensetzungen und verfahren zur verwendung einer synthetischen dnase i |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US7067298B2 (de) |
| EP (2) | EP1608732B1 (de) |
| AT (1) | ATE467681T1 (de) |
| DE (1) | DE602004027123D1 (de) |
| WO (1) | WO2004090114A2 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2163652B2 (de) | 2002-01-28 | 2023-05-31 | Life Technologies Corporation | Herstellung biologische Rohderivaten unter Einzetsung Proteasen, geeignet für cDNA Synthese |
| ATE387494T1 (de) * | 2002-12-20 | 2008-03-15 | Hoffmann La Roche | Hitzelabile desoxyribonuklease i-varianten |
| EP1727900A4 (de) * | 2004-03-26 | 2008-05-28 | Fujifilm Corp | Verfahren zur selektiven trennung und reinigungvon rna sowie verfahren zur trennung und reinigung von nukleinsäure |
| US20060223072A1 (en) * | 2005-03-31 | 2006-10-05 | Boyes Barry E | Methods of using a DNase I-like enzyme |
| US20060223073A1 (en) * | 2005-03-31 | 2006-10-05 | Boyes Barry E | Methods of using a DNase I-like enzyme |
| US20070141584A1 (en) * | 2005-12-20 | 2007-06-21 | Roberts Douglas N | Methods for assessment of native chromatin on microarrays |
| US20080044851A1 (en) * | 2006-06-02 | 2008-02-21 | Epicentre Technologies | Compositions and methods for removal of DNA from a sample |
| US8725258B2 (en) * | 2006-08-25 | 2014-05-13 | Cardiac Pacemakers, Inc. | Method and apparatus for automated adjustment of arrhythmia detection duration |
| EP2416155A1 (de) | 2006-10-18 | 2012-02-08 | Periness Ltd. | Selektion von Spermien in einer Spermienpopulation zur Verwendung bei der künstlichen Befruchtung |
| US11781127B2 (en) * | 2007-01-16 | 2023-10-10 | Alex Garvin | Homogeneous method to prepare sperm DNA from sexual assault cases |
| US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
| EP2294217A4 (de) * | 2008-05-23 | 2012-10-31 | Life Technologies Corp | Verfahren zur abtrennung von nukleinsäurekontamination von reagentien |
| WO2010071833A1 (en) | 2008-12-19 | 2010-06-24 | Life Technologies Corporation | Proteinase k inhibitors, methods and compositions therefor |
| CA2757951A1 (en) * | 2009-04-10 | 2010-10-14 | Biorad Laboratories, Inc. | Modified dnase compositions and methods of use thereof |
| KR102378465B1 (ko) | 2009-11-02 | 2022-03-28 | 유니버시티 오브 워싱톤 스루 이츠 센터 포 커머셜리제이션 | 치료학적 뉴클레아제 조성물 및 방법 |
| WO2011132089A2 (en) | 2010-02-26 | 2011-10-27 | Dalhousie University | Methods for tissue decellularization |
| GB201101794D0 (en) | 2011-02-02 | 2011-03-16 | Fermentas Uab | Protein production |
| KR102161657B1 (ko) | 2011-04-29 | 2020-10-06 | 유니버시티 오브 워싱톤 스루 이츠 센터 포 커머셜리제이션 | 치료적 뉴클레아제 조성물 및 방법 |
| RU2502803C2 (ru) * | 2011-05-20 | 2013-12-27 | Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") | ПЛАЗМИДА ДЛЯ ЭКСПРЕССИИ В КЛЕТКАХ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, НЕАКТИВНОГО ПРЕДШЕСТВЕННИКА ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНОВ, БАКТЕРИЯ, ПРИНАДЛЕЖАЩАЯ К РОДУ Escherichia, - ПРОДУЦЕНТ НЕАКТИВНОГО ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНА, ПРЕДШЕСТВЕННИК РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА ИЛИ ЕЕ МУТЕИНА, СПОСОБ ПОЛУЧЕНИЯ КОНЪЮГАТОВ ПОЛИЭТИЛЕНГЛИКОЛЯ И РЕКОМБИНАНТНОГО МУТЕИНА ДНКазы I ЧЕЛОВЕКА, ФЕРМЕНТАТИВНО АКТИВНЫЙ КОНЪЮГАТ МУТЕИНА РЕКОМБИНАНТНОЙ ДНКазы I ЧЕЛОВЕКА |
| GB201217405D0 (en) | 2012-09-28 | 2012-11-14 | Fermentas Uab | Protein removal agent |
| ES2759252T3 (es) | 2013-10-31 | 2020-05-08 | Resolve Therapeutics Llc | Fusiones y métodos terapéuticos de nucleasa-albúmina |
| CA2925332C (en) | 2013-11-04 | 2022-07-12 | Lifecell Corporation | Methods of removing alpha-galactose |
| EP3265558B1 (de) | 2015-03-06 | 2020-05-06 | Bio-Rad Laboratories, Inc. | Stabilisierte rna-lösungen |
| US10329623B2 (en) * | 2015-08-28 | 2019-06-25 | Slmp, Llc | Synthetic tissue controls and synthetic tissue microarray controls |
| US10479981B2 (en) | 2015-10-14 | 2019-11-19 | Novozymes A/S | DNase variants |
| AU2020215502A1 (en) | 2019-01-28 | 2021-08-19 | Becton, Dickinson And Company | Oligonucleotide-comprising cellular component-binding reagents and methods of using the same |
| CN110819622A (zh) * | 2019-10-09 | 2020-02-21 | 广州达正生物科技有限公司 | 一种快速提取石蜡组织rna的方法 |
| US20220389397A1 (en) * | 2019-11-08 | 2022-12-08 | Thermo Fisher Scientific Baltics Uab | Deoxyribonuclease variants and uses thereof |
| US11993792B2 (en) | 2021-05-27 | 2024-05-28 | New England Biolabs, Inc. | DNase I variants, compositions, methods, and kits |
| WO2022250676A1 (en) * | 2021-05-27 | 2022-12-01 | New England Biolabs, Inc. | Dnase i variants, compositions, methods, and kits |
| CN114250208B (zh) * | 2021-12-22 | 2023-07-25 | 武汉瀚海新酶生物科技有限公司 | 耐高盐高活性DNase I突变体的制备及应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| US5279823A (en) | 1992-06-08 | 1994-01-18 | Genentech, Inc. | Purified forms of DNASE |
| GB9300686D0 (en) * | 1993-01-15 | 1993-03-03 | Imp Cancer Res Tech | Compounds for targeting |
| US6251648B1 (en) * | 1998-04-03 | 2001-06-26 | Human Genome Sciences, Inc. | Gene encoding human Dnase |
| US6391607B1 (en) | 1996-06-14 | 2002-05-21 | Genentech, Inc. | Human DNase I hyperactive variants |
| CN1152134C (zh) * | 2001-01-19 | 2004-06-02 | 北京华大蛋白质研发中心有限公司 | 一种制备重组脱氧核糖核酸酶i的方法 |
| EP1433842B1 (de) * | 2002-12-18 | 2011-01-05 | Roche Diagnostics GmbH | Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität |
-
2003
- 2003-04-22 US US10/420,345 patent/US7067298B2/en not_active Expired - Lifetime
-
2004
- 2004-03-31 AT AT04749602T patent/ATE467681T1/de not_active IP Right Cessation
- 2004-03-31 WO PCT/US2004/009955 patent/WO2004090114A2/en active Application Filing
- 2004-03-31 EP EP04749602A patent/EP1608732B1/de not_active Expired - Lifetime
- 2004-03-31 EP EP10153371A patent/EP2213741A3/de not_active Withdrawn
- 2004-03-31 DE DE602004027123T patent/DE602004027123D1/de not_active Expired - Lifetime
-
2006
- 2006-02-23 US US11/361,739 patent/US20060228731A1/en not_active Abandoned
-
2011
- 2011-05-09 US US13/103,798 patent/US20110318811A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20110318811A1 (en) | 2011-12-29 |
| WO2004090114A3 (en) | 2006-03-30 |
| US20060228731A1 (en) | 2006-10-12 |
| WO2004090114A2 (en) | 2004-10-21 |
| EP2213741A2 (de) | 2010-08-04 |
| US20040219529A1 (en) | 2004-11-04 |
| EP1608732A2 (de) | 2005-12-28 |
| EP1608732B1 (de) | 2010-05-12 |
| US7067298B2 (en) | 2006-06-27 |
| EP1608732A4 (de) | 2007-09-26 |
| EP2213741A3 (de) | 2010-11-17 |
| ATE467681T1 (de) | 2010-05-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |