DE50106565D1 - Method for identifying cell-specific target structures - Google Patents
Method for identifying cell-specific target structuresInfo
- Publication number
- DE50106565D1 DE50106565D1 DE50106565T DE50106565T DE50106565D1 DE 50106565 D1 DE50106565 D1 DE 50106565D1 DE 50106565 T DE50106565 T DE 50106565T DE 50106565 T DE50106565 T DE 50106565T DE 50106565 D1 DE50106565 D1 DE 50106565D1
- Authority
- DE
- Germany
- Prior art keywords
- detected
- cell
- specific target
- target structures
- identified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000004027 cell Anatomy 0.000 abstract 6
- 239000003153 chemical reaction reagent Substances 0.000 abstract 4
- 238000002372 labelling Methods 0.000 abstract 2
- 239000012528 membrane Substances 0.000 abstract 2
- 238000004458 analytical method Methods 0.000 abstract 1
- 210000000170 cell membrane Anatomy 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Hematology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Identifying cell-specific target structures (A) comprising the automated analysis of reactions between cellular materials and markers, is new. Identifying cell-specific target structures comprises: (i) a reagent solution (Y1), including at least one marker molecule (MM), being deposited automatically on a microscope slide (X1) that supports cells and/or cell membranes derived from a cell or tissue sample; (ii) at least one labeling pattern produced by Y1 is detected automatically; (c) Y1 is removed (before or after step (ii)) and steps (i) and (ii) are repeated with additional reagent solutions (Yn); (iii) the various labeling patterns are combined to form a complex molecular combination pattern (CP) for X1; (iv) steps (i)-(iii) are repeated for additional slides (Xn), carrying different cells and/or membranes, from different samples; (v) at least one difference between CP of X1 and an Xn is detected; (vi) at least one reagent solution that generates the detected difference is identified; (vii) molecules (or molecular complexes) that bind to the MM in the reagent identified in (g) are selected from an homogenate of cells/membranes used to prepare the sample in which the pattern of (vi) was detected; and (viii) biological characterization of the molecules identified in (vii).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE50106565T DE50106565D1 (en) | 2000-03-24 | 2001-03-15 | Method for identifying cell-specific target structures |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10014685A DE10014685B4 (en) | 2000-03-24 | 2000-03-24 | Procedure for the identification of cell-specific target structures |
| DE50106565T DE50106565D1 (en) | 2000-03-24 | 2001-03-15 | Method for identifying cell-specific target structures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE50106565D1 true DE50106565D1 (en) | 2005-07-28 |
Family
ID=7636208
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10014685A Expired - Fee Related DE10014685B4 (en) | 2000-03-24 | 2000-03-24 | Procedure for the identification of cell-specific target structures |
| DE50106565T Expired - Lifetime DE50106565D1 (en) | 2000-03-24 | 2001-03-15 | Method for identifying cell-specific target structures |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10014685A Expired - Fee Related DE10014685B4 (en) | 2000-03-24 | 2000-03-24 | Procedure for the identification of cell-specific target structures |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6924115B2 (en) |
| EP (1) | EP1136822B1 (en) |
| JP (1) | JP2001309799A (en) |
| CN (1) | CN1213300C (en) |
| AT (1) | ATE298426T1 (en) |
| DE (2) | DE10014685B4 (en) |
| ES (1) | ES2244509T3 (en) |
| SG (1) | SG105488A1 (en) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1388734B1 (en) | 2002-08-01 | 2004-03-03 | MTM Laboratories AG | Method for solution based diagnosis |
| US7306926B2 (en) | 1998-07-01 | 2007-12-11 | Mtm Laboratories Ag | Method for detecting carcinomas in a solubilized cervical body sample |
| DE10043470B4 (en) | 2000-09-04 | 2006-01-19 | Mpb Meltec Patent- Und Beteiligungsgesellschaft Mbh | Method for the identification of cell-specific proteins |
| US7306925B2 (en) | 2001-11-09 | 2007-12-11 | Vanderbilt University | Phage antibodies to radiation-inducible neoantigens |
| ATE292793T1 (en) * | 2002-01-22 | 2005-04-15 | Mpb Meltec Patent Und Beteilig | METHOD AND DEVICE FOR SAMPLE PREPARATION FOR ANALYZING BIOLOGICAL SAMPLES |
| DE60300339T2 (en) * | 2003-08-25 | 2006-04-13 | Mtm Laboratories Ag | Method for the detection of carcinomas in solubilized cervical body samples |
| WO2006100033A1 (en) * | 2005-03-21 | 2006-09-28 | Mpb Meltec Patent- Und Beteiligungsgesellschaft Mbh | Method for identifying cell-specific protein combination patterns |
| EP1722231A3 (en) * | 2005-04-27 | 2009-03-11 | MPB MelTec Patent- und Beteiligungsgesellschaft mbH | Method for identification of somatic stem cells |
| DE102006020844A1 (en) * | 2006-05-04 | 2007-11-08 | Adrian Schubert | Process for the functional annotation of molecular networks |
| US9201063B2 (en) * | 2006-11-16 | 2015-12-01 | General Electric Company | Sequential analysis of biological samples |
| US7629125B2 (en) * | 2006-11-16 | 2009-12-08 | General Electric Company | Sequential analysis of biological samples |
| US7741045B2 (en) * | 2006-11-16 | 2010-06-22 | General Electric Company | Sequential analysis of biological samples |
| US8305579B2 (en) | 2006-11-16 | 2012-11-06 | Thomas Pirrie Treynor | Sequential analysis of biological samples |
| DE102007056198B4 (en) * | 2007-11-21 | 2012-01-26 | Niels Grabe | Assessment of the effect of an endogenous or exogenous effect on epithelial tissue by mapping the tissue using labeled histological tissue sections |
| US20090253163A1 (en) * | 2008-04-02 | 2009-10-08 | General Electric Company | Iterative staining of biological samples |
| US9677125B2 (en) * | 2009-10-21 | 2017-06-13 | General Electric Company | Detection of plurality of targets in biological samples |
| US10024796B2 (en) | 2010-10-29 | 2018-07-17 | President And Fellows Of Harvard College | Nucleic acid nanostructure barcode probes |
| US9176032B2 (en) | 2011-12-23 | 2015-11-03 | General Electric Company | Methods of analyzing an H and E stained biological sample |
| US8568991B2 (en) | 2011-12-23 | 2013-10-29 | General Electric Company | Photoactivated chemical bleaching of dyes |
| JP2016513794A (en) | 2013-03-06 | 2016-05-16 | ゼネラル・エレクトリック・カンパニイ | Method for analyzing H & E stained biological sample |
| CN105531377A (en) | 2013-07-30 | 2016-04-27 | 哈佛学院院长及董事 | Quantitative dna-based imaging and super-resolution imaging |
| KR102382892B1 (en) | 2014-03-11 | 2022-04-08 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
| EP3037820A1 (en) | 2014-12-27 | 2016-06-29 | Miltenyi Biotec GmbH | Cell detection methods and reagents having a releasable labelling moiety |
| JP6858744B2 (en) | 2015-08-07 | 2021-04-14 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Ultra-resolution imaging of protein-protein interactions |
| CA3043639A1 (en) | 2016-12-09 | 2018-06-14 | Ultivue, Inc. | Improved methods for multiplex imaging using labeled nucleic acid imaging agents |
| EP3336546B1 (en) | 2016-12-13 | 2020-07-01 | Miltenyi Biotec B.V. & Co. KG | Reversible cell labelling with conjugates having two releasable binding sites |
| EP3489684B1 (en) | 2017-11-27 | 2024-06-26 | Miltenyi Biotec B.V. & Co. KG | Method for photobleaching stained cells |
| EP3953489A1 (en) | 2019-04-12 | 2022-02-16 | Miltenyi Biotec B.V. & Co. KG | Conjugates having an enzymatically releasable detection moiety and a barcode moiety |
| WO2020216439A1 (en) | 2019-04-23 | 2020-10-29 | Miltenyi Biotec B.V. & Co. KG | Reversible cell detection with conjugates having a linker for increased fluorescent brightness and an enzymatically releasable fluorescent moiety |
| WO2021094356A1 (en) | 2019-11-11 | 2021-05-20 | Miltenyi Biotec B.V. & Co. KG | Serial multichannel microscopy |
| EP3916393B1 (en) | 2020-05-27 | 2023-01-25 | Miltenyi Biotec B.V. & Co. KG | Method for detection of cells by repetitive staining and destaining |
| US20210403992A1 (en) | 2020-06-29 | 2021-12-30 | Miltenyi Biotec B.V. & Co. KG | METHOD COMBINING SINGLE CELL GENE EXPRESSION MAPPING AND TARGETED RNA OR c-DNA SEQUENCING USING PADLOCK OLIGONUCLEOTIDES COMPRISING A BARCODE REGION |
| CN116368169A (en) | 2020-07-16 | 2023-06-30 | 美天施生物科技有限两合公司 | Fluorescent dyes comprising pi-conjugated 1,1' -binaphthyl-based polymers |
| EP4204809A1 (en) | 2020-08-27 | 2023-07-05 | Miltenyi Biotec B.V. & Co. KG | Releasable reagents for cell separation |
| EP4211471A1 (en) | 2020-09-07 | 2023-07-19 | Miltenyi Biotec B.V. & Co. KG | Conjugates having an enzymatically releasable detection moiety and a barcode moiety |
| CN114854838A (en) | 2021-02-04 | 2022-08-05 | 美天施生物科技有限两合公司 | Method for combining in-vitro sequencing and in-situ sequencing of target RNA or cDNA |
| US20230138285A1 (en) | 2021-11-04 | 2023-05-04 | Miltenyi Biotec B.V. & Co. KG | Bright and releasable labels for cell staining based on the conjugates with several sites of fluorophore release |
| US20230228745A1 (en) | 2022-01-17 | 2023-07-20 | Miltenyi Biotec B.V. & Co. KG | Water-soluble ii-conjugated fluorescent 1,1 -binaphthyl-based polymers with tuneable absorption |
| US20230228746A1 (en) | 2022-01-17 | 2023-07-20 | Miltenyi Biotec B.V. & Co. KG | Water-soluble M-conjugated fluorescent 1,1-binaphthyl-based tandem polymers |
| EP4257702A1 (en) | 2022-04-07 | 2023-10-11 | Miltenyi Biotec B.V. & Co. KG | Method combining in situ target capture and spatial unique molecular identifier (sumi) identification with in vitro sequencing for high density spatial multiomics |
| US20230383343A1 (en) | 2022-05-30 | 2023-11-30 | Miltenyi Biotec B.V. & Co. KG | Method Combining In Situ Target Amplification and Spatial Unique Molecular Identifier (SUMI) Identification Using RT-PCR |
| EP4343326A1 (en) | 2022-09-20 | 2024-03-27 | Miltenyi Biotec B.V. & Co. KG | Biological conjugates having an photogenerated acidic or basic releasable detection moiety on top of biological tissues |
| EP4361286A1 (en) | 2022-10-25 | 2024-05-01 | Miltenyi Biotec B.V. & Co. KG | Method for determining the spatial localisation of rna strands on a surface |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340565C (en) * | 1989-06-29 | 1999-05-25 | Thomas B. Okarma | Device and process for cell capture and recovery |
| US5256542A (en) * | 1992-03-09 | 1993-10-26 | Tanox Biosystems, Inc. | Selecting low frequency antigen-specific single B lymphocytes with correction for background noise |
| US5275933A (en) * | 1992-09-25 | 1994-01-04 | The Board Of Trustees Of The Leland Stanford Junior University | Triple gradient process for recovering nucleated fetal cells from maternal blood |
| US6017719A (en) * | 1994-06-14 | 2000-01-25 | Nexell Therapeutics, Inc. | Positive and positive/negative cell selection mediated by peptide release |
| EP0810428B1 (en) * | 1996-05-29 | 2004-04-28 | Walter Dr. Schubert | Automated device and method for measuring and identifying molecules or fragments thereof |
| DE19709348C2 (en) * | 1996-05-29 | 1999-07-01 | Schubert Walter Dr Md | Automatic multi-epitope ligand mapping process |
| EP0932882B1 (en) * | 1996-10-25 | 2003-05-02 | Peter Mose Larsen | Proteome analysis for characterization of up- and down-regulated proteins in biological samples |
-
2000
- 2000-03-24 DE DE10014685A patent/DE10014685B4/en not_active Expired - Fee Related
-
2001
- 2001-03-14 US US09/808,224 patent/US6924115B2/en not_active Expired - Fee Related
- 2001-03-15 ES ES01106571T patent/ES2244509T3/en not_active Expired - Lifetime
- 2001-03-15 EP EP01106571A patent/EP1136822B1/en not_active Expired - Lifetime
- 2001-03-15 DE DE50106565T patent/DE50106565D1/en not_active Expired - Lifetime
- 2001-03-15 AT AT01106571T patent/ATE298426T1/en not_active IP Right Cessation
- 2001-03-20 SG SG200101723A patent/SG105488A1/en unknown
- 2001-03-23 CN CNB011097205A patent/CN1213300C/en not_active Expired - Fee Related
- 2001-03-23 JP JP2001086054A patent/JP2001309799A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1136822B1 (en) | 2005-06-22 |
| SG105488A1 (en) | 2004-08-27 |
| US6924115B2 (en) | 2005-08-02 |
| JP2001309799A (en) | 2001-11-06 |
| ES2244509T3 (en) | 2005-12-16 |
| DE10014685A1 (en) | 2001-10-31 |
| CN1334463A (en) | 2002-02-06 |
| CN1213300C (en) | 2005-08-03 |
| ATE298426T1 (en) | 2005-07-15 |
| EP1136822A3 (en) | 2002-05-02 |
| EP1136822A2 (en) | 2001-09-26 |
| US20010039023A1 (en) | 2001-11-08 |
| DE10014685B4 (en) | 2004-07-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |