DE3900862A1 - Method of disinfecting and cleaning surfaces, and agent for performing the method - Google Patents

Abstract

For disinfection and cleaning of surfaces of articles which come into contact with live tissue, the surfaces must be contacted with germicidal agents. For this purpose, the articles are placed in a vessel which contains a sufficient concentration of the germicidal agent. By wetting the surfaces to be disinfected and cleaned the germicidal agent is permitted to act upon the contaminants for a sufficient period of time. According to the invention, the vessel contains, in addition to the germicidal agent, an agent which causes decomposition of the germicidal agent, the half-life of the decomposition being sufficiently long. The germicidal agent decomposes into other substances whose concentration together with the agent which causes decomposition on completion of the application does not affect live tissue. <IMAGE>

Description

The invention relates to a method for disinfection and Cleaning surfaces of one or more objects in a vessel with a germicidal agent present in the vessel the concentration required for disinfection and cleaning and which in the vessel at least to desinfi completely reaching decorative and clean surfaces, after which at least one of the disinfected and cleaned upper surfaces come into contact with living tissue and care products to carry out the procedures.

Care products for the disinfection and cleaning of surfaces, which come into contact with living tissue, should the upper free surfaces of microorganisms and possible deposits remove. To achieve this, you bring the surfaces with the care products contained in an aqueous solution in a close contact. After that they have to be used Care products are removed from the surfaces again, as they are have a destructive effect on the living tissue. In particular, in the case of contact lens care products, a distinction is made between chemical agents (chlorhexidine digluconate, Benzal potassium chloride, thiomersal, etc.) and hydrogen peroxide Systems.

A disadvantage of all these known means and systems for Cleaning and disinfecting contact lenses is theirs Adsorption in the contact lens material and thus the Storage of preservatives. For sensitive People can therefore cause eye irritation and allergies come, even if according to the instructions an afterthought Neutralization takes place. If this neutralization even forgotten damage to the eye can occur. The disadvantage is Furthermore, that the care must be done in two steps, where  a certain minimum time is required for each step.

The object of the invention is an application-friendly Desin fektions- and cleaning agent to create, which after the Care should no longer be neutralized by a second solution got to.

The object is achieved according to the characterizing part of the first patent claim, characterized in that in the Vessel in addition to the germicide means a means to off dissolving the decay of the germicidal agent, wherein the Half-life of decay is large enough, and that the Germicidal agent breaks down into other substances, which after Termination of disinfection and cleaning in their concentrations tion together with the means of triggering the decay are ineffective against living tissue.

With this method can be a disinfection and cleaning perform without a subsequent removal or Neutralization of the germicide in a separate Step must be taken. So this process is particular for the disinfection and cleaning of surfaces of counter conditions in which the germicidal agent is at least penetrates into the upper layer of the objects and only very difficult to remove or neutralize as z. B. in soft contact lenses is the case.

As a germicidal agent in the context of the invention, all are suitable Substances which in the presence of a trigger in a vessel fall into other substances, it must be ensured that after completion of the disinfection and cleaning then in Container existing substances in their concentration ineffective are opposite to living tissue.

Due to the decay of the germicide after the expiry of the Application time can not cause any toxic effects after  use, especially on living tissue. To enable a longer application time, the Desin Fektion and cleaning done in a vessel. It must ensure that the germicidal Cleaning and disinfection has the necessary concentration, because the decay is a chemical reaction. A chemical Reaction is temperature dependent, so that the beginning concentration of the germicidal agent must be so high that an effective disinfection and cleaning in a big way Temperature range is ensured. In addition, must sicherge be placed that in the vessel at least to desinfi decorating and clean surface of the object full is constantly wetted. It is beneficial if the germicidal Agent already at the beginning of the procedure together with the agent to trigger the decay on the object acts because then no further additions are made during the cleaning process have to.

Before carrying out the procedure must be the germicidal Means as well as the means for triggering the decay strictly from be separated to a sufficiently long storage time of the To ensure funds. Only when the Ver driving, the decay from the outside by the user becomes aware initiated. The means for triggering the decay may be a catalyst, which optionally solid is fixed in the vessel, or a solution, which then itself must be ineffective against living tissue.

Since the germicidal substances disintegrate during use, it is their possible acute toxicity only from subordinate Meaning and must be placed on the market when Dar be taken into account. More decisive is the toxicological significance of any by - products that may be found in the Application in the solution can arise and given if at the surfaces, which with the living Tissue come into contact, making it a local uptake through which living tissue can come. It must in the selection  of the germicide, it is minimal Quantities are, which are included, albeit given if daily. On the other hand, the tear fluid becomes one record a considerable part of the humidifying by-products and so to their dilution in subsequent evaporation or Slurry contribute.

As for the living tissue ineffective solution is useful the application of the method for disinfecting and cleaning the Surfaces of objects that live with human Tissue come into contact, especially a sterile physiological saline (0.9%). This solution can then add more ingredients in a known manner become.

An application-friendly implementation of the method results when the germicide in pre-dosed amount of the Solution is added. This pre-dosage can with a Drop dispensers are made on a storage bottle or by a Pharmaceutical form, which is the discrete withdrawal of a ensures a metered quantity (water-soluble capsules, Tablets, plastic tubes, etc.).

To a sufficiently strong initial concentration of the germicidal To get by, the amount should be for the living Tissue ineffective solution on the pre-dosed amount of germ be coordinated with the killing agent. A tin as a cleaning bag container, in which a well-visible measuring mark for the Amount of solution ineffective for the living tissue, facilitates the implementation of the method in the application.

As a germicidal agent are particularly suitable Dicarbonates, including other germicidal substances with a acceptable half-life for carrying out the procedures are suitable, which isolated a longer time (<1 year at storage temperature 25 ° C) does not ver Verify their effectiveness  lose. The dicarbonates are particularly suitable Dimethyl dicarbonate and diethyl dicarbonate. Both substances are So far, especially for chemical food preservation been used, for. B. for beverage sterilization. dimethyldicarbonate is known under the trade name Velcorin and is today home especially for the cold disinfection of non-alcoholic refreshments used to soak. During hydrolysis dimethyldicarbonate decomposes in methanol and carbon dioxide. The half lives are 40 min (at 10 ° C) to 8 min (at 30 ° C). To one to fast Prevent decay at high ambient temperatures without the dose To increase, the procedure is at high outside temperatures preferably cooled (eg in a refrigerator). After the decomposition of dimethyldicarbonate is not germicidal Effect more available. For sensitive people that can Methanol cause a slight irritation. By a small one Addition of sodium bicarbonate, sodium lactate, ammonium carbonate, etc. as a methanol blocker in the ineffective solution even this slight irritation can be prevented.

Diethyl dicarbonate is known by the trade name Baycovin and was formerly used for the sterilization of fruit juices, wine etc. used. During the hydrolysis, diethyl dicarbonate decomposes into Ethanol and carbon dioxide. When selecting the invalid Solution here is to make sure that these are not ammonium salts contain. Also advantageous is the application of the method for storage fluids from which the articles can be taken out directly, the surfaces of the Contact objects safely with living tissue can.

The dicarbonates have the general formula

where R denotes the radicals. For dimethyl dicarbonate R = CH ₃ and for diethyl dicarbonate R = C ₂ H ₅ .

Dicarbonates hydrolyze, using water in other substances disintegrate. Here is the anhydride moiety essential, since here the water separates the chain.

In the present hydrolysis water is the means for Triggering the decay. The effectiveness of dimethyl dicarbonate and diethyl dicarbonate is so high that even minimal doses are absolutely germicidal. The necessary lethal concentrations for the germs listed below amount to dimethyldi carbonate

yeasts mg / l Saccharomyces carlsbergensis (dust yeast) 100 Saccharomyces carlsbergensis (breakage yeast) 60 Saccharomyces diastaticus 200 Saccharomyces oviformis 100 Saccharomyces bailii 120 Saccharomyces cerevisiae 40 Saccharomyces uvarum 30 Saccharomyces pastorianus 100 Saccharomyces apiculatus 60 Saccharomyces globosum 40 Zygosaccharomyces priorianus 75 Rhodotorula mucilaginosa 50 Rhodotorula glutinosa 40 Rhodotorula rubra 200 Candida krusei 200 Pichia membranefaciens 40 Pichia farinosa 100 Torulopsis candida 100 Torulopsis versatilis 100 Torulopsis stellata 65 Torula utilis 240 Endomyces lactis 60 Kloeckera apiculata 40 Hansenula anomala 50

bacteria Acetobacter pastorianum 80 Acetobacter xylinum 300 Escherichia coli 400 Staphylococcus aureus 100 Pseudomonas aeruginosa 100 Lactobacterium buchneri 40 Lactobacillus pastorianus 300 Lactobacillus brevis 200 Pediococcus cerevisiae 300

Mold Penicillium glaucum 200 Byssochlamys fulva 100 Botrytis cinerea 100 Mucor racemosus 500 Fusarium oxysporum 100

and for diethyl dicarbonate

Minimal lethal concentration of diethyl dicarbonate

The decay rate is primarily of the Temperature dependent. For dimethyl dicarbonate are the following Half-lives known, at

10 ° C 40 min
20 ° C 15 min
30 ° C 8 min.

If the dicarbonates are hermetically sealed, they are Durable for at least 1 year if the optimal Storage temperature of 20-25 ° C (room temperature) complied with becomes.

Short-term heating, z. B. when transporting in summer Outdoor temperatures is harmless, but the Reduce overall storage capacity. Cold storage has against it  no adverse effect on stability. Because the Dicarbonates when used within a few hours or Minutes fall apart, are only the secondary products for the health safety important.

A comparative study with a preparation containing a 3% H ₂ O ₂ solution against six microorganism strains (Staph aureus, Staph epidermidis, Pseud aeruginosu, Serratio marcescens, Candida albicans, Aspergillus fumigatus) gave the following result:

The hydrogen peroxide-containing comparator obtained after four hours of absolute sterility, with the expected Reduk tion by five powers of ten from this preparation.

Dimethyl dicarbonate reached a kill of six Powers of ten after 5-10 minutes at a concentration of 1000 ppm (at 20 ° C ambient temperature). A sterility became reached after 30-60 minutes.

A particularly sensitive living tissue is in the eyes to find which with hard or soft contact lenses in Come in contact.

Contact lenses are very small ones to disinfect and too cleansing objects with a large surface, which with very sensitive areas of the human body a long Have contact. To the soft or hard contact lenses a to be able to use for a longer period of time, they must be regularly be cleaned. Even at low doses of the mentioned Dicar Bonate (100 ppm) are good results with contact lenses targets (at an application time of minutes), using a trade usual application at a dose ~1000-3000 ppm an application in a wide temperature range allows. Do you want this Increase temperature range to higher temperatures, so can the Dose also be increased.  

The solution for the dissolution and disinfection of contact lenses before saline for the solution of the dicarbonate may be added to one of the following indicators in a concentration of about 0.001%: alizarin or Bromkresolpurpur or Bromthymolblau. The indicator indicates the complete decomposition of the dicarbonate by color change: in aqueous solution, the diboronate decomposes in such a way that, in addition to traces of alcohol , gaseous CO ₂ , which dissolves immediately in the aqueous saline solution, is formed and the initially neutral pH. Value of the solution of about 7 moves into the acidic area. As a result of this pH change, the color of the indicator changes from blue to yellow (bromothymol blue) or from purple to yellow (bromocresol purple) or from red to yellow (alizarin) and thus indicates the complete decomposition of the dicarbonate - the contact lens can then be put on the eye.

The care products can except the methanol blockers in the Use of dimethyl dicarbonate also further, the Cleaning supporting substances are added. Be here in particular the enzyme cleaners (eg proteases) and the top surface cleaner (eg Tween 40, SPAN, MYRJ, BRIJ, etc.) thinks that in low concentrations on the eye no Cause irritation.

The invention can be presented in various forms become. Forms of preparations of the invention for the care of Contact lenses are described in more detail below. The im The following application examples described relate in particular for use as a care product for Contact lenses, with quantities for other applications must be changed accordingly. Show it:

. Figure 1 is an administration with dispenser;

Fig. 2 shows a presentation with plastic tubes;

Fig. 3 is an administration with effervescent tablet;

Fig. 4 shows a presentation with capsule.

In Fig. 1, a contact lens cleaning set is shown. This set consists of a contact lens container ( 1 ), which has a very visible mark ( 2 ) on its inner wall ( 3 ). Up to this mark ( 2 ) the contact lens container ( 1 ) is filled with a physiological saline solution (0.9%). The contact lens container ( 1 ) has a window ( 4 ) made of a transparent plastic through which the mark ( 2 ) is clearly visible. The contact lens ( 6 ), which may be a hard or soft contact lens ( 6 ), is inserted into the plastic basket ( 5 ), whereupon the holder ( 5 ) is closed in a known manner. The holder ( 5 ) is located on an extension ( 8 ) of a lid ( 9 ) and is designed so that it allows the least possible access of the liquid cleaning solution in the contact lens container ( 1 ). Before the lenses ( 6 ) are introduced into the saline solution in the holder ( 5 ) and the cleaning container ( 1 ) is closed with the lid ( 9 ), a drop ( 11 ) (about 100 μl) of diethyl is added to the saline solution - or dimethyl dicarbonate in concentrated form containing solution of a sealed dispenser ( 10 ). In this solution from the dispenser ( 10 ) is also an indicator for measuring the pH. The concentration of the solution from the dispenser ( 10 ) is such that a dicarbonate concentration of <500 ppm and the concentration of the indicator about 0.001% in the saline solution safely reached.

After the drop from the dispenser ( 10 ) has been added to the saline solution in the contact lens container ( 1 ), insert the contact lenses ( 6 ) in the plastic basket ( 5 ) into the resulting solution and seal the contact lens container ( 1 ) with the lid ( 9 ). Thereafter, shake the contact lens container ( 1 ) several times, so that a homogeneous solution is formed and leaves the closed contact lens container ( 1 ) overnight, but at least for 2 hours at a room temperature between 20 and 25 ° C.

Then remove the contact lens ( 6 ) from the plastic basket ( 5 ) on the lid ( 9 ) of the contact lens container ( 1 ) and can put them directly on the eye. Before you can rinse the contact lens with a pure saline solution. However, this step is not absolutely necessary because the substances in the solution after purification are ineffective in their concentration in relation to living tissue. The remaining solution is poured away and rinsed the contact lens container ( 1 ) and the plastic basket ( 5 ) with pure saline again.

A particular problem of the substances according to the invention is their very high germicidal effect in the concentrated state. These makes a free donation in bulk for the broad publi problematic. It is therefore advantageous to the invention appropriate substances in quantities specific to the application administer.

One way a dicarbonate solution ( 12 ) of a saline solution in a contact lens container ( 13 ) in predosed amount in accordance with Fig. 2 is to weld the dicarbonate solution ( 12 ) in a plastic tube ( 14 ). The contact lenses ( 15 , 15 a ) are inserted into the plastic basket ( 16 , 16 a ) on the extension ( 17 ) of the lid ( 18 ) and ver closed. Thereafter, they are introduced into the saline solution (0.9%) filled contact lens container ( 13 ), whereupon this is firmly closed with the lid ( 18 ). In the lid ( 18 ) there is an opening ( 22 ) through which the plastic tube ( 14 ) with the dicarbonate solution ( 12 ) in the cleaning container ( 13 ) to a predetermined depth can be inserted, since the plastic tube ( 14 ) in its upper part a bead ( 21 ), with which it provides for a seal from the opening ( 22 ) in the lid ( 18 ). In the plastic tube (14) is a needle (19), which protrudes with its thickened end (20) of the plastic tube (14).

Before use, the retaining ring ( 21 a ) must be removed. By pressing on the thickened end ( 20 ) causes the needle ( 19 ) to pierce the plastic tube ( 14 ) so that the dicarbonate solution ( 12 ) in the saline in the contact lens container ( 13 ) leaks and mixes with the saline solution. By repeatedly shaking the Kontaktlinsenbe container ( 13 ) can accelerate the mixing process. It must in any case be ensured that the dicarbonate has a concentration of 500 ppm and the pH indicator contained in the dicarbonate solution ( 12 ) has a concentration of approximately 0.001% in the saline solution. In order to be able to see the color change of the pH indicator well at the end of the cleaning process, the cleaning container ( 13 ) or the lid ( 18 ) has a transparent window ( 11 ) at at least one point. After the color change is done after about 2 hours at room temperature, the contact lens ( 15 ) can be easily taken from the plastic cups ( 15 ) and placed directly on the eye, where you can rinse it with pure saline previously.

Instead of bumping the plastic tube ( 14 ), the dicarbonate solution ( 12 ) can be released with pH indicator with appropriate Konstrukion the plastic tube by rotating it.

The contact lens container ( 24 ) in Fig. 3 has a measuring mark ( 25 ) which ensures that always the same amount of saline solution in the contact lens container ( 24 ) is filled, so that at room temperature always the same amount of dicarbonate is needed. The amount of the pH indicator can always remain the same. This predosing can also be carried out by microcapsulating the cleaning solution with the aid of a water-soluble plastic and storing it as granules, in particular in the form of a capsule ( 26 ). A supply pack ( 27 ) may contain several capsules ( 26 ). The storage package ( 27 ) has a plastic shell with bulges ( 26 a ) in which the capsules ( 26 ) are inserted. These bulges are airtight sealed by a plastic-coated aluminum foil on the other side (as is usual in the drug packaging). By pressure on the bulge ( 26 a ) can be achieved that only one capsule ( 26 ) pierces the aluminum foil, while the other capsules ( 26 ) remain packed airtight.

After having removed a capsule ( 26 ) from the supply pack ( 27 ), the capsule can be placed in a space ( 28 ) between the plastic cups ( 32 ), which can be opened on one side thereof. The lid ( 29 ) which is used to seal the space ( 28 ) has very large free openings and serves as a contact surface for the contact lens ( 30 ), which by a second lid ( 31 ) with large openings in the plastic basket ( 32 ). is held. The stability of the lid ( 29 , 31 ) is achieved by stable webs ( 33 a , b ) made of plastic, which ensure that the contact lens ( 30 ) in the plastic basket ( 32 ) suffers no mechanical damage.

The capsule ( 26 ) is inserted with the contact lenses ( 30 ) together in the pre-dosed saline solution and the contact lens container ( 24 ) with the lid ( 34 ) firmly closed. Through a transparent opening ( 35 ) in the contact lens container ( 24 ) one can see the color change of the pH indicator, which indicates the complete decomposition of the dicarbonate. Thereafter, the contact lens ( 30 ) can be removed for use.

In the application example of Fig. 4 the dicarbonate with a neutral water-soluble tabletting known type (eg., Polyvinylpyrrolidone, starch, cellulose derivatives, etc.) is compressed into a tablet (36). For faster dissolution of the tablet ( 36 ) the tablet has been added to a mixture of citric acid and sodium bicarbonate (effervescent effect). These tablets ( 36 ) are taken from a supply pack ( 37 ) in which the tablets ( 36 ) lie in discrete bulges ( 36 a ) of a plastic shell, which is hermetically sealed on the other side with a plastic-coated aluminum foil.

The contact lenses ( 40 ) are inserted into the plastic baskets ( 41 ) which are fastened to the cover ( 42 ). After the tablet ( 36 ) has been placed in the saline solution filled up to the filling line ( 39 ) of the contact lens container ( 38 ), the contact lenses ( 40 ) are introduced into the plastic baskets ( 41 ) in the saline solution and the contact lens container ( 38 ) is closed with the Cover ( 42 ). Through two viewing holes ( 44 ) in the lid ( 42 ) you can see when the pH indicator turns over and the end of the cleaning process is reached. Due to the bubbling effect of the tablet ( 36 ), the cleaning effect is still supported.

Claims (23)

1. A method for disinfecting and cleaning surfaces of one or more objects in a vessel with a germicidal agent, which in the vessel fect for disinfection and cleaning necessary concentration and which completely reaches in the vessel at least the surface to be disinfected and cleaned, according to which at least one disinfected and cleaned surface comes into contact with living tissue, characterized in that a means for inducing disintegration of the germicidal agent is added to a vessel in addition to the germicidal agent, the half-life of the disintegration being sufficiently great; germicidal agent is decomposed into other substances which, after completion of the disinfection and purification in their concentration together with the means for initiating the disintegration are ineffective against living tissue. 2. The method according to claim 1, characterized in that as Means of triggering the decay one for the living Tissue ineffective solution is used. 3. The method according to claim 2, characterized in that as for the living tissue ineffective solution a sterile physiological saline (0.9%) is used. 4. The method according to claim 1 or 2, characterized that the germicidal agent in pre-dosed amount of the solution is added. 5. The method according to any one of claims 1-4, characterized records that a dicarbonate is used as a germicide is used. 6. The method according to claim 5, characterized in that as  Germicidal agent dimethyl dicarbonate is used. 7. The method according to claim 5, characterized in that as Germicidal agent diethyl dicarbonate is used. 8. The method according to any one of claims 1-7, characterized by use for disinfecting and cleaning of hard or soft contact lenses. 9. Use of dimethyl dicarbonate for disinfection and Cleaning contact lenses. 10. Use of diethyl dicarbonate for disinfection and Cleaning contact lenses. 11. Care products for the disinfection and cleaning of surfaces one or more objects in a vessel with a germicidal agent, which in the vessel to Desin fektion and cleaning has the necessary concentration and which in the vessel at least to be disinfected and completely achieved surface to be cleaned, after which at least one disinfected and cleaned surface with living tissue comes into contact, characterized that the germicidal remedy with a sufficiently large half value of decay in the presence of a trigger in the Vessel decomposes into other substances, which after completion of the Disinfection and purification together in their concentration with the means for triggering the decay ineffective against are about living tissue. 12. Care agent according to claim 11, characterized in that the trigger is a sterile physiological saline solution (0.9%). 13. Care agent according to one of claims 11 or 12, characterized characterized in that the germicidal agent is a dicarbonate  is. 14. Care agent according to claim 13, characterized in that the dicarbonate is dimethyl dicarbonate. 15. Care agent according to claim 13, characterized in that the dicarbonate is diethyl dicarbonate. 16. care agent according to any one of claims 11-15, characterized ge indicates that in the care agent an indicator ent Keep the process of disinfection and cleaning Visually displays. 17. care agent according to any one of claims 11-16, characterized ge indicates that in the care agent, a methanol blocker is available. 18. care agent according to any one of claims 11-17, characterized ge indicates that in the care agent, a surface or Enzyme cleaner is present, its concentration ineffective is opposite to living tissue. 19. Care agent according to any one of claims 11-18, characterized characterized in that the care agent in tablet form is present. 20. Care agent according to any one of claims 11-19, characterized characterized in that the care agent in a Plastic tube is welded, the Care product only in the cleaning container from the Plastic tube escapes. 21. Care agent according to any one of claims 11-20, characterized characterized in that the care agent in a Drop dispenser is included. 22. Care agent according to one of claims 11-21, characterized  characterized in that the concentration of the dicarbonate to Start of care in the aqueous solution not more than 1000 ppm. 23. Care agent according to one of claims 11-22, characterized by the application for disinfection and cleaning of hard or soft contact lenses.