DE3937892A1 - Enzyme membrane reactor with ultrafiltration membrane - with surfaces of thermoplastic polymer free from Gps. interfering with protein structure of enzyme - Google Patents

Abstract

In an enzyme membrane reactor with a cell closed by an ultrafiltration membrane which retains enzymes, over which the reactor effluent passes, surfaces, apart from the ultrafiltration membrane, coming into contact with reaction medium contg. enzymes, are of a thermoplastic polymer free from -C-S or -C-O gps. The polymer is formed of vinyl gps. of formula H2C=CHR. R = H, Me, Cl or Ph. ADVANTAGE - The synthetic is free from components which interfere with the protein structure of the enzymes, and the deactivation rate of enzymes, esp. of aldolases, is low.

Description

The invention relates to an enzyme membrane reactor with a reaction cell from a Ultrafiltration membrane with retention capacity for Enzymes through which the reactor is shut off run takes place.

Enzyme membrane reactors are for continuous Implementation of enzyme-catalyzed reactions of great interest. The behavior of such bio Catalysts vary depending on their particularity and becomes sensitive to the surrounding media influenced.

It has now been shown that not only that surrounds de medium itself, but also the wall material of the Reactor plays a role in the deactivation of the Reactor contained enzymes plays.

Such enzyme membrane reactors are usually used made of steel, especially stainless steel. For However, demonstration purposes have also been used Clear plastic cells (especially Plexiglass).

It has now been found that the deactivation gene speed especially of aldolases in particular is low if the reactor is made of a plastic  with a smooth hydrophobic surface, whose chemical composition as free of compo as possible is leading to disturbances in the protein structure of the enzyme can lead.

The enzyme membrane according to the invention then developed branch reactor of the type mentioned is characterized in that with enzyme-containing reaction medium coming into contact - apart from the ultrafiltration membrane - from a thermopla consist polymeric that is free of -C-S- or -C-O groups.

The polymer is preferably vinyli cal monomers of the formula

formed in which R is H, CH ₃ , Cl or C ₆ H ₅ .

The accompanying drawings serve for further ver illustration of the invention; show it:

Figure 1 schematically shows the structure of an EMR in section.

Figure 2 is a built EMR cell in an exploded view.

Fig. 3 shows the activity profile of an aldolase as a function of time when different materials are present.

Referring to FIG. 1, an EMR 1 comprises a trafiltrationsmembran by a Ul 2 completed Reaktionszel le 3, in which via one or more feed streams are fed 4 sub strate, while 5 of the reac torablauf going on. In the cell 3 there is the reaction liquid with the enzyme 6 soluble therein, which is moved by means of a magnetic stirrer 7 .

A practical embodiment nit flat membrane is shown in Fig. 2, according to which the cell is formed between end flanges 8 , 8 ', between which the side wall and the membrane together with sealing rings is clamped.

Fig. 3 clearly shows that depending on the material that comes into contact with the enzyme-containing reaction liquid in Kon, significantly different deactivation rates are obtained.

Claims (2)

1. Enzyme membrane reactor with a reaction cell, which is completed by an ultrafiltration membrane with retention properties for enzymes via which the reactor outlet takes place, characterized in that the surfaces coming into contact with the enzyme-containing reaction medium come into contact - apart from the ultrafiltration membrane - from a thermoplastic polymer exist that is free of -CS- or -CO groups. 2. Reactor according to claim 1, characterized in that the polymer from vinyl groups is formed in which R is H, CH ₃ , Cl or C ₆ H ₅ .