DE3937892C2 - - Google Patents

Info

Publication number
DE3937892C2
DE3937892C2 DE3937892A DE3937892A DE3937892C2 DE 3937892 C2 DE3937892 C2 DE 3937892C2 DE 3937892 A DE3937892 A DE 3937892A DE 3937892 A DE3937892 A DE 3937892A DE 3937892 C2 DE3937892 C2 DE 3937892C2
Authority
DE
Germany
Prior art keywords
enzyme
reactor
membrane
contact
ultrafiltration membrane
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3937892A
Other languages
German (de)
Other versions
DE3937892A1 (en
Inventor
Udo 5170 Juelich De Kragl
Joerg 4006 Erkrath De Peters
Christian Prof. 5170 Juelich De Wandrey
Maria-Regina Prof. 5162 Niederzier De Kula
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Forschungszentrum Juelich GmbH
Original Assignee
Forschungszentrum Juelich GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Juelich GmbH filed Critical Forschungszentrum Juelich GmbH
Priority to DE3937892A priority Critical patent/DE3937892A1/en
Priority to CH3518/90A priority patent/CH679676A5/de
Publication of DE3937892A1 publication Critical patent/DE3937892A1/en
Application granted granted Critical
Publication of DE3937892C2 publication Critical patent/DE3937892C2/de
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters

Description

Die Erfindung bezieht sich auf einen Enzymmembran­ reaktor mit einer Reaktionszelle, die von einer Ultrafiltrationsmembran mit Rückhaltevermögen für Enzyme abgeschlossen ist, über die der Reaktorab­ lauf erfolgt.The invention relates to an enzyme membrane reactor with a reaction cell from a Ultrafiltration membrane with retention capacity for Enzymes through which the reactor is shut off run takes place.

Enzymmembranreaktoren sind für die kontinuierliche Durchführung enzymkatalysierter Umsetzungen von großem Interesse. Die Verhaltensweise solcher Bio­ katalysatoren ist je nach Eigenart unterschiedlich und wird empfindlich durch die umgebenden Medien beeinflußt.Enzyme membrane reactors are for continuous Implementation of enzyme-catalyzed reactions of great interest. The behavior of such bio Catalysts vary depending on their particularity and becomes sensitive to the surrounding media influenced.

Es hat sich nun gezeigt, daß nicht nur das umgeben­ de Medium selbst, sondern auch das Wandmaterial des Reaktors eine Rolle bei der Desaktivierung der im Reaktor enthaltenen Enzyme spielt.It has now been shown that not only that surrounds de medium itself, but also the wall material of the Reactor plays a role in the deactivation of the Reactor contained enzymes plays.

Üblicherweise werden solche Enzymmembranreaktoren aus Stahl, insbesondere Edelstahl, gefertigt. Für Demonstrationszwecke wurden jedoch auch bereits Zellen aus durchsichtigen Kunststoff (insbesondere Plexiglas) gezeigt.Such enzyme membrane reactors are usually used made of steel, especially stainless steel. For However, demonstration purposes have also been used Clear plastic cells (especially Plexiglass).

Es wurde nun gefunden, daß die Desaktivierungsge­ schwindigkeit insbesondere von Aldolasen besonders gering ist, wenn der Reaktor aus einem Kunststoff mit glatter hydrophober Oberfläche besteht, dessen chemische Zusammensetzung möglichst frei von Kompo­ nenten ist, die zu Störungen in der Proteinstruktur des Enzyms führen können.It has now been found that the deactivation gene speed especially of aldolases in particular is low if the reactor is made of a plastic  with a smooth hydrophobic surface, whose chemical composition as free of compo as possible is leading to disturbances in the protein structure of the enzyme can lead.

Der daraufhin entwickelte erfindungsgemäße Enzymmem­ branreaktor der eingangs genannten Art ist dadurch gekennzeichnet, daß die mit enzymhaltigem Reaktions­ medium in Kontakt kommenden Flächen - abgesehen von der Ultrafiltrationsmembran - aus einem thermopla­ stischen Polymerisat bestehen, das frei von -C-S- oder -C-O-Gruppen ist.The enzyme membrane according to the invention then developed branch reactor of the type mentioned is characterized in that with enzyme-containing reaction medium coming into contact - apart from the ultrafiltration membrane - from a thermopla consist polymeric that is free of -C-S- or -C-O groups.

Vorzugsweise ist dabei das Polymerisat aus vinyli­ schem Monomeren der FormelThe polymer is preferably vinyli cal monomers of the formula

gebildet, bei denen R für H, CH3, Cl oder C6H5 steht.formed in which R is H, CH 3 , Cl or C 6 H 5 .

Die beigefügten Zeichnungen dienen zur weiteren Ver­ anschaulichung der Erfindung; es zeigen:The accompanying drawings serve for further ver illustration of the invention; show it:

Fig. 1 schematisch den Aufbau eines EMR im Schnitt; Figure 1 schematically shows the structure of an EMR in section.

Fig. 2 eine gebaute EMR-Zelle in Explosions­ darstellung; Figure 2 is a built EMR cell in an exploded view.

Fig. 3 den Aktivitätsverlauf einer Aldolase in Abhängigkeit von der Zeit bei Anwe­ senheit unterschiedlicher Materialien. Fig. 3 shows the activity profile of an aldolase as a function of time when different materials are present.

Gemäß Fig. 1 umfaßt ein EMR 1 eine durch eine Ul­ trafiltrationsmembran 2 abgeschlossene Reaktionszel­ le 3, in die über ein oder mehrere Zuläufe 4 Sub­ strate eingespeist werden, während über 5 der Reak­ torablauf abgeht. In der Zelle 3 befindet sich die Reaktionsflüssigkeit mit darin löslichem Enzym 6, die mittels eines Magnetrührers 7 bewegt wird.Referring to FIG. 1, an EMR 1 comprises a trafiltrationsmembran by a Ul 2 completed Reaktionszel le 3, in which via one or more feed streams are fed 4 sub strate, while 5 of the reac torablauf going on. In the cell 3 there is the reaction liquid with the enzyme 6 soluble therein, which is moved by means of a magnetic stirrer 7 .

Eine praktische Ausführungsart mit Flachmembran ist in Fig. 2 dargestellt, gemäß der die Zelle zwischen Endflanschen 8, 8′ gebildet wird, zwischen denen die Seitenwand sowie die Membran samt Dichtringen eingespannt wird.A practical embodiment with flat membrane is shown in Fig. 2, according to which the cell is formed between end flanges 8 , 8 ', between which the side wall and the membrane together with sealing rings is clamped.

Fig. 3 zeigt deutlich, daß je nach Material, das mit der enzymhaltigen Reaktionsflüssigkeit in Kon­ takt komnt, deutlich unterschiedliche Desakti­ vierungsraten erhalten werden. Fig. 3 clearly shows that depending on the material that comes into contact with the enzyme-containing reaction liquid in Kon, significantly different deactivation rates are obtained.

Claims (2)

1. Enzymmembranreaktor mit einer Reaktionszelle, die von einer Ultrafiltrationsmembran mit Rückhaltever­ mögen für Enzyme abgeschlossen ist, über die der Reaktorablauf erfolgt, dadurch gekennzeichnet, daß die mit enzymhaltigem Reaktionsmedium in Kon­ takt kommenden Flächen - abgesehen von der Ultrafil­ trationsmembran - aus einem thermoplastischen Poly­ merisat bestehen, das frei von -C-S- oder -C-O-Grup­ pen ist.1. Enzyme membrane reactor with a reaction cell, which is completed by an ultrafiltration membrane with retainers for enzymes via which the reactor outlet takes place, characterized in that the surfaces coming into contact with the enzyme-containing reaction medium come into contact - apart from the ultrafiltration membrane - from a thermoplastic polymer exist that is free of -CS- or -CO groups. 2. Reaktor nach Anspruch 1, dadurch gekennzeichnet, daß das Polymerisat aus Vinylgruppen gebildet ist, bei denen R für H, CH3, Cl oder C6H5 steht.2. Reactor according to claim 1, characterized in that the polymer from vinyl groups is formed in which R is H, CH 3 , Cl or C 6 H 5 .
DE3937892A 1989-11-15 1989-11-15 Enzyme membrane reactor with ultrafiltration membrane - with surfaces of thermoplastic polymer free from Gps. interfering with protein structure of enzyme Granted DE3937892A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE3937892A DE3937892A1 (en) 1989-11-15 1989-11-15 Enzyme membrane reactor with ultrafiltration membrane - with surfaces of thermoplastic polymer free from Gps. interfering with protein structure of enzyme
CH3518/90A CH679676A5 (en) 1989-11-15 1990-11-05

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE3937892A DE3937892A1 (en) 1989-11-15 1989-11-15 Enzyme membrane reactor with ultrafiltration membrane - with surfaces of thermoplastic polymer free from Gps. interfering with protein structure of enzyme

Publications (2)

Publication Number Publication Date
DE3937892A1 DE3937892A1 (en) 1991-05-16
DE3937892C2 true DE3937892C2 (en) 1991-09-12

Family

ID=6393521

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3937892A Granted DE3937892A1 (en) 1989-11-15 1989-11-15 Enzyme membrane reactor with ultrafiltration membrane - with surfaces of thermoplastic polymer free from Gps. interfering with protein structure of enzyme

Country Status (2)

Country Link
CH (1) CH679676A5 (en)
DE (1) DE3937892A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8329459B2 (en) 2001-07-13 2012-12-11 Co2 Solutions Inc. Carbonic anhydrase system and process for CO2 containing gas effluent treatment

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19703031C2 (en) * 1997-01-29 1998-10-29 Forschungszentrum Juelich Gmbh Process for the production of NDP sugar
EP1521629B1 (en) * 2002-07-11 2007-02-07 CO2 Solution Inc. Triphasic bioreactor and process for gas effluent treatment

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8329459B2 (en) 2001-07-13 2012-12-11 Co2 Solutions Inc. Carbonic anhydrase system and process for CO2 containing gas effluent treatment
US8329458B2 (en) 2001-07-13 2012-12-11 Co2 Solutions Inc. Carbonic anhydrase bioreactor and process for CO2 containing gas effluent treatment
US8329460B2 (en) 2001-07-13 2012-12-11 CO2 Solutions, Inc. Carbonic anhydrase bioreactor and process

Also Published As

Publication number Publication date
DE3937892A1 (en) 1991-05-16
CH679676A5 (en) 1992-03-31

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Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
D2 Grant after examination
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee