CA2341421A1 - Removal of embedding media from biological samples and cell conditioning on automated staining instruments - Google Patents

Removal of embedding media from biological samples and cell conditioning on automated staining instruments Download PDF

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Publication number
CA2341421A1
CA2341421A1 CA002341421A CA2341421A CA2341421A1 CA 2341421 A1 CA2341421 A1 CA 2341421A1 CA 002341421 A CA002341421 A CA 002341421A CA 2341421 A CA2341421 A CA 2341421A CA 2341421 A1 CA2341421 A1 CA 2341421A1
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CA
Canada
Prior art keywords
biological samples
embedding media
biological sample
removal
cell conditioning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002341421A
Other languages
French (fr)
Other versions
CA2341421C (en
Inventor
Kimberly Christensen
Ethel Macrea
Noemi Sebastiao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems, Inc.
Kimberly Christensen
Ethel Macrea
Noemi Sebastiao
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/259,240 external-priority patent/US6296809B1/en
Application filed by Ventana Medical Systems, Inc., Kimberly Christensen, Ethel Macrea, Noemi Sebastiao filed Critical Ventana Medical Systems, Inc.
Priority claimed from PCT/US1999/020353 external-priority patent/WO2000014507A1/en
Publication of CA2341421A1 publication Critical patent/CA2341421A1/en
Application granted granted Critical
Publication of CA2341421C publication Critical patent/CA2341421C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

The present invention provides an automated for removing or etching embedding media by exposing the biological samples for use in histochemical or cytochemical staining procedures without the dependence on organic solvents. The method comprises a biological sample slide in contact with a thermal platform, with or without heat, and with or without a fluid, to facilitate removal or etching of the embedding media from the biological sample. The present invention also provides an automated method for cell conditioning biological samples wherein the cells are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The method comprises a biological sample slide in contact with a thermal platform, with or without heat, and with or without a fluid, to facilitate molecular access to cells and cell constituents within the biological sample.
CA002341421A 1998-09-03 1999-09-03 Removal of embedding media from biological samples and cell conditioning on automated staining instruments Expired - Lifetime CA2341421C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US9901898P 1998-09-03 1998-09-03
US60/099,018 1998-09-03
US09/259,240 US6296809B1 (en) 1998-02-27 1999-02-26 Automated molecular pathology apparatus having independent slide heaters
USPCT/US99/04181 1999-02-26
PCT/US1999/004181 WO1999044030A1 (en) 1998-02-27 1999-02-26 Automated molecular pathology apparatus having independent slide heaters
US09/259,240 1999-02-26
PCT/US1999/020353 WO2000014507A1 (en) 1998-09-03 1999-09-03 Removal of embedding media from biological samples and cell conditioning on automated staining instruments

Publications (2)

Publication Number Publication Date
CA2341421A1 true CA2341421A1 (en) 2000-03-16
CA2341421C CA2341421C (en) 2007-04-10

Family

ID=26795388

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002341421A Expired - Lifetime CA2341421C (en) 1998-09-03 1999-09-03 Removal of embedding media from biological samples and cell conditioning on automated staining instruments

Country Status (2)

Country Link
JP (1) JP2005221511A (en)
CA (1) CA2341421C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114878289A (en) * 2022-07-12 2022-08-09 北京大学 Preparation method of low-temperature-resistant and low-salt-resistant cyanobacteria phycobilisome sample

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2008038813A1 (en) 2006-09-28 2010-01-28 株式会社島津製作所 Deparaffinization of paraffin-embedded specimens and analysis of paraffin-embedded specimens
EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
JP2020134323A (en) * 2019-02-20 2020-08-31 サクラ精機株式会社 Tissue piece processor

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114878289A (en) * 2022-07-12 2022-08-09 北京大学 Preparation method of low-temperature-resistant and low-salt-resistant cyanobacteria phycobilisome sample
CN114878289B (en) * 2022-07-12 2022-09-27 北京大学 Preparation method of low-temperature-resistant and low-salt-resistant cyanobacteria phycobilisome sample

Also Published As

Publication number Publication date
CA2341421C (en) 2007-04-10
JP2005221511A (en) 2005-08-18

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Effective date: 20190903